Submitted Successfully!
To reward your contribution, here is a gift for you: A free trial for our video production service.
Thank you for your contribution! You can also upload a video entry or images related to this topic.
Version Summary Created by Modification Content Size Created at Operation
1 -- 1070 2023-06-06 05:20:59 |
2 format correct -2 word(s) 1068 2023-06-06 08:58:35 |

Video Upload Options

Do you have a full video?

Confirm

Are you sure to Delete?
Cite
If you have any further questions, please contact Encyclopedia Editorial Office.
Bobrovskikh, M.A.; Gruntenko, N.E. Stress-Related Hormones in Drosophila melanogaster. Encyclopedia. Available online: https://encyclopedia.pub/entry/45215 (accessed on 15 June 2024).
Bobrovskikh MA, Gruntenko NE. Stress-Related Hormones in Drosophila melanogaster. Encyclopedia. Available at: https://encyclopedia.pub/entry/45215. Accessed June 15, 2024.
Bobrovskikh, Margarita A., Nataly E. Gruntenko. "Stress-Related Hormones in Drosophila melanogaster" Encyclopedia, https://encyclopedia.pub/entry/45215 (accessed June 15, 2024).
Bobrovskikh, M.A., & Gruntenko, N.E. (2023, June 06). Stress-Related Hormones in Drosophila melanogaster. In Encyclopedia. https://encyclopedia.pub/entry/45215
Bobrovskikh, Margarita A. and Nataly E. Gruntenko. "Stress-Related Hormones in Drosophila melanogaster." Encyclopedia. Web. 06 June, 2023.
Stress-Related Hormones in Drosophila melanogaster
Edit

The response of living beings to adverse conditions, known as the stress reaction, is a complex mechanism including various signaling pathways and hormones. Some are evolutionarily conserved, such as the insulin signaling pathway, others, such as 20-hydroxyecdysone, adipokinetic or juvenile hormones, are taxon-specific in insects. Key components of the neuroendocrine stress reaction in insects are biogenic amines (dopamine and octopamine), juvenile hormone, 20-hydroxyecdysone, adipokinetic hormone and insulin-like peptides. 

Drosophila melanogaster insulin signaling pathway juvenile hormone 20-hydroxyecdysone adipokinetic hormone dopamine octopamine stress

1. Introduction

Adverse environmental effects on living beings launch a series of reactions on the cellular, neuroendocrine and behavioral levels, which leads to the activation of defense processes and enhances adaptation. In insects, the neuroendocrine stress reaction is currently considered to include the following elements: the insulin signaling pathway, biogenic amines, dopamine and octopamine, functioning as both neuromediators and neurohormones, the neuropeptide adipokinetic hormone, as well as 20-hydroxyecdysone and the juvenile hormone–two hormones controlling larvae development, metamorphosis and reproduction. Disruption of any of the components of the neuroendocrine stress reaction can influence insect stress resistance.

