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HandWiki. Massive Parallel Sequencing. Encyclopedia. Available online: https://encyclopedia.pub/entry/27973 (accessed on 25 September 2026).
HandWiki. Massive Parallel Sequencing. Encyclopedia. Available at: https://encyclopedia.pub/entry/27973. Accessed September 25, 2026.
HandWiki. "Massive Parallel Sequencing" Encyclopedia, https://encyclopedia.pub/entry/27973 (accessed September 25, 2026).
HandWiki. (2022, September 29). Massive Parallel Sequencing. In Encyclopedia. https://encyclopedia.pub/entry/27973
HandWiki. "Massive Parallel Sequencing." Encyclopedia. Web. 29 September, 2022.
Massive Parallel Sequencing
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The content is sourced from: https://handwiki.org/wiki/Biology:Massive_parallel_sequencing

Massive parallel sequencing or massively parallel sequencing is any of several high-throughput approaches to DNA sequencing using the concept of massively parallel processing; it is also called next-generation sequencing (NGS) or second-generation sequencing. Some of these technologies emerged in 1994-1998 and have been commercially available since 2005. These technologies use miniaturized and parallelized platforms for sequencing of 1 million to 43 billion short reads (50-400 bases each) per instrument run. Many NGS platforms differ in engineering configurations and sequencing chemistry. They share the technical paradigm of massive parallel sequencing via spatially separated, clonally amplified DNA templates or single DNA molecules in a flow cell. This design is very different from that of Sanger sequencing—also known as capillary sequencing or first-generation sequencing—that is based on electrophoretic separation of chain-termination products produced in individual sequencing reactions.

