Submitted Successfully!
To reward your contribution, here is a gift for you: A free trial for our video production service.
Thank you for your contribution! You can also upload a video entry or images related to this topic.
Version Summary Created by Modification Content Size Created at Operation
1 -- 1028 2023-12-07 05:17:52 |
2 format correct Meta information modification 1028 2023-12-07 13:12:34 |

Video Upload Options

Do you have a full video?

Confirm

Are you sure to Delete?
Cite
If you have any further questions, please contact Encyclopedia Editorial Office.
Miyazaki, T. Molecular Basis of Cardiometabolic Calpain Isozymes and Calpastatin. Encyclopedia. Available online: https://encyclopedia.pub/entry/52470 (accessed on 25 June 2024).
Miyazaki T. Molecular Basis of Cardiometabolic Calpain Isozymes and Calpastatin. Encyclopedia. Available at: https://encyclopedia.pub/entry/52470. Accessed June 25, 2024.
Miyazaki, Takuro. "Molecular Basis of Cardiometabolic Calpain Isozymes and Calpastatin" Encyclopedia, https://encyclopedia.pub/entry/52470 (accessed June 25, 2024).
Miyazaki, T. (2023, December 07). Molecular Basis of Cardiometabolic Calpain Isozymes and Calpastatin. In Encyclopedia. https://encyclopedia.pub/entry/52470
Miyazaki, Takuro. "Molecular Basis of Cardiometabolic Calpain Isozymes and Calpastatin." Encyclopedia. Web. 07 December, 2023.
Molecular Basis of Cardiometabolic Calpain Isozymes and Calpastatin
Edit

Calpain is defined as a member of the superfamily of cysteine proteases possessing the CysPC motif within the gene. Calpain-1 and -2, which are categorized as conventional isozymes, execute limited proteolysis in a calcium-dependent fashion. Accordingly, the calpain system participates in physiological and pathological phenomena, including cell migration, apoptosis, and synaptic plasticity.

calpastatin calpain-10 calpain-6 calpain-1 calpain-2 conventional isozyme

1. Introduction

Obesity, diabetes, hypertension, and dyslipidemia are acknowledged to intersect via systemic metabolism, constituting potent common risk factors. This cluster of maladies is denoted as the metabolic syndrome, with its pernicious cycle of metabolic aberrations culminating in cardiovascular fatality [1][2]. In terms of diabetes, complications encompass chronic kidney diseases, significantly augmenting the propensity for coronary events [3][4]. Furthermore, dyslipidemia, as well as hyperglycemia and obesity, reportedly gives rise to intricacies involving steatohepatitis [5][6]. In contemporary times, this disease area has persistently broadened as a cardiometabolic disorder, prompting numerous epidemiological and molecular biomedical inquiries aimed at its prevention and amelioration. As a result of earlier studies, it was evidenced that statins have remarkable efficacy in rectifying dyslipidemia, with concomitant vasoprotective attributes [7][8]. Incretins and sodium glucose cotransporter 2 (sGLUT2) inhibitors are also ubiquitously employed in the realm of diabetes management, the former manifesting cardioprotective benefits as well as hepatoprotective effects [9][10], while the latter exhibit auspicious nephroprotective properties [11][12]. Hence, therapies endowed with pleiotropic and organoprotective attributes hold promise. Calpain is a stress-responsive intracellular protease, and its involvement in a variety of pathological conditions has been pointed out mainly through basic research since the early 2000s. This family of enzymes has been demonstrated to impart to atherosclerosis, as well as diabetes and hepatic affliction, thereby rendering it an exemplary molecular objective. A lot of chemical inhibitors targeting conventional calpain have been developed, and pharmaceutical companies have undertaken clinical investigations for diseases including Alzheimer’s disease and multiple sclerosis [13]. Given this context, calpain inhibitors have seemed clinically advantageous, and drug repositioning for cardiometabolic diseases may be considered promising. The therapeutic application of calpain inhibitors in cardiometabolic diseases is relatively behind, while recent basic research has elucidated the multifaceted actions of calpain in those metabolic disorders, indicating considerable anticipation for clinical applications.

