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Martins, M.S.; Gonçalves, A.C.; Alves, G.; Silva, L.R. Blackberries and Mulberries. Encyclopedia. Available online: https://encyclopedia.pub/entry/47599 (accessed on 08 August 2024).
Martins MS, Gonçalves AC, Alves G, Silva LR. Blackberries and Mulberries. Encyclopedia. Available at: https://encyclopedia.pub/entry/47599. Accessed August 08, 2024.
Martins, Mariana S., Ana C. Gonçalves, Gilberto Alves, Luís R. Silva. "Blackberries and Mulberries" Encyclopedia, https://encyclopedia.pub/entry/47599 (accessed August 08, 2024).
Martins, M.S., Gonçalves, A.C., Alves, G., & Silva, L.R. (2023, August 03). Blackberries and Mulberries. In Encyclopedia. https://encyclopedia.pub/entry/47599
Martins, Mariana S., et al. "Blackberries and Mulberries." Encyclopedia. Web. 03 August, 2023.
Blackberries and Mulberries
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This entry is adapted from the peer-reviewed paper 10.3390/ijms241512024

Blackberries and mulberries are small and perishable fruits that provide significant health benefits when consumed. In reality, both are rich in phytochemicals, such as phenolics and volatile compounds, and micronutrients, such as vitamins. All the compounds are well-known thanks to their medicinal and pharmacological properties, namely antioxidant, anti-inflammatory, anti-cancer, antiviral, and cardiovascular properties. Nevertheless, variables such as genotype, production conditions, fruit ripening stage, harvesting time, post-harvest storage, and climate conditions influence their nutritional composition and economic value. Given these facts, researchers focus on the nutritional and chemical composition, as well as the health benefits, of two blackberry species (Rubus fruticosus L., and Rubus ulmifolius Schott) and one mulberry species (Morus nigra L.).

blackberry mulberry phytochemicals phenolic compounds health-promoting properties

1. Antidiabetic Properties

Diabetes mellitus is a chronic endocrine condition in which the pancreas either stops producing insulin or produces inadequate insulin. Diabetes affects about 425 million people globally and is defined by a rise in blood glucose concentration (>7 mmol/L) [1]. It has been associated with the development of various significant problems at cardiovascular, neurological, and renal levels, leading to increased morbidity and mortality [2]. The International Diabetes Federation anticipated that, by 2030, there will be 552 million diabetics globally [3].
To establish glycemic control, diabetics use insulin and other therapy drugs, such as metformin, sodium-glucose cotransporter-2 inhibitors, and glucagon-like peptide 1 [4]. Before the development of insulin, medicinal plants were used to treat this condition. Because of their low cost, availability, and lack of negative effects, the use of natural plants was and still is an alternative for many people. Various plant genera and phytochemical constituent types with anti-diabetic properties have been used in this context [4][5][6][7]. Therefore, it is not surprising that formulations using anti-diabetic plant extracts or phytocompounds have been derived. Additionally, nowadays, systems such as “Herbal-based anti-diabetic drug delivery systems” are largely used to provide herbal medicines to treat diabetes [7].
Certain regions of the world employ black mulberry leaves, fruits, and barks as anti-diabetic medications, believing in their efficacy in lowering blood glucose levels [5][8][9][10][11]. In accordance with this, Morus nigra has been shown to have a wide range of biological and pharmacological therapeutic benefits, including antidiabetic, anti-obesity, and anti-hyperlipidemic effects [12]. Hydroethanolic freeze-dried extracts of this fruit revealed potential for inhibiting pancreatic lipase, displaying a half maximal inhibitory concentration of 6.32 mg/mL [13].
Among both berries’ constituents, quercetin has been demonstrated to have considerable antioxidant and anti-inflammatory characteristics and the ability to interfere with a variety of antidiabetic activities, including insulin secretion and sensitization, glucose level improvement, and inhibition of intestinal glucose absorption. By activating adenosine monophosphate and preventing lipid peroxidation, this phenolic molecule promotes glucose transporter 4, the principal facilitative mediator of glucose uptake in skeletal muscles, adipose tissues, and other peripheral tissues. Given that, it is not surprising that quercetin can be used to stabilize blood glucose and body weight [14][15]. Furthermore, a single oral dosage of quercetin (400 mg) decreased α-glucosidase activity and reduced postprandial hyperglycemia in rats with type 2 diabetes [16].
Ferulic acid, another berry phenolic component, at 1000 mg per day for six weeks, showed the capacity to decrease total cholesterol, malonylaldehyde, TNF-α, and triglycerides by 8.1, 24.5, 13.1, and 12.1%, respectively, and increase HDL cholesterol by 4.3% [17]. These findings suggested that ferulic acid can also help diabetic patients with hyperlipidemia. Ferulic acid was found to be generally safe, with LD50 values of 2445 mg /kg in male rats and 2113 mg /kg in female rats [18].
Additionally, diabetic male Wistar rats received injections of black mulberry fruit extracts at 150 and 300 mg/kg body weight for 4 weeks. After this time, microalbuminuria, albumin, glucose, insulin, creatinine, and creatine levels in the serum were measured. The study discovered that diabetic animals considerably improved in all of the measures tested. The activity of catalase activity was also improved. Furthermore, the histological examination of their kidney tissues revealed a significant reduction in degenerative anomalies and glomerular sclerosis. TNF-α, vascular cell adhesion molecule-1, and fibronectin mRNA expression were all downregulated in treated rats [10]. Therefore, the downregulation of TNF-α, VCAM-1, and fibronectin levels in diabetic rats avoids, or retards, the development of diabetic nephropathy. Altogether, these data support the evidence that mulberry fruit extract may be a potential agent in the treatment of diabetic nephropathy [12].

