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Peng, Y. Recombinant Protein Tagging. Encyclopedia. Available online: https://encyclopedia.pub/entry/60952 (accessed on 12 November 2026).
Peng Y. Recombinant Protein Tagging. Encyclopedia. Available at: https://encyclopedia.pub/entry/60952. Accessed November 12, 2026.
Peng, Yu. "Recombinant Protein Tagging" Encyclopedia, https://encyclopedia.pub/entry/60952 (accessed November 12, 2026).
Peng, Y. (2026, October 14). Recombinant Protein Tagging. In Encyclopedia. https://encyclopedia.pub/entry/60952
Peng, Yu. "Recombinant Protein Tagging." Encyclopedia. Web. 14 October, 2026.
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Recombinant Protein Tagging
EditRecombinant protein tagging is the genetic fusion of a peptide or protein sequence to a target protein, producing a polypeptide with an additional recognition or functional element. Tags can provide binding sites and can alter the solubility, yield, or folding of the fusion protein [1]. An affinity tag interacts with a corresponding ligand, allowing the tagged product to be selectively captured from a mixture [2]. Tag sequences can also encode epitopes, localization reporters, or elements that recruit cellular activities; their placement must preserve the reading frame of the target coding sequence [3]. In split-fluorescent-protein systems, a short fused fragment associates with a complementary fragment to assemble a fluorescent complex, linking detection to the presence of the tagged protein [4].
fusion protein
peptide tag
linker
recombinant expression
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References
- David S. Waugh; Making the Most of Affinity Tags. Trends Biotechnol. 2005, 23, 316-320. [CrossRef]
- Ana Sofia Pina; Christopher R. Lowe; Ana Cecília A. Roque; Challenges and Opportunities in the Purification of Recombinant Tagged Proteins. Biotechnol. Adv. 2014, 32, 366-381. [CrossRef]
- Giel Vandemoortele; Sven Eyckerman; Kris Gevaert; Pick a Tag and Explore the Functions of Your Pet Protein. Trends Biotechnol. 2019, 37, 1078-1090. [CrossRef]
- Stéphanie Cabantous; Thomas C Terwilliger; Geoffrey S Waldo; Protein Tagging and Detection with Engineered Self-assembling Fragments of Green Fluorescent Protein. Nat. Biotechnol. 2005, 23, 102-107. [CrossRef]
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Biochemistry & Molecular Biology
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