Protein engineering is the intentional design, generation, and characterization of proteins with specified molecular properties. It connects changes in amino acid sequence or molecular architecture with changes in structure, dynamics, binding, stability, or catalytic behavior ^[1]. Engineered proteins may contain substitutions, insertions, deletions, or redesigned arrangements of structural elements. Sequence changes can be chosen using structural and mechanistic information, generated within variant libraries, or proposed through computational models ^[1][2]. The resulting molecules are evaluated experimentally to establish their properties under defined conditions ^[1][3]. The relevant molecular context includes folding, interactions among residues, active-site organization, and interactions with ligands or other macromolecules ^[3][4]. Consequently, the effect of a sequence change depends on the surrounding protein framework. Protein engineering comprises the coordinated relationship between a molecular specification, sequence-level design or diversification, and characterization of the resulting protein ^[3].