2. Antimicrobial Properties

Plant-derived antimicrobial chemicals may limit the development of bacteria, fungi, viruses, and protozoa by different processes from those utilized by synthetic antimicrobials, and thus exhibit substantial therapeutic benefit in the treatment of resistant microbial strains. The antimicrobial activity of an agent is generally due its potential to chemically interfere with the manufacture or function of key components of bacteria and/or evade established antibacterial resistance mechanisms [6][19][20].
The majority of phytochemicals with therapeutic value found in fruits are secondary metabolites. Their antimicrobial activity varies depending on the structure, number, and position of substituent groups, the presence of glycosidic linkages, and the alkylation of hydroxyl groups [20][21]. As expected, blackberries’ antimicrobial properties differ among cultivars and ambient and soil factors. Furthermore, it is important to note that it is not possible to associate the antimicrobial activity with a specific compound due to the capacity of phenolic compounds to act synergistically [5][22][23][24][25][26][27][28][29].
Recent research has revealed that blackberries and mulberries have notable antimicrobial properties. The antimicrobial activity of different R. fruticosus extracts was investigated against Escherichia coli, Staphylococcus aureus, Bacillus cereus, B. subtilis, B. mojavensis, Salmonella Hartford, Proteus vulgaris, Pseudomonas baetica, Micrococcus luteus, and Saccharomyces cerevisiae. The inhibition zone diameter (mm) was measured, revealing that the ethanolic extracts are more competitive than the crude extracts, and show a notable antimicrobial potential against Proteus vulgari (20.53 mm). The lowest activity was observed against S. Hartford bacteria (9.54 mm). Minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were not calculated [24].
Additionally, hydroethanolic extracts of R. ulmifolius proved to have bacteriostatic effects against three Gram-negative bacteria (E. coli, Morganella morganii, and P. mirabilis), four Gram-positive bacteria (MRSA-methicillin-resistant S. aureus, MSSA-methicillin susceptible S. aureus, Listeria monocytogenes, and Enterococcus faecalis), and one fungus (Candida albicans). The results revealed activity in some tested strains, with MIC values ranging between 5 and >20 mg/mL. To inhibit the growth of Klebsiella pneumoniae and Pseudomonas aeruginosa, a concentration above 20 mg/mL was necessary. For the remaining Gram-negative strains, the most effective results were shown against M. morganii (MIC = 5 mg/mL) and E. coli (MIC = 5 mg/mL), followed by P. mirabilis (10 mg/mL) [30]. In another study, methanolic extracts of R. ulmifolius showed antimicrobial potential against two Gram-negative bacteria (E. coli and Salmonella typhimurium), three Gram-positive bacteria (S. aureus, Enterococcus feacium, Streptococcus agalactiae) and one fungus (Candida albicans). The most notable values were observed against S. agalactiae and E. coli bacteria [25].
The antimicrobial effects of M. nigra were also evaluated, especially in S. aureus, P. aeruginosa, and E. coli, where the ability of its extracts to inhibit the production of proinflammatory cytokines and interfere with iNOS and NF-κB pathways was observed [25]. Considering the higher content of anthocyanins in this species, these effects could be attributed to these compounds. In fact, anthocyanins have potent antiviral and antibacterial properties, being already known for their antimicrobial potential against K. pneumonia, P. aeruginosa, S. aureus, E. coli, H1N1, SARS-CoV-2, and rabies and herpes simplex virus [6][21][25].
Additionally, the antibacterial efficacy of mulberry total flavonols was assessed against three bacteria (E. coli, P. aeruginosa, and S. aureus), revealing interesting MBC results against S. aureus and E. coli [26]. Another investigation demonstrated the potential of M. nigra ethanolic extracts to be used in acne-treatment beauty care products given their capacity to inhibit S. epidermis and P. acnes growth, revealing MIC values of 2.5% for both bacteria, and MBC scores of 2.5% and 5% against S. epidermidis and P. acnes, respectively [27].
Black mulberry juice also has antibacterial properties, with its ability against three Gram-negative strains (E. coli, P. aeruginosa, and S. typhimurium) and five Gram-positive strains (Bacillus spizizenii, B. subtilis, Corynebacterium diphtheriae, Enterococcus. faecalis, and S. aureus) being previously reported. The maximum zone of inhibition was against P. aeruginosa (19.87 mm), followed by Bacillus spizizenii (19.68 mm) and B. subtilis (18.46 mm). The minimum zone of inhibition was obtained against E. coli (9.98 mm). Among the Gram-positive species, Bacillus species exhibited the highest zones of inhibition while, regarding Gram-negative bacteria, P. aeruginosa had higher inhibition than S. typhimurium and E. coli [28].

3. Antioxidant Activity

Reactive species are products of normal cellular metabolism and play key roles in signal transduction pathways, growth regulation, gene expression, and immune responses. In the human body, various mechanisms are necessary to maintain redox homeostasis [6][31]. These mechanisms include non-enzymatic and enzymatic antioxidant defenses created in the body (endogenous), as well as those given by the food (exogenous). However, the overproduction and accumulation of free radicals can lead to oxidative damage [32]. This biological condition may be caused by a lack of antioxidant defense mechanisms, excessive reactive species production, and excessive activation of their systems, increasing aging and the pathology of many chronic diseases, such as cancer, cardiovascular disease, inflammation, diabetes, and Parkinson’s and Alzheimer’s disease [6][33]. Therefore, it is essential to reduce their levels. Flavonoids, stilbenes, and tannins are examples of exogenous antioxidants. For example, scavenging and detoxifying radical oxygen species and preventing their production, influencing the cell cycle, avoiding tumor suppression, and modulating signal transduction, apoptosis events, and metabolism, are all biologically relevant mechanisms attributed to phenolic compounds [6][34][35][36][37][38][39][40]. Their antioxidant diversity and concentration are greatly dependent on the species and cultivars. Pre-harvest practices, environmental conditions, harvest ripeness, postharvest storage, and processing operations are also key drivers of phytochemical profiles [34][41][42].
Blackberries are considered one of the richest sources of natural antioxidants due to their high content of phenolic compounds, such as anthocyanins, ellagitannins, flavonols, and flavanols [30][43][44][45][46][47]. In fact, they present an extraordinary capacity to scavenge chemically generated radicals, thus preventing a wide range of human disorders and maintaining a healthy balance between free radicals and antioxidant systems. In particular, blackberries have notable antioxidant abilities against superoxide radicals (O2●−), hydrogen peroxide (H2O2), hydroxyl radicals (●OH), and singlet oxygen (1O2) [39].
The antioxidant capacity of blackberries was previously determined by in vitro assays, by the lipid peroxidation inhibition assays (TBARS), oxidative reactive oxygen and nitrogen species (ROS/RNS), hemolysis inhibition assay, the ORAC method, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS•+), the ferric-reducing/antioxidant power (FRAP) method, 2,2-diphenylpicrylhydrazyl (DPPH•), and Trolox equivalent antioxidant capacity (TEAC) assay [22][22][45][48][49][50][51][52][53][54][55][56][57][58][59][60][61].
In the TBARS experiment, R. fruticosus extract revealed a high antioxidant activity, displaying an IC50 value of 100 ug/mL, which is substantially lower than that obtained with the positive control, Trolox (139 ug/mL) [45]. Additionally, using FRAP assay, ABTS•+, and DPPH•, the obtained results were between 4.45–14.16 for FRAP, 2.28–8.89 for ABTS•+, and 2.63–9.35 mmol Trolox equivalents per⋅100 g fw for DPPH• [53].
Furthermore, methanolic extracts of M. nigra at 76 µg showed the capacity to inhibit lipid oxidation by 28.7%, while ethanolic extracts exhibited lower inhibitory capacity (23.7–47.6%) [54]. The antioxidant abilities of its aqueous extracts were also evaluated, revealing lower abilities than the methanolic ones; at 100 µg/mL, the values obtained were 1.1% and 7.1% for aqueous and methanolic extracts, respectively, whereas at 300 µg/mL, the corresponding values were 7.1% and 21.6%, respectively [54].
However, when comparing wild blackberries (R. ulmifolius) with the cultivated ones (R. fruticosus), substantial differences were found, with the latter having higher antioxidant content [55].
To summarize, mulberries have lately gained a large amount of interest as prospective sources of functional foods due to a variety of biological benefits [12][62]. The obtained findings on the antioxidant activity of mulberry fruits support their incorporation in biological applications [9][12][54][59].

