Your browser does not fully support modern features. Please upgrade for a smoother experience.
Submitted Successfully!
+1 credit
Thank you for your contribution! You can also upload a video entry or images related to this topic.
For video creation, please contact our Academic Video Service.
Video Upload Options
We provide professional Academic Video Service to translate complex research into visually appealing presentations. Would you like to try it?
Cite
If you have any further questions, please contact Encyclopedia Editorial Office.
Peng, Y. Biotin-Dependent Enzymes. Encyclopedia. Available online: https://encyclopedia.pub/entry/61056 (accessed on 12 November 2026).
Peng Y. Biotin-Dependent Enzymes. Encyclopedia. Available at: https://encyclopedia.pub/entry/61056. Accessed November 12, 2026.
Peng, Yu. "Biotin-Dependent Enzymes" Encyclopedia, https://encyclopedia.pub/entry/61056 (accessed November 12, 2026).
Peng, Y. (2026, October 17). Biotin-Dependent Enzymes. In Encyclopedia. https://encyclopedia.pub/entry/61056
Peng, Yu. "Biotin-Dependent Enzymes." Encyclopedia. Web. 17 October, 2026.
Copy Citation
Biotin-Dependent Enzymes
EditBiotin-dependent enzymes are enzymes that use covalently attached biotin to transfer a carboxyl group during catalysis. The biotin cofactor is linked to a lysine residue within a carrier region of the protein and moves between distinct catalytic sites [1]. In biotin-dependent carboxylases, one site uses ATP and bicarbonate to carboxylate biotin, and another transfers the carboxyl group to an organic acceptor [2]. Domain movements carry this activated intermediate between the sites; in multimeric enzymes, the participating sites can lie on different polypeptide chains [3][4]. The holoenzyme can distribute biotin carboxylase, carrier, and carboxyltransferase functions among separate subunits. Their assembly positions the mobile biotin-bearing domain for successive reactions and links cofactor movement to substrate-specific carbon–carbon bond formation [5].
biotinylation
carboxylases
biotin protein ligase
covalent cofactor
Help expand this entry
Share your expertise by adding detailed content and references to improve this entry.
Add content
References
- Moritz Hunkeler; Anna Hagmann; Edward Stuttfeld; Mohamed Chami; Yakir Guri; Henning Stahlberg; Timm Maier; Structural Basis for Regulation of Human Acetyl-CoA Carboxylase. Nature 2018, 558, 470-474. [CrossRef]
- Liang Tong; Structure and Function of Biotin-dependent Carboxylases. Cell. Mol. Life Sci. 2012, 70, 863-891. [CrossRef]
- Alejandro D. Meruelo; Seong Kyu Han; Sanguk Kim; James U. Bowie; Structural Differences Between Thermophilic and Mesophilic Membrane Proteins. Protein Sci. 2012, 21, 1746-1753. [CrossRef]
- Martin St. Maurice; Laurie Reinhardt; Kathy H. Surinya; Paul V. Attwood; John C. Wallace; W. Wallace Cleland; Ivan Rayment; Domain Architecture of Pyruvate Carboxylase, a Biotin-Dependent Multifunctional Enzyme. Science 2007, 317, 1076-1079. [CrossRef]
- Christine S. Huang; Kianoush Sadre-Bazzaz; Yang Shen; Binbin Deng; Z. Hong Zhou; Liang Tong; Crystal Structure of the α6β6 Holoenzyme of Propionyl-Coenzyme A Carboxylase. Nature 2010, 466, 1001-1005. [CrossRef]
More
Information
Subjects:
Biochemistry & Molecular Biology
Contributor
MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register
:
View Times:
6
Entry Collection:
Biosynthesis and Synthetic Biology
Revisions:
2 times
(View History)
Update Date:
24 Oct 2026
Table of Contents
Notice
You are not a member of the advisory board for this topic. If you want to update advisory board member profile, please contact office@encyclopedia.pub.
OK
Confirm
Only members of the Encyclopedia advisory board for this topic are allowed to note entries. Would you like to become an advisory board member of the Encyclopedia?
Yes
No
${ textCharacter }/${ maxCharacter }
Submit
Cancel
Back
Comments
${ item }
|
${ item.createdUser.fullName }
${ item.createdAt }
${ item.vote }
${ item.reply }
Delete
${ reply.createdUser.fullName }
${ reply.createdAt }
${ reply.vote }
Delete
There is no reply to this comment~
${ item.replyTextCharacter }/${ item.replyMaxCharacter }
Submit
Cancel
More
No more~
There is no comment~
${ textCharacter }/${ maxCharacter }
Submit
Cancel
${ selectedItem.replyTextCharacter }/${ selectedItem.replyMaxCharacter }
Submit
Cancel
Confirm
Are you sure to Delete?
Yes
No