Submitted Successfully!
To reward your contribution, here is a gift for you: A free trial for our video production service.
Thank you for your contribution! You can also upload a video entry or images related to this topic.
Version Summary Created by Modification Content Size Created at Operation
1 -- 3369 2023-03-08 09:58:05 |
2 format correct Meta information modification 3369 2023-03-09 01:59:58 |

Video Upload Options

Do you have a full video?

Confirm

Are you sure to Delete?
Cite
If you have any further questions, please contact Encyclopedia Editorial Office.
Nezami, E.; Gallego, P.P. Pistachio Germplasm Propagation and Conservation. Encyclopedia. Available online: https://encyclopedia.pub/entry/41975 (accessed on 17 June 2024).
Nezami E, Gallego PP. Pistachio Germplasm Propagation and Conservation. Encyclopedia. Available at: https://encyclopedia.pub/entry/41975. Accessed June 17, 2024.
Nezami, Esmaeil, Pedro P. Gallego. "Pistachio Germplasm Propagation and Conservation" Encyclopedia, https://encyclopedia.pub/entry/41975 (accessed June 17, 2024).
Nezami, E., & Gallego, P.P. (2023, March 08). Pistachio Germplasm Propagation and Conservation. In Encyclopedia. https://encyclopedia.pub/entry/41975
Nezami, Esmaeil and Pedro P. Gallego. "Pistachio Germplasm Propagation and Conservation." Encyclopedia. Web. 08 March, 2023.
Pistachio Germplasm Propagation and Conservation
Edit

The word “pstk” [pistag], used in the ancient Persian language, is the linguistic root from which the current name “pistachio”, used worldwide, derives. The word pistachio is generally used to designate the plants and fruits of a single species: Pistacia vera L. Both the plant and its fruits have been used by mankind for thousands of years, specifically the consumption of its fruits by Neanderthals has been dated to about 300,000 years ago. Historically, Pistacia spp. germplasms were mainly conserved via conventional macropropagation techniques using in situ (in-site in their native place) habitats or even old orchards, and ex situ (off-site) where material is taken away from their native place to germplasm, botanic gardens, and so on. The strategy is to identify superior genotypes and transfer them to collections as well as to maintain them in the wild. Unconventional biological techniques, including cryopreservation (for the long-term), slow-growth storage conditions and synthetic seeds (for medium-term) and micropropagation (for short-term) have opened new insights for preservation of commercial and endangered Pistacia species. It should be noted that although pistachio species are not globally endangered, at least 12 species are currently included in the IUCN Red List of Threatened Species: P. cucphuongensis (VU, vulnerable), P. vera, P. aethiopica, P. mexicana, P. atlantica (NT, near threatened) and the rest are LN (least concern), so it has been imperative to apply preservation policies to these species.

Pistacia vera plant tissue culture rootstocks

1. Conventional Sexual Propagation

Pistachio reproduces naturally by cross-pollination, so the seeds produced will be as genetically variable as wild genetic populations. It is believed that the first plantings of pistachios in Iran, Turkey, Syria and most of other countries in their native region, were started from selecting and germinating the best seeds of wild Pistacia spp. [1]. However, the use of the seed method for pistachio propagations is not really suitable for commercially establishment of pistachio orchards, because nuts of wild Pistacia spp. are so sensitive to desiccation, as shown by the low germination rates [2]. Additionally, the resulting trees from seedlings are expected to be genetically variable, although they appear to be quite uniform in the field [3]. Most of the old commercial pistachio orchards currently in use in Iran, Turkey and Syria are the result of two forms of genetic selection: (i) selecting wild pistachios of Pistacia sp. and their hybrids capable of bearing fruit in only 5–7 years (normally taking 20–25 years), for hundreds of years, and (ii) using conventional asexual propagation systems, such as grafting selected pistachio tree scions onto rootstocks which are also selected for their agronomic characteristics. This has led to the establishment of orchards in a much shorter time [4][5].

2. Conventional Asexual Propagation: Cuttings and Grafting

Domestication of trees using sexual reproduction, by seeds, present some bottlenecks which naturally delay the process because of the need for two compatible parental trees, in the case of dioecious plants as pistachio, or good conditions for wind-pollination, a long period of time before obtaining the next generation of mature adult tree capable of bearing fruit and, finally, propagation through seed germination is not suitable for establishment of clonally elite orchards since seedlings are not true-to-type trees. Alternatively, asexual or vegetative propagation has been used to improve tolerance/resistance to biotic and abiotic stresses and to accelerate pistachio domestication [6]. Among others, cutting [7] and grafting [8] methods for macropropagation of pistachios have been traditionally used [1][9].

