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HandWiki. Nanopore Sequencing. Encyclopedia. Available online: https://encyclopedia.pub/entry/34882 (accessed on 24 September 2026).
HandWiki. Nanopore Sequencing. Encyclopedia. Available at: https://encyclopedia.pub/entry/34882. Accessed September 24, 2026.
HandWiki. "Nanopore Sequencing" Encyclopedia, https://encyclopedia.pub/entry/34882 (accessed September 24, 2026).
HandWiki. (2022, November 16). Nanopore Sequencing. In Encyclopedia. https://encyclopedia.pub/entry/34882
HandWiki. "Nanopore Sequencing." Encyclopedia. Web. 16 November, 2022.
Nanopore Sequencing
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The content is sourced from: https://handwiki.org/wiki/Biology:Nanopore_sequencing

Nanopore sequencing is a third generation approach used in the sequencing of biopolymers — specifically, polynucleotides in the form of DNA or RNA. Using nanopore sequencing, a single molecule of DNA or RNA can be sequenced without the need for PCR amplification or chemical labeling of the sample. Nanopore sequencing has the potential to offer relatively low-cost genotyping, high mobility for testing, and rapid processing of samples with the ability to display results in real-time. Publications on the method outline its use in rapid identification of viral pathogens, monitoring ebola, environmental monitoring, food safety monitoring, human genome sequencing, plant genome sequencing, monitoring of antibiotic resistance, haplotyping and other applications.

polynucleotides biopolymers chemical labeling

References

  1. Lara Marks, Nanopore Sequencing https://www.whatisbiotechnology.org/index.php/science/summary/nanopore/nanopore-sequencing-makes-it-possible-to-decode-the
  2. "Single-molecule protein sensing in a nanopore: a tutorial". Chemical Society Reviews 47 (23): 8512–8524. November 2018. doi:10.1039/C8CS00106E. PMID 30328860. PMC 6309966. http://xlink.rsc.org/?DOI=C8CS00106E. 
  3. "Single-molecule protein unfolding in solid state nanopores". Journal of the American Chemical Society 131 (26): 9287–97. July 2009. doi:10.1021/ja901088b. PMID 19530678.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2717167
  4. "Probing Single DNA Molecule Transport Using Fabricated Nanopores". Nano Letters 4 (11): 2293–2298. November 2004. doi:10.1021/nl048654j. PMID 25221441. Bibcode: 2004NanoL...4.2293C.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4160839
  5. "Nanopore Sensing of Protein Folding". ACS Nano 11 (7): 7091–7100. July 2017. doi:10.1021/acsnano.7b02718. PMID 28693322.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=5564329
  6. "Solid-State Nanopore-Based DNA Sequencing Technology" (in en). Journal of Nanomaterials 2016: 1–13. 2016-05-30. doi:10.1155/2016/5284786.  https://dx.doi.org/10.1155%2F2016%2F5284786
  7. "Characterization of individual polynucleotide molecules using a membrane channel". Proceedings of the National Academy of Sciences of the United States of America 93 (24): 13770–3. November 1996. doi:10.1073/pnas.93.24.13770. PMID 8943010. Bibcode: 1996PNAS...9313770K.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=19421
  8. "Single-nucleotide discrimination in immobilized DNA oligonucleotides with a biological nanopore". Proceedings of the National Academy of Sciences of the United States of America 106 (19): 7702–7. May 2009. doi:10.1073/pnas.0901054106. PMID 19380741. Bibcode: 2009PNAS..106.7702S.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2683137