4. Anti-Inflammatory Properties

Inflammation is the immune system’s reaction to potentially damaging stimuli such as infection or injury. In the presence of stressors, immune cells release inflammatory substances, such as inflammatory cytokines, including TNF-α and interleukins (IL)-6 and 10, leading to increased nitric oxide (NO) levels and prostaglandins via the catalysis of cyclooxygenase-2 (Cox2) and NF-κB pathways [6][63][64]. Blackberry freeze-dried powders are capable of reducing mRNA expression of NF-kB and COX-2 in the liver [65].
A healthy lifestyle that includes physical activity, stopping smoking, and moderate alcohol intake, associated with a diet rich in fruits, vegetables, and whole grains, decreases the risk of developing chronic diseases. As expected, phenolic compounds, carotenoids, vitamins, and dietary fiber contribute to the anti-inflammatory and antioxidant effects of fruits and vegetables [6][66][67][68][69]. In particular, high quantities of dietary anthocyanins may be viewed as a feasible nutraceutical in the context of inflammatory disease. Among these, cyanidin 3-O-glucoside can reduce cytokine-induced inflammation in intestinal cells by inhibiting the production of NO, PGE2, and IL-8, and the expression of iNOS and COX-2 [21][68][69][70].
Focusing on blackberries and mulberries, anthocyanin-enriched fractions from fermented blueberry and blackberry beverages inhibited dipeptidyl peptidase-IV activity in LPS-stimulated murine macrophages. Computational docking demonstrated that this effect could be mainly attributed to delphinidin 3-O-arabinoside, which effectively inactivates dipeptidyl peptidase-IV by binding with a low interaction energy (−3228 kcal/mol). Additionally, anthocyanins and proanthocyanidins (100 µM cyanidin 3-O-glucoside and epicatechin equivalents, respectively) extracted from them reduced LPS-induced inflammatory response in mouse macrophages by stopping the NF-κB pathway [70]. Another study that used RAW 264.7 macrophages stimulated with LPS demonstrated that blackberry anthocyanin extract (0–20 µg/mL)-treated macrophages presented lower levels of IL-1 and TNF-α [71]. Once again, this reduction is mainly associated with the ability of anthocyanins to interfere with NF-κB signaling [70], particularly of cyanidin 3-O-glucoside, which previously showed potential to decrease pro-inflammatory mediators NO, PGE2, COX-2, and IL-8 generated by cytokine-stimulated HT-29 cells [69]. In accordance with this, R. fruticosus also showed capacity to inhibit the secretion of pro-inflammatory IL-8 cytokines in two cellular models (HT-29 and T-84 cells) in a dose dependent-manner in both cell lines [23].
Ellagitannins are another significant polyphenol that has displayed anti-inflammatory properties. Previous research [72] examined their anti-inflammatory efficacy of TNF-α, IL-1B, IL-8, and NF-κB on the AGS gastric cell line. Ellagitannins extracted from R. fruticosus suppressed TNF-α, showing an IC50 value of 0.67–1.73 mg/mL. At 2 mg/mL, ellagitannins inhibited TNF-α and NF-κB nuclear translocation by 57% and 67%, respectively. At lower doses, ellagitannins reduced IL-8 secretion, revealing an IC50 ranging between 0.7 and 4 mg/mL. Moreover, in a rat model of ethanol-induced stomach lesions, the protective effect of ellagitannins was also tested. Ellagitannins (20 mg/kg/day) were administered orally to rats for ten days, and ethanol was administered one hour before sacrifice. The mucosa of the stomach was separated and utilized to measure IL-8 release, NF-κB nuclear translocation, TEAC, and superoxide dismutase and catalase activities. This investigation demonstrates that the treatment with these compounds can decrease NF-κB nuclear translocation and suppress IL-8 production. The present work demonstrated that ellagitannins derived from Rubus berries definitively protect against the formation of gastric ulcers in rat animal models. In particular, ellagitannins can block the NF-κB cascade either directly on the cell response to pro-inflammatory cytokines or act as antioxidant agents by inhibiting reactive species generated in several inflammatory conditions [73].

5. Neuroprotection

The human brain is responsible for a wide range of cognitive, motor and behavioral functions that require significant amounts of energy. Neurons are responsible for transmitting information to and from the brain. Neurodegenerative illnesses are distinguished by progressive brain cell death and neuronal loss, which impair motor or cognitive function. Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, amyotrophic lateral sclerosis, and spinocerebellar ataxia are examples of common neurodegenerative disorders. These disorders are a major public health concern, particularly among the elderly [33]. These disorders develop because the brain is more sensitive to oxidative stress than other organs due to the poor activity of antioxidant defense mechanisms [74].
Many epidemiological studies are being conducted to study the potential of phenolics to be used to promote neuronal health and prevent neural cells from being damaged through their antioxidant and anti-inflammatory properties, and thus delay Parkinson’s and Alzheimer’s diseases, ischemic diseases, and aging effects [67][75].
The inclusion of blackberries in the diet has been shown to reduce brain degeneration [2][76][77][78][79]. The neuroprotective capacity of this fruit mainly comes from its antioxidant capacity, promoted by the presence of phenolic compounds, such as anthocyanins, caffeic acid, and quercetin [6][67][79][80][81][82]. Indeed, these compounds can penetrate the hematoencephalic membrane and have neuroprotective effects on various cerebral structures in the brain, including the hippocampus and cortex [38]. Flavonoids may also have important impacts on mammalian cognitive function, perhaps halting the aging-related declines in memory and learning. These benefits are mostly sought for preventing brain damage, such as neurodegenerative diseases, and improving memory, learning, and cognitive functions [79]. Blackberries from the north of Portugal can lower intracellular reactive species levels, alter glutathione levels, and inhibit the emergence of caspases during treatment, hence reducing oxidative stress and preventing neurodegeneration [78]. Mulberry fruit extracts and cyanidin 3-O-glucoside have shown the ability to inhibit reactive species production and, consequently, neuronal injury [83][84].
Neuroblastoma cells exposed to H2O2 and treated with raw, digested, and dialyzed blackberry extracts at physiological concentrations revealed lower age-related neurodegeneration [76]. In addition, animal research found that an intermediate dosage of blackberry juice (5.83 mg/kg anthocyanins, 27.10 mg/kg polyphenols) enhanced mechanisms of behavioral coping with diazepam l. The forced swim test supported these findings by demonstrating that blackberry juice, at moderate and high doses, improves the acute stress response [85]. These findings suggest that blackberry juice may have a therapeutic value in alleviating anxiety caused by stressful experiences.
M. nigra revealed a notable protective effect against Alzheimer’s disease, specifically by inhibiting amyloid-β-induced paralysis symptoms and suppressing over-sensitivity to exogenous serotonin by about 55.65% in transgenic Alzheimer’s disease Caenorhabditis elegans models, which were treated with up to 1.00 mg/mL. These effects are due to the capacity of this fruit to activate the DAF-16/SOD-3/GST-4 pathway, improve antioxidant capacity, delay aging, and alleviate the symptoms caused by the amyloid-β protein [86]. These findings suggest that functional foods, such as mulberry, can be used to lower the risk of Alzheimer’s disease.