2.1. Propagation by Cuttings

Cuttings are applied to all those woody species in which rooting is not very difficult. In this sense, a range of factors have been reported to impact on the rooting percentage of cuttings from woody species in nurseries: genotype, position on the shoot from which the cutting is made, auxin type and concentration, date of shoot collection and so on, which have been reviewed elsewhere in detail [10][11]. In Pistacia spp. cuttings were traditionally used for the clonal propagation of selected rootstocks in order to obtain uniform progeny [12] (and rarely on cultivars [13]). However, attempts to propagate pistachio rootstocks commercially by soft and hard wood cuttings especially using adult materials have given inconsistent results [13][14].
Pioneering studies on pistachio propagation has shown that the use of pistachio hardwood cuttings, obtained from adult trees, have exhibited a very low rooting rate (about 5%), which has been attributed to the fact that adult trees lose their rooting capacity with age [15][16]. In fact, the first experiments carried out with P. vera and two clonal rootstocks P. palestina and P. atlantica rooted with IBA at 10 mg L−1 was completely unsuccessful [17]. In the 1980s, softwood, instead of hardwood, cuttings of young (one-year-old) P. chinensis trees supplied with the rooting phytohormone Indole butyric Acid (IBA) at 5 mg L−1 resulted in 92% rooting [18]. In addition, high rooting percentages (78–100%) were obtained from P. vera after dipping the softwood cuttings from seedlings into concentrated 500 and 1000 mg L−1 IBA, respectively. Moreover, promising high rooting percentages (88%) of softwood cuttings were obtained using a mist system combined with treatments with IBA at 35 mg L−1 [13]. However, only a 50% rooting was achieved if older plant material (four-year-old) was used [19]. Finally, Almehdi and co-workers documented the significant effects of genotype, part of the shoot from which the cutting was taken and shoot collection data on successful rooting percentage (40%) of the rootstock ‘UCB1’ cuttings, but no effect of IBA concentration and shoot lengths was found [20].

2.2. Propagation by Grafting

Grafting has been used in pistachio domestication as far as 3000–4000 years ago [21][22], being the decisive horticultural technique in the spread of pistachio cultivation from Central Asia to the countries of the Mediterranean basin between 1000 B.C. and 1000 A.D. [23][24]. From a biological point of view, grafting can be defined as the fusion into a single organism of a portion of tissue from one plant with a portion of tissue from another plant, which can occur naturally and artificially [25]. From a horticultural point of view, it is a technique of cloning by vegetative (asexual) artificial propagation of plants in which a portion of tissue (called scion) from a selected cultivar (donor) is attached to another (called stock or rootstock) selected plant for its resistance to biotic and abiotic stresses and serves as the root system of the grafted plant, so that both grow as a single and new organism. Finally, from a genetic point of view, grafting implies the formation of a new organism through the fusion of at least two genotypes, each one maintaining its own genetic identity in the new grafted plant [24].
Grafting has been used in the last 3500 years for various biological and horticultural purposes such as vegetative macropropagation, juvenility avoidance, cultivar change, to repair established adult trees, size-controlling grafted plants, physiological studies, biotic and abiotic stress resistances or identification of asymptomatic viruses [8][26][27][28]. In the case of pistachio, it was traditionally used for vegetative macropropagation and genetic improvement using P. vera plants as a scion and wild Pistacia spp. as rootstocks, as described above.

2.3. Propagation by Budding

Budding is a special type of grafting, in which a small piece of shoot with a single vegetative bud is cut from the wood of the scion and transferred to the rootstock. To improve the efficiency of budding in pistachio species, a number of factors must be taken into account: the selection of a healthy, active rootstock in optimum condition, with a diameter large enough to adequately accommodate the grafted buds, the quality of the scion wood, the use of the most practical technique for budding (e.g., T-budding in the case of pistachio), compatibility between scion and rootstock, the budding season, and proper aftercare [7][29][30][31].
In general, although several improvements based on those traditional techniques of conventional asexual propagation have been reported, low rooting efficiency, lack of producing uniform plants with a high quality at sufficiently large scales were the most important challenges of pistachio nurseries [30][32], and new strategies for conservation using novel unconventional propagation methods based in plant tissue culture were developed for pistachio.

3. Unconventional Asexual Propagation

3.1. Cryopreservation

Cryopreservation can be classified as a long-term conservation technique based on the methods of cooling and storing biological material at very low temperatures in liquid nitrogen for an unlimited time. Specifically, plant cryopreservation refers to the storage of any plant biological material (cells, tissues, or organs) such as seeds, pollen, shoot tips or dormant buds at an ultra-low temperature in order to decrease by a state of non-division and zero metabolism without any genetic alteration or modification in the plant material for an unlimited period of time [33][34][35]. Recently, Sharrock stated the cryopreservation technique as a basic conservation tool for germplasm conservation in the last Global Strategy for Plant Conservation Report [36]. General procedures for the cryopreservation technique include [33][37]: (1) cold hardening (4 °C generally), (2) pre-culture with suitable cryoprotectant (such as sucrose), (3) physical dehydration, over silica gel, or chemical dehydration by using highly concentrated plant vitrification solutions (i.e., PVS2), (4) freezing by immersion in liquid nitrogen (−196 °C), (5) rapid thawing at 35–40 °C and (6) washing and re-culture into fresh medium.
In the case of cryopreservation of Pistacia germplasm, the former technique was developed successfully for seeds of P. vera, P. lentiscus and P. terebinthus [38] and embryonic axes of P. vera [39]; whilst the latter technique was utilized for cryopreservation of the in vitro cultured axillary buds of P. vera [40] and [41]. Despite this, problems related to the low efficiency of recovery of cryopreserved plant materials makes the application of long-term conservation for Pistacia germplasm conservation costly [42]. Thus, much research remains to be conducted in order to design effective cryopreservation protocols that can be used for a wide range of Pistacia species in a more efficient and less costly manner.