  9. "Nucleotide identification and orientation discrimination of DNA homopolymers immobilized in a protein nanopore". Nano Letters 8 (9): 3029–34. September 2008. doi:10.1021/nl802312f. PMID 18698831. Bibcode: 2008NanoL...8.3029P.  https://dx.doi.org/10.1021%2Fnl802312f
  10. "Continuous base identification for single-molecule nanopore DNA sequencing". Nature Nanotechnology 4 (4): 265–70. April 2009. doi:10.1038/nnano.2009.12. PMID 19350039. Bibcode: 2009NatNa...4..265C.  https://dx.doi.org/10.1038%2Fnnano.2009.12
  11. "The effects of diffusion on an exonuclease/nanopore-based DNA sequencing engine". The Journal of Chemical Physics 137 (21): 214903. December 2012. doi:10.1063/1.4766363. PMID 23231259. Bibcode: 2012JChPh.137u4903R.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4108639
  12. "Multiple base-recognition sites in a biological nanopore: two heads are better than one". Angewandte Chemie 49 (3): 556–9. 2010. doi:10.1002/anie.200905483. PMID 20014084.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3128935
  13. "Nucleotide discrimination with DNA immobilized in the MspA nanopore". PLOS ONE 6 (10): e25723. 2011. doi:10.1371/journal.pone.0025723. PMID 21991340. Bibcode: 2011PLoSO...625723M.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3186796
  14. "The structure of a mycobacterial outer-membrane channel". Science 303 (5661): 1189–92. February 2004. doi:10.1126/science.1094114. PMID 14976314. Bibcode: 2004Sci...303.1189F.  https://dx.doi.org/10.1126%2Fscience.1094114
  15. "Single-molecule DNA detection with an engineered MspA protein nanopore". Proceedings of the National Academy of Sciences of the United States of America 105 (52): 20647–52. December 2008. doi:10.1073/pnas.0807514106. PMID 19098105. Bibcode: 2008PNAS..10520647B.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2634888
  16. "Advanced Sequencing Technology Awards 2011". http://www.genome.gov/27545107. 
  17. Brinkerhoff, H; Kang, ASW; Liu, J; Aksimentiev, A; Dekker, C (4 November 2021). "Multiple rereads of single proteins at single-amino acid resolution using nanopores.". Science 374 (6574): 1509–1513. doi:10.1126/science.abl4381. PMID 34735217. PMC 8811723. Bibcode: 2021Sci...374.1509B. http://resolver.tudelft.nl/uuid:8d31363e-1576-466b-87ae-b692a6c05ab6. 
  18. "Challenges in DNA motion control and sequence readout using nanopore devices". Nanotechnology 26 (7): 074004. February 2015. doi:10.1088/0957-4484/26/7/074004. PMID 25642629. Bibcode: 2015Nanot..26g4004C.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=4710574
  19. "Electronic signatures of all four DNA nucleosides in a tunneling gap". Nano Letters 10 (3): 1070–5. March 2010. doi:10.1021/nl1001185. PMID 20141183. Bibcode: 2010NanoL..10.1070C.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2836180
  20. "Embedding a carbon nanotube across the diameter of a solid state nanopore". Journal of Vacuum Science & Technology B, Nanotechnology and Microelectronics: Materials, Processing, Measurement, and Phenomena 29 (5): 5. 2011. doi:10.1116/1.3628602. Bibcode: 2011JVSTB..29e3001S.  https://dx.doi.org/10.1116%2F1.3628602
  21. "DNA tunneling detector embedded in a nanopore". Nano Letters 11 (1): 279–85. January 2011. doi:10.1021/nl103873a. PMID 21133389. Bibcode: 2011NanoL..11..279I.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3020087
  22. "Drndić Laboratory – University of Pennsylvania". http://www.physics.upenn.edu/drndicgroup/dna-protein-analysis.html. 
