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HandWiki. Polony Sequencing. Encyclopedia. Available online: https://encyclopedia.pub/entry/34647 (accessed on 25 September 2026).
HandWiki. Polony Sequencing. Encyclopedia. Available at: https://encyclopedia.pub/entry/34647. Accessed September 25, 2026.
HandWiki. "Polony Sequencing" Encyclopedia, https://encyclopedia.pub/entry/34647 (accessed September 25, 2026).
HandWiki. (2022, November 15). Polony Sequencing. In Encyclopedia. https://encyclopedia.pub/entry/34647
HandWiki. "Polony Sequencing." Encyclopedia. Web. 15 November, 2022.
Polony Sequencing
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The content is sourced from: https://handwiki.org/wiki/Biology:Polony_sequencing

Polony sequencing is an inexpensive but highly accurate multiplex sequencing technique that can be used to “read” millions of immobilized DNA sequences in parallel. This technique was first developed by Dr. George Church's group at Harvard Medical School. Unlike other sequencing techniques, Polony sequencing technology is an open platform with freely downloadable, open source software and protocols. Also, the hardware of this technique can be easily set up with a commonly available epifluorescence microscopy and a computer-controlled flowcell/fluidics system. Polony sequencing is generally performed on paired-end tags library that each molecule of DNA template is of 135 bp in length with two 17–18 bp paired genomic tags separated and flanked by common sequences. The current read length of this technique is 26 bases per amplicon and 13 bases per tag, leaving a gap of 4–5 bases in each tag.

epifluorescence amplicon flowcell/fluidics

References

  1. "Open Source Sequencing" (in en). http://arep.med.harvard.edu/Polonator/. 
  2. "After Early-Access Phase, Updated Polonator Is Ready for Rollout with $170K Price Tag" (in en). GenomeWeb. 2009-05-05. https://www.genomeweb.com/sequencing/after-early-access-phase-updated-polonator-ready-rollout-170k-price-tag. 
  3. "Dover's Polonator Unaffected by Danaher's Restructuring, Official Says" (in en). GenomeWeb. 2009-09-08. https://www.genomeweb.com/sequencing/dovers-polonator-unaffected-danahers-restructuring-official-says. 
  4. "The Polonator". 2015-04-03. http://www.polonator.org. 
  5. Adessi, C.; Matton, G.; Ayala, G.; Turcatti, G.; Mermod, J. J.; Mayer, P.; Kawashima, E. (2000-10-15). "Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms". Nucleic Acids Research 28 (20): E87. doi:10.1093/nar/28.20.e87. ISSN 1362-4962. PMID 11024189.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=110803
  6. Shendure, Jay; Porreca, Gregory J.; Reppas, Nikos B.; Lin, Xiaoxia; McCutcheon, John P.; Rosenbaum, Abraham M.; Wang, Michael D.; Zhang, Kun et al. (2005-09-09). "Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome" (in en). Science 309 (5741): 1728–1732. doi:10.1126/science.1117389. ISSN 0036-8075. PMID 16081699.  https://dx.doi.org/10.1126%2Fscience.1117389
  7. Shendure, Jay; Porreca, Gregory J.; Reppas, Nikos B.; Lin, Xiaoxia; McCutcheon, John P.; Rosenbaum, Abraham M.; Wang, Michael D.; Zhang, Kun et al. (2005-09-09). "Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome" (in en). Science 309 (5741): 1728–1732. doi:10.1126/science.1117389. ISSN 0036-8075. PMID 16081699.  https://dx.doi.org/10.1126%2Fscience.1117389
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