2. Insulin/Insulin-Like Growth Factors Signaling Pathway in Drosophila melanogaster

The insulin/insulin-like growth factors signaling (IIS) pathway is evolutionarily conserved among all metazoans and performs a vital role in the regulation of growth, development, reproduction, longevity, metabolism and stress resistance [1][2]. In D. melanogaster, eight insulin-like peptides (DILP1-8) have been identified: DILP1-5 show significant homology with insulin, DILP6–with insulin-like growth factors, DILP7 and DILP8–with mammalian relaxins [3][4][5][6][7].
DILPs are produced in medial neurosecretory cells or insulin-producing cells (IPCs) of the brain, as well as in the cells of peripheral tissues such as the visceral muscles of the gut, the fat body, which is the main metabolic organ in insects, neurons of the abdominal ganglia, and ovaries in a tissue- and stage-specific way [3][6][8][9][10][11][12]. Neuronal DILPs are secreted into the hemolymph and received by a homolog of the insulin receptor (dInR) for transmitting its signals to target cells [8][13][14][15][16]. DILP7 acts on the Lgr4 receptor bound to G-protein and containing leucine-rich repeats, and DILP8 binds Lgr3 [17][18].
dInR is localized in numerous fly tissues, including the fat body, the endocrine gland corpus allatum (CA) and follicular cells of the ovaries [19][20][21]. Activation of dInR, directly or through an orthologue of the mammalian insulin receptor substrate (CHICO), launches the kinase cascade, and dAkt/PKB (proteinkinase B homolog) inhibits the transfer of the transcription factor of the Drosophila Forkhead box class O family (dFOXO) into the cell nucleus and provokes its return from the nucleus back to the cytoplasm [22]. The main localization of dFOXO in D. melanogaster is the fat body of the head and abdomen [23]. dFOXO plays the role of the main regulator of expression of the downstream genes participating in the metabolism, the cell cycle, the stress response, the control of longevity and apoptosis [24][25][26][27]. It has been shown that a mutant dFOXO lacking dAkt phosphorylation sites does not react to IIS inhibition, remains in the nucleus and is constitutively active [28].

3. Stress-Related Hormones in Drosophila melanogaster

In D. melanogaster, IPCs are similar to vertebrate pancreatic β-cells secreting insulin in response to hyperglycemia, and the role of pancreatic α-cells secreting glucagon in response to hypoglycemia is performed by the cells of the corpus cardiacum (CC) gland, which produce a glucagon-like neuropeptide, the adipokinetic hormone (AKH) [3][29][30][31]. Due to the similarity of their functions to those of α- and β-cells, IPCs and CC, taken together, are seen as the Drosophila analogue of the mammalian pancreatic gland [32].
AKH regulates metabolic response to stress, stimulating catabolic reactions and mobilizing energy stores, especially lipids and trehalose, the latter being the main carbohydrate in insects [33][34]. It has been discovered that AKH deficit leads to obesity and a decrease in the carbohydrate level in D. melanogaster imagoes [35][36], and flies with an Akh mutation have much lower carbohydrate levels in hemolymph, including trehalose, and are resistant to starvation [34]. It has also been shown that Akh expression and AKH content in the cells are under DILP2 regulation in D. melanogaster females [11].
The IIS pathway interacts with other key hormones of the insect neuroendocrine stress reaction: 20-hydroxyecdysone (20E) and juvenile hormone (JH), which play a decisive role in growth, development, molting and metamorphosis in larvae, and perform the function of gonadotropins in imagoes, as well as biogenic amines dopamine (DA) and octopamine (OA) [22].
The central location of the OA and DA synthesis is octopamine- and dopaminergic neurons of the brain, the location of the JH synthesis is the CA gland [37][38]. It has been established that ecdysteroid biosynthesis during development takes place in the prothoracic gland, and the ovaries serve as the main source of ecdysteroids in imagoes [21][37][39]; seminal glands also contain ecdysteroids, but there is no sufficient evidence of an entire de novo biosynthesis pathway in this tissue [39]. In insects, DA and OA play the role of neurotransmitters in the synaptic cleft, neuromodulators within the bounds of one tissue, and neurohormones when being transmitted by the flow of the hemolymph to large distances [40]. It has been shown that they control the activity of the endocrine glands, arousal, desensitization of sensory inputs, various complex behavior forms such as memory and learning, and mobilization of lipids and carbohydrates [40][41][42].
DA is known to participate in JH level regulation, increasing it in young females, and decreasing it in mature ones [43]. Moreover, its regulation has a feedback loop: JH lowers the DA level in young females and increases it in mature ones. DA also regulates the 20E level, increasing it in young females and decreasing it in mature ones; however, no negative relationship has been discovered–20E increases the DA level in young females and decreases it in mature ones. In turn, DA and OA influence the 20E level indirectly through the JH metabolic system. This influence is unidirectional in young females, where it increases the 20E level, and multidirectional in mature ones: OA increases the 20E level, and DA decreases it [43]. Under unfavorable conditions of varying nature, the levels of all these hormones in Drosophila imagoes increase sharply (accompanied by a decrease in the activity of their metabolic enzymes), affecting survival, fecundity and longevity [22]. In larvae, stress reaction develops as an inhibition of prothoracicotropic hormone (PTTH) secretion, which leads to a delay in ecdysone secretion and an increase in JH content, resulting in delayed metamorphosis or additional molting and allowing to “wait out” unfavorable conditions [44]. OA and DA levels in larval insects have also been shown to increase under heat stress [44][45].