  23. "Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays". Nano Letters 10 (6): 2237–44. June 2010. doi:10.1021/nl1012147. PMID 20459065. Bibcode: 2010NanoL..10.2237M.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2883017
  24. "Synchronous optical and electrical detection of biomolecules traversing through solid-state nanopores". The Review of Scientific Instruments 81 (1): 014301–014301–7. January 2010. doi:10.1063/1.3277116. PMID 20113116. Bibcode: 2010RScI...81a4301S.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2821415
  25. Liu, Mingxin; Clarke, Laurence J.; Baker, Susan C.; Jordan, Gregory J.; Burridge, Christopher P. (2019-12-30). "A practical guide to DNA metabarcoding for entomological ecologists". Ecological Entomology (Royal Entomological Society (Wiley)) 45 (3): 373–385. doi:10.1111/een.12831. ISSN 0307-6946.  ORCIDs: (ML 0000-0003-0436-4058). (SCB 0000-0002-7593-0267). https://dx.doi.org/10.1111%2Feen.12831
  26. Hamelin, Richard C.; Roe, Amanda D. (2019-09-10). "Genomic biosurveillance of forest invasive alien enemies: A story written in code". Evolutionary Applications (Blackwell) 13 (1): 95–115. doi:10.1111/eva.12853. ISSN 1752-4571. PMID 31892946.  ORCIDs: RCH 0000-0003-4006-532X). (ADR 0000-0002-1953-6062). http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=6935587
  27. Kennedy, Susan R.; Prost, Stefan; Overcast, Isaac; Rominger, Andrew J.; Gillespie, Rosemary G.; Krehenwinkel, Henrik (2020-02-10). "High-throughput sequencing for community analysis: the promise of DNA barcoding to uncover diversity, relatedness, abundances and interactions in spider communities". Development Genes and Evolution (Springer) 230 (2): 185–201. doi:10.1007/s00427-020-00652-x. ISSN 0949-944X. PMID 32040713.  ORCIDs: (SRK 0000-0002-1616-3985). (SP 0000-0002-6229-3596). (IO 0000-0001-8614-6892). (AJR 0000-0003-3755-4480). (RGG 0000-0003-0086-7424). (HK 0000-0001-5069-8601). http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=7127999
  28. Lawler, Richard R. (2018-10-21). "Emerging and Enduring Issues in Primate Conservation Genetics". Annual Review of Anthropology (Annual Reviews) 47 (1): 395–415. doi:10.1146/annurev-anthro-102317-050006. ISSN 0084-6570.  https://dx.doi.org/10.1146%2Fannurev-anthro-102317-050006
  29. "Sequencing single molecules of DNA". Current Opinion in Chemical Biology 10 (6): 628–37. December 2006. doi:10.1016/j.cbpa.2006.10.040. PMID 17113816.  https://dx.doi.org/10.1016%2Fj.cbpa.2006.10.040
  30. "Successful test launch for nanopore sequencing" (in En). Nature Methods 12 (4): 303–4. April 2015. doi:10.1038/nmeth.3327. PMID 25825834.  https://dx.doi.org/10.1038%2Fnmeth.3327
  31. "Recognizing a single base in an individual DNA strand: a step toward DNA sequencing in nanopores". Angewandte Chemie 44 (9): 1401–4. February 2005. doi:10.1002/anie.200462114. PMID 15666419.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1828035
  32. "Highly accurate classification of Watson-Crick basepairs on termini of single DNA molecules". Biophysical Journal 84 (2 Pt 1): 967–76. February 2003. doi:10.1016/S0006-3495(03)74913-3. PMID 12547778. Bibcode: 2003BpJ....84..967W.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1302674
  33. "Multiple base-recognition sites in a biological nanopore: two heads are better than one". Angewandte Chemie 49 (3): 556–9. 2010. doi:10.1002/anie.200905483. PMID 20014084. PMC 3128935. http://www3.interscience.wiley.com/journal/123210758/abstract?CRETRY=1&SRETRY=0. 
  34. "Toward single molecule DNA sequencing: direct identification of ribonucleoside and deoxyribonucleoside 5'-monophosphates by using an engineered protein nanopore equipped with a molecular adapter". Journal of the American Chemical Society 128 (5): 1705–10. February 2006. doi:10.1021/ja057123+. PMID 16448145.  https://dx.doi.org/10.1021%2Fja057123%2B
  35. Rusk, Nicole (2009-04-01). "Cheap Third-Generation Sequencing". Nature Methods 6 (4): 244–245. doi:10.1038/nmeth0409-244a.  https://dx.doi.org/10.1038%2Fnmeth0409-244a
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