6. Anticancer Activity

Cancers are characterized by abnormal cell growth capable of invading other regions of the body, resulting in metastasis. A tumor is a complex multistage process that begins with the genesis of a cancer cell caused by DNA damage, followed by accumulation of mutations, progression to cell proliferation and tumor expansion, and, finally, progression to malignancy and metastasis. While new cancer incidence is expected to rise by 70% by 2034, approximately 35% of cancer deaths are attributed to behavioral and dietary risks, such as high body mass index, low fruit and vegetable intake, and lack of physical activity [87].
According to epidemiological and clinical research, a diet consisting of 400–800 g of various vegetables and fruits per day can prevent 20% or more of all cancer cases [66][67][88].
Phenolic berry content has shown the capacity to reduce inflammation, inhibit angiogenesis, protect against DNA damage, and influence apoptosis or proliferation rates in malignant cells. Indeed, they demonstrate the ability to interfere in all phases of cancer development, including initiation, promotion, progression, invasion, and metastasis [6][63][82][89]. Berry extracts also inhibited cancer-induced AP-1 and NF-κB, as well as decreasing the expression of the two proteins involved in tumor promotion and progression, i.e., vascular endothelial growth factor and COX2 [65][90]. These effects are intimately linked to the capacity of phenolics to alter the genomic stability at many phases in the cancer genesis process [67]. For example, anthocyanins have been shown to activate phase II enzymes, which may inactivate carcinogens triggered by phase I enzymes, and hence prevent DNA damage caused by the carcinogens [21][40][91][92].
Dietary bioactive compounds can also decrease telomerase activity by modifying histones or by inhibiting DNA methyltransferases. Telomerase activity has been detected in more than 80% of human malignancies, making the enzyme a promising target for anticancer treatment. According to research, the antiproliferative impact of blackberry fruits is mediated by their anti-telomerase activity [93]. Additionally, there have been no negative effects associated with the administration of blackberries, indicating that this fruit has the potential to be effective for a dietary plan to reduce cancer risk and assist cancer patients with illness prognosis [90].
Blackberries previously demonstrated significant chemo-preventative and antioxidant activities by inhibiting the growth, proliferation, and migration of the human A549 lung carcinoma cell line, and strong inhibitory effects on the cell growth of highly metastatic breast cancer HS578T cells, by inducing significant alterations in cell cycle regulators, causing G2/M arrests [94]. Blackberries and mulberries contain anthocyanin cyanidin-3-O-glucoside, which has promising qualities for usage in nutraceuticals, and has shown potential to limit cell proliferation, arresting the cell cycle in the G2/M phase, and inducing apoptosis in vitro [21][68][95]. In fact, in a recent investigation, rats were administered orally with purified cyanidin 3-O-glucoside (800 µmol/kg of body weight). After 30 min–2.0 h of delivery, this was detected in plasma, with a Cmax value of 0.8 µM. This evidence represents added value regarding the incorporation of this anthocyanin in dietary supplements, aiding in the anticancer therapy of breast cancer [95].
Morus nigra extracts have also been the subject of much research. A three-month enriched diet applied in MUC2−/− mice, with a model of spontaneous chronic intestinal inflammation and induced-intestinal tumors at three months, at 5% or 10%, resulted in a reduction in tumorigenesis and intestinal inflammation. Basically, mice aged 6 to 8 weeks that were supplemented with 5% or 10% M. nigra extracts for 10 days and there were observed improvements in their signs and symptoms caused by dextran sulfate sodium-induced acute colitis, preventing weight loss and bloody stools, and promoting positive changes in the histology of the colorectal lining [96].

7. Cardiovascular Protection

Cardiovascular disorders affect the heart and blood vessels and are the major cause of death worldwide. People who have high blood pressure and cholesterol, as well as smokers, those who are sedentary or obese, and people who have a diet rich in salt, sugar, and fatty acids, are more susceptible to cardiovascular problems [97].
The current nutritional guidelines for the prevention of cardiovascular diseases include a Mediterranean-style diet rich in fruits, vegetables, and whole grains, as well as non-tropical vegetable oils, in order to reduce total cholesterol, oxidative stress, and inflammation [66][67][80][88][98][99].
Blackberry phenolic compounds have demonstrated the capacity to diminish LDL oxidation, quench free radicals by hydrogen molecule donation, and interfere with liposome oxidation systems [100][101]. In particular, anthocyanins from M. nigra showed the capacity to protect human primary endothelial cells by decreasing the production of the cytokine-induced chemokine monocyte chemotactic protein 1, a protein directly linked to atherogenesis, and which is mainly responsible for attracting macrophages to sites of infection or inflammation [102]. Moreover, although not directly shown in blackberry flavonoids, several flavonoids also revealed the capacity to protect platelet function, which is crucial in the pathogenesis of these diseases. In fact, flavonoids can minimize platelet aggregation, reduce platelet generation of superoxide anions, and increase platelet NO production [103].
In epidemiological studies, diets high in plant-derived phenolic compounds have been shown to reduce the incidence of coronary heart disease. The chronic antioxidant and hypolipidemic characteristics of these compounds play critical roles in the prevention of lipoprotein oxidation and the formation of atherosclerotic lesions [38][88][101][104].

