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Kalinková, L.; Sevcikova, A.; Stevurkova, V.; Fridrichova, I.; Ciernikova*, S. DNA Methylation in Leukemia, Myelodysplastic Syndrome, and Lymphoma. Encyclopedia. Available online: https://encyclopedia.pub/entry/56575 (accessed on 21 May 2024).
Kalinková L, Sevcikova A, Stevurkova V, Fridrichova I, Ciernikova* S. DNA Methylation in Leukemia, Myelodysplastic Syndrome, and Lymphoma. Encyclopedia. Available at: https://encyclopedia.pub/entry/56575. Accessed May 21, 2024.
Kalinková, Lenka, Aneta Sevcikova, Viola Stevurkova, Ivana Fridrichova, Sona Ciernikova*. "DNA Methylation in Leukemia, Myelodysplastic Syndrome, and Lymphoma" Encyclopedia, https://encyclopedia.pub/entry/56575 (accessed May 21, 2024).
Kalinková, L., Sevcikova, A., Stevurkova, V., Fridrichova, I., & Ciernikova*, S. (2024, April 09). DNA Methylation in Leukemia, Myelodysplastic Syndrome, and Lymphoma. In Encyclopedia. https://encyclopedia.pub/entry/56575
Kalinková, Lenka, et al. "DNA Methylation in Leukemia, Myelodysplastic Syndrome, and Lymphoma." Encyclopedia. Web. 09 April, 2024.
DNA Methylation in Leukemia, Myelodysplastic Syndrome, and Lymphoma
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DNA methylation represents a crucial mechanism of epigenetic regulation in hematologic malignancies. The methylation process is controlled by specific DNA methyl transferases and other regulators, which are often affected by genetic alterations. Global hypomethylation and hypermethylation of tumor suppressor genes are associated with hematologic cancer development and progression. Several epi-drugs have been successfully implicated in the treatment of hematologic malignancies, including the hypomethylating agents (HMAs) decitabine and azacytidine. However, combinations with other treatment modalities and the discovery of new molecules are still the subject of research to increase sensitivity to anti-cancer therapies and improve patient outcomes. 

hematologic malignancies epigenetic regulation DNA methylation

1. Introduction

Hematologic malignancies form a heterogeneous group of acute and chronic diseases, clonally expanding into the blood, bone marrow, and lymph nodes. Due to their highly aggressive manner, blood cancers are characterized by rapid progress, affecting both pediatric and adult patients. Improved treatment response and outcomes have been documented in pediatric patients, who achieve remission in the overwhelming majority. Genetic and epigenetic changes lead to the clonal proliferation of stem and progenitor cells. Alterations in downstream signaling pathways contribute to the disruption of the self-renewal ability of hematopoietic cells and their differentiation into other lineages.
Although epigenetic mechanisms have been largely evaluated in embryonic development, differentiation, and organogenesis [1][2], mounting evidence supports their critical role in cancer development and treatment. In a very recent concept, aberrant epigenetic programming is one of the hallmarks of malignant progression [3]. The process of DNA methylation is the most studied epigenetic mechanism in both normal and cancer cells, and its correct regulation is crucial for the transcription of different regulating genes, maintaining genome integrity, and proper immune responses [4]. Genetic changes in DNA methylation enzymes and regulators together with abnormal DNA methylation are associated with cancer-promoting changes [5]. In addition to gene silencing by promoter hypermethylation at CpG islands, DNA genome hypomethylation is a typical sign of human cancers [4].
DNA methylation plays a critical role in hematopoiesis and hematopoietic stem cell differentiation and proliferation [6]. Epigenetic modulation by DNA methylation is involved in several stages, including blood cell lineaging and the formation of the final cell types [7]. According to the findings, aberrant DNA methylation patterns during hematopoiesis linked to the dysfunction of DNA methylation-related enzymes often lead to blood cancer development [8]. With heterogeneity and different clinical severity of individual subtypes, it is more than inevitable that investigations into new diagnostic and therapeutic possibilities are performed.

2. DNA Methylation of Target Genes in Leukemias, Myelodysplastic Syndromes, and Lymphomas

Genetic changes in regulators of the DNA methylation process can cause alterations in genome-methylation levels in the form of a loss of global methylation. On the other hand, almost intact global methylation was found in several hematologic malignancies. Genome-wide methylation studies in acute myeloid leukemia (AML), chronic myeloid leukemia (CML), chronic lymphocytic leukemia (CLL), acute lymphoblastic leukemia (ALL), and lymphomas described different regions with aberrantly methylated genes, which could help to elucidate the malignant transformation process and find potential targets for diagnosis, prognosis, or therapy of hematological cancers [9][10][11][12][13][14].

2.1. Aberrant Methylation in MDS and AML

Myelodysplastic syndrome (MDS) and AML belong to the group of hematologic malignancies characterized by clonal hematopoiesis. Both diagnoses share similar clinical and pathologic features, but they differ in the percentage of blasts in peripheral blood and bone marrow. DNA methylation of different target genes has been described in patients with MDS and acute/chronic leukemia (Figure 1).
Figure 1. Methylation-affected genes associated with leukemias and myelodysplastic syndromes. DNA methylation plays a key role in the initiation and progression of hematological malignancies. DNMTs catalyze transferring of the methyl group (a red circle) to the 5-carbon position of cytosine within CpG dinucleotides in DNA sequence (each strand of double-stranded DNA is marked by different colors, yellow and blue), leading to the formation of 5-methyl-cytosine. Abnormal methylation patterns in bone marrow cells may predict responsiveness to the treatment. A panel of aberrantly methylated genes shown to have diagnostic and prognostic value. Targeting the hypermethylated promoters of tumor suppressor genes might represent a perspective trend for hypomethylating drug therapy alone or in combination. Abbreviation: DNMTs, DNA methyltransferases.
The HOX gene family represents the most studied genes, which are regulated by DNA methylation in AML. In an extensive study, Gao et al. identified 29 genes whose expression correlated with differently methylated CpG sites. Within the HOX family, high methylation was found in HOXA7, HOXA9, HOXA10, and HOXB3 genes [9]. Similar results were found in mesenchymal stromal cells from bone marrow in MDS and AML patients, where preferentially aberrant methylated genes were HOXA1, HOXA4, HOXA5, HOXA9, HOXA10, HOXA11, HOXB5, HOXC4, and HOXC6 [15].
Promoters of many tumor suppressor genes (TSGs) are most commonly inhibited by methylation. Cyclin-dependent kinase inhibitors p15 and p16, important in the regulation of proliferation, are frequently methylated in AML patients. Their higher methylation was associated with lower (overall survival) OS, recurrence-free survival (RFS), and frequency of CR [16][17][18]. In AML and MDS patients, the relationship between gene methylation status and shorter OS was reported for ERα, OLIG2, ITGBL1, SCIN, DLX5, MSH2, RAD50, and SOCS1 genes [19][20][21][22][23][24].
The DOK protein family, characterized as phosphotyrosine adapters with several functions in cell biology, is commonly expressed in myeloid cells. In a study comprising AML patients, He et al. observed that hypermethylation-mediated decreased expression of DOK1 and DOK2 genes was associated with lower OS [25]. Similarly, the higher methylation status of another member DOK6 gene was found in AML patients, but with the opposite effect on OS [26]. Longer OS was associated with higher promoter methylation in other genes, including C1R and DNA repair genes MLH1 and RAD51 [23][27].
Sestakova et al. performed an extensive validation study for 27 genes from 14 studies to verify the predictive role of aberrant DNA methylation in AML. The results showed that hypermethylation of CEBPA, PBX3, LZTS2, and NRGA1 serves as a predictor for longer survival [28]. In addition, higher methylation of GPX3 and DLX4 correlated with a favorable treatment impact, which is in contrast to previously reported studies documenting their correlation with lower OS [29][30].
In some cases, mutations in important transcription factors can lead to alterations in epigenetic modifications. Genetic changes in Runt-related transcription factor 1 (RUNX1), a key player in hematopoiesis, led to aberrant methylation in target genes. The study on the RUNX1-mutated AML cohort identified 51 differential methylated genes. As shown, the changes in expression profiles were found in ten of them. Hypermethylation of CD96, LTK, and MS4A3 correlated with poor prognosis, which could be related to their effects on cell cycle regulation and differentiation [31]. Higher methylation of SOX7, TCF21, CTNNA1, and CAMK4, as well as hypomethylation of DDX43, LZTS2, or NRGA1, was observed in MDS/AML patients. Due to the lack of prognostic potential in numerous studies, complex evaluation requires further investigation [32][33][34][35][36][37].