2. Conventional Calpains and Calpastatin

Calpain designates a superfamily of cysteine proteases characterized by the presence of a CysPC motif within the gene (Figure 1). In mammals, there exist fifteen homologs of the catalytic subunits of calpains, each demonstrating diverse distributions and physiological functions in vivo [14][15][16][17][18]. Among these calpain isozymes, calpain-1 and calpain-2, categorized as conventional isozymes, have garnered global recognition since the 1970s [19][20]. Calpain-1 and calpain-2 form heterodimers by each binding with the common catalytic subunit, calpain-s1. They have undergone comprehensive examination using various methodologies across the fields of enzymology, physiology, and molecular pathology. Conventional calpains exhibit a notable sensitivity to calcium, a characteristic from which their nomenclature, “calpain”, is derived from “calcium” and “papain”. Their activity, however, is subject to regulation by extracellular stresses, including hypoxia, mechanical stresses, inflammatory cytokines, and growth factors, all mediated through the modulation of intracellular calcium levels [21]. In contrast, conventional calpains do not exhibit a strict recognition of a single amino acid sequence, and their substrate specificity remains relatively modest [22]. Conversely, the cleavage sites within each substrate exhibit precise delineation, frequently resulting in discrete fragments discernible as singular bands when subjected to Western blot analysis [23][24]. These substrate fragments may demonstrate divergent biological activities and stabilities compared to their full-length counterparts, thereby potentially influencing cellular functions. The distinct attributes of conventional calpains render them candidates for investigation in numerous disease etiologies. Nevertheless, unequivocally understanding their physiological functions is challenging due to the wide array of drivers and substrates.
Figure 1. Cardiometabolic calpains. Calpains are a superfamily of cysteine proteases with a CysPc domain. Two types of conventional isozymes and two types of unconventional calpains are thought to be involved in cardiometabolic diseases. Conventional calpains are formed when the subtype-specific catalytic subunits calpain-1 and calpain-2 form heterodimers with the common regulatory subunit, calpain-s1, respectively. On the other hand, no quaternary structure has been identified for calpain-6 and calpain-10, which are unconventional isoforms. Calpastatin is known as a specific endogenous inhibitor, and its increased expression in cells may downregulate the activity of conventional isozyme and calpain-10. Notably, a cysteine residue in the active core of calpain-6 is substituted with lysine and is thought to lack protease activity. CBSW, calpain-type β-sandwich domain; CysPc, cysteine protease domain, calpain-type; GR, glycine-rich domain; PC, protease core; PEF, penta-EF-hand domain.
Calpastatin is a specific endogenous calpain inhibitor and is frequently colocalized with calpain proteases [23][25][26]. Structurally, calpastatin comprises a tandem repeat of inhibitory domains that directly interact with and inhibit calpain activity. It is established that the individual inhibitory domains exhibit significant inhibitory efficacy, adequate for the suppression of calpain activity [25]. Dysregulation of calpain-calpastatin balance has been associated with several pathological conditions, such as neurodegenerative diseases (e.g., Alzheimer’s disease and Huntington’s disease) [27][28][29][30], tumor angiogenesis [23], and retinopathy [23]. In these contexts, aberrant calpastatin expression or function can lead to increased calpain activity, contributing to disease progression.

3. Calpain-10

Several unconventional calpains are also known to contribute to cardiometabolic diseases. Calpain-10 is expressed in various tissues, including pancreatic β-cells [31]. It is comprised of a calpain-type β-sandwich domain and microtubule-interacting and transport domain, as well as CysPC protease domains, while a penta-EF-hand domain is lacking [20]. It was documented that calpain-10 is associated with several diseases, most notably type 2 diabetes mellitus. Its involvement in type 2 diabetes mellitus is linked to genetic variations in the CAPN10 gene, which encodes calpain-10 [32][33][34].

4. Calpain-6

Calpain-6 is categorized as an unconventional calpain devoid of proteolytic activity due to the substitution of the cysteine residue within the active site of the CysPC domain with lysine. Its molecular configuration also diverges from conventional calpains, lacking the penta-EF-hand domain responsible for binding to the regulatory subunit [19][20]. Moreover, it has not been documented in a heterodimeric form. The expression profile of calpain-6 exhibits extraordinary distinctiveness. Experimental investigations in murine models have revealed embryonic expression within skeletal and cardiac muscle tissue [35]. Nonetheless, postnatally, this expression is anticipated to wane, with persistent expression primarily confined to the placenta [36]. Notably, Capn6 (calpain-6 gene)-deficient mice exhibit heightened skeletal muscle capacity and expedited repair of damaged skeletal muscle [36], suggesting potential functional implications of calpain-6 in skeletal muscle development.