References

  1. Garofalo, R.S. 2 Genetic analysis of insulin signaling in Drosophila. Trends Endocrinol. Metab. 2002, 13, 156–162.
  2. Chowański, S.; Walkowiak-Nowicka, K.; Winkiel, M.; Marciniak, P.; Urbański, A.; Pacholska-Bogalska, J. Insulin-like peptides and cross-talk with other factors in the regulation of insect metabolism. Front. Physiol. 2021, 12, 701203.
  3. Brogiolo, W.; Stocker, H.; Ikeya, T.; Rintelen, F.; Fernandez, R.; Hafen, E. An evolutionarily conserved function of the Drosophila insulin receptor and insulin-like peptides in growth control. Curr. Biol. 2001, 11, 213–221.
  4. Grönke, S.; Clarke, D.F.; Broughton, S.; Andrews, T.D.; Partridge, L. Molecular evolution and functional characterization of Drosophila insulin-like peptides. PLoS Genet. 2010, 6, e1000857.
  5. Garelli, A.; Gontijo, A.M.; Miguela, V.; Caparros, E.; Dominguez, M. Imaginal discs secrete insulin-like peptide 8 to mediate plasticity of growth and maturation. Science 2012, 336, 579–582.
  6. Colombani, J.; Andersen, D.S.; Leopold, P. Secreted peptide Dilp8 coordinates Drosophila tissue growth with developmental timing. Science 2012, 336, 582–585.
  7. Álvarez-Rendón, J.P.; Salceda, R.; Riesgo-Escovar, J.R. Drosophila melanogaster as a model for diabetes type 2 progression. BioMed Res. Int. 2018, 2018, 1417528.
  8. Miguel-Aliaga, I.; Thor, S.; Gould, A.P. Postmitotic specification of Drosophila insulinergic neurons from pioneer neurons. PLoS Biol. 2008, 6, e58.
  9. Bai, H.; Kang, P.; Tatar, M. Drosophila insulin-like peptide-6 (dilp6) expression from fat body extends lifespan and represses secretion of Drosophila insulin-like peptide-2 from the brain. Aging Cell 2012, 11, 978–985.
  10. Post, S.; Karashchuk, G.; Wade, J.D.; Sajid, W.; De Meyts, P.; Tatar, M. Drosophila insulin-like peptides DILP2 and DILP5 differentially stimulate cell signaling and glycogen phosphorylase to regulate longevity. Front. Endocrinol. 2018, 9, 245.
  11. Post, S.; Liao, S.; Yamamoto, R.; Veenstra, J.A.; Nässel, D.R.; Tatar, M. Drosophila insulin-like peptide dilp1 increases lifespan and glucagon-like Akh expression epistatic to dilp2. Aging Cell 2019, 18, 12863.
  12. Prince, E.; Kretzschmar, J.; Trautenberg, L.C.; Broschk, S.; Brankatschk, M. DIlp7-producing neurons regulate insulin-producing cells in Drosophila. Front Physiol. 2021, 12, 630390.
  13. Rulifson, E.J.; Kim, S.K.; Nusse, R. Ablation of insulinproducing neurons in flies: Growth and diabetic phenotypes. Science 2002, 296, 1118–1120.
  14. Géminard, C.; Rulifson, E.J.; Léopold, P. Remote control of insulin secretion by fat cells in Drosophila. Cell Metab. 2009, 10, 199–207.
  15. Cognigni, P.; Bailey, A.P.; Miguel-Aliaga, I. Enteric neurons and systemic signals couple nutritional and reproductive status with intestinal homeostasis. Cell Metab. 2011, 13, 92–104.
  16. Nässel, D.R.; Kubrak, O.I.; Liu, Y.; Luo, J.; Lushchak, O.V. Factors that regulate insulin producing cells and their output in Drosophila. Front. Physiol. 2013, 4, 252.