References

  1. Salau, V.F.; Erukainure, O.L.; Islam, M.S. Phenolics: Therapeutic applications against oxidative injury in obesity and type 2 diabetes pathology. In Pathology-Oxidative Stress and Dietary Antioxidants; Victor, R.P., Ed.; Academic Press: Cambridge, MA, USA, 2020; pp. 297–307.
  2. Manzoor, M.F.; Hussain, A.; Tazeddinova, D.; Abylgazinova, A.; Xu, B. Assessing the nutritional-value-based therapeutic potentials and non-destructive approaches for mulberry fruit assessment: An overview. Comput. Intell. Neurosci. 2022.
  3. Saeedi, P.; Petersohn, I.; Salpea, P.; Malanda, B.; Karuranga, S.; Unwin, N.; Colagiuri, S.; Guariguata, L.; Motala, A.A.; Ogurtsova, K.; et al. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas, 9th edition. Diabetes Res Clin Pr. 2019, 157, 107843.
  4. Taylor, S.I.; Yazdi, Z.S.; Beitelshees, A.L. Pharmacological treatment of hyperglycemia in type 2 diabetes. J. Clin. Invest. 2021, 131, 1–14.
  5. Weli, A.M.; Al-Saadi, H.S.; Al-Fudhaili, R.S.; Hossain, A.; Putit, Z.B.; Jasim, M.K. Cytotoxic and antimicrobial potential of different leaves extracts of R. fruticosus used traditionally to treat diabetes. Toxicol. Rep. 2020, 7, 183–187.
  6. Gonçalves, A.C.; Bento, C.; Silva, B.; Simões, M.; Silva, L.R. Nutrients, bioactive compounds and bioactivity: The health benefits of sweet cherries (Prunus avium L.). Curr. Nutr. Food Sci. 2019, 15, 208–227.
  7. Kambale, E.K.; Quetin-Leclercq, J.; Memvanga, P.B.; Beloqui, A. An overview of herbal-based antidiabetic drug delivery systems: Focus on lipid- and inorganic-based nanoformulations. Pharmaceutics 2022, 14, 2135.
  8. Volpato, G.T.; Calderon, I.M.P.; Sinzato, S.; Campos, K.E.; Rudge, M.V.C.; Damasceno, D.C. Effect of Morus nigra aqueous extract treatment on the maternal-fetal outcome, oxidative stress status and lipid profile of streptozotocin-induced diabetic rats. J. Ethnopharmacol. 2011, 138, 691–696.
  9. Samappito, S.; Butkhup, L. Effect of skin contact treatments on the aroma profile and chemical components of mulberry (Morus alba Linn.) wines. Afr. J. Food Sci. 2010, 4, 52–61.
  10. Abouzed, T.K.; Sadek, K.M.; Ghazy, E.W.; Abdo, W.; Kassab, M.A.; Hago, S.; Abdel-Wahab, S.; Mahrous, E.A.; Abdel-Sattar, E.; Assar, D.H. Black mulberry fruit extract alleviates streptozotocin-induced diabetic nephropathy in rats: Targeting TNF-α inflammatory pathway. J. Pharm. Pharmacol. 2020, 72, 1615–1628.
  11. Ştefănuţ, M.N.; Căta, A.; Pop, R.; Tănasie, C.; Boc, D.; Ienaşcu, I.; Ordodi, V. Anti-hyperglycemic effect of bilberry, blackberry and mulberry ultrasonic extracts on diabetic rats. Plant Foods Hum. Nutr. 2013, 68, 378–384.
  12. Lim, S.H.; Choi, C.I. Pharmacological properties of Morus nigra L. (Black mulberry) as a promising nutraceutical resource. Nutrients 2019, 11, 1048.
  13. Fabroni, S.; Ballistreri, G.; Amenta, M.; Romeo, F.V.; Rapisarda, P. Screening of the anthocyanin profile and in vitro pancreatic lipase inhibition by anthocyanin-containing extracts of fruits, vegetables, legumes and cereals. J. Sci. Food Agric. 2016, 96, 4713–4723.
  14. Xu, M.; Hu, J.; Zhao, W.; Gao, X.; Jiang, C.; Liu, K.; Liu, B.; Huang, F. Quercetin differently regulates insulin-mediated glucose transporter 4 translocation under basal and inflammatory conditions in adipocytes. Mol. Nutr. Food Res. 2014, 58, 931–941.
  15. Yi, H.; Peng, H.; Wu, X.; Xu, X.; Kuang, T.; Zhang, J.; Du, L.; Fan, G. The therapeutic effects and mechanisms of quercetin on metabolic diseases: Pharmacological data and clinical evidence. Oxid. Med. Cell. Longev. 2021, 2021, 1–16.
  16. Kim, J.H.; Kang, M.J.; Choi, H.N.; Jeong, S.M.; Lee, Y.M.; Kim, J.I. Quercetin attenuates fasting and postprandial hyperglycemia in animal models of diabetes mellitus. Nutr. Res. Pract. 2011, 5, 107–111.
  17. Bumrungpert, A.; Lilitchan, S.; Tuntipopipat, S.; Tirawanchai, N.; Komindr, S. Ferulic acid supplementation improves lipid profiles, oxidative stress, and inflammatory status in hyperlipidemic subjects: A randomized, double-blind, placebo-controlled clinical trial. Nutrients 2018, 10, 713.
  18. Adeyi, O.E.; Somade, O.T.; Ajayi, B.O.; James, A.S.; Adeboye, T.R.; Olufemi, D.A.; Oyinlola, E.V.; Sanyaolu, E.T.; Mufutau, I.O. The anti-inflammatory effect of ferulic acid is via the modulation of NFκB-TNF-α-IL-6 and STAT1-PIAS1 signaling pathways in 2-methoxyethanol-induced testicular inflammation in rats. Phytomedicine Plus 2023, 3, 100464.
  19. Rai, V.; Jamuna, B.; Joceline, C.; Moreira, S.; Joceline, C.; Moreira, S. Science against microbial pathogens: Communicating current research and technological advances. Microbiol. Ser. 2011, 1, 197.
  20. Vaou, N.; Stavropoulou, E.; Voidarou, C.; Tsigalou, C.; Bezirtzoglou, E. Towards advances in medicinal plant antimicrobial activity: A review study on challenges and future perspectives. Microorganisms 2021, 9, 2041.
  21. Liu, J.; Zhou, H.; Song, L.; Yang, Z.; Qiu, M.; Wang, J.; Shi, S. Anthocyanins: Promising natural products with diverse pharmacological activities. Molecules 2021, 26, 3807.
  22. Krzepiłko, A.; Prażak, R.; Święciło, A. Chemical composition, antioxidant and antimicrobial activity of raspberry, blackberry and raspberry-blackberry hybrid leaf buds. Molecules 2021, 26, 327.
  23. Gil-Martínez, L.; Mut-Salud, N.; Ruiz-García, J.A.; Falcón-Piñeiro, A.; Maijó-Ferré, M.; Baños, A.; De la Torre-Ramírez, J.M.; Guillamón, E.; Verardo, V.; Gómez-Caravaca, A.M. Phytochemicals determination, and antioxidant, antimicrobial, anti-inflammatory and anticancer activities of blackberry fruits. Foods 2023, 12, 1505.
  24. Mihok, E.; György, É.; Máthé, E. The Carpathian lingonberry, raspberry and blackberry fruit extracts feature variable antimicrobial efficiency. Acta Agrar. Debreceniensis 2019, 23, 27–32.