2.2. Aberrant Methylation in CML

CML is characterized by the presence of translocation-creating oncoprotein BCR-ABL1, and the development of this disease consists of three phases, namely, the chronic phase (CP), accelerated phase (AP), and blast crisis phase (BP) [38]. The high content of aberrantly methylated CpG compared to healthy donors was found through a methylome analysis of CML patients. Moreover, the number of abnormally methylated CpG increased from CP to the BP phase. Most of the CpG sites with increased methylation (88%) were located in GpG islands or in the very close region, which overlapped with 348 genes in the peripheral blood of BP patients [10].
Jelinek et al. observed that among 10 selected genes analyzed by pyrosequencing, ABL1, CDH3, and NPM2 presented the highest methylation in all phases of CML. Furthermore, increased methylation of CDKN2B (p15), OSCP1, PGRA, PGRB, and TFAP2E genes was described during CML progression [39]. Another extensive study defined 33 highly methylation-affected regions with hypermethylation of ABL1, WT1, ZNF577, and hypomethylation of G6B and TRIM15 [40]. DDX43 represents a frequently overexpressed gene in hematological malignancies. Epigenetic regulation of DDX43 by promoter methylation and a negative correlation between hypomethylation and higher expression of DDX43 was observed in CML patients. Moreover, the frequency of DDX43 hypomethylation increased in CP, AP, and BP by 23.4%, 25.0%, and 33.3%, respectively [41].
Promoter hypermethylation is mostly associated with TSGs involved in crucial cell functions, including the regulation of differentiation, proliferation, apoptosis, cell cycle, and growth. Similar to AML, HOXA4 and HOXA5 were hypermethylated in CML patients, and the presence of promoter methylation correlated with resistance to imatinib, belonging to a group of TKIs. Patients with HOXA4 and HOXA5 methylation levels higher than 63% showed 3.78- and 3.95-times-higher risk for imatinib resistance, respectively [42]. Imatinib resistance was also recorded in the case of higher methylation of OSCP1 and NPM2 genes [39]. Aberrant methylation of TSGs, including PLCD1, DLX4, DDIT3, PU.1, DAPK1, BIM, and GPX3, could represent potential prognostic or therapeutic targets in CML [43][44][45][46][47][48][49]. Accordingly, the downregulation of SHP-1 and relevant protein expression was associated with the presence of promoter methylation in advanced CML patients. SHP-1, a protein tyrosine phosphatase, is expressed mainly in HSC and plays a critical role in the regulation of JAK/STAT and MYC, AKT, and MAPK pathways. Alterations in SHP-1 methylation status could lead to the deregulation of included pathways and blastic transformation in CML patients [50].

2.3. Aberrant Methylation in ALL

ALL represents hematological cancer of immature T or B cells. T-ALL and B-ALL represent approximately 15–20% and 85% of all cases, respectively [51]. A genome-wide methylation analysis, based on nine genes with the identified CpG methylator phenotype, was capable of predicting a poor outcome subgroup of adult T-ALL. Patients with low methylation levels reported shorter OS and a higher risk of death in univariate and multivariate analyses. According to the results, the lowest methylation levels in patients were significantly associated with gender, younger age, and a higher count of white blood cells [52]. Similar to AML, CDKN2B (p15) presented decreased expression in most T-ALL cases, originating from deletion and promoter hypermethylation. CDKN2B hypermethylation frequently occurred together with mutations in DNMT3A and NRAS genes. In addition to the association with an older age of onset, the results showed a relatively early presence of T-cell precursors of ALL, causing the quick arrest of T-cell differentiation [53]. DLX3, belonging to the DLX gene family with a wide range of functions during hematopoiesis, could be active in the resistance to apoptosis. In pediatric B-cell ALL, Campo Dell Orto et al. observed aberrant methylation of DLX3 with reduced gene expression in patients with MLL-AF4 fusion, while no methylation was found in the subgroup with the TEL-AML1 fusion protein. The results suggested a potential role of DLX3 methylation in B-cell acute leukemias [54]. Some epigenetic promoter alterations can be lineage-specific. Higher methylation and methylation-mediated downregulation of RUNDC3B expression are typical for lymphoid but not myeloid malignancies [55]. RUNDC3B participates in the MAPK cascade in the role of Rap2-MAPK signaling mediator. Silencing by promoter methylation could disrupt the MAPK signaling pathway and promote leukemogenesis of lymphoid cells [55].
Several specific methylation profiles were found to be diagnostic, prognostic, or therapeutic markers for ALL. Chatterton et al. monitored the methylation of FOXE3 and TLX3 genes, showing their ability to discriminate between cancerous and healthy bone marrow samples with high specificity and sensitivity, which indicates their potential as diagnostic markers. Furthermore, TLX3 methylation correlated with minimal residual disease (MRD) in pediatric ALL patients [56]. In B-ALL and T-ALL-, RASSF6 and RASSF10 genes were frequently methylated and associated with the MRD in peripheral blood samples of adult ALL patients. In addition, the hypermethylation of RASSF6 is significantly associated with shorter OS in precursor B-ALL patients [57][58]. In a series of further studies, Roman-Gomez et al. described the role of promoter hypermethylation in a prognostic manner while observing the association of higher methylation of p21, WNT5A, Dkk-3, and NES1 genes with shorter disease-free survival (DFS) and OS [59][60][61][62]. Higher methylation of the PCDH17 gene was frequently observed in both B-ALL and T-ALL with a relationship with lower OS and increased risk for relapse and death [63][64].
Importantly, the specific gene methylation profiles can represent a predictive therapeutic marker because aberrant methylation can be responsible for chemotherapeutic resistance. TWIST2 hypermethylation and its inactivation were observed in more than 50% of ALL patients and 91% of samples from relapsed patients. In vitro experiments showed that the re-expression of TWIST2 increased apoptosis and sensitivity to chemotherapeutics [65]. A comparison of chemo-resistant and sensitive B-ALL pediatric patients detected higher levels of methylation in ADAMTSL5 (93% vs. 38%) and CDH11 (79% vs. 40%) in chemo-resistant vs. chemo-sensitive patients, respectively [66]. On the other hand, hypomethylation of the ASNS gene in T-ALL childhood patients was associated with poor outcomes and resistance to asparaginase, which is a high-dose drug involved in T-ALL therapy [67].