3.2. Slow-Growth Storage

Slow-growth storage can be classified as a medium-term conservation technique and consists of maintaining in vitro plant cultures under limited growth conditions, allowing subculture intervals to be extended up to a few months or a few years, without affecting their potential for survival and regeneration after storage [43]. Slow growth protocols require modification of the usual in vitro culture conditions by various means, such as reducing the incubation temperature (but always keeping it above 0 °C) [44], light intensity or even eliminating it completely [45], nutrient concentration in the culture medium and/or supplementation of the culture medium with different osmotic regulators, commonly sugars (sucrose) or alcohols (mannitol or sorbitol) [46] and/or growth retardants (abscisic acid) to minimize the growth of in vitro conserved plants [47][48].
Barghchi, in 1986, pioneered the development of an efficient protocol to increase subcultures interval in pistachio to 18 months by reducing the growth chamber temperature from 26 °C to 4 °C, applying readjustments in photoperiods and light intensity, supplementing the basal culture media with growth retardants such as the phytohormone abscisic acid or the osmotic mannitol at different concentrations [49].
More recently, Kocand co-workers induced cold storage by reducing the temperature of incubation to 4 °C in dark for 2–12 months. They found that during the first 6 months the pistachio plantlets remained green and healthy but after that the occurrence of shoot tip necrosis and death of the shoots in the cold stored plants was found; after 6 months the cold stored plantlets showed more genetic instability than the controls [50]. The studies conducted on slow-growth storage for many plant species are still very limited [51], including Pistacia spp. [40], due to almost all species requiring an appropriate conservation strategy.

3.3. Synthetic Seed Techniques

Another unconventional asexual propagation technique considered as medium-term conservation technique is called synthetic seeds or encapsulation, which is a biotechnological tool based on plant tissue culture that has been widely used to propagate, conserve and deliver germplasm of many economically important species [52][53][54]. It consists of artificially constructing synthetic seeds by encapsulating any somatic plant cells, tissue or organs (i.e., somatic embryos, apical or axillary buds, nodal segments, etc.) in gel capsules, forming spherical beads, for conservation [55]. The synthetic seed technology has led to encapsulated, storage and re-growth clonal plant material (alive somatic explants) allowing the possibility of mass production of elite plant species, and even the automatization of this process. Moreover, synthetic seeds show several advantages with respect to conventional propagation systems compared to seeds, cuttings or grafting, because the encapsulated explants upon germination behave like seeds and possess the ability to convert into plantlets under either in vitro or in vivo conditions [56][57]. Although synthetic seeds are a very promising technology, the protocol for the processing of synthetic seeds present several limitations: limited control of somatic embryogenic genesis, developing and maturation and, once encapsulated, the control of seed germination and production of viable explants to be used as plant material. All of these factors need further optimization [58].
In pistachio, the first synthetic seeds were developed using somatic embryos and embryogenic masses in 1996 by Onay and colleagues [59]. Later, pistachio axillary buds were excised from juvenile seeds [41] and shoot tips apices were also encapsulated [40]. Akdemir and co-workers [40] reported that encapsulated shoot apices of P. vera cultivars were conserved for up to 12 months at 4 °C in dark conditions with the subsequent recovery of over 90%.

3.4. Micropropagation

The conservation of Pistacia spp. germplasm by conventional macropropagation methods has some limitations, such as low rooting efficiency, lack of production of high-quality uniform plants at sufficiently large scales, poor seed viability (after conventional storage), high risk of disease spread, and loss of genetic germplasm, as mentioned above and elsewhere [44][60]. To overcome those limitations, in the last 35 years, several unconventional asexual plant propagation methods based on plant biotechnological advances have been applied for pistachio propagation, including the development of in vitro propagation techniques such as organogenesis [61], somatic embryogenesis [62] and micrografting [19][63][64][65]. These have been widely used for mass propagation and germplasm conservation of Pistacia species. There are many good reviews about the principles of in vitro culture techniques; here, only very general definitions and principles, and physiological problems related to micropropagation are summarized.