References

  1. Sattar, N.; Gill, J.M.R.; Alazawi, W. Improving prevention strategies for cardiometabolic disease. Nat. Med. 2020, 26, 320–325.
  2. O’sullivan, J.W.; A Ashley, E.; Elliott, P.M. Polygenic risk scores for the prediction of cardiometabolic disease. Eur. Hear J. 2022, 44, 89–99.
  3. Ogata, H.; Takeshima, A.; Ito, H. An update on phosphate binders for the treatment of hyperphosphatemia in chronic kidney disease patients on dialysis: A review of safety profiles. Expert Opin. Drug Saf. 2022, 21, 947–955.
  4. Sarnak, M.J.; Amann, K.; Bangalore, S.; Cavalcante, J.L.; Charytan, D.M.; Craig, J.C.; Gill, J.S.; Hlatky, M.A.; Jardine, A.G.; Landmesser, U.; et al. Chronic kidney disease and coronary artery disease: JACC state-of-the-art review. J. Am. Coll. Cardiol. 2019, 74, 1823–1838.
  5. Muzurović, E.; Peng, C.C.-H.; Belanger, M.J.; Sanoudou, D.; Mikhailidis, D.P.; Mantzoros, C.S. Nonalcoholic Fatty Liver Disease and Cardiovascular Disease: A Review of Shared Cardiometabolic Risk Factors. Hypertension 2022, 79, 1319–1326.
  6. Bedogni, G.; Gastaldelli, A.; Foschi, F.G. Fatty liver, cardiometabolic disease, and mortality. Curr. Opin. Lipidol. 2021, 31, 27–31.
  7. Almeida, S.O.; Budoff, M. Effect of statins on atherosclerotic plaque. Trends Cardiovasc. Med. 2019, 29, 451–455.
  8. Oesterle, A.; Laufs, U.; Liao, J.K. Pleiotropic Effects of Statins on the Cardiovascular System. Circ. Res. 2017, 120, 229–243.
  9. Targher, G.; Mantovani, A.; Byrne, C.D. Mechanisms and possible hepatoprotective effects of glucagon-like peptide-1 receptor agonists and other incretin receptor agonists in non-alcoholic fatty liver disease. Lancet Gastroenterol. Hepatol. 2023, 8, 179–191.
  10. Brunner, K.T.; Henneberg, C.J.; Wilechansky, R.M.; Long, M.T. Nonalcoholic Fatty Liver Disease and Obesity Treatment. Curr. Obes. Rep. 2019, 8, 220–228.
  11. Marton, A.; Kaneko, T.; Kovalik, J.-P.; Yasui, A.; Nishiyama, A.; Kitada, K.; Titze, J. Organ protection by SGLT2 inhibitors: Role of metabolic energy and water conservation. Nat. Rev. Nephrol. 2020, 17, 65–77.
  12. Brown, E.; Heerspink, H.J.L.; Cuthbertson, D.J.; Wilding, J.P.H. SGLT2 inhibitors and GLP-1 receptor agonists: Established and emerging indications. Lancet 2021, 398, 262–276.
  13. Ono, Y.; Saido, T.C.; Sorimachi, H. Calpain research for drug discovery: Challenges and potential. Nat. Rev. Drug Discov. 2016, 15, 854–876.
  14. Miyazaki, T.; Miyazaki, A. Emerging roles of calpain proteolytic systems in macrophage cholesterol handling. Cell. Mol. Life Sci. 2017, 74, 3011–3021.
  15. Miyazaki, T.; Akasu, R.; Miyazaki, A. Calpain-Associated Proteolytic Regulation of the Stromal Microenvironment in Cancer. Curr. Pharm. Des. 2021, 27, 3128–3138.
  16. Miyazaki, T.; Miyazaki, A. Impact of Dysfunctional Protein Catabolism on Macrophage Cholesterol Handling. Curr. Med. Chem. 2019, 26, 1631–1643.
  17. Miyazaki, T.; Miyazaki, A. Defective Protein Catabolism in Atherosclerotic Vascular Inflammation. Front. Cardiovasc. Med. 2017, 4, 79.
  18. Miyazaki, T.; Akasu, R.; Miyazaki, A. Calpain proteolytic systems counteract endothelial cell adaptation to inflammatory en-vironments. Inflamm. Regen. 2020, 40, 5.
  19. Goll, D.E.; Thompson, V.F.; Li, H.; Wei, W.; Cong, J. The calpain system. Physiol. Rev. 2003, 83, 731–801.
  20. Ono, Y.; Sorimachi, H. Calpains: An elaborate proteolytic system. Biochim. Biophys. Acta 2012, 1824, 224−236.
  21. Miyazaki, T.; Miyazaki, A. Dysregulation of Calpain Proteolytic Systems Underlies Degenerative Vascular Disorders. J. Atheroscler. Thromb. 2018, 25, 1–15.
  22. Shinkai-Ouchi, F.; Koyama, S.; Ono, Y.; Hata, S.; Ojima, K.; Shindo, M.; Duverle, D.; Ueno, M.; Kitamura, F.; Doi, N.; et al. Predictions of Cleavability of Calpain Proteolysis by Quantitative Structure-Activity Relationship Analysis Using Newly Determined Cleavage Sites and Catalytic Efficiencies of an Oligopeptide Array. Mol. Cell. Proteom. 2016, 15, 1262–1280.