2.4. Aberrant Methylation in CLL

CLL belongs to the most common leukemias in the adult population, characterized by the clonal expansion of malignant B cells [68]. Similar to some other hematological malignancies, global hypomethylation is a characteristic sign of CLL. However, aberrantly methylated regions were characterized for this diagnosis in previous years [69]. In a genome-wide methylation study, Pei et al. identified approximately 1764 known genes with different methylation at 5’ regulatory regions. Among them, the results showed the presence of aberrant methylation in all four HOX gene clusters [11]. Previously, several studies found higher promoter methylation of genes, including TWIST2, DAPK1, SLIT2, or ZAP70 [70][71][72][73]. Increased expression of ZAP70 predicts poor outcomes for CLL patients, and CpG sites important for the regulation of transcription were identified in the 5’ regulatory region. According to these findings, a decreased methylation level in this specific CpG dinucleotide is a predictive biomarker for poor prognosis [73]. The WNT signaling pathway is generally involved in carcinogenesis and leukemogenesis. The constantly activated WNT pathway associated with the detection of hypermethylation of the seven WNT antagonist genes WIF1, DKK3, APC, SFRP1, SFRP2, SFRP4, and SFRP5 was observed in the peripheral blood of CLL patients. However, no association between methylation and clinical parameters was confirmed [74][75].
The hormone peptide Endothelin-1 (ET-1) plays a role in various cell functions, including proliferation. Microenvironment stimuli activated downstream receptors, leading to increased ET-1 expression in CLL. The unmethylated ET-1 gene was observed in healthy donors, while CLL patient samples exhibited 32% unmethylated and 68% methylated profiles. As shown, high methylation of the first ET-1 intron decreased its expression, suggesting the importance of epigenetic regulation [76]. The same authors detected that low methylation levels of neoangiogenic factor ANGPT2 correlated with increased expression and are associated with shorter OS and poor prognosis in CLL patients [77]. The prognostic potential of aberrant methylation in CLL was also observed in other genes, including PAX9, DUSP22, RPRM, SASH1, and CRY1 [78][79][80]. In the low-risk CLL subgroup, the hypermethylated CRY1 promoter inactivated its expression and was associated with better outcomes [80]. For CLL diagnosis, the most differentially methylated gene SHANK1 positively correlated with the absolute lymphocyte count. Increased SHANK1 methylation was found in samples during the pre-CLL diagnosis period, suggesting that epigenetic modification of SHANK1 occurred early in CLL carcinogenesis [81]. In comparison with SHANK1, the NFATC1 gene belongs to the most hypomethylated genes identified in CLL. A decreased methylation level is strongly associated with NFATC1 upregulation, resulting in the deregulation of target gene expression. Inactivation of NFAT regulator calcineurin by ibrutinib increased apoptosis in leukemic cells. Thus, NFACT1 might represent a potential therapeutic target in CLL diagnosis [82].
Two subgroups of CLL patients are classified according to the presence of somatic mutations in immunoglobulin (Ig) genes. The Ig heavy chain variable region (IGHV) with high mutational prevalence (IGHV-M) correlated with a more favorable prognosis compared to IGHV unmutated (IGHV-UM) CLL patients [83]. In the IGHV-UM subgroup, VHL and ABI3, acting as TSGs, were preferentially methylated and correlated with decreasing expression [84]. In contrast, the expression of WNT5A distinguished patients with worse outcomes in the IGHV-M subgroup. Reduced WNT5A expression through hypermethylation preferentially in three CpG dinucleotides within the regulatory region correlated with good prognoses [85].

2.5. Aberrant Methylation in Malignant Lymphomas

Malignant lymphomas (MLs) represent a heterogeneous group of hematologic malignancies in primary or secondary lymphatic organs arising from various types of B and T lymphocytes or NK cells. Generally, MLs cover classical Hodgkin lymphomas (HL) and diverse groups of non-Hodgkin lymphomas (NHL) [86]. Pathogenesis of HL is presumably associated with family anamnesis or infection with Epstein–Barr virus (EBV). According to the previous findings in HL, DNA methylation led to the silencing of RASSF1A, p16INK4a, p18INK4c, p15INK4b, SYK, BOB.1/OBF.1, and CD79B [87][88][89]. Recently, differences in DNA methylation signatures were detected in a study with monozygotic triplets with HL. Two of the triplets with HL shared DNA methylation changes in naive B-cells and marginal zone-like B-cells compared to a healthy non-HL-triplet. Hypermethylation of one region within chromosome 18 in naive B-cells was found exclusively in HL triplets [90].
Bethge et al. identified 233 downregulated genes in a cohort of B-cell NHL patients. From the analyzed gene panel, DSP, FZD8, KCNH2, and PPP1R14A exhibited promoter methylation in 28%, 67%, 22%, and 78%, respectively. In addition, the highest methylation level after treatment with demethylating agents was detected in LRP12 and CDH1 genes, presenting 94% and 92%, respectively [91][92]. Another study evaluated eight genes associated with lymphoma pathogenesis and found decreased SIRT1 and increased KLF4, DAPK1, and SPG20 gene methylation levels. In vitro analysis revealed that DNMT1 did not affect hypermethylation maintenance of KLF4, DAPK1, and SPG20 genes [93][94].
Most DNA methylation studies have been performed in NHL, specifically in diffuse large B-cell lymphoma (DLBCL) (Figure 2). The results from the genome-wide methylation study reported approximately 200 differentially methylated genes in DLBCL patients with the aberrant methylation of p16/CDKN2A, p21/CDKN1A, and p27/CDKN1B [95]. However, only 37% of DLBCL patients had p16 methylation higher than 5%. According to the results, patients younger than 65 years manifested better progression-free survival (PFS) when the p16 methylation level reached more than 25% [96]. Aberrant methylation of another from CDK inhibitors, p57/KIP2, suggested that epigenetic modification of p57 could be established as a biomarker for MRD in DLBCL [97]. Shawky et al. analyzed the panel of 20 TSGs for promoter hypermethylation and correlation with clinical characteristics and patient outcomes in the DLBCL group. The methylation of several studied genes associated with survival and chemoresistance, specifically RUNX3, DAPK1, and MT16, represent prognostic factors for DFS. Moreover, hypermethylation of RUNX3 and CDH1 was shown to be an independent prognostic factor for OS [98].
Figure 2. Methylation-affected genes associated with lymphomas. Aberrant DNA methylation (a red circle) regulates gene expression in Hodgkin, Non-Hodgkin, and Diffuse large B-cell lymphomas. Abnormally methylated genes might be used as potential biomarkers for therapeutic decisions and as predictive markers for patient outcomes.
Promoter methylation analysis of genes coding cadherins and protocadherins uncovered the association of CDH23 and PCDH10 hypermethylation and downregulated expression with worse outcomes in DLBCL patients. As noted, methylation of these genes could serve as a risk marker or a potential therapeutic target [99][100]. Several methylation studies investigated the DAPK1 gene showing higher methylation significantly associated with lower OS, disease-specific survival, and 5- year survival in the DLBCL patient cohort [101][102]. In addition, a prognostic and predictive potential of increased DAPK1 methylation in plasma samples was revealed in DLBCL. Patients with decreased methylation levels survived longer than patients with unchanged or regained DAPK1 methylation [103]. Specific promoter methylation with prognostic significance for DLBCL patients was discovered in several other genes, including SLIT2, DUSP4, and MGMT [104][105][106]. Importantly, Clozel et al. documented the link between aberrant DNA methylation and resistance to chemotherapeutics. Methylation analysis in chemoresistant DLBCL patients found nine hypermethylated genes. Among them, SMAD1 was a critical player. In a clinical trial on DLBCL patients, treatment with azacitidine followed by chemoimmunotherapy showed demethylation of SMAD1 and increased chemosensitivity [107].