  25. Hajaji, S.; Jabri, M.A.; Sifaoui, I.; López-Arencibia, A.; Reyes-Batlle, M.; B’chir, F.; Valladares, B.; Pinero, J.E.; Lorenzo-Morales, J.; Akkari, H. Amoebicidal, antimicrobial and in vitro ROS scavenging activities of Tunisian Rubus ulmifolius Schott, methanolic extract. Exp. Parasitol. 2017, 183, 224–230.
  26. Chen, H.; Yu, W.; Chen, G.; Meng, S.; Xiang, Z.; He, N. Antinociceptive and antibacterial properties of anthocyanins and flavonols from fruits of black and non-black mulberries. Molecules 2018, 23, 4.
  27. Budiman, A.; Aulifa, D.L.; Kusuma, A.S.W.; Sulastri, A. Antibacterial and antioxidant activity of black mulberry (Morus nigra L.) extract for acne treatment. Pharmacogn. J. 2017, 9, 611–614.
  28. Khalid, N.; Fawad, S.A.; Ahmed, I. Antimicrobial activity, phytochemical profile and trace minerals of black mulberry (Morus nigra L.) fresh juice. Pak. J. Bot. 2011, 43, 91–96.
  29. Wang, S.; Melnyk, J.P.; Tsao, R.; Marcone, M.F. How natural dietary antioxidants in fruits, vegetables and legumes promote vascular health. Food Res. Int. 2011, 44, 14–22.
  30. Silva, L.P.; Pereira, E.; Pires, T.C.S.P.; Alves, M.J.; Pereira, O.R.; Barros, L.; Ferreira, I.C.F.R. Rubus ulmifolius Schott fruits: A detailed study of its nutritional, chemical and bioactive properties. Food Res. Int. 2019, 119, 34–43.
  31. Shahat, A.A.; Cos, P.; De Bruyne, T.; Apers, S.; Hammouda, F.M.; Ismail, S.I.; Azzam, S.; Claeys, M.; Goovaerts, E.; Pieters, L.; et al. Antiviral and antioxidant activity of flavonoids and proanthocyanidins from Crataegus sinaica. Planta Med. 2002, 68, 539–541.
  32. Paredes-López, O.; Cervantes-Ceja, M.L.; Vigna-Pérez, M.; Hernández-Pérez, T. Berries: Improving human health and healthy aging, and promoting quality life- A review. Plant Foods Hum. Nutr. 2010, 65, 299–308.
  33. Youdim, K.A.; Joseph, J.A. A possible emerging role of phytochemicals in improving age-related neurological dysfunctions: A multiplicity of effects. Free Radic. Biol. Med. 2001, 30, 583–594.
  34. Cooper, D.; Doucet, L.; Pratt, M. Influence of cultivar, conventional and organic agricultural practices on phenolic and sensory profile of blackberries (Rubus fruticosus). J. Organ. Behav. 2007, 28, 303–325.
  35. Morales, P.; Ferreira, I.C.F.R.; Carvalho, A.M.; Fernández-Ruiz, V.; Sánchez-Mata, M.S.O.S.C.C.; Cámara, M.; Morales, R.; Tardío, J. Wild edible fruits as a potential source of phytochemicals with capacity to inhibit lipid peroxidation. Eur. J. Lipid Sci. Technol. 2013, 115, 176–185.
  36. Pap, N.; Fidelis, M.; Azevedo, L.; do Carmo, M.A.V.; Wang, D.; Mocan, A.; Pereira, E.P.R.; Xavier-Santos, D.; Sant’Ana, A.S.; Yang, B.; et al. Berry polyphenols and human health: Evidence of antioxidant, anti-inflammatory, microbiota modulation, and cell-protecting effects. Curr. Opin. Food Sci. 2021, 42, 167–186.
  37. Olas, B. Berry phenolic antioxidants—Implications for human health? Front. Pharmacol. 2018, 26, 78.
  38. Nowak, R.; Olech, M.; Nowacka, N. Plant polyphenols as chemopreventive agents. Polyphen. Hum. Health Dis. 2013, 2, 1289–1307.
  39. Wang, S.Y.; Jiao, H. Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxyl radicals, and singlet oxygen. J. Agric. Food Chem. 2000, 48, 5677–5684.
  40. Shih, P.H.; Yeh, C.T.; Yen, G.C. Anthocyanins induce the activation of phase II enzymes through the antioxidant response element pathway against oxidative stress-induced apoptosis. J. Agric. Food Chem. 2007, 55, 9427–9435.
  41. Marulanda, M.L.; López, A.M.; Aguilar, S.B. Genetic diversity of wild and cultivated Rubus species in Colombia using AFLP and SSR markers. Crop Breed. Appl. Biotechnol. 2007, 7, 242–252.
  42. Gonçalves, A.C.; Campos, G.; Alves, G.; Garcia-Viguera, C.; Moreno, D.A.; Silva, L.R. Physical and phytochemical composition of 23 Portuguese sweet cherries as conditioned by variety (or genotype). Food Chem. 2021, 335, 127637.
  43. Skrovankova, S.; Ercisli, S.; Ozkan, G.; Ilhan, G.; Sagbas, H.I.; Karatas, N.; Jurikova, T.; Mlcek, J. Diversity of phytochemical and antioxidant characteristics of black mulberry (Morus nigra L.) fruits from Turkey. Antioxidants 2022, 11, 1339.
  44. Okatan, V. Phenolic compounds and phytochemicals in fruits of black mulberry (Morus nigra L.) genotypes from the Aegean region in Turkey. Folia Hortic. 2018, 30, 93–101.
  45. Vega, E.N.; Molina, A.K.; Pereira, C.; Dias, M.I.; Heleno, S.A.; Rodrigues, P.; Fernandes, I.P.; Barreiro, M.F.; Stojković, D.; Soković, M.; et al. Anthocyanins from Rubus fruticosus L. and Morus nigra L. applied as food colorants: A natural alternative. Plants 2021, 10, 1181.
  46. Mikulic-Petkovsek, M.; Schmitzer, V.; Slatnar, A.; Stampar, F.; Veberic, R. Composition of sugars, organic acids, and total phenolics in 25 wild or cultivated berry species. J. Food Sci. 2012, 77, 1–7.
  47. Castro, R.I.; Vásquez-Rojas, C.; Cortiella, M.G.I.; Parra-Palma, C.; Ramos, P.; Morales-Quintana, L. Evolution of the volatile organic compounds, phenols and antioxidant capacity during fruit ripening and development of Rubus ulmifolius Schott fruits. Horticulturae 2023, 9, 13.
  48. Huang, W.Y.; Zhang, H.C.; Liu, W.X.; Li, C.Y. Survey of antioxidant capacity and phenolic composition of blueberry, blackberry, and strawberry in Nanjing. J. Zhejiang Univ. Sci. B 2012, 13, 94–102.
  49. Wang, R.S.; Dong, P.H.; Shuai, X.X.; Chen, M.S. Evaluation of different black mulberry fruits (Morus nigra L.) based on phenolic compounds and antioxidant activity. Foods 2022, 11, 1252.
  50. Ryu, J.; Kwon, S.-J.; Jo, Y.D.; Jin, C.H.; Nam, B.M.; Lee, S.Y.; Jeong, S.W.; Im, S.B.; Oh, S.C.; Cho, L.; et al. Comparison of phytochemicals and antioxidant activity in blackberry (Rubus fruticosus L.) fruits of mutant lines at the different harvest time. Plant Breed. Biotechnol. 2016, 4, 242–251.
  51. Zafra-Rojas, Q.; Cruz-Cansino, N.; Delgadillo-Ramírez, A.; Alanís-García, E.; Añorve-Morga, J.; Quintero-Lira, A.; Castañeda-Ovando, A.; Ramírez-Moreno, E. Organic acids, antioxidants, and dietary fiber of Mexican blackberry (Rubus fruticosus) residues cv. Tupy. J. Food Qual. 2018, 2018, 5950761.
