Desirable techno-functional properties of packaging materials such as permeability and mechanical and thermal properties play a key role in maintaining quality by preventing off-flavour development, contamination, browning, and softening, thereby extending the shelf life of mushrooms. Regardless of the type of packaging system, they all play an important role in maintaining or improving the quality of mushrooms and extending their shelf life. Each packaging system has unique performance characteristics defining their strengths/benefits in terms of preserving edible mushrooms, safety, and circular economy impact.
1. Introduction
Agaricus bisporus,
Lentinus edodes,
Pleurotus ostreatus,
Flammulina velutipes, and
Pleurotus eryngii are common edible mushroom species found in mainstream foods
[1]. Because of its great flavor and nutritional value,
Agaricus bisporus, commonly referred to as “white button mushroom”, is the most widely grown and consumed edible mushroom in the world, making up 30% of all mushroom production
[2]. It is a rich source of nutrients including protein, amino acids, and dietary fiber
[3][4][5], antioxidants
[5][6], terpenoids
[7], lectins
[8], phenolic compounds
[5][9], polysaccharides
[5][10], and ergosterols
[11][12].
Edible mushroom production and trade are expanding globally, with a significant positive impact on human living standards
[13]. According to the Global Mushroom Market (2023–2030) research report, the compound annual growth rate (CAGR) of the mushroom market increased globally by 9.2% from USD 57.18 billion in 2022 to USD 62.44 billion in 2023. At a CAGR of 9.8%, the mushroom market is projected to reach USD 90.88 billion in 2027. Teagasc Fact sheet Horticulture reported that around 68,000 tonnes of
Agaricus bisporus are produced annually in Ireland, nearly all of which are exported to the UK, with 20% used to supply the domestic market
[14]. The fact sheet states that the mushroom industry contributes to the Irish economy with a production value of approximately EUR 130 million in 2022. However, exporting to continental Europe is not feasible due to the short shelf life of mushrooms, as they typically only last three days at ambient conditions and five to eight days in a cold storage system
[15], as well as to the narrow margins that the industry faces.
The main contributing factors to the short shelf life of mushrooms are their high moisture content and enzyme activity coupled with their lack of a cuticle
[16], which aggravates the respiratory and metabolic rates of the seeds’ tender tissues. Additionally, these factors make mushrooms more vulnerable to mechanical damage and microbial contamination, which can result in browning and a decline in quality
[17][18][19]. These effects involve physicochemical quality degradation, including cap opening, loss of essential phenolic compounds, proteins, and vitamins, water loss, cell membrane deterioration, loss of firmness, and increased microbial activity in the process of storage and transportation
[20][21], in turn leading to loss of nutritional value, flavor, market acceptability value, and shelf life. Recent findings have reported a wide range of preservation techniques for fresh mushrooms, including irradiation
[22], ultrasonication
[22], pulsed eclectic field treatment
[23][24],
[25][26], 1-methylcyclopropene treatment
[27], modified atmosphere packaging, active packaging, edible coatings and nanopackaging
[1][15][28][29][30][31], and biodegradable packaging made from materials such as dextran/chitosan
[32].
More specifically, edible coatings and biodegradable packaging are safe and environmentally friendly packaging systems; they are made from natural substances such as pectin, chitosan, or sodium alginate to maintain quality and extend the shelf life of mushrooms
[32][33][34][35][36], and are mostly composed of active ingredients with antibacterial and antioxidant activities
[37][38]. Studies have shown that the incorporation of essential oils, active ingredients, and nanoparticles in edible coatings and biodegradable film packaging can improve the techno-functional properties of the packaging materials
[39]. Furthermore, tyrosine inhibitors such as plant extracts, fungus and bacterial extracts, and synthetic and natural phenolic compounds are utilized to control mushroom browning
[40][41][42][43].
2. Mechanisms of Action and Preservation Effects
2.1. Changes in Quality of Fresh Mushrooms
Fresh mushrooms are flavorful, have a moisture content ranging from 81.8% to 94.8%, and are rich sources of nutrients such as carbohydrates (50%–65%), protein (19%–35%), fat (2%–6%), minerals, dietary fiber, phenolic compounds, and vitamins
[44]. A wide range of studies have shown change in quality of fresh mushrooms during storage, including texture
[45][46][47], color
[16][48][49][50], nutrients, and flavor
[17][45][47][51][52][53]. These quality changes in mushrooms are attributed to the cumulative effects of respiratory, energy, membrane lipids, and reactive oxygen species metabolic reactions due to changes in enzymatic activity and microbial activity in response to intrinsic factors and the atmosphere surrounding the product. For example, the transport of electrons in mitochondria cells results in excessive reactive oxygen species accumulation such as H
2O
2 and O
2− in mushroom
[54]. These processes can lead to oxidative damage to nutrients such as membrane lipids, nucleic acids, and proteins as well as enzyme activity inhibition, leakage of electrolytes, and increased electrical conductivity, ultimately causing tissue aging, nutritional quality loss, and reduced shelf life of mushrooms
[46][55].