In conclusion, DNA methylation is one of the main epigenetic mechanisms, besides known genetic alterations, that play a role in cancer initiation and progression. In the future, DNA methylation-based stratification of hematologic patients might lead to more personalized treatment with better outcomes. The reversibility of changes in DNA methylation landscapes enables broad clinical implications. However, adverse events associated with indiscriminate global hypomethylation with DNA methylation inhibitors are a matter of concern, and further investigations are highly warranted. According to the findings, DNA methylation processes in hematologic malignancies are usually associated with other mechanisms of epigenetic regulations, including histone modifications and miRNA regulation. Thus, evaluating the role of epigenetic modifications in a more complex matter would be beneficial for blood cancer patients.

 

References

  1. Goldberg, A.D.; Allis, C.D.; Bernstein, E. Epigenetics: A landscape takes shape. Cell 2007, 128, 635–638.
  2. Zeng, Y.; Chen, T. DNA Methylation Reprogramming during Mammalian Development. Genes 2019, 10, 257.
  3. Hanahan, D. Hallmarks of Cancer: New Dimensions. Cancer Discov. 2022, 12, 31–46.
  4. Weisenberger, D.J.; Lakshminarasimhan, R.; Liang, G. The Role of DNA Methylation and DNA Methyltransferases in Cancer. Adv. Exp. Med. Biol. 2022, 1389, 317–348.
  5. Dan, J.; Chen, T. Genetic Studies on Mammalian DNA Methyltransferases. Adv. Exp. Med. Biol. 2022, 1389, 111–136.
  6. Cypris, O.; Frobel, J.; Rai, S.; Franzen, J.; Sontag, S.; Goetzke, R.; de Toledo, M.A.S.; Zenke, M.; Wagner, W. Tracking of epigenetic changes during hematopoietic differentiation of induced pluripotent stem cells. Clin. Epigenet. 2019, 11, 19.
  7. Farlik, M.; Halbritter, F.; Muller, F.; Choudry, F.A.; Ebert, P.; Klughammer, J.; Farrow, S.; Santoro, A.; Ciaurro, V.; Mathur, A.; et al. DNA Methylation Dynamics of Human Hematopoietic Stem Cell Differentiation. Cell Stem Cell 2016, 19, 808–822.
  8. Guillamot, M.; Cimmino, L.; Aifantis, I. The Impact of DNA Methylation in Hematopoietic Malignancies. Trends Cancer 2016, 2, 70–83.
  9. Gao, H.; He, X.; Li, Q.; Wang, Y.; Tian, Y.; Chen, X.; Wang, J.; Guo, Y.; Wang, W.; Li, X. Genome-wide DNA methylome analysis reveals methylation subtypes with different clinical outcomes for acute myeloid leukemia patients. Cancer Med. 2020, 9, 6296–6305.
  10. Heller, G.; Topakian, T.; Altenberger, C.; Cerny-Reiterer, S.; Herndlhofer, S.; Ziegler, B.; Datlinger, P.; Byrgazov, K.; Bock, C.; Mannhalter, C.; et al. Next-generation sequencing identifies major DNA methylation changes during progression of Ph+ chronic myeloid leukemia. Leukemia 2016, 30, 1861–1868.
  11. Pei, L.; Choi, J.H.; Liu, J.; Lee, E.J.; McCarthy, B.; Wilson, J.M.; Speir, E.; Awan, F.; Tae, H.; Arthur, G.; et al. Genome-wide DNA methylation analysis reveals novel epigenetic changes in chronic lymphocytic leukemia. Epigenetics 2012, 7, 567–578.
  12. Hetzel, S.; Mattei, A.L.; Kretzmer, H.; Qu, C.; Chen, X.; Fan, Y.; Wu, G.; Roberts, K.G.; Luger, S.; Litzow, M.; et al. Acute lymphoblastic leukemia displays a distinct highly methylated genome. Nat. Cancer 2022, 3, 768–782.
  13. Haider, Z.; Landfors, M.; Golovleva, I.; Erlanson, M.; Schmiegelow, K.; Flaegstad, T.; Kanerva, J.; Noren-Nystrom, U.; Hultdin, M.; Degerman, S. DNA methylation and copy number variation profiling of T-cell lymphoblastic leukemia and lymphoma. Blood Cancer J. 2020, 10, 45.
  14. Liu, P.; Jiang, W.; Zhao, J.; Zhang, H. Integrated analysis of genome-wide gene expression and DNA methylation microarray of diffuse large B-cell lymphoma with TET mutations. Mol. Med. Rep. 2017, 16, 3777–3782.
  15. Roux, B.; Picou, F.; Debeissat, C.; Koubi, M.; Gallay, N.; Hirsch, P.; Ravalet, N.; Bene, M.C.; Maigre, M.; Hunault, M.; et al. Aberrant DNA methylation impacts HOX genes expression in bone marrow mesenchymal stromal cells of myelodysplastic syndromes and de novo acute myeloid leukemia. Cancer Gene Ther. 2022, 29, 1263–1275.
  16. Sampath, S.; Misra, P.; Yadav, S.K.; Sharma, S.; Somasundaram, V. A study on DNA methylation status in promoter region of p15 gene in patients of acute myeloid leukemia and myelodysplastic syndrome. Med. J. Armed Forces India 2021, 77, 337–342.
  17. Fu, H.Y.; Wu, D.S.; Zhou, H.R.; Shen, J.Z. CpG island methylator phenotype and its relationship with prognosis in adult acute leukemia patients. Hematology 2014, 19, 329–337.
  18. Kurtovic, N.K.; Krajnovic, M.; Bogdanovic, A.; Suvajdzic, N.; Jovanovic, J.; Dimitrijevic, B.; Colovic, M.; Krtolica, K. Concomitant aberrant methylation of p15 and MGMT genes in acute myeloid leukemia: Association with a particular immunophenotype of blast cells. Med. Oncol. 2012, 29, 3547–3556.
  19. Hiller, J.K.; Schmoor, C.; Gaidzik, V.I.; Schmidt-Salzmann, C.; Yalcin, A.; Abdelkarim, M.; Blagitko-Dorfs, N.; Dohner, K.; Bullinger, L.; Duyster, J.; et al. Evaluating the impact of genetic and epigenetic aberrations on survival and response in acute myeloid leukemia patients receiving epigenetic therapy. Ann. Hematol. 2017, 96, 559–565.
  20. Lian, X.Y.; Ma, J.C.; Zhou, J.D.; Zhang, T.J.; Wu, D.H.; Deng, Z.Q.; Zhang, Z.H.; Li, X.X.; He, P.F.; Yan, Y.; et al. Hypermethylation of ITGBL1 is associated with poor prognosis in acute myeloid leukemia. J. Cell. Physiol. 2019, 234, 9438–9446.
  21. Zhang, Z.H.; Zhang, W.; Zhou, J.D.; Zhang, T.J.; Ma, J.C.; Xu, Z.J.; Lian, X.Y.; Wu, D.H.; Wen, X.M.; Deng, Z.Q.; et al. Decreased SCIN expression, associated with promoter methylation, is a valuable predictor for prognosis in acute myeloid leukemia. Mol. Carcinog. 2018, 57, 735–744.