  23. Miyazaki, T.; Taketomi, Y.; Saito, Y.; Hosono, T.; Lei, X.-F.; Kim-Kaneyama, J.-R.; Arata, S.; Takahashi, H.; Murakami, M.; Miyazaki, A. Calpastatin Counteracts Pathological Angiogenesis by Inhibiting Suppressor of Cytokine Signaling 3 Degradation in Vascular Endothelial Cells. Circ. Res. 2015, 116, 1170–1181.
  24. Barefield, D.Y.; McNamara, J.W.; Lynch, T.L.; Kuster, D.W.; Govindan, S.; Haar, L.; Wang, Y.; Taylor, E.N.; Lorenz, J.N.; Nieman, M.L.; et al. Ablation of the calpain-targeted site in cardiac myosin binding protein-C is cardioprotective during ischemia-reperfusion injury. J. Mol. Cell. Cardiol. 2019, 129, 236–246.
  25. Maki, M.; Takano, E.; Mori, H.; Sato, A.; Murachi, T.; Hatanaka, M. All four internally repetitive domains of pig calpastatin possess inhibitory activities against calpains I and II. FEBS Lett. 1987, 223, 174–180.
  26. Takano, E.; Murachi, T. Purification and Some Properties of Human Erythrocyte Calpastatin1. J. Biochem. 1982, 92, 2021–2028.
  27. Morales-Corraliza, J.; Berger, J.D.; Mazzella, M.J.; Veeranna; Neubert, T.A.; Ghiso, J.; Rao, M.V.; Staufenbiel, M.; Nixon, R.A.; Mathews, P.M. Calpastatin modulates APP processing in the brains of β-amyloid depositing but not wild-type mice. Neurobiol. Aging 2012, 33, 1125.e9–1125.e18.
  28. Sato, K.; Minegishi, S.; Takano, J.; Plattner, F.; Saito, T.; Asada, A.; Kawahara, H.; Iwata, N.; Saido, T.C.; Hisanaga, S.-I. Calpastatin, an endogenous calpain-inhibitor protein, regulates the cleavage of the Cdk5 activator p35 to p25. J. Neurochem. 2011, 117, 504–515.
  29. Higuchi, M.; Iwata, N.; Matsuba, Y.; Takano, J.; Suemoto, T.; Maeda, J.; Ji, B.; Ono, M.; Staufenbiel, M.; Suhara, T.; et al. Mechanistic involvement of the calpain-calpastatin system in Alzheimer neuropathology. FASEB J. 2011, 26, 1204–1217.
  30. Weber, J.J.; Kloock, S.J.; Nagel, M.; Ortiz-Rios, M.M.; Hofmann, J.; Riess, O.; Nguyen, H.P. Calpastatin ablation aggravates the molecular phenotype in cell and animal models of Huntington disease. Neuropharmacology 2018, 133, 94–106.
  31. Ling, C.; Groop, L.; Guerra, S.D.; Lupi, R. Calpain-10 expression is elevated in pancreatic islets from patients with type 2 diabetes. PLoS ONE 2009, 4, e6558.
  32. Horikawa, Y.; Oda, N.; Cox, N.J.; Li, X.; Orho-Melander, M.; Hara, M.; Hinokio, Y.; Lindner, T.H.; Mashima, H.; Schwarz, P.E.; et al. Genetic variation in the gene encoding calpain-10 is associated with type 2 diabetes mellitus. Nat. Genet. 2000, 26, 163–175.
  33. Bayramci, N.S.; Açik, L.; Kalkan, C.; Yetkin, I. Investigation of glucocorticoid receptor and calpain-10 gene polymorphisms in Turkish patients with type 2 diabetes mellitus. Turk. J. Med Sci. 2017, 47, 1568–1575.
  34. Karambataki, M.; Malousi, A.; Tzimagiorgis, G.; Haitoglou, C.; Fragou, A.; Georgiou, E.; Papadopoulou, F.; Krassas, G.E.; Kouidou, S. Association of two synonymous splicing-associated CpG single nucleotide polymorphisms in calpain 10 and solute carrier family 2 member 2 with type 2 diabetes. Biomed. Rep. 2016, 6, 146–158.
  35. Tonami, K.; Hata, S.; Ojima, K.; Ono, Y.; Kurihara, Y.; Amano, T.; Sato, T.; Kawamura, Y.; Kurihara, H.; Sorimachi, H. Cal-pain-6 deficiency promotes skeletal muscle development and regeneration. PLoS Genet. 2013, 9, e1003668.
  36. Dear, N.; Matena, K.; Vingron, M.; Boehm, T. A new subfamily of vertebrate calpains lacking a calmodulin-like domain: Implications for calpain regulation and evolution. Genomics 1997, 45, 175–184.
More
Information
Subjects: Pathology
Contributor MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register :
View Times: 146
Revisions: 2 times (View History)
Update Date: 07 Dec 2023
1000/1000
Video Production Service