Micropropagation is an in vitro plant tissue culture technique used to multiply a large number of identical selected plants (clones) from a plant stock in a very short time, at a competitive price and with high survival rates. Micropropagation is also known as true-to-type in vitro propagation. It has been used for multiple purposes, such as providing quality plants in large quantities (mass propagation) to meet horticulture, silviculture, genetic conservation and industrial (food, pharmaceutical, nutraceutical and cosmeceutical) needs [66]. Micropropagation has many advantages over conventional propagation systems; among others, the possibility of a massive multiplication of elite genotypes in a very short time, the rescue of those genotypes in danger of extinction and those others difficult to propagate by conventional techniques; all these advantages are easily applicable to the Pistacia genus, as described below [67]. The success or failure of micropropagation methods, regardless of whether they are initiated from apical or axillary meristems, adventitious shoots or somatic embryos, depends critically on the effect of a large number of factors, including genotype, culture conditions and, above all, the composition of the culture medium (mineral nutrients of the culture medium, PGRs and vitamins) [68][69][70][71].
In Pistacia spp. most of the micropropagation studies have used Murashige and Skoog (MS) [72] as a basal culture medium for the cultivar P. vera; [73][74][75][76][77][78] and for several rootstocks such as P. khinjuk [2][79][80] P. atlantica [19][79], P. lentiscus [81], and, less extensively, other basal culture media designed for woody plants such as DKW [82] for P. vera [77] or WPM [83] for P. vera [61][63][84][85][86]. However, micropropagation of Pistacia sp. has been hampered by a low multiplication rate in addition to some physiological disorders such as basal callus (BC), shoot tip necrosis (STN), hyperhydricity (H), Leaf yellowing (LY), or vascular necrosis (VN), when these three basal culture media have been used [61][85][87][88]. In addition, some common problems to micropropagation such as culture browning (CB), caused by phenolic compounds secretions, and contamination (CO) were detected in pistachio micropropagation. Table 1 summarizes the main in vitro studies carried out on P. vera L. cultivars and on the different rootstocks, describing the main problems and common physiological disorders observed and the solutions applied to alleviate them; however, those readers interested in knowing the specific solution to each problem and disorder can consult them in detail in each of the referenced studies.
Table 1. Main studies on Pistacia spp. micropropagation indicating basal culture media employed, physiological disorders such as basal callus (BC), shoot tip necrosis (STN), hyperhydricity (H), Leaf yellowing (LY), and vascular necrosis (VN) and some problems such as culture browning (CB) and contamination (CO), and the solutions proposed to alleviate them.
Recent studies on pistachio in vitro propagation growth response to mineral nutrients of culture media have revealed the key roles of some ion interactions (SO42− × Cl, K+ × SO42− × EDTA, and Fe2+ × Cu2+ × NO3) for shoot quality, proliferation rate, and shoot length, whilst physiological disorders (BC and STN) were found to have been significantly impacted by independent ions as Fe2+ and EDTA, respectively [95]. To alleviate STN of P. vera L., some authors have recommended an increase in both the MS boron concentration (up to 100–1000 µM) and calcium concentration (15 mM calcium gluconate [85] or 12–24 mM CaCl2·2H2O [88]). On the other hand, depletion of MS NH4NO3 and CaCl2 (10.33 mM and 1 mM, respectively) and enhancing FeSO4·7H2O and Na2EDTA·2H2O (both 0.15 mM) has been recommended to control STN and LY (chlorosis) in P. vera cv. ‘mateur’ [86].
Early studies on the effect of up to 48 µM AgNO3 have demonstrated improved shoot regeneration and growth and strong anti-browning effect on culture media and explants in some Pistacia species [91][97]. However later on, media supplemented with AgNO3 at 24 µM caused some deteriorative effects on the proliferation rate of P. vera rootstock, although BC was prevented. The most recent studies have revealed the negative effect on some growth parameters of NO3, Mg2+, Ag+ and gluconate at high concentrations [98].
To improve pistachio shoot multiplication, standard culture media vitamins have been replaced with other vitamins [79] or a mixture of them [86][91]. In some pistachio studies [78][79][92], MS-vitamin has been replaced by Gamborg B5-vitamin mixture [99]. Some amino acids, such as L-valine and L-Cystein have been employed by some authors as alternative vitamins during pistachio micropropagation [81][91]. Interestingly, among standard MS-, DKW- or B5-vitamin mixtures, nicotinic-acid and pyridoxine-HCl were characterized as having a positive and significant effect, improving shoot length and the total healthy fresh weight of pistachio rootstock [98].
Recently, the application of RITA®® as an alternative approach to semi-solid media has enabled researchers to develop efficient protocols for mass-propagation of economically important plant species. In recent years, micropropagation of pistachio buds was improved using RITA®® through immersion of nodal and apical explants for 24 min every 16 h in MS medium containing 4 mg L−1 BA and 0.1 mg L−1 GA3 while reducing hyperhydricity and STN symptoms [79]. Therefore, RITA®® could be considered for the mass propagation of pistachio and its rootstocks.