  22. Zhang, T.J.; Xu, Z.J.; Gu, Y.; Wen, X.M.; Ma, J.C.; Zhang, W.; Deng, Z.Q.; Leng, J.Y.; Qian, J.; Lin, J.; et al. Identification and validation of prognosis-related DLX5 methylation as an epigenetic driver in myeloid neoplasms. Clin. Transl. Med. 2020, 10, e29.
  23. Park, S.; So, M.K.; Huh, J. Methylation of DNA Repair Genes as a Prognostic Biomarker in AML of a TCGA-LAML Cohort. Clin. Lab. 2022, 68, 7.
  24. Chaubey, R.; Sazawal, S.; Mahapatra, M.; Chhikara, S.; Saxena, R. Prognostic relevance of aberrant SOCS-1 gene promoter methylation in myelodysplastic syndromes patients. Int. J. Lab. Hematol. 2015, 37, 265–271.
  25. He, P.F.; Xu, Z.J.; Zhou, J.D.; Li, X.X.; Zhang, W.; Wu, D.H.; Zhang, Z.H.; Lian, X.Y.; Yao, X.Y.; Deng, Z.Q.; et al. Methylation-associated DOK1 and DOK2 down-regulation: Potential biomarkers for predicting adverse prognosis in acute myeloid leukemia. J. Cell. Physiol. 2018, 233, 6604–6614.
  26. Sun, G.K.; Tang, L.J.; Zhou, J.D.; Xu, Z.J.; Yang, L.; Yuan, Q.; Ma, J.C.; Liu, X.H.; Lin, J.; Qian, J.; et al. DOK6 promoter methylation serves as a potential biomarker affecting prognosis in de novo acute myeloid leukemia. Cancer Med. 2019, 8, 6393–6402.
  27. Bozic, T.; Lin, Q.; Frobel, J.; Wilop, S.; Hoffmann, M.; Muller-Tidow, C.; Brummendorf, T.H.; Jost, E.; Wagner, W. DNA-methylation in C1R is a prognostic biomarker for acute myeloid leukemia. Clin. Epigenet. 2015, 7, 116.
  28. Sestakova, S.; Cerovska, E.; Salek, C.; Kundrat, D.; Jeziskova, I.; Folta, A.; Mayer, J.; Racil, Z.; Cetkovsky, P.; Remesova, H. A validation study of potential prognostic DNA methylation biomarkers in patients with acute myeloid leukemia using a custom DNA methylation sequencing panel. Clin. Epigenet. 2022, 14, 22.
  29. Zhou, J.D.; Yao, D.M.; Zhang, Y.Y.; Ma, J.C.; Wen, X.M.; Yang, J.; Guo, H.; Chen, Q.; Lin, J.; Qian, J. GPX3 hypermethylation serves as an independent prognostic biomarker in non-M3 acute myeloid leukemia. Am. J. Cancer Res. 2015, 5, 1786–1794.
  30. Zhou, J.D.; Zhang, T.J.; Wang, Y.X.; Yang, D.Q.; Yang, L.; Ma, J.C.; Wen, X.M.; Yang, J.; Lin, J.; Qian, J. DLX4 hypermethylation is a prognostically adverse indicator in de novo acute myeloid leukemia. Tumour Biol. 2016, 37, 8951–8960.
  31. Li, J.; Jin, W.; Tan, Y.; Wang, B.; Wang, X.; Zhao, M.; Wang, K. Distinct gene expression pattern of RUNX1 mutations coordinated by target repression and promoter hypermethylation in acute myeloid leukemia. Front. Med. 2022, 16, 627–636.
  32. Man, C.H.; Fung, T.K.; Wan, H.; Cher, C.Y.; Fan, A.; Ng, N.; Ho, C.; Wan, T.S.; Tanaka, T.; So, C.W.; et al. Suppression of SOX7 by DNA methylation and its tumor suppressor function in acute myeloid leukemia. Blood 2015, 125, 3928–3936.
  33. Gu, Y.; Zhou, J.D.; Xu, Z.J.; Zhang, T.J.; Wen, X.M.; Ma, J.C.; Ji, R.B.; Yuan, Q.; Zhang, W.; Chen, Q.; et al. Promoter methylation of the candidate tumor suppressor gene TCF21 in myelodysplastic syndrome and acute myeloid leukemia. Am. J. Transl. Res. 2019, 11, 3450–3460.
  34. Chen, X.X.; Lin, J.; Qian, J.; Qian, W.; Yang, J.; Ma, J.C.; Deng, Z.Q.; An, C.; Tang, C.Y.; Qian, Z.; et al. Methylation of CTNNA1 promoter: Frequent but not an adverse prognostic factor in acute myeloid leukemia. Leuk. Res. 2014, 38, 613–618.
  35. Chen, W.W.; Liu, D.B.; Xiao, H.X.; Zhou, L.J.; Qu, J. Identification of differentially expressed genes induced by aberrant methylation in acute myeloid leukemia using integrated bioinformatics analyses. Oncol. Lett. 2022, 24, 383.
  36. Lin, J.; Chen, Q.; Yang, J.; Qian, J.; Deng, Z.Q.; Qian, W.; Chen, X.X.; Ma, J.C.; Xiong, D.S.; Ma, Y.J.; et al. DDX43 promoter is frequently hypomethylated and may predict a favorable outcome in acute myeloid leukemia. Leuk. Res. 2014, 38, 601–607.
  37. Qu, X.; Othus, M.; Davison, J.; Wu, Y.; Yan, L.; Meshinchi, S.; Ostronoff, F.; Estey, E.H.; Radich, J.P.; Erba, H.P.; et al. Prognostic methylation markers for overall survival in cytogenetically normal patients with acute myeloid leukemia treated on SWOG trials. Cancer 2017, 123, 2472–2481.
  38. Minciacchi, V.R.; Kumar, R.; Krause, D.S. Chronic Myeloid Leukemia: A Model Disease of the Past, Present and Future. Cells 2021, 10, 117.
  39. Jelinek, J.; Gharibyan, V.; Estecio, M.R.; Kondo, K.; He, R.; Chung, W.; Lu, Y.; Zhang, N.; Liang, S.; Kantarjian, H.M.; et al. Aberrant DNA methylation is associated with disease progression, resistance to imatinib and shortened survival in chronic myelogenous leukemia. PLoS ONE 2011, 6, e22110.
  40. Maupetit-Mehouas, S.; Court, F.; Bourgne, C.; Guerci-Bresler, A.; Cony-Makhoul, P.; Johnson, H.; Etienne, G.; Rousselot, P.; Guyotat, D.; Janel, A.; et al. DNA methylation profiling reveals a pathological signature that contributes to transcriptional defects of CD34+ CD15− cells in early chronic-phase chronic myeloid leukemia. Mol. Oncol. 2018, 12, 814–829.
  41. Chen, Q.; Lin, J.; Qian, J.; Deng, Z.Q.; Qian, W.; Yang, J.; Li, Y.; Chen, X.X.; Ma, Y.J.; Ma, J.C.; et al. The methylation status of the DDX43 promoter in Chinese patients with chronic myeloid leukemia. Genet. Test. Mol. Biomarkers 2013, 17, 508–511.
  42. Elias, M.H.; Azlan, H.; Sulong, S.; Baba, A.A.; Ankathil, R. Aberrant DNA methylation at HOXA4 and HOXA5 genes are associated with resistance to imatinib mesylate among chronic myeloid leukemia patients. Cancer Rep. 2018, 1, e1111.
