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Peng, Y. Recombinant DNA. Encyclopedia. Available online: https://encyclopedia.pub/entry/61069 (accessed on 02 November 2026).
Peng Y. Recombinant DNA. Encyclopedia. Available at: https://encyclopedia.pub/entry/61069. Accessed November 02, 2026.
Peng, Yu. "Recombinant DNA" Encyclopedia, https://encyclopedia.pub/entry/61069 (accessed November 02, 2026).
Peng, Y. (2026, October 17). Recombinant DNA. In Encyclopedia. https://encyclopedia.pub/entry/61069
Peng, Yu. "Recombinant DNA." Encyclopedia. Web. 17 October, 2026.

Recombinant DNA

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Recombinant DNA is a DNA molecule formed by joining sequence segments into a new genetic arrangement. Complementary ends can align the constituent fragments, and polymerase and ligase activities complete a continuous DNA backbone [1]. Constructs can combine promoters, coding sequences, and termination elements into single or multiple expression units, with the orientation and boundaries of each segment specified during assembly [2]. Restriction-based joining uses recognition and cleavage properties that generate compatible fragment ends; controlled methylation can provide additional specificity for cleavage and ligation [3]. Hierarchical assembly connects intermediate constructs into larger molecules while preserving the intended sequence organization [4]. The resulting molecule carries the combined nucleotide information, including the designed junctions and any replication or selection elements included in the construct [5].

DNA assembly plasmid constructs sequence junctions expression constructs
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References

  1. Daniel G Gibson; Lei Young; Ray-Yuan Chuang; J Craig Venter; Clyde A Hutchison; Hamilton O Smith; Enzymatic Assembly of DNA Molecules up to Several Hundred Kilobases. Nat. Methods 2009, 6, 343-345. [CrossRef]
  2. George M Taylor; Paweł M Mordaka; John T Heap; Start-Stop Assembly: a Functionally Scarless DNA Assembly System Optimized for Metabolic Engineering. Nucleic Acids Res. 2018, 47, e17-e17. [CrossRef]
  3. Wei-Hua Chen; Zhong-Jun Qin; Jin Wang; Guo-Ping Zhao; The MASTER (methylation-assisted tailorable ends rational) Ligation Method for Seamless DNA Assembly. Nucleic Acids Res. 2013, 41, e93-e93. [CrossRef]
  4. Da Lin; Christopher A O’cAllaghan; MetClo: Methylase-assisted Hierarchical DNA Assembly Using a Single Type IIS Restriction Enzyme. Nucleic Acids Res. 2018, 46, e113-e113. [CrossRef]
  5. Fang Ba; Qing Sun; Site-specificRecombinases and Their Essential Role in Synthetic Biology. Biotechnol. Adv. 2026, 92, 109005. [CrossRef]
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Subjects: Biochemistry & Molecular Biology
Contributor MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register : Yu Peng
View Times: 3
Entry Collection: Molecular Biology and Gene Expression Regulation
Revisions: 2 times (View History)
Update Date: 24 Oct 2026
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