References

  1. Whitehouse, W.E. The Pistachio Nut-a New Crop for the Western United States. Econ. Bot. 1957, 11, 281–321.
  2. Tilkat, E.; Işıkalan, Ç.; Onay, A. In vitro Propagation of Khinjuk Pistachio (Pistacia khinjuk Stocks) through Seedling Apical Shoot Tip Culture. Propag. Ornam. Plants 2005, 5, 124–128.
  3. Parfitt, D.; Kafkas, S.; Batlle, I.; Vargas, F.; Kallsen, C. Pistachio. In Fruit Breeding. Handbook of Plant Breeding; Badenes, M., Byrne, D., Eds.; Springer: Boston, MA, USA, 2012; Volume 8, pp. 803–826. ISBN 9781441907622.
  4. Khanazarov, A.A.; Chernova, G.M.; Rakhmonov, A.M.; Nikolyi, L.V.; Ablaeva, E.; Zaurov, D.E.; Molnar, T.J.; Eisenman, S.W.; Funk, C.R. Genetic Resources of Pistacia vera L. in Central Asia. Genet. Resour. Crop Evol. 2009, 56, 429–443.
  5. Ak, B.E.; Acar, I.; Sakar, E.; Gursoz, S. The Importance of Pistacia Species for Pistachio Production in Turkey. Acta Hortic. 2016, 1139, 183–188.
  6. Mir-Makhamad, B.; Bjørn, R.; Stark, S.; Spengler, R.N. Pistachio (Pistacia vera) Domestication and Dispersal out of Central Asia. Agronomy 2022, 12, 1758.
  7. Mohammed, A.A. Budding of Current Season Seedlings of Pistacia vera L. during Different Times in Late Summer. Turkish J. Agric.-Food Sci. Technol. 2022, 10, 191–193.
  8. Ouni, S.; Noguera-Artiaga, L.; Carbonell-Barrachina, A.; Ouerghui, I.; Jendoubi, F.; Rhouma, A.; Chelli-Chaabouni, A. Cultivar and Rootstock Effects on Growth, Yield and Nut Quality of Pistachio under Semi-Arid Conditions of South Mediterranean. Horticulturae 2022, 8, 606.
  9. Sheikhi, A.; Arab, M.M.; Brown, P.J.; Ferguson, L.; Akbari, M. Pistachio (Pistacia Spp.) Breeding. In Advances in Plant Breeding Strategies: Nut and Beverage Crops; Springer International Publishing: Cham, Switzerland, 2019; pp. 353–400.
  10. Pijut, P.M.; Beasley, R.R.; Lawson, S.S.; Palla, K.J.; Stevens, M.E.; Wang, Y. In vitro Propagation of Tropical Hardwood Tree Species—A Review (2001–2011). Propag. Ornam. Plants 2012, 12, 25–51.
  11. Ray, S.S.; Ali, M.N. Factors Affecting Macropropagation of Bamboo with Special Reference to Culm Cuttings: A Review Update. N. Z. J. For. Sci. 2017, 47, 17.
  12. Dunn, D.E.; Cole, J.C.; Smith, M.W. Position of Cut, Bud Retention and Auxins Influence Rooting of Pistacia chinensis. Sci. Hortic. 1996, 67, 105–110.
  13. Al Barazi, Z.; Schwabe, W.W. Rooting Softwood Cuttings of Adult Pistacia vera. J. Hortic. Sci. 1982, 57, 247–252.
  14. Mascarello, C.; Fascella, G.; Zizzo, G.V.; Mantovani, E.; Ruffoni, B. In vivo and In vitro Propagation of Pistacia lentiscus L. Acta Hortic. 2007, 764, 299–306.
  15. Joley, L.E. Experiences with Propagation of the Genus Pistacia. Proc. Int. Plant Propag. Soc. 1960, 10, 287–292.
  16. Joley, L.E.; Opitz, K.W. Further Experiments with Propagation of Pistacia. Proc. Int. Plant Propag. Soc. 1971, 21, 67–76.
  17. Assaf, R. Nouvelle Méthode de Multiplication Végétative de Rameaux Physiologiquement Adultes, de Pistachier, Noyer et Pacanier. Fruits 1977, 32, 309–319.
  18. Pair, J.C.; Khatamian, H. Propagation and Growing of the Chinese Pistache. Proc. Int. Plant Propag. Soc. 1982, 32, 502.
  19. Abousalim, A. Micropropagation and Micrografting of Pistachio (Pistacia vera and P. atlantica). Ph.D. Thesis, University of London, London, UK, 1990.
  20. Almehdi, A.A.; Parfitt, D.E.; Chan, H. Propagation of Pistachio Rootstock by Rooted Stem Cuttings. Sci. Hortic. 2002, 96, 359–363.
  21. Zohary, D.; Spiegel-Roy, P. Beginnings of Fruit Growing in the Old World. Science 1975, 187, 319–327.
  22. Harris, C.; Hong, X.; Gan, Q. Advanced Information Processing: A Neurofuzzy Approach; Jain, L.C., Ed.; Springer: Berlin/Heidelberg, Germany; Hamburg, Germany, 2002; ISBN 9783642083624.
  23. Mandalari, G.; Barreca, D.; Gervasi, T.; Roussell, M.A.; Klein, B.; Feeney, M.J.; Carughi, A. Pistachio Nuts (Pistacia vera L.): Production, Nutrients, Bioactives and Novel Health Effects. Plants 2022, 11, 18.
  24. Mudge, K.; Janick, J.; Scofield, S.; Goldschmidt, E.E. A History of Grafting. In Horticultural Reviews; John Wiley & Sons, Inc.: Hoboken, NJ, USA, 2009; pp. 437–493.
  25. Pina, A.; Errea, P. A Review of New Advances in Mechanism of Graft Compatibility–Incompatibility. Sci. Hortic. 2005, 106, 1–11.
  26. Melnyk, C.W.; Meyerowitz, E.M. Plant Grafting. Curr. Biol. 2015, 25, R183–R188.
  27. Ferguson, L.; Poss, J.A.; Grattan, S.R.; Grieve, C.M.; Wang, D.; Wilson, C.; Donovan, T.J.; Chao, C.-T. Pistachio Rootstocks Influence Scion Growth and Ion Relations under Salinity and Boron Stress. J. Am. Soc. Hortic. Sci. 2002, 127, 194–199.
