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HandWiki. Coomassie Brilliant Blue. Encyclopedia. Available online: https://encyclopedia.pub/entry/33505 (accessed on 11 October 2026).
HandWiki. Coomassie Brilliant Blue. Encyclopedia. Available at: https://encyclopedia.pub/entry/33505. Accessed October 11, 2026.
HandWiki. "Coomassie Brilliant Blue" Encyclopedia, https://encyclopedia.pub/entry/33505 (accessed October 11, 2026).
HandWiki. (2022, November 08). Coomassie Brilliant Blue. In Encyclopedia. https://encyclopedia.pub/entry/33505
HandWiki. "Coomassie Brilliant Blue." Encyclopedia. Web. 08 November, 2022.
Coomassie Brilliant Blue
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Coomassie Brilliant Blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry. Coomassie Brilliant Blue G-250 differs from Coomassie Brilliant Blue R-250 by the addition of two methyl groups. The name "Coomassie" is a registered trademark of Imperial Chemical Industries.

triphenylmethane dyes textile industry coomassie

References

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  2. Fox, M. R. (1987). Dye-makers of Great Britain 1856-1976 : A History of Chemists, Companies, Products and Changes. Manchester: Imperial Chemical Industries. p. 259. 
  3. Colour Index. 4 (3rd ed.). Bradford: Society of Dyers and Colourists. 1971. pp. 4397–4398. http://www.colour-index.org/help/3121_Triarylmethane.pdf. Retrieved 2010-07-20. 
  4. "Procédé de production de colorants de la série du triarylméthane" FR patent 474260, issued 1915-02-16, assigned to Bayer http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=FR474260
  5. Weiler, Max, "Blue Triphenylmethane Dye", US patent 1218232, issued 1917-03-06 http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=US1218232
  6. "Manufacture of Triarylmethane-dyestuffs" GB patent 275609, issued 1927-11-03, assigned to IG Farbenindustrie http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=GB275609
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  8. Bradford, Marion M. (1976). "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding". Analytical Biochemistry 72 (1–2): 248–254. doi:10.1016/0003-2697(76)90527-3. PMID 942051. http://hoffman.cm.utexas.edu/courses/bradford_assay.pdf. 
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  10. Fazekas de St. Groth, S.; Webster, R. G.; Datyner, A. (1963). "Two new staining procedures for quantitative estimation of proteins on electrophoretic strips". Biochimica et Biophysica Acta 71: 377–391. doi:10.1016/0006-3002(63)91092-8. PMID 18421828.  https://dx.doi.org/10.1016%2F0006-3002%2863%2991092-8
  11. Meyer, T. S.; Lambert, B. L. (1965). "Use of Coomassie brilliant blue R250 for the electrophoresis of microgram quantities of parotid saliva proteins on acrylamide-gel strips". Biochimica et Biophysica Acta (BBA) - General Subjects 107 (1): 144–145. doi:10.1016/0304-4165(65)90403-4. PMID 4159310.  https://dx.doi.org/10.1016%2F0304-4165%2865%2990403-4
  12. Altschul, A. M.; Evans, W. J. (1967). Zone electrophoresis with polyacrylamide gel. Methods in Enzymology. 11. pp. 179–186. doi:10.1016/S0076-6879(67)11019-7. ISBN 9780121818609. . Page 184 personal communication from W. J. Saphonov. https://dx.doi.org/10.1016%2FS0076-6879%2867%2911019-7
  13. Diezel, W.; Kopperschläger, G.; Hofmann, E. (1972). "An improved procedure for protein staining in polyacrylamide gels with a new type of Coomassie Brilliant Blue". Analytical Biochemistry 48 (2): 617–620. doi:10.1016/0003-2697(72)90117-0. PMID 4115985.  https://dx.doi.org/10.1016%2F0003-2697%2872%2990117-0
  14. Neuhoff, V.; Stamm, R.; Eibl, H. (1985). "Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: a systematic analysis". Electrophoresis 6 (9): 427–448. doi:10.1002/elps.1150060905.  https://dx.doi.org/10.1002%2Felps.1150060905
  15. Candiano, G.; Bruschi, M.; Musante, L.; Santucci, L.; Ghiggeri, G. M.; Carnemolla, B.; Orecchia, P.; Zardi, L. et al. (2004). "Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis". Electrophoresis 25 (9): 1327–1333. doi:10.1002/elps.200305844. PMID 15174055.  https://dx.doi.org/10.1002%2Felps.200305844
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  17. Pink, M.; Verma, N.; Rettenmeier, A. W.; Schmitz-Spanke, S. (2010). "CBB staining protocol with higher sensitivity and mass spectrometric compatibility". Electrophoresis 31 (4): 593–598. doi:10.1002/elps.200900481. PMID 20162584.  https://dx.doi.org/10.1002%2Felps.200900481
  18. Bradford, M. M. (1976). "Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding". Analytical Biochemistry 72 (1–2): 248–254. doi:10.1016/0003-2697(76)90527-3. PMID 942051.  https://dx.doi.org/10.1016%2F0003-2697%2876%2990527-3
  19. Congdon, Robert W.; Muth, Gregory W.; Splittgerber, Allan G. (September 1993). "The binding interaction of Coomassie blue with proteins.". Analytical Biochemistry 213 (2): 407–413. doi:10.1006/abio.1993.1439. PMID 7694523.  https://dx.doi.org/10.1006%2Fabio.1993.1439
  20. Schägger, H.; Jagow, G. (1991). "Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form". Analytical Biochemistry 199 (2): 223–231. doi:10.1016/0003-2697(91)90094-A. PMID 1812789.  https://dx.doi.org/10.1016%2F0003-2697%2891%2990094-A
  21. Wittig, I.; Braun, H. P.; Schägger, H. (2006). "Blue native PAGE". Nature Protocols 1 (1): 418–428. doi:10.1038/nprot.2006.62. PMID 17406264.  https://dx.doi.org/10.1038%2Fnprot.2006.62
  22. Welinder, Charlotte; Ekblad, Lars (2011). "Coomassie Staining as Loading Control in Western Blot Analysis". Journal of Proteome Research 10 (3): 1416–1419. doi:10.1021/pr1011476. PMID 21186791.  https://dx.doi.org/10.1021%2Fpr1011476
  23. Peng, W.; Cotrina, M. L.; Han, X.; Yu, H.; Bekar, L.; Blum, L.; Takano, T.; Tian, G. F. et al. (2009). "Systemic administration of an antagonist of the ATP-sensitive receptor P2X7 improves recovery after spinal cord injury". Proceedings of the National Academy of Sciences of the United States of America 106 (30): 12489–12493. doi:10.1073/pnas.0902531106. PMID 19666625.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2718350
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  25. "Blue M&Ms 'mend spinal injuries'". Telegraph. 2009-07-28. https://www.telegraph.co.uk/news/science/science-news/5921266/Blue-MandMs-mend-spinal-injuries.html. 
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  28. "Drug Trials Snapshots: TissueBlue". 20 December 2019. http://www.fda.gov/drugs/drug-approvals-and-databases/drug-trials-snapshots-tissueblue. 
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