  17. Gontijo, A.M.; Garelli, A. The biology and evolution of the Dilp8-Lgr3 pathway: A relaxin-like pathway coupling tissue growth and developmental timing control. Mech. Dev. 2018, 154, 44–50.
  18. Imambocus, B.N.; Zhou, F.; Formozov, A.; Wittich, A.; Tenedini, F.M.; Hu, C.; Sauter, K.; Varela, E.M.; Herédia, F.; Casimiro, A.P.; et al. A neuropeptidergic circuit gates selective escape behavior of Drosophila larvae. Curr. Biol. 2022, 32, 149–163.
  19. Belgacem, Y.H.; Martin, J.R. Hmgcr in the corpus allatum controls sexual dimorphism of locomotor activity and body size via the insulin pathway in Drosophila. PLoS ONE 2007, 2, e187.
  20. Rauschenbach, I.Y.; Karpova, E.K.; Adonyeva, N.V.; Andreenkova, O.V.; Faddeeva, N.V.; Burdina, E.V.; Alekseev, A.A.; Menshanov, P.N.; Gruntenko, N.E. Disruption of insulin signalling affects the neuroendocrine stress reaction in Drosophila females. J. Exp. Biol. 2014, 217, 3733–3741.
  21. Andreenkova, O.V.; Adonyeva, N.V.; Eremina, M.A.; Gruntenko, N.E.; Rauschenbach, I.Y. The Insulin-like receptor gene expression in the tissues synthesizing gonadotropic hormones at sexual maturation of Drosophila melanogaster females. Russ. J. Genet. 2016, 52, 1342–1344.
  22. Gruntenko, N.E.; Rauschenbach, I.Y. The role of insulin signalling in the endocrine stress response in Drosophila melanogaster: A mini-review. Gen. Comp. Endocrinol. 2018, 258, 134–139.
  23. Zheng, X.; Yang, Z.; Yue, Z.; Alvarez, J.D.; Sehgal, A. FOXO and insulin signaling regulate sensitivity of the circadian clock to oxidative stress. Proc. Natl. Acad. Sci. USA 2007, 104, 15899–15904.
  24. Puig, O.; Matilla, J. Understanding Forkhead box class O function: Lessons from Drosophila melanogaster. Antioxid. Redox Signal. 2011, 14, 635–647.
  25. Wang, Z.; Yu, T.; Huang, P. Post-translational modifications of FOXO family proteins (Review). Mol. Med. Rep. 2016, 14, 4931–4941.
  26. Gruntenko, N.E.; Adonyeva, N.V.; Burdina, E.V.; Karpova, E.K.; Andreenkova, O.V.; Gladkikh, D.V.; Ilinsky, Y.Y.; Rauschenbach, I.Y. The impact of FOXO on dopamine and octopamine metabolism in Drosophila under normal and heat stress conditions. Biol. Open. 2016, 5, 1706–1711.
  27. Ding, K.; Barretto, E.C.; Johnston, M.; Lee, B.; Gallo, M.; Grewal, S.S. Transcriptome analysis of FOXO-dependent hypoxia gene expression identifies Hipk as a regulator of low oxygen tolerance in Drosophila. G3-Genes Genom. Genet. 2022, 12, jkac263.
  28. Puig, O.; Marr, M.T.; Ruhf, M.L.; Tjian, R. Control of cell number by Drosophila FOXO: Downstream and feedback regulation of the insulin receptor pathway. Genes Dev. 2003, 17, 2006–2020.
  29. Ikeya, T.; Galic, M.; Belawat, P.; Nairz, K.; Hafen, E. Nutrient dependent expression of insulin-like peptides from neuroendocrine cells in the CNS contributes to growth regulation in Drosophila. Curr. Biol. 2002, 12, 1293–1300.