  28. Jamshidi Goharrizi, K.; Baghizadeh, A.; Kalantar, M.; Fatehi, F. Combined Effects of Salinity and Drought on Physiological and Biochemical Characteristics of Pistachio Rootstocks. Sci. Hortic. 2020, 261, 108970.
  29. Arpaci, S.; Aksu, O.; Tekin, H. Determination of the Best Suitable Grafting Method on Different Pistachio Rootstocks. Acta Hortic. 1998, 470, 443–446.
  30. Onay, A. Micropropagation of Pistachio. In Micropropagation of Woody Trees and Fruits; Jain, S.M., Ishii, K., Eds.; Kluwer Academic Publishers: Amsterdam, The Netherlands, 2003; pp. 565–588. ISBN 1402011350.
  31. Ak, B.E.; Kandemir, M.; Sakar, E. Effect of Different Pistachio Rootstocks on Budding Methods Success and Growth of Scions. Acta Hortic. 2013, 981, 413–418.
  32. Barghchi, M.; Alderson, P.G. Pistachio (Pistacia vera L.). In Biotechnology in Agriculture and Forestry 5, Trees II; Bajaj, Y.P.S., Ed.; Springer: Berlin/Heidelberg, Germany, 1989; pp. 68–98. ISBN 9783642648625.
  33. Bajaj, Y.P.S. Cryopreservation of Plant Cell, Tissue, and Organ Culture for the Conservation of Germplasm and Biodiversity. In Cryopreservation of Plant Germplasm I. Biotechnology in Agriculture and Forestry; Springer: Berlin/Heidelberg, Germany, 1995; pp. 3–28.
  34. Engelmann, F. Plant Cryopreservation: Progress and Prospects. Vitr. Cell. Dev. Biol.-Plant 2004, 40, 427–433.
  35. Benelli, C. Plant Cryopreservation: A Look at the Present and the Future. Plants 2021, 10, 2744.
  36. Sharrock, S. Plant Conservation Report 2020: A Review of Progress in Implementation of the Global Strategy for Plant Conservation 2011–2020; Secretariat of the Convention on Biological Diversity; Montréal, Canada and Botanic Gardens Conservation International: Richmon, BC, Canada, 2020.
  37. Akdemir, H.; Onay, A. Biotechnological Approaches for Conservation of the Genus Pistacia. In Biodiversity and Conservation of Woody Plants. Sustainable Development and Biodiversity; Springer: Cham, Switzerland, 2017; pp. 221–244.
  38. Ozden-Tokatli, Y.; Ozudogru, E.A.; Gumusel, F.; Lambardi, M. Cryopreservation of Pistacia spp. Seeds by Dehydration and One-Step Freezing. CryoLetters 2007, 28, 83–94.
  39. Benmahioul, B.; Daguin, F.; Kaïd-Harche, M. Cryopreservation of Pistacia vera Embryonic Axes. J. For. Sci. 2016, 61, 182–187.
  40. Akdemir, H.; Süzerer, V.; Tilkat, E.; Yildirim, H.; Onay, A.; Çiftçi, Y.O. In vitro Conservation and Cryopreservation of Mature Pistachio (Pistacia vera L.) Germplasm. J. Plant Biochem. Biotechnol. 2013, 22, 43–51.
  41. Ozden Tokatli, Y.; Ozudogru, E.A.; Akdemir, H.; Gumusel, F.; De Carlo, A. Application of Cryopreservation for Pistachio Germplasm Conservation. Acta Hortic. 2009, 839, 245–251.
  42. Ozden-tokatli, Y.; Akdemir, H.; Tilkat, E.; Onay, A. Current Status and Conservation of Pistacia Germplasm. Biotechnol. Adv. 2010, 28, 130–141.
  43. Arora, K.; Rai, M.K.; Sharma, A.K. Tissue Culture Mediated Biotechnological Interventions in Medicinal Trees: Recent Progress. Plant Cell Tissue Organ Cult. 2022, 150, 267–287.
  44. Agrawal, A.; Singh, S.; Malhotra, E.V.; Meena, D.P.S.; Tyagi, R.K. In vitro Conservation and Cryopreservation of Clonally Propagated Horticultural Species. In Conservation and Utilization of Horticultural Genetic Resources; Springer Singapore: Singapore, 2019; pp. 529–578.
  45. El-Hawaz, R.F.; Adelberg, J.; Naylor-Adelberg, J.; Eisenreich, R.; Van der Meij, J. The Effect of Slow-Growth Strategy on a Production of Petunia × Hybrida Vilm. Microcuttings. Vitr. Cell. Dev. Biol. Plant 2019, 55, 433–441.
  46. Hammond Hammond, S.D.; Viehmannova, I.; Zamecnik, J.; Panis, B.; Hlasna Cepkova, P. Efficient Slow-Growth Conservation and Assessment of Clonal Fidelity of Ullucus Tuberosus Caldas Microshoots. Plant Cell Tissue Organ Cult. 2019, 138, 559–570.
  47. Priyanka, V.; Kumar, R.; Dhaliwal, I.; Kaushik, P. Germplasm Conservation: Instrumental in Agricultural Biodiversity—A Review. Sustainability 2021, 13, 6743.
  48. Kamińska, M.; Kęsy, J.; Trejgell, A. Abscisic Acid in Preservation of Taraxacum Pieninicum in the Form of Synthetic Seeds in Slow Growth Conditions. Plant Cell Tissue Organ Cult. 2021, 144, 295–312.
  49. Barghchi, M. In Vitro Storage of Plant Genetic Resources; Plant Physiology Division Biennial Report; Department of Scientific and Industrial Research: Palmerston North, New Zealand, 1986.
  50. Koç, İ.; Akdemir, H.; Onay, A.; Çiftçi, Y.Ö. Cold-Induced Genetic Instability in Micropropagated Pistacia lentiscus L. Plantlets. Acta Physiol. Plant. 2014, 36, 2373–2384.
  51. Chauhan, R.; Singh, V.; Quraishi, A. In vitro Conservation through Slow-Growth Storage. In Synthetic Seeds; Springer International Publishing: Cham, Switzerland, 2019; pp. 397–416.