  43. Song, J.J.; Liu, Q.; Li, Y.; Yang, Z.S.; Yang, L.; Xiang, T.X.; Ren, G.S.; Chen, J.B. Epigenetic inactivation of PLCD1 in chronic myeloid leukemia. Int. J. Mol. Med. 2012, 30, 179–184.
  44. Zhou, J.D.; Wang, Y.X.; Zhang, T.J.; Yang, D.Q.; Yao, D.M.; Guo, H.; Yang, L.; Ma, J.C.; Wen, X.M.; Yang, J.; et al. Epigenetic inactivation of DLX4 is associated with disease progression in chronic myeloid leukemia. Biochem. Biophys. Res. Commun. 2015, 463, 1250–1256.
  45. Wang, Y.L.; Qian, J.; Lin, J.; Yao, D.M.; Qian, Z.; Zhu, Z.H.; Li, J.Y. Methylation status of DDIT3 gene in chronic myeloid leukemia. J. Exp. Clin. Cancer Res. 2010, 29, 54.
  46. Yang, H.; Liang, H.; Yan, J.S.; Tao, R.; Hao, S.G.; Ma, L.Y. Down-regulation of hematopoiesis master regulator PU.1 via aberrant methylation in chronic myeloid leukemia. Int. J. Hematol. 2012, 96, 65–73.
  47. Qian, J.; Wang, Y.L.; Lin, J.; Yao, D.M.; Xu, W.R.; Wu, C.Y. Aberrant methylation of the death-associated protein kinase 1 (DAPK1) CpG island in chronic myeloid leukemia. Eur. J. Haematol. 2009, 82, 119–123.
  48. San Jose-Eneriz, E.; Agirre, X.; Jimenez-Velasco, A.; Cordeu, L.; Martin, V.; Arqueros, V.; Garate, L.; Fresquet, V.; Cervantes, F.; Martinez-Climent, J.A.; et al. Epigenetic down-regulation of BIM expression is associated with reduced optimal responses to imatinib treatment in chronic myeloid leukaemia. Eur. J. Cancer 2009, 45, 1877–1889.
  49. Yao, D.M.; Zhou, J.D.; Zhang, Y.Y.; Yang, L.; Wen, X.M.; Yang, J.; Guo, H.; Chen, Q.; Lin, J.; Qian, J. GPX3 promoter is methylated in chronic myeloid leukemia. Int. J. Clin. Exp. Pathol. 2015, 8, 6450–6457.
  50. Li, Y.; Yang, L.; Pan, Y.; Yang, J.; Shang, Y.; Luo, J. Methylation and decreased expression of SHP-1 are related to disease progression in chronic myelogenous leukemia. Oncol. Rep. 2014, 31, 2438–2446.
  51. Wenzinger, C.; Williams, E.; Gru, A.A. Updates in the Pathology of Precursor Lymphoid Neoplasms in the Revised Fourth Edition of the WHO Classification of Tumors of Hematopoietic and Lymphoid Tissues. Curr. Hematol. Malig. Rep. 2018, 13, 275–288.
  52. Touzart, A.; Boissel, N.; Belhocine, M.; Smith, C.; Graux, C.; Latiri, M.; Lhermitte, L.; Mathieu, E.L.; Huguet, F.; Lamant, L.; et al. Low level CpG island promoter methylation predicts a poor outcome in adult T-cell acute lymphoblastic leukemia. Haematologica 2020, 105, 1575–1581.
  53. Jang, W.; Park, J.; Kwon, A.; Choi, H.; Kim, J.; Lee, G.D.; Han, E.; Jekarl, D.W.; Chae, H.; Han, K.; et al. CDKN2B downregulation and other genetic characteristics in T-acute lymphoblastic leukemia. Exp. Mol. Med. 2019, 51, 1–15.
  54. Dell’Orto, M.C.; Banelli, B.; Giarin, E.; Accordi, B.; Trentin, L.; Romani, M.; Te Kronnie, G.; Basso, G. Down-regulation of DLX3 expression in MLL-AF4 childhood lymphoblastic leukemias is mediated by promoter region hypermethylation. Oncol. Rep. 2007, 18, 417–423.
  55. Burmeister, D.W.; Smith, E.H.; Cristel, R.T.; McKay, S.D.; Shi, H.; Arthur, G.L.; Davis, J.W.; Taylor, K.H. The expression of RUNDC3B is associated with promoter methylation in lymphoid malignancies. Hematol. Oncol. 2017, 35, 25–33.
  56. Chatterton, Z.; Burke, D.; Emslie, K.R.; Craig, J.M.; Ng, J.; Ashley, D.M.; Mechinaud, F.; Saffery, R.; Wong, N.C. Validation of DNA methylation biomarkers for diagnosis of acute lymphoblastic leukemia. Clin. Chem. 2014, 60, 995–1003.
  57. Younesian, S.; Shahkarami, S.; Ghaffari, P.; Alizadeh, S.; Mehrasa, R.; Ghavamzadeh, A.; Ghaffari, S.H. DNA hypermethylation of tumor suppressor genes RASSF6 and RASSF10 as independent prognostic factors in adult acute lymphoblastic leukemia. Leuk. Res. 2017, 61, 33–38.
  58. Younesian, S.; Shahkarami, S.; Ghaffari, P.; Alizadeh, S.; Mehrasa, R.; Ghaffari, S.H. Residual methylation of tumor suppressor gene promoters, RASSF6 and RASSF10, as novel biomarkers for minimal residual disease detection in adult acute lymphoblastic leukemia. Ann. Hematol. 2019, 98, 2719–2727.
  59. Roman-Gomez, J.; Castillejo, J.A.; Jimenez, A.; Gonzalez, M.G.; Moreno, F.; Rodriguez, M.C.; Barrios, M.; Maldonado, J.; Torres, A. 5’ CpG island hypermethylation is associated with transcriptional silencing of the p21(CIP1/WAF1/SDI1) gene and confers poor prognosis in acute lymphoblastic leukemia. Blood 2002, 99, 2291–2296.
  60. Roman-Gomez, J.; Jimenez-Velasco, A.; Cordeu, L.; Vilas-Zornoza, A.; San Jose-Eneriz, E.; Garate, L.; Castillejo, J.A.; Martin, V.; Prosper, F.; Heiniger, A.; et al. WNT5A, a putative tumour suppressor of lymphoid malignancies, is inactivated by aberrant methylation in acute lymphoblastic leukaemia. Eur. J. Cancer 2007, 43, 2736–2746.
  61. Roman-Gomez, J.; Jimenez-Velasco, A.; Agirre, X.; Castillejo, J.A.; Navarro, G.; Barrios, M.; Andreu, E.J.; Prosper, F.; Heiniger, A.; Torres, A. Transcriptional silencing of the Dickkopfs-3 (Dkk-3) gene by CpG hypermethylation in acute lymphoblastic leukaemia. Br. J. Cancer 2004, 91, 707–713.
  62. Roman-Gomez, J.; Jimenez-Velasco, A.; Agirre, X.; Castillejo, J.A.; Barrios, M.; Andreu, E.J.; Prosper, F.; Heiniger, A.; Torres, A. The normal epithelial cell-specific 1 (NES1) gene, a candidate tumor suppressor gene on chromosome 19q13.3-4, is downregulated by hypermethylation in acute lymphoblastic leukemia. Leukemia 2004, 18, 362–365.
  63. Narayan, G.; Freddy, A.J.; Xie, D.; Liyanage, H.; Clark, L.; Kisselev, S.; Un Kang, J.; Nandula, S.V.; McGuinn, C.; Subramaniyam, S.; et al. Promoter methylation-mediated inactivation of PCDH10 in acute lymphoblastic leukemia contributes to chemotherapy resistance. Genes Chromosomes Cancer 2011, 50, 1043–1053.