  30. Kim, S.K.; Rulifson, E.J. Conserved mechanisms of glucose sensing and regulation by Drosophila corpora cardiaca cells. Nature 2004, 431, 316–320.
  31. Lee, G.; Park, J.H. Hemolymph sugar homeostasis and starvation-induced hyperactivity affected by genetic manipulations of the adipokinetic hormone-encoding gene in Drosophila melanogaster. Genetics 2004, 167, 311–323.
  32. Hughson, B.N. The glucagon-like adipokinetic hormone in Drosophila melanogaster—Biosynthesis and secretion. Front. Physiol. 2021, 12, 710652.
  33. Isabel, G.; Martin, J.-R.; Chidami, S.; Veenstra, J.A.; Rosay, P. AKH-producing neuroendocrine cell ablation decreases trehalose and induces behavioral changes in Drosophila. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2005, 288, 531–538.
  34. Sajwan, S.; Sidorov, R.; Stašková, T.; Žaloudíková, A.; Takasu, Y.; Kodrík, D.; Zurovec, M. Targeted mutagenesis and functional analysis of adipokinetic hormone-encoding gene in Drosophila. Insect Biochem. Mol. Biol. 2015, 61, 79–86.
  35. Gáliková, M.; Diesner, M.; Klepsatel, P.; Hehlert, P.; Xu, Y.; Bickmeyer, I.; Predel, R.; Kühnlein, R.P. Energy homeostasis control in Drosophila adipokinetic hormone mutants. Genetics 2015, 201, 665–683.
  36. Bednářová, A.; Tomčala, A.; Mochanová, M.; Kodrík, D.; Krishnan, N. Disruption of adipokinetic hormone mediated energy homeostasis has subtle effects on physiology, behavior and lipid status during aging in Drosophila. Front. Physiol. 2018, 9, 949.
  37. Toivonen, J.M.; Partridge, L. Endocrine regulation of aging and reproduction in Drosophila. Mol. Cell Endocrinol. 2009, 299, 39–50.
  38. Zhang, X.; Li, S.; Liu, S. Juvenile hormone studies in Drosophila melanogaster. Front. Physiol. 2022, 12, 785320.
  39. Lafont, R.; Dauphin-Villemant, C.; Warren, J.T.; Rees, H.H. Ecdysteroid chemistry and biochemistry. Ref. Mod. Life Sci. 2017, 3, 125–195.
  40. Sasaki, K.; Harano, K.-I. Multiple regulatory roles of dopamine in behavior and reproduction of social insects. Trends Entomol. 2010, 6, 1–13.
  41. Farooqui, T. Octopamine-mediated neuromodulation of insect senses. Neurochem. Res. 2007, 32, 1511–1529.
  42. Martin, C.A.; Krantz, D.E. Drosophila melanogaster as a genetic model system to study neurotransmitter transporters. Neurochem. Int. 2014, 73, 71–88.
  43. Gruntenko, N.E.; Rauschenbach, I.Y. Interplay of JH, 20E and biogenic amines under normal and stress conditions and its effects on reproduction. J. Insect Physiol. 2008, 54, 902–908.
  44. Rauschenbach, I.Y. Stress response in insects: Mechanism, genetic control, and role in adaptation. Russ. J. Genet. 1997, 33, 942–949.
  45. Hirashima, A.; Nagano, T.; Eto, M. Stress-induced changes in the biogenic amine levels and larval growth of Tribolium castaneum Herbst. Biosci. Biotech. Biochem. 1993, 57, 2085–2089.
More
Information
Subjects: Entomology
Contributors MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register : ,
View Times: 260
Revisions: 2 times (View History)
Update Date: 06 Jun 2023
1000/1000
Video Production Service