  52. Lambardi, M.; Benelli, C.; Ozudogru, E.A.; Ozden-Tokatli, Y. Synthetic Seed Technology in Ornamental Plants. In Floriculture, Ornamental and Plant Biotechnology; Teixeira da Silva, J.A., Ed.; Global Science Books: Isleworth, UK, 2006; pp. 347–354.
  53. Benelli, C. Encapsulation of Shoot Tips and Nodal Segments for In vitro Storage of “Kober 5BB” Grapevine Rootstock. Horticulturae 2016, 2, 10.
  54. Faisal, M.; Alatar, A. Synthetic Seeds: Germplasm Regeneration, Preservation and Prospects; Springer International Publishing: Cham, Switzerland, 2019; ISBN 978-3-030-24630-3.
  55. Ozudogru, E.A.; Kirdok, E.; Kaya, E.; Capuana, M.; De Carlo, A.; Engelmann, F. Medium-Term Conservation of Redwood (Sequoia Sempervirens (D. Don.) Endl.) In vitro Shoot Cultures and Encapsulated Buds. Sci. Hortic. 2011, 127, 431–435.
  56. Ravi, D.; Arand, P. Production and Applications of Artificial Seeds: A Review. Int. Res. J. Biol. Sci. 2012, 1, 74–78.
  57. Islam, M.; Bari, M. In vitro Regeneration Protocol for Artificial Seed Production in an Important Medicinal Plant Mentha arvensis L. J. Bio-Sci. 2014, 20, 99–108.
  58. Ara, H.; Jaiswal, U.; Jaiswal, V.S. Synthetic Seed: Prospects and Limitations. Curr. Sci. 2000, 78, 1438–1444.
  59. Onay, A.; Jeffree, C.E.; Yeoman, M.M. Plant Regeneration from Encapsulated Embryoids and an Embryogenic Mass of Pistachio, Pistacia vera L. Plant Cell Rep. 1996, 15, 723–726.
  60. Piotto, B.; Di Noi, A. Seed Propagation of Mediterranean Trees and Shrubs. In Propagation of Mediterranean Trees and Shrubs from Seed–APAT; ANPA Handbook: Rome, Italy, 2003; pp. 61–82.
  61. Onay, A. In vitro Organogenesis and Embryogenesis of Pistachio, Pistacia vera L. Ph.D. Thesis, University of Edinburgh, Edinburgh, UK, 1996.
  62. Ghadirzadeh-Khorzoghi, E.; Jahanbakhshian-Davaran, Z.; Seyedi, S.M. Direct Somatic Embryogenesis of Drought Resistance Pistachio (Pistacia vera L.) and Expression Analysis of Somatic Embryogenesis-Related Genes. S. Afr. J. Bot. 2019, 121, 558–567.
  63. Abousalim, A.; Mantell, S.H. Micrografting of Pistachio (Pistacia vera L. Cv. Mateur). Plant Cell Tissue Organ Cult. 1992, 29, 231–234.
  64. Onay, A.; Pirinc, V.; Yildirim, H.; Basaran, D. In vitro Micrografting of Mature Pistachio (Pistacia vera Var. Siirt). Plant Cell Tissue Organ Cult. 2004, 77, 215–219.
  65. Abousalim, A.; Mantell, S.H. Micrografting of Pistachio (Pistacia vera L. cv. Siirt). In Protocols for Micropropagation of Woody Trees and Fruits; Springer: Dordrecht, The Netherlands, 2007; pp. 289–298.
  66. García-Pérez, P.; Lozano-Milo, E.; Landin, M.; Gallego, P.P. Machine Learning Unmasked Nutritional Imbalances on the Medicinal Plant Bryophyllum sp. Cultured In vitro. Front. Plant Sci. 2020, 11, 576177.
  67. George, E.F.; Hall, M.A.; De Klerk, G.-J. Plant Growth Regulators II: Cytokinins, Their Analogues and Antagonists. In Plant Propagation by Tissue Culture; Springer: Berlin/Heidelberg, Germany, 2008; pp. 205–226. ISBN 1402050046.
  68. Hameg, R.; Arteta, T.A.; Landin, M.; Gallego, P.P.; Barreal, M.E. Modeling and Optimizing Culture Medium Mineral Composition for In vitro Propagation of Actinidia Arguta. Front. Plant Sci. 2020, 11, 554905.
  69. García-Pérez, P.; Lozano-Milo, E.; Landín, M.; Gallego, P.P. Machine Learning Technology Reveals the Concealed Interactions of Phytohormones on Medicinal Plant In vitro Organogenesis. Biomolecules 2020, 10, 746.
  70. Arteta, T.A.; Hameg, R.; Landin, M.; Gallego, P.P.; Barreal, M.E. Artificial Neural Networks Elucidated the Essential Role of Mineral Nutrients versus Vitamins and Plant Growth Regulators in Achieving Healthy Micropropagated Plants. Plants 2022, 11, 1284.
  71. Lozano-Milo, E.; Landin, M.; Gallego, P.P.; García-Pérez, P. Machine Learning Deciphers Genotype and Ammonium as Key Factors for the Micropropagation of Bryophyllum sp. Medicinal Plants. Horticulturae 2022, 8, 987.
  72. Murashige, T.; Skoog, F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol. Plant. 1962, 15, 473–497.
  73. Benmahioul, B.; Kaïd-Harche, M.; Daguin, F. In vitro Regeneration of Pistacia vera L. from Nodal Explants. J. For. Sci. 2016, 62, 198–203.
  74. Bustamante-Garcia, M.A. Micropropagation and Rejuvenation of Pistacia Species and the Mechanism by Which Light Influences Root Initiation. Ph.D. Thesis, University of California, Davis, CA, USA, 1984.
  75. Yücel, S.; Onay, A.; Çolak, G.; Basaran, D. The Research of Obtaining from Pistacia vera L. Apical Bud and Nodal Bud by Micropropagation. Acta Hortic. 1991, 289, 167–170.
  76. Chan, H.M.; Almehdi, A.A.; Parfitt, D.E. Isolation of Protoplasts from Pistachio (Pistacia vera L.) for the Production of Somatic Embryos. In Proceedings of the XXVIth International Horticultural Congress Program and Abstracts, Toronto, ON, Canada, 11–17 August 2002; p. 84.
