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Peng, Y. Batch Fermentation Techniques. Encyclopedia. Available online: https://encyclopedia.pub/entry/61406 (accessed on 12 November 2026).
Peng Y. Batch Fermentation Techniques. Encyclopedia. Available at: https://encyclopedia.pub/entry/61406. Accessed November 12, 2026.
Peng, Yu. "Batch Fermentation Techniques" Encyclopedia, https://encyclopedia.pub/entry/61406 (accessed November 12, 2026).
Peng, Y. (2026, October 25). Batch Fermentation Techniques. In Encyclopedia. https://encyclopedia.pub/entry/61406
Peng, Yu. "Batch Fermentation Techniques." Encyclopedia. Web. 25 October, 2026.

Batch Fermentation Techniques

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Batch fermentation techniques are procedures for carrying out a discrete cultivation run from inoculation to harvest without a continuous replacement of culture broth [1]. A defined volume of medium is charged before inoculation, and the culture then progresses through lag, growth, production, and decline phases as nutrients are consumed and metabolites accumulate [1][2]. The technique includes preparation and sterilization of the medium, inoculum transfer, regulation of temperature and pH, control of aeration and agitation when required, sampling, and selection of a harvest criterion [2]. In a strict batch, no feed is added after inoculation, although pH-control reagents, antifoam, or gases may still enter the vessel. The resulting time trajectory reflects the starting composition, inoculum physiology, vessel environment, and organism-specific responses to nutrient depletion and product inhibition [1][3]. Batch operation is widely used for screening, seed-train development, food and beverage fermentations, and processes in which a reproducible finite run is preferable to long-term steady-state operation [4].

batch culture inoculum development finite cultivation harvest criterion
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References

  1. Peter F. Stanbury; Allan Whitaker; Stephen J. Hall. Principles of Fermentation Technology; Elsevier: Amsterdam, NX, Netherlands, 2017. [CrossRef]
  2. Christopher J. Hewitt; Alvin W. Nienow. The Scale‐Up of Microbial Batch and Fed‐Batch Fermentation Processes; Elsevier: Amsterdam, NX, Netherlands, 2007; pp. 105-135. [CrossRef]
  3. Fadzlie Wong Faizal Wong; Murni Halim; Arbakariya B. Ariff. Extractive Fermentation Employing Ion-Exchange Resin to Enhance Cell Growth and Production of Metabolites Subject to Product or By-Product Inhibition; IntechOpen: Rijeka, Croatia, 2018. [CrossRef]
  4. Pragati Singh; Niharika Arya; Shalini Sharma; Ankita Sharma; Prasad Rasane; Supriya Singh Gaur; Sawinder Kaur; Mahendra Gunjal; Hasan Kiliçgün; Jyoti Singh; A comprehensive review of fermentation technologies in food processing: from traditional to cutting edge. Turk. J. Agric. For. 2025, 49, 448-467. [CrossRef]
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Subjects: Food Science & Technology
Contributor MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register : Yu Peng
View Times: 8
Entry Collection: Biosynthesis and Synthetic Biology
Revisions: 2 times (View History)
Update Date: 25 Oct 2026
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