  52. Croge, C.P.; Cuquel, F.L.; Pintro, P.T.M.; Biasi, L.A.; Bona, C.M. Antioxidant capacity and polyphenolic compounds of blackberries produced in different climates. HortScience 2019, 54, 2209–2213.
  53. Ruiz-Rodríguez, B.M.; Sánchez-Moreno, C.; De Ancos, B.; De Cortes Sánchez-Mata, M.; Fernández-Ruiz, V.; Cámara, M.; Tardío, J. Wild Arbutus unedo L. and Rubus ulmifolius Schott fruits are underutilized sources of valuable bioactive compounds with antioxidant capacity. Fruits 2014, 69, 435–448.
  54. Yiğit, D.; Mavi, A.; Aktaş, M. Antıoxıdant actıvıtıes of black mulberry (Morus nigra). Fen Bilim. Enstitüsü Derg. 2008, 1, 223–232.
  55. Koczka, N.; Stefanovits-Bányai, É.; Prokaj, E. Element composition, total phenolics and antioxidant activity of wild and cultivated blackberry (Rubus fruticosus L.) fruits and leaves during the harvest time. Not. Bot. Horti Agrobot. Cluj-Napoca 2018, 46, 563–569.
  56. Arfan, M.; Khan, R.; Rybarczyk, A.; Amarowicz, R. Antioxidant activity of mulberry fruit extracts. Int. J. Mol. Sci. 2012, 13, 2472–2480.
  57. Bae, S.H.; Suh, H.J. Antioxidant activities of five different mulberry cultivars in Korea. LWT 2007, 40, 955–962.
  58. Li, J.; Shi, C.; Shen, D.; Han, T.; Wu, W.; Lyu, L.; Li, W. Composition and antioxidant activity of anthocyanins and non-anthocyanin flavonoids in blackberry from different growth stages. Foods 2022, 11, 2902.
  59. Feng, R.; Wang, Q.; Tong, W.; Xiong, J.; Wei, Q.; Zhou, W.; Yin, Z.; Jia, R.; Song, X.; Zou, Y.; et al. Extraction and antioxidant activity of flavonoids of Morus nigra. Int. J. Clin. Exp. Med. 2015, 8, 22328–22336.
  60. Miller, D.D.; Li, T.; Liu, R.H. Antioxidants and phytochemicals. Ref. Modul. Biomed. Sci. 2014, 1–13.
  61. Chaves, V.C.; Boff, L.; Vizzotto, M.; Calvete, E.; Reginatto, F.H.; Simões, C.M. Berries grown in Brazil: Anthocyanin profiles and biological properties. J. Sci. Food Agric. 2018, 98, 4331–4338.
  62. Bowen-Forbes, C.S.; Zhang, Y.; Nair, M.G. Anthocyanin content, antioxidant, anti-inflammatory and anticancer properties of blackberry and raspberry fruits. J. Food Compos. Anal. 2010, 23, 554–560.
  63. Rapjut, S.; Wilber, A. Roles of inflammation in cancer initiation, progression, and metastasis. Front. Biosci. (Schol. Ed.) 2010, 2, 810–818.
  64. Lail, H.L.; Feresin, R.G.; Hicks, D.; Stone, B.; Price, E.; Wanders, D. Berries as a treatment for obesity-induced inflammation: Evidence from preclinical models. Nutrients 2021, 13, 334.
  65. Kaume, L.; Gilbert, W.C.; Brownmiller, C.; Howard, L.R.; Devareddy, L. Cyanidin 3-O-β-d-glucoside-rich blackberries modulate hepatic gene expression, and anti-obesity effects in ovariectomized rats. J. Funct. Foods 2012, 4, 480–488.
  66. European Food Safety Authority. Dietary Reference Values for Nutrients—Summary Report. ESFA J. 2017, 14, e15121E.
  67. Slavin, J.L.; Lloyd, B. Health beneficts of fruits and vegetables. Adv. Nutr. 2012, 3, 506–516.
  68. Khoo, H.E.; Azlan, A.; Tang, S.T.; Lim, S.M. Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food Nutr. Res. 2017, 61, 1361779.
  69. Serra, D.; Paixão, J.; Nunes, C.; Dinis, T.C.P.; Almeida, L.M. Cyanidin-3-glucoside suppresses cytokine-induced inflammatory response in human intestinal cells: Comparison with 5-aminosalicylic acid. PLoS ONE 2013, 8, e0073001.
  70. Johnson, M.H.; De Mejia, E.G.; Fan, J.; Lila, M.A.; Yousef, G.G. Anthocyanins and proanthocyanidins from blueberry-blackberry fermented beverages inhibit markers of inflammation in macrophages and carbohydrate-utilizing enzymes in vitro. Mol. Nutr. Food Res. 2013, 57, 1182–1197.
  71. Lee, S.G.; Kim, B.; Yang, Y.; Pham, T.X.; Park, Y.K.; Manatou, J.; Koo, S.I.; Chun, O.K.; Lee, J.Y. Berry anthocyanins suppress the expression and secretion of proinflammatory mediators in macrophages by inhibiting nuclear translocation of NF-κB independent of NRF2-mediated mechanism. J. Nutr. Biochem. 2014, 25, 404–411.
  72. Sangiovanni, E.; Vrhovsek, U.; Rossoni, G.; Colombo, E.; Brunelli, C.; Brembati, L.; Trivulzio, S.; Gasperotti, M.; Mattivi, F.; Bosisio, E.; et al. Ellagitannins from Rubus berries for the control of gastric inflammation: In vitro and in vivo studies. PLoS ONE 2013, 8, e0071762.
  73. González-Sarrías, A.; Larrosa, M.; Toms-Barberán, F.A.; Dolara, P.; Espín, J.C. NF-κB-dependent anti-inflammatory activity of urolithins, gut microbiota ellagic acid-derived metabolites, in human colonic fibroblasts. Br. J. Nutr. 2010, 104, 503–512.
  74. Leyane, T.S.; Jere, S.W.; Houreld, N.N. Oxidative stress in ageing and chronic degenerative pathologies: Molecular mechanisms involved in counteracting oxidative stress and chronic inflammation. Int. J. Mol. Sci. 2022, 23, 7273.
  75. Wang, L.; Wang, X.; Wang, Q. Oxidative stress in neurodegenerative diseases: From molecular mechanisms to clinical applications. Biomed. Res. 2017, 28, 3568–3573.
  76. Tavares, L.; Figueira, I.; MacEdo, D.; McDougall, G.J.; Leitão, M.C.; Vieira, H.L.A.; Stewart, D.; Alves, P.M.; Ferreira, R.B.; Santos, C.N. Neuroprotective effect of blackberry (Rubus sp.) polyphenols is potentiated after simulated gastrointestinal digestion. Food Chem. 2012, 131, 1443–1452.
  77. Kang, T.H.; Hur, J.Y.; Kim, H.B.; Ryu, J.H.; Kim, S.Y. Neuroprotective effects of the cyanidin-3-O-β-D-glucopyranoside isolated from mulberry fruit against cerebral ischemia. Neurosci. Lett. 2006, 391, 122–126.
  78. Tavares, L.; Figueira, I.; McDougall, G.J.; Vieira, H.L.A.; Stewart, D.; Alves, P.M.; Ferreira, R.B.; Santos, C.N. Neuroprotective effects of digested polyphenols from wild blackberry species. Eur. J. Nutr. 2013, 52, 225–236.
  79. Subash, S.; Essa, M.M.; Al-Adawi, S.; Memon, M.A.; Manivasagam, T.; Akbar, M. Neuroprotective effects of berry fruits on neurodegenerative diseases. Neural Regen. Res. 2014, 9, 1557–1566.