massively parallel clonally parallel sequencing

References

  1. Matthew W. Anderson; Iris Schrijver (2010). "Next Generation DNA Sequencing and the Future of Genomic Medicine". Genes 1 (1): 38–69. doi:10.3390/genes1010038. PMID 24710010.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3960862
  2. Tracy Tucker; Marco Marra; Jan M. Friedman (Aug 2009). "Massively Parallel Sequencing The Next Big Thing in Genetic Medicine". Am J Hum Genet 85 (2): 142–54. doi:10.1016/j.ajhg.2009.06.022. PMID 19679224.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2725244
  3. Andreas Von Bubnoff (2008). "Next-generation sequencing: the race is on". Cell 132 (5): 721–723. doi:10.1016/j.cell.2008.02.028. PMID 18329356.  https://dx.doi.org/10.1016%2Fj.cell.2008.02.028
  4. "2008 Release: NHGRI Seeks DNA Sequencing Technologies Fit for Routine Laboratory and Medical Use". Genome.gov. http://www.genome.gov/27527585. 
  5. "Archived copy". http://systems.illumina.com/systems/hiseq_2500_1500/performance_specifications.html. 
  6. "Archived copy". http://genomics.ed.ac.uk/blog/hiseq-v4-here-and-it-delivers. 
  7. Jingyue Ju, Zengmin Li, John Robert Edwards, Yasuhiro Itagaki, "Massive parallel method for decoding DNA and RNA", patent US7790869B2, published 2010-09-07 http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=US7790869B2
  8. "Accurate whole human genome sequencing using reversible terminator chemistry". Nature 456 (7218): 53–9. Nov 6, 2008. doi:10.1038/nature07517. PMID 18987734. Bibcode: 2008Natur.456...53B.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2581791
  9. "Sequence and structural variation in a human genome uncovered by short-read, massively parallel ligation sequencing using two-base encoding". Genome Res 19 (9): 1527–41. Sep 2009. doi:10.1101/gr.091868.109. PMID 19546169.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2752135
  10. "Ion Torrent". http://www.allseq.com/knowledgebank/sequencing-platforms/life-technologies-ion-torrent/. 
  11. "Human Genome Sequencing Using Unchained Base Reads on Self-assembling DNA Nanoarrays". Science 327 (5961): 78–81. 2010. doi:10.1126/science.1181498. PMID 19892942. Bibcode: 2010Sci...327...78D.  https://dx.doi.org/10.1126%2Fscience.1181498
  12. "Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome". Science 309 (5741): 1728–32. 2005. doi:10.1126/science.1117389. PMID 16081699. Bibcode: 2005Sci...309.1728S. https://semanticscholar.org/paper/8a21c5ea21a3b6b9ac217336e85796c4a9c8868b. 
  13. "Accurate whole genome sequencing and haplotyping from 10-20 human cells". Nature 487 (7406): 190–195. 2012. doi:10.1038/nature11236. PMID 22785314. Bibcode: 2012Natur.487..190P.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3397394
  14. Pacific Biosciences Introduces New Chemistry With Longer Read Lengths to Detect Novel Features in DNA Sequence and Advance Genome Studies of Large Organisms http://globenewswire.com/news-release/2013/10/03/577891/10051072/en/Pacific-Biosciences-Introduces-New-Chemistry-With-Longer-Read-Lengths-to-Detect-Novel-Features-in-DNA-Sequence-and-Advance-Genome-Studies-of-Large-Organisms.html
  15. Lex Nederbragt (2013-07-05). "De novo bacterial genome assembly: a solved problem?". http://flxlexblog.wordpress.com/2013/07/05/de-novo-bacterial-genome-assembly-a-solved-problem/. 
  16. Karl V. Voelkerding; Shale Dames; Jacob D. Durtschi (September 2010). "Diagnostic Next Generation Sequencing". J Mol Diagn 12 (5): 539–51. doi:10.2353/jmoldx.2010.100043. PMID 20805560.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2928417
  17. Chee-Seng, Ku; En Yun, Loy; Yudi, Pawitan; and Kee-Seng, Chia. Next Generation Sequencing Technologies and Their Applications. In: Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd: Chichester.April 2010
  18. Metzker ML (Jan 2010). "Sequencing technologies - the next generation.". Nat Rev Genet 11 (1): 31–46. doi:10.1038/nrg2626. PMID 19997069.  https://dx.doi.org/10.1038%2Fnrg2626
  19. "Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations". Proc Natl Acad Sci U S A 100 (15): 8817–22. Jul 22, 2003. doi:10.1073/pnas.1133470100. PMID 12857956. Bibcode: 2003PNAS..100.8817D.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=166396
  20. George M. Church, Rob Mitra, "Replica amplification of nucleic acid arrays", patent US6485944B1, published 2002-11-26 http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=US6485944B1
  21. "In situ localized amplification and contact replication of many individual DNA molecules". Nucleic Acids Res. 27 (24): e34; 1–6. Dec 1999. doi:10.1093/nar/27.24.e34. PMID 10572186.  http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=148757
  22. George M Church, Gregory J Porreca, Abraham Rosenbaum, Jay Shendure, "Nanogrid rolling circle dna sequencing", patent application WO2007120208A3, published 2008-08-28 http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=WO2007120208A3
  23. Radoje Drmanac, Matthew J. Callow, Snezana Drmanac, Brian K. Hauser, George Yeung, "Single molecule arrays for genetic and chemical analysis", patent US8445194B2, published 2013-05-21 http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=US8445194B2
  24. Laurent Farinelli, Eric Kawashima, Pascal Mayer (fr), "Method of nucleic acid amplification", patent application WO1998044151A1, published 1998-10-08 http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=WO1998044151A1
  25. Laurent Farinelli, Eric Kawashima, Pascal Mayer (fr), "Method of nucleic acid sequencing", patent application WO1998044152A1, published 1998-10-08 http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=WO1998044152A1
  26. P. Mayer et al., presented at the Fifth International Automation in Mapping and DNA Sequencing Conference, St. Louis, MO, USA (October 7–10, 1998). DNA colony massively parallel sequencing ams98 presentation "A very large scale, high throughput and low cost DNA sequencing method based on a new 2-dimensional DNA auto-patterning process". http://www.slideshare.net/pascalmayer/dna-colony-massively-parrallel-sequencing-ams98-presentation DNA colony massively parallel sequencing ams98 presentation. 
  27. US Patent 5,641,658 Method for performing amplification of nucleic acid with two primers bound to a single solid support. Inventors: Christopher P. Adams, Stephen Joseph Kron https://www.google.com/patents/US5641658
  28. M. Ronaghi; S. Karamohamed; B. Pettersson; M. Uhlen; P. Nyren (1996). "Real-time DNA sequencing using detection of pyrophosphate release". Analytical Biochemistry 242 (1): 84–9. doi:10.1006/abio.1996.0432. PMID 8923969.  https://dx.doi.org/10.1006%2Fabio.1996.0432
  29. High-throughput DNA sequencing –concepts and limitations, Martin Kircher and Janet Kelso, Bioessays 32: 524–536, 2010 WILEY Periodicals Inc.
  30. Shankar Balasubramanian, "Polynucleotide sequencing", patent application WO2001023610A2, published 2001-04-05 http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=WO2001023610A2
  31. "Assay Technology". Illumina. http://www.illumina.com/company/assay_technology.ilmn. 
  32. "True Single Molecule Sequencing (tSMS™): Helicos BioSciences". Helicosbio.com. http://www.helicosbio.com/Technology/tabid/64/Default.aspx. 
  33. "Fundamentals of 2 Base Encoding and Color Space". Appliedbiosystems.cnpg.com. http://appliedbiosystems.cnpg.com/Video/flatFiles/699/index.aspx. 
  34. "Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data". Nat Methods 10 (6): 563–9. Jun 2013. doi:10.1038/nmeth.2474. PMID 23644548.  https://dx.doi.org/10.1038%2Fnmeth.2474
  35. Monica Heger (March 5, 2013). "PacBio Users Report Progress in Long Reads for Plant Genome Assembly, Tricky Regions of Human Genome". http://www.genomeweb.com/sequencing/pacbio-users-report-progress-long-reads-plant-genome-assembly-tricky-regions-hum. 
  36. "PacBio Launches Higher-Throughput, Lower-Cost Single-Molecule Sequencing System". October 2015. https://www.genomeweb.com/business-news/pacbio-launches-higher-throughput-lower-cost-single-molecule-sequencing-system. 
  37. http://www.bio-itworld.com/2015/9/30/pacbio-announces-sequel-sequencing-system.aspx
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