  64. Uyen, T.N.; Sakashita, K.; Al-Kzayer, L.F.; Nakazawa, Y.; Kurata, T.; Koike, K. Aberrant methylation of protocadherin 17 and its prognostic value in pediatric acute lymphoblastic leukemia. Pediatr. Blood Cancer 2017, 64, e26259.
  65. Thathia, S.H.; Ferguson, S.; Gautrey, H.E.; van Otterdijk, S.D.; Hili, M.; Rand, V.; Moorman, A.V.; Meyer, S.; Brown, R.; Strathdee, G. Epigenetic inactivation of TWIST2 in acute lymphoblastic leukemia modulates proliferation, cell survival and chemosensitivity. Haematologica 2012, 97, 371–378.
  66. Abdullah, M.; Choo, C.W.; Alias, H.; Rahman, E.J.A.; Ibrahim, H.M.; Jamal, R.; Hussin, N.H. ADAMTSL5 and CDH11: Putative epigenetic markers for therapeutic resistance in acute lymphoblastic leukemia. Hematology 2017, 22, 386–391.
  67. Akahane, K.; Kimura, S.; Miyake, K.; Watanabe, A.; Kagami, K.; Yoshimura, K.; Shinohara, T.; Harama, D.; Kasai, S.; Goi, K.; et al. Association of allele-specific methylation of the ASNS gene with asparaginase sensitivity and prognosis in T-ALL. Blood Adv. 2022, 6, 212–224.
  68. Hallek, M.; Shanafelt, T.D.; Eichhorst, B. Chronic lymphocytic leukaemia. Lancet 2018, 391, 1524–1537.
  69. Cahill, N.; Rosenquist, R. Uncovering the DNA methylome in chronic lymphocytic leukemia. Epigenetics 2013, 8, 138–148.
  70. Raval, A.; Lucas, D.M.; Matkovic, J.J.; Bennett, K.L.; Liyanarachchi, S.; Young, D.C.; Rassenti, L.; Kipps, T.J.; Grever, M.R.; Byrd, J.C.; et al. TWIST2 demonstrates differential methylation in immunoglobulin variable heavy chain mutated and unmutated chronic lymphocytic leukemia. J. Clin. Oncol. 2005, 23, 3877–3885.
  71. Raval, A.; Tanner, S.M.; Byrd, J.C.; Angerman, E.B.; Perko, J.D.; Chen, S.S.; Hackanson, B.; Grever, M.R.; Lucas, D.M.; Matkovic, J.J.; et al. Downregulation of death-associated protein kinase 1 (DAPK1) in chronic lymphocytic leukemia. Cell 2007, 129, 879–890.
  72. Dunwell, T.L.; Dickinson, R.E.; Stankovic, T.; Dallol, A.; Weston, V.; Austen, B.; Catchpoole, D.; Maher, E.R.; Latif, F. Frequent epigenetic inactivation of the SLIT2 gene in chronic and acute lymphocytic leukemia. Epigenetics 2009, 4, 265–269.
  73. Claus, R.; Lucas, D.M.; Stilgenbauer, S.; Ruppert, A.S.; Yu, L.; Zucknick, M.; Mertens, D.; Buhler, A.; Oakes, C.C.; Larson, R.A.; et al. Quantitative DNA methylation analysis identifies a single CpG dinucleotide important for ZAP-70 expression and predictive of prognosis in chronic lymphocytic leukemia. J. Clin. Oncol. 2012, 30, 2483–2491.
  74. Chim, C.S.; Pang, R.; Liang, R. Epigenetic dysregulation of the Wnt signalling pathway in chronic lymphocytic leukaemia. J. Clin. Pathol. 2008, 61, 1214–1219.
  75. Bennett, L.B.; Taylor, K.H.; Arthur, G.L.; Rahmatpanah, F.B.; Hooshmand, S.I.; Caldwell, C.W. Epigenetic regulation of WNT signaling in chronic lymphocytic leukemia. Epigenomics 2010, 2, 53–70.
  76. Martinelli, S.; Maffei, R.; Fiorcari, S.; Quadrelli, C.; Zucchini, P.; Benatti, S.; Potenza, L.; Luppi, M.; Marasca, R. The expression of endothelin-1 in chronic lymphocytic leukemia is controlled by epigenetic mechanisms and extracellular stimuli. Leuk. Res. 2017, 54, 17–24.
  77. Martinelli, S.; Kanduri, M.; Maffei, R.; Fiorcari, S.; Bulgarelli, J.; Marasca, R.; Rosenquist, R. ANGPT2 promoter methylation is strongly associated with gene expression and prognosis in chronic lymphocytic leukemia. Epigenetics 2013, 8, 720–729.
  78. Rani, L.; Mathur, N.; Gupta, R.; Gogia, A.; Kaur, G.; Dhanjal, J.K.; Sundar, D.; Kumar, L.; Sharma, A. Genome-wide DNA methylation profiling integrated with gene expression profiling identifies PAX9 as a novel prognostic marker in chronic lymphocytic leukemia. Clin. Epigenet. 2017, 9, 57.
  79. Pan, H.; Renaud, L.; Chaligne, R.; Bloehdorn, J.; Tausch, E.; Mertens, D.; Fink, A.M.; Fischer, K.; Zhang, C.; Betel, D.; et al. Discovery of Candidate DNA Methylation Cancer Driver Genes. Cancer Discov. 2021, 11, 2266–2281.
  80. Hanoun, M.; Eisele, L.; Suzuki, M.; Greally, J.M.; Huttmann, A.; Aydin, S.; Scholtysik, R.; Klein-Hitpass, L.; Duhrsen, U.; Durig, J. Epigenetic silencing of the circadian clock gene CRY1 is associated with an indolent clinical course in chronic lymphocytic leukemia. PLoS ONE 2012, 7, e34347.
  81. Loi, E.; Moi, L.; Fadda, A.; Satta, G.; Zucca, M.; Sanna, S.; Amini Nia, S.; Cabras, G.; Padoan, M.; Magnani, C.; et al. Methylation alteration of SHANK1 as a predictive, diagnostic and prognostic biomarker for chronic lymphocytic leukemia. Oncotarget 2019, 10, 4987–5002.
  82. Wolf, C.; Garding, A.; Filarsky, K.; Bahlo, J.; Robrecht, S.; Becker, N.; Zucknick, M.; Rouhi, A.; Weigel, A.; Claus, R.; et al. NFATC1 activation by DNA hypomethylation in chronic lymphocytic leukemia correlates with clinical staging and can be inhibited by ibrutinib. Int. J. Cancer 2018, 142, 322–333.
  83. Fabbri, G.; Dalla-Favera, R. The molecular pathogenesis of chronic lymphocytic leukaemia. Nat. Rev. Cancer 2016, 16, 145–162.
  84. Kanduri, M.; Cahill, N.; Goransson, H.; Enstrom, C.; Ryan, F.; Isaksson, A.; Rosenquist, R. Differential genome-wide array-based methylation profiles in prognostic subsets of chronic lymphocytic leukemia. Blood 2010, 115, 296–305.
  85. Poppova, L.; Pavlova, S.; Gonzalez, B.; Kotaskova, J.; Plevova, K.; Dumbovic, G.; Janovska, P.; Bystry, V.; Panovska, A.; Bezdekova, L.; et al. Memory B-cell like chronic lymphocytic leukaemia is associated with specific methylation profile of WNT5A promoter and undetectable expression of WNT5A gene. Epigenetics 2022, 17, 1628–1635.