  80. Mattioli, R.; Francioso, A.; Mosca, L.; Silva, P. Anthocyanins: A comprehensive review of their chemical properties and health effects on cardiovascular and neurodegenerative diseases. Molecules 2020, 25, 3809.
  81. Taofiq, O.; González-Paramás, A.M.; Barreiro, M.F.; Ferreira, I.C.F.R.; McPhee, D.J. Hydroxycinnamic acids and their derivatives: Cosmeceutical significance, challenges and future perspectives, a review. Molecules 2017, 22, 281.
  82. Ullah, A.; Munir, S.; Badshah, S.L.; Khan, N.; Ghani, L.; Poulson, B.G.; Emwas, A.H.; Jaremko, M. Important flavonoids and their role as a therapeutic agent. Molecules 2020, 25, 5243.
  83. Chen, G.; Bower, K.A.; Xu, M.; Ding, M.; Shi, X.; Ke, Z.J.; Luo, J. Cyanidin-3-glucoside reverses ethanol-induced inhibition of neurite outgrowth: Role of glycogen synthase kinase 3 beta. Neurotox. Res. 2009, 15, 321–331.
  84. Sukprasansap, M.; Chanvorachote, P.; Tencomnao, T. Cyanidin-3-glucoside activates Nrf2-antioxidant response element and protects against glutamate-induced oxidative and endoplasmic reticulum stress in HT22 hippocampal neuronal cells. BMC Complement. Med. Ther. 2020, 20, 1–12.
  85. Fernández-Demeneghi, R.; Rodríguez-Landa, J.F.; Guzmán-Gerónimo, R.I.; Acosta-Mesa, H.G.; Meza-Alvarado, E.; Vargas-Moreno, I.; Herrera-Meza, S. Effect of blackberry juice (Rubus fruticosus L.) on anxiety-like behaviour in Wistar rats. Int. J. Food Sci. Nutr. 2019, 70, 856–867.
  86. Wang, E.; Wang, N.; Zou, Y.; Fahim, M.; Zhou, Y.; Yang, H.; Liu, Y.; Li, H. Black mulberry (Morus nigra) fruit extract alleviated AD-like symptoms induced by toxic Aβ protein in transgenic Caenorhabditis elegans via insulin DAF-16 signaling pathway. Food Res. Int. 2022, 160, 111696.
  87. McGuire, S. World cancer report 2014. Geneva, Switzerland: World Health Organization, International Agency for Research on Cancer, WHO Press, 2015. Adv. Nutr. 2016, 7, 418–419.
  88. Bertoia, M.L.; Mukamal, K.J.; Cahill, L.E.; Hou, T.; Ludwig, D.S.; Mozaffarian, D.; Willett, W.C.; Hu, F.B.; Rimm, E.B. Changes in intake of fruits and vegetables and weight change in United States men and women followed for up to 24 years: Analysis from three prospective cohort studies. PLoS Med. 2015, 12, e1001878.
  89. Donaldson, M.S. Nutrition and cancer: A review of the evidence for an anti-cancer diet. Nutr. J. 2004, 3, 1–21.
  90. Kristo, A.S.; Klimis-Zacas, D.; Sikalidis, A.K. Protective role of dietary berries in cancer. Antioxidants 2016, 5, 37.
  91. Ayvaz, H.; Cabaroglu, T.; Akyildiz, A.; Pala, C.U.; Temizkan, R.; Ağçam, E.; Ayvaz, Z.; Durazzo, A.; Lucarini, M.; Direito, R.; et al. Anthocyanins: Metabolic digestion, bioavailability, therapeutic effects, current pharmaceutical/industrial use, and innovation potential. Antioxidants 2023, 12, 48.
  92. Dharmawansa, K.V.S.; Hoskin, D.W.; Vasantha, H.P.R. Chemopreventive effect of dietary anthocyanins against gastrointestinal cancers: A review of recent advances and perspectives. Int. J. Mol. Sci. 2020, 21, 6555.
  93. Tatar, M.; Bagheri, Z.; Varedi, M.; Naghibalhossaini, F. Blackberry extract inhibits telomerase activity in human colorectal cancer cells. Nutr. Cancer 2019, 71, 461–471.
  94. Feng, R.; Bowman, L.L.; Lu, Y.; Leonard, S.S.; Shi, X.; Jiang, B.H.; Castranova, V.; Vallyathan, V.; Ding, M. Blackberry extracts inhibit activating protein 1 activation and cell transformation by perturbing the mitogenic signaling pathway. Nutr. Cancer 2004, 50, 80–89.
  95. Chen, P.-N.; Chu, S.-C.; Chiou, H.-L.; Yang, S.-F.; Hsieh, Y.-S. Cyanidin 3-glucoside and peonidin 3-glucoside inhibit tumor cell growth and induce apoptosis in vitro and suppress tumor growth in vivo. Nutr. Cancer 2005, 53, 37–41.
  96. Qian, Z.; Wu, Z.; Huang, L.; Qiu, H.; Wang, L.; Li, L.; Yao, L.; Kang, K.; Qu, J.; Wu, Y.; et al. Mulberry fruit prevents LPS-induced NF-ΚB/pERK/MAPK signals in macrophages and suppresses acute colitis and colorectal tumorigenesis in mice. Sci. Rep. 2015, 5, 17348.
  97. Namara, K.M.; Alzubaidi, H.; Jackson, J.K. Cardiovascular disease as a leading cause of death: How are pharmacists getting involved? Integr. Pharm. Res. Pract. 2019, 8, 1–11.
  98. Saini, R.K.; Nile, S.H.; Park, S.W. Carotenoids from fruits and vegetables: Chemistry, analysis, occurrence, bioavailability and biological activities. Food Res. Int. 2015, 73, 735–750.
  99. Han, X.; Shen, T.; Lou, H. Dietary polyphenols and their biological significance. Int. J. Mol. Sci. 2007, 2, 950–988.
  100. Rambaran, T.F.; Nembhard, N.; Bowen-Forbes, C.S.; Alexander-Lindo, R.L. Hypoglycemic effect of the fruit extracts of two varieties of Rubus rosifolius. J. Food Biochem. 2020, 44, 1–11.
  101. Ghorbani, A.; Hooshmand, S. Protective effects of Morus nigra and its phytochemicals against hepatotoxicity: A review of preclinical studies. Pharmacology 2021, 106, 233–243.
  102. Jiang, Y.; Dai, M.; Nie, W.J.; Yang, X.R.; Zeng, X.C. Effects of the ethanol extract of black mulberry (Morus nigra L.) fruit on experimental atherosclerosis in rats. J. Ethnopharmacol. 2017, 200, 228–235.
  103. Freedman, J.E.; Parker, C.; Li, L.; Perlman, J.A.; Frei, B.; Ivanov, V.; Deak, L.R.; Iafrati, M.D.; Folts, J.D. Select flavonoids and whole juice from purple grapes inhibit platelet function and enhance nitric oxide release. Circulation 2001, 103, 2792–2798.
  104. Jimenez-Garcia, S.N.; Guevara-Gonzalez, R.G.; Miranda-Lopez, R.; Feregrino-Perez, A.A.; Torres-Pacheco, I.; Vazquez-Cruz, M.A. Functional properties and quality characteristics of bioactive compounds in berries: Biochemistry, biotechnology, and genomics. Food Res. Int. 2013, 54, 1195–1207.
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Mariana S. Martins
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Ana C. Gonçalves
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Gilberto Alves
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Luís R. Silva
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