Site-directed mutagenesis is the deliberate introduction of a specified sequence alteration at a selected position or region of a DNA molecule. The intended alteration is encoded in a designed nucleic acid reagent and incorporated into newly synthesized or reconstructed DNA. Changes can include individual nucleotide substitutions, short insertions or deletions, and replacement of defined sequence segments ^[1][2]. In common polymerase-based formats, mutation-bearing primers anneal to a DNA template and direct synthesis of molecules containing the designed sequence. Primer complementarity, template sequence composition, polymerase properties, and subsequent processing influence the composition of the resulting DNA population ^[3]. The molecular endpoint is a DNA construct carrying the intended change within an otherwise specified sequence context ^[1][3][4]. Sequence analysis establishes the identity of that construct and detects unintended alterations that may accompany its generation ^[1][3].