  86. Rodriguez-Abreu, D.; Bordoni, A.; Zucca, E. Epidemiology of hematological malignancies. Ann. Oncol. 2007, 18 (Suppl. S1), i3–i8.
  87. Murray, P.G.; Qiu, G.H.; Fu, L.; Waites, E.R.; Srivastava, G.; Heys, D.; Agathanggelou, A.; Latif, F.; Grundy, R.G.; Mann, J.R.; et al. Frequent epigenetic inactivation of the RASSF1A tumor suppressor gene in Hodgkin’s lymphoma. Oncogene 2004, 23, 1326–1331.
  88. Garcia, M.J.; Martinez-Delgado, B.; Cebrian, A.; Martinez, A.; Benitez, J.; Rivas, C. Different incidence and pattern of p15INK4b and p16INK4a promoter region hypermethylation in Hodgkin’s and CD30-Positive non-Hodgkin’s lymphomas. Am. J. Pathol. 2002, 161, 1007–1013.
  89. Ushmorov, A.; Leithauser, F.; Sakk, O.; Weinhausel, A.; Popov, S.W.; Moller, P.; Wirth, T. Epigenetic processes play a major role in B-cell-specific gene silencing in classical Hodgkin lymphoma. Blood 2006, 107, 2493–2500.
  90. Xia, C.; Olsen, T.K.; Zirakzadeh, A.A.; Almamoun, R.; Sjoholm, L.K.; Dahlstrom, J.; Sjoberg, J.; Claesson, H.E.; Johnsen, J.I.; Winqvist, O.; et al. Hodgkin Lymphoma Monozygotic Triplets Reveal Divergences in DNA Methylation Signatures. Front. Oncol. 2020, 10, 598872.
  91. Bethge, N.; Honne, H.; Hilden, V.; Troen, G.; Eknaes, M.; Liestol, K.; Holte, H.; Delabie, J.; Smeland, E.B.; Lind, G.E. Identification of highly methylated genes across various types of B-cell non-hodgkin lymphoma. PLoS ONE 2013, 8, e79602.
  92. Bethge, N.; Honne, H.; Andresen, K.; Hilden, V.; Troen, G.; Liestol, K.; Holte, H.; Delabie, J.; Lind, G.E.; Smeland, E.B. A gene panel, including LRP12, is frequently hypermethylated in major types of B-cell lymphoma. PLoS ONE 2014, 9, e104249.
  93. Frazzi, R.; Zanetti, E.; Pistoni, M.; Tamagnini, I.; Valli, R.; Braglia, L.; Merli, F. Methylation changes of SIRT1, KLF4, DAPK1 and SPG20 in B-lymphocytes derived from follicular and diffuse large B-cell lymphoma. Leuk. Res. 2017, 57, 89–96.
  94. Frazzi, R.; Cusenza, V.Y.; Pistoni, M.; Canovi, L.; Cascione, L.; Bertoni, F.; Merli, F. KLF4, DAPK1 and SPG20 promoter methylation is not affected by DNMT1 silencing and hypomethylating drugs in lymphoma cells. Oncol. Rep. 2022, 47, 10.
  95. Chambwe, N.; Kormaksson, M.; Geng, H.; De, S.; Michor, F.; Johnson, N.A.; Morin, R.D.; Scott, D.W.; Godley, L.A.; Gascoyne, R.D.; et al. Variability in DNA methylation defines novel epigenetic subgroups of DLBCL associated with different clinical outcomes. Blood 2014, 123, 1699–1708.
  96. Zainuddin, N.; Kanduri, M.; Berglund, M.; Lindell, M.; Amini, R.M.; Roos, G.; Sundstrom, C.; Enblad, G.; Rosenquist, R. Quantitative evaluation of p16(INK4a) promoter methylation using pyrosequencing in de novo diffuse large B-cell lymphoma. Leuk. Res. 2011, 35, 438–443.
  97. Hagiwara, K.; Li, Y.; Kinoshita, T.; Kunishma, S.; Ohashi, H.; Hotta, T.; Nagai, H. Aberrant DNA methylation of the p57KIP2 gene is a sensitive biomarker for detecting minimal residual disease in diffuse large B cell lymphoma. Leuk. Res. 2010, 34, 50–54.
  98. Shawky, S.A.; El-Borai, M.H.; Khaled, H.M.; Guda, I.; Mohanad, M.; Abdellateif, M.S.; Zekri, A.N.; Bahanasy, A.A. The prognostic impact of hypermethylation for a panel of tumor suppressor genes and cell of origin subtype on diffuse large B-cell lymphoma. Mol. Biol. Rep. 2019, 46, 4063–4076.
  99. Cao, B.; Guo, X.; Huang, L.; Wang, B.; Wang, W.; Han, D.; Zhang, W.; Zhong, K. Methylation silencing CDH23 is a poor prognostic marker in diffuse large B-cell lymphoma. Aging 2021, 13, 17768–17788.
  100. Huang, W.; Xue, X.; Shan, L.; Qiu, T.; Guo, L.; Ying, J.; Lu, N. Clinical significance of PCDH10 promoter methylation in diffuse large B-cell lymphoma. BMC Cancer 2017, 17, 815.
  101. Kristensen, L.S.; Asmar, F.; Dimopoulos, K.; Nygaard, M.K.; Aslan, D.; Hansen, J.W.; Ralfkiaer, E.; Gronbaek, K. Hypermethylation of DAPK1 is an independent prognostic factor predicting survival in diffuse large B-cell lymphoma. Oncotarget 2014, 5, 9798–9810.
  102. Wang, H.; Zhou, L.Y.; Guan, Z.B.; Zeng, W.B.; Zhou, L.L.; Liu, Y.N.; Pan, X.Y. Prognostic significance of DAPK promoter methylation in lymphoma: A meta-analysis. PLoS ONE 2019, 14, e0210943.
  103. Kristensen, L.S.; Hansen, J.W.; Kristensen, S.S.; Tholstrup, D.; Harslof, L.B.; Pedersen, O.B.; De Nully Brown, P.; Gronbaek, K. Aberrant methylation of cell-free circulating DNA in plasma predicts poor outcome in diffuse large B cell lymphoma. Clin. Epigenet. 2016, 8, 95.
  104. Mohamed, G.; Talima, S.; Li, L.; Wei, W.; Rudzki, Z.; Allam, R.M.; Simmons, W.; Tao, Q.; Murray, P.G. Low Expression and Promoter Hypermethylation of the Tumour Suppressor SLIT2, are Associated with Adverse Patient Outcomes in Diffuse Large B Cell Lymphoma. Pathol. Oncol. Res. 2019, 25, 1223–1231.
  105. Schmid, C.A.; Robinson, M.D.; Scheifinger, N.A.; Muller, S.; Cogliatti, S.; Tzankov, A.; Muller, A. DUSP4 deficiency caused by promoter hypermethylation drives JNK signaling and tumor cell survival in diffuse large B cell lymphoma. J. Exp. Med. 2015, 212, 775–792.
  106. Lee, S.M.; Lee, E.J.; Ko, Y.H.; Lee, S.H.; Maeng, L.; Kim, K.M. Prognostic significance of O6-methylguanine DNA methyltransferase and p57 methylation in patients with diffuse large B-cell lymphomas. APMIS 2009, 117, 87–94.
  107. Clozel, T.; Yang, S.; Elstrom, R.L.; Tam, W.; Martin, P.; Kormaksson, M.; Banerjee, S.; Vasanthakumar, A.; Culjkovic, B.; Scott, D.W.; et al. Mechanism-based epigenetic chemosensitization therapy of diffuse large B-cell lymphoma. Cancer Discov. 2013, 3, 1002–1019.
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