Figure 1 provides an overview of changes in mushroom quality along with the mechanisms of action and preservation effects of five major packaging systems for the preservation of fresh edible mushrooms: edible coatings, modified atmosphere packaging, active packaging, biodegradable packaging, and nanopackaging. For instance, edible coatings and biodegradable packaging made with active ingredients can successfully delay or minimize browning reactions by inhibiting tyrosinase and polyphenol oxidase activities in fresh mushrooms
[56][57][58][59].
Figure 1. Mechanisms of action and preservation effects of active packaging, edible coating, biodegradable packaging, modified atmosphere packaging, and nanopackaging for the preservation of fresh edible mushrooms. CAT—catalase, H2O2—hydrogen peroxide, MDA—malondialdehyde, O2−—superoxide radical, ROS—reactive oxygen species, SOD—superoxide dismutase, * an increase, ** a decrease.
Nanopackaging minimizes tissue aging, electrical conductivity, and the accumulation of reactive oxygen species (ROS) by controlling the energy metabolism and enzymatic activity of mushrooms
[29]. MAP lowers the rate of respiratory metabolism, thereby reducing the loss of cell wall components and cell swelling
[60]. By regulating metabolic enzyme activity, oxygen concentration, and energy metabolism, active packaging can lessen membrane lipid metabolism and microbial growth, thereby preventing loss of nutrients and flavor from mushrooms
[30][61]. The mechanisms of action of packaging systems can be explained by enzymatic, (such as tyrosinase and polyphenol oxidase activity), antimicrobial, antioxidant, and respiration activity
[59][62][63]. The following sections highlight the mechanisms of action and preservation effects of edible coatings, MAP, active packaging, biodegradable packaging, and nanopackaging.
2.2. Edible Coatings, Essential Oils, and Tyrosinase Inhibitors
Edible coatings have moderate to excellent barrier, preservative, cosmetic, and aesthetic qualities, are biocompatible and environmentally friendly, and can usually be consumed with food
[17]. Edible coatings combined with active ingredients can supply bioactive compounds to enhance the quality of edible mushrooms or prolong their shelf life, in addition to superior qualities of high air permeability and moisture permeability
[64]. Chitosan, guar gum, sodium alginate, aloe vera, leek powder, pectin, carboxymethyl, and cellulose are commonly used as edible coating materials for edible mushrooms; they are based on natural biopolymers with essential oils, nanoparticles, and active ingredients such as cinnamon (
Table 1). For example, the mixture of chitosan with guar gum can significantly increase antimicrobial activity, reduce cell wall and membrane destructive symptoms, and maintain higher firmness, protein, and ascorbic acid while increasing total soluble solids and reducing sugars of
Lentinus edodes mushrooms
[65]. Edible coatings made from a cinnamon nanoemulsion active ingredient in polymeric matrixes of alginate and glycerol significantly decreased the respiration rate, PPO activity, pseudomonas count, and weight loss while increasing antioxidant activity and maintaining firmness, color, and total polyphenols of
Agaricus bisporus mushrooms
[66].
Table 1. Mechanism of actions and preservation effects of edible coatings of fresh edible mushrooms.
Edible Coating |
Applicable |
Mechanism of Actions a |
Preservation Effects b |
Reference |
Chitosan-guar gum |
Lentinus edodes |
Significantly reduced cell wall and membrane destructive symptoms Increased antimicrobial activity |
Maintained higher firmness, protein, and ascorbic acid Increased total soluble solids and reducing sugars |
[65] |
Alginate-glycerol-cinnamon nanoemulsions |
Agaricus bisporus |
Decreased respiration rate Reduced polyphenol oxidase activity Reduced Pseudomonas counts Increased antioxidant activity |
Decreased weight loss Maintained firmness Maintained colour and total polyphenols |
[66] |
Pectin-chitosan-sodium alginate- carboxymethyl cellulose- N-acetyl cysteine |
Agaricus bisporus |
Controlled lipid peroxidation Increased antioxidant activity |
Delayed weight loss and cap opening |
[67] |
Aloe vera-basil essential oil |
Agaricus bisporus |
Reduced polyphenol oxidase, respiration, and electrolyte leakage rate Increased phenylalanine ammonia-lyase and antioxidant activity |
Reduced weight loss and softening Increased total phenolic contents Delayed browning and colour change |
[68] |
Leek powder sunflower oil-guar gum |
Agaricus bisporus |
Reduced the rate of respiration |
Reduced weight loss Maintained colour |
[69] |
Alginate-nanoAg-Silver nitrate-sodium Borohydride-polyvinylpyrrolidone |
Lentinus edodes |
Reduced the rate of respiration and physiological activity |
Extended shelf life Reduced weight loss softening, browning, and microbial counts. Increased total soluble solids |
[70] |