  77. Benmahioul, B.; Kaid-Harche, M.; Dorion, N.; Daguin, F. In vitro Embryo Germination and Proliferation of Pistachio (Pistacia vera L.). Sci. Hortic. 2009, 122, 479–483.
  78. Benmahioul, B.; Dorion, N.; Kaid-Harche, M.; Daguin, F. Micropropagation and Ex vitro Rooting of Pistachio (Pistacia vera L.). Plant Cell Tissue Organ Cult. 2012, 108, 353–358.
  79. Akdemir, H.; Süzerer, V.; Onay, A.; Tilkat, E.; Ersali, Y.; Çiftçi, Y.O. Micropropagation of the Pistachio and Its Rootstocks by Temporary Immersion System. Plant Cell Tissue Organ Cult. 2014, 117, 65–76.
  80. Barghchi, M.; Alderson, P.G. In vitro Propagation of Pistacia vera L. and the Commercial Cultivars Ohadi and Kalleghochi. J. Hortic. Sci. 1985, 60, 423–430.
  81. Kılınç, F.M.; Süzerer, V.; Çiftçi, Y.Ö.; Onay, A.; Yıldırım, H.; Uncuoğlu, A.A.; Tilkat, E.; Koç, İ.; Akdemir, Ö.F.; Metin, Ö.K. Clonal Micropropagation of Pistacia lentiscus L. and Assessment of Genetic Stability Using IRAP Markers. Plant Growth Regul. 2015, 75, 75–88.
  82. Driver, J.A.; Kuniyuki, A.H. In vitro Propagation of Paradox Walnut Rootstock. HortScience 1984, 19, 507–509.
  83. Lloyd, G.; McCown, B. Commericially-Feasible Micropropagation of Montain Lauerl, Kalmia Latifolia, by Use of Shoot-Tip Culture. Proc. Int. Plant Propagators Soc. 1980, 30, 421–427.
  84. González, A.; Frutos, D. In vitro Culture of Pistacia vera L. Embryos and Aged Trees Explants. In Plant Aging; Rodriguez, R., Sanchez Tames, R., Durzan, D.J., Eds.; Springer: Boston, MA, USA, 1990; pp. 335–338. ISBN 9781468457629.
  85. Abousalim, A.; Mantell, S.H. A Practical Method for Alleviating Shoot-Tip Necrosis Symtoms in In vitro Shoot Cultures of Pistacia vera cv. Mateur. J. Hortic. Sci. 1994, 69, 357–365.
  86. Dolcet-Sanjuan, R.; Claveria, E. Improved Shoot-Tip Micropropagation of Pistacia vera L. and the Beneficial Effects of Methyl Jasmonate. J. Am. Soc. Hortic. Sci. 1995, 120, 938–942.
  87. Marín, J.A.; García, E.; Lorente, P.; Andreu, P.; Arbeloa, A. A Novel Approach for Propagation of Recalcitrant Pistachio Cultivars That Sidesteps Rooting by Ex vitro Grafting of Tissue Cultured Shoot Tips. Plant Cell Tissue Organ Cult. 2016, 124, 191–200.
  88. Barghchi, M.; Alderson, P.G. The Control of Shoot Tip Necrosis in Pistacia vera L. In Vitro. Plant Growth Regul. 1996, 20, 31–35.
  89. Barghchi, M.; Alderson, P.G. In vitro Propagation of Pistacia Species. Acta Hortic. 1983, 131, 49–60.
  90. Yang, Z.; Lüdders, P. In vitro Propagation of Pistachio (Pistacia vera L.). Gartenbauwissenschaft 1993, 59, 30–34.
  91. Mederos-Molina, S.; Trujillo, M.I. Elimination of Browning Exudate and In vitro Development of Shoots in Pistacia vera L. cv. Mateur and Pistacia atlantica Desf. Culture. Acta Soc. Bot. Pol. 1999, 68, 21–24.
  92. Onay, A. Micropropagation of Pistachio from Mature Trees. Plant Cell Tissue Organ Cult. 2000, 60, 159–163.
  93. Akdemir, H.; Yıldırım, H.; Tilkat, E.; Onay, A.; Özden Çiftçi, Y. Prevention of Shoot Tip Necrosis Responses in In vitro-Proliferated Mature Pistachio Plantlets. In Proceedings of the Vitro Biology Meeting of the SIVB, Seattle, WA, USA, 27 July 2012; p. P-2003.
  94. Parfitt, D.E.; Almehdi, A.A. Use of High CO2 Atmosphere and Medium Modifications for the Successful Micropropagation of Pistachio. Sci. Hortic. 1994, 56, 321–329.
  95. Nezami-Alanagh, E.; Garoosi, G.-A.; Landín, M.; Gallego, P.P. Combining DOE with Neurofuzzy Logic for Healthy Mineral Nutrition of Pistachio Rootstocks In vitro Culture. Front. Plant Sci. 2018, 9, 1474.
  96. Nezami-Alanagh, E.; Garoosi, G.-A.; Landín, M.; Gallego, P.P. Computer-Based Tools Provide New Insight into the Key Factors That Cause Physiological Disorders of Pistachio Rootstocks Cultured In vitro. Sci. Rep. 2019, 9, 9740.
  97. Ozden-Tokatli, Y.; Ozudogru, E.A.; Akcin, A. In vitro Response of Pistachio Nodal Explants to Silver Nitrate. Sci. Hortic. 2005, 106, 415–426.
  98. Nezami-Alanagh, E.; Garoosi, G.-A.; Maleki, S.; Landín, M.; Gallego, P.P. Predicting Optimal In vitro Culture Medium for Pistacia vera Micropropagation Using Neural Networks Models. Plant Cell Tissue Organ Cult. 2017, 129, 19–33.
  99. Gamborg, O.L.; Miller, R.A.; Ojima, K. Nutrient Requirements of Suspension Cultures of Soybean Root Cells. Exp. Cell Res. 1968, 50, 151–158.
More
Information
Contributors MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to https://encyclopedia.pub/register : ,
View Times: 744
Revisions: 2 times (View History)
Update Date: 09 Mar 2023
1000/1000
Video Production Service