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HandWiki. Single Molecule Real Time Sequencing. Encyclopedia. Available online: https://encyclopedia.pub/entry/32216 (accessed on 25 September 2026).
HandWiki. Single Molecule Real Time Sequencing. Encyclopedia. Available at: https://encyclopedia.pub/entry/32216. Accessed September 25, 2026.
HandWiki. "Single Molecule Real Time Sequencing" Encyclopedia, https://encyclopedia.pub/entry/32216 (accessed September 25, 2026).
HandWiki. (2022, November 01). Single Molecule Real Time Sequencing. In Encyclopedia. https://encyclopedia.pub/entry/32216
HandWiki. "Single Molecule Real Time Sequencing." Encyclopedia. Web. 01 November, 2022.
Single Molecule Real Time Sequencing
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The content is sourced from: https://handwiki.org/wiki/Biology:Single_molecule_real_time_sequencing

Single molecule real time sequencing (SMRT) is a parallelized single molecule DNA sequencing method. Single molecule real time sequencing utilizes a zero-mode waveguide (ZMW). A single DNA polymerase enzyme is affixed at the bottom of a ZMW with a single molecule of DNA as a template. The ZMW is a structure that creates an illuminated observation volume that is small enough to observe only a single nucleotide of DNA being incorporated by DNA polymerase. Each of the four DNA bases is attached to one of four different fluorescent dyes. When a nucleotide is incorporated by the DNA polymerase, the fluorescent tag is cleaved off and diffuses out of the observation area of the ZMW where its fluorescence is no longer observable. A detector detects the fluorescent signal of the nucleotide incorporation, and the base call is made according to the corresponding fluorescence of the dye.

single nucleotide single molecule real time

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