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1 VitD plays a small, yet significant, role in the pathogenesis of AITD, probably dependent on other factors. After the onset of AITD its consequences may generate a vicious cycle contributing to aggravation of the deficiency. + 2249 word(s) 2249 2020-09-16 06:28:16 |
2 VitD plays a small, yet significant, role in the pathogenesis of AITD, dependent on the contribution of other factors. After the onset of AITD, its consequences may generate a vicious cycle contributing to aggravation of the deficiency. Meta information modification 2249 2020-09-16 20:20:31 | |
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Vieira, I.H.; Rodrigues, D.; Paiva, I.; Vieira, I. VitD and Autoimmune Thyroid Disease. Encyclopedia. Available online: (accessed on 28 November 2023).
Vieira IH, Rodrigues D, Paiva I, Vieira I. VitD and Autoimmune Thyroid Disease. Encyclopedia. Available at: Accessed November 28, 2023.
Vieira, Inês Henriques, Dírcea Rodrigues, Isabel Paiva, Inês Vieira. "VitD and Autoimmune Thyroid Disease" Encyclopedia, (accessed November 28, 2023).
Vieira, I.H., Rodrigues, D., Paiva, I., & Vieira, I.(2020, September 16). VitD and Autoimmune Thyroid Disease. In Encyclopedia.
Vieira, Inês Henriques, et al. "VitD and Autoimmune Thyroid Disease." Encyclopedia. Web. 16 September, 2020.
VitD and Autoimmune Thyroid Disease

Vitamin D is a steroid hormone traditionally connected to phosphocalcium metabolism. The discovery of pleiotropic expression of its receptor and of the enzymes involved in its metabolism have led to exploration of the other roles of this vitamin, namely on autoimmune thyroid disease. Most of the existing data support a relationship between vitamin D deficiency and a greater tendency for development and/or higher titers of antibodies linked to Hashimoto's thyroiditis, Graves' disease, and/or postpartum thyroiditis. The nature of this relationship is yet unknown, it may be due to vitamin D's immunoregulatory role, emerge as a consequence of the autoimmune disease, or be a result physiopathological process underlying the autoimmune disease.

Our thesis is that, due to its immunoregulatory role, vitamin D plays a minor role in conjunction with myriad other factors. In some cases, a vicious cycle is generated, thus contributing to the deficiency and aggravating the autoimmune process.

Vitamin D Autoimmune thyroid disease Vitamin D receptor Graves’ disease Hashimoto thyroiditis

1. Introduction

Vitamin D is a steroid hormone traditionally connected to phosphocalcium metabolism. The discovery of pleiotropic expression of its receptor and of the enzymes involved in its metabolism have led to exploration of the other roles of this vitamin. The influence of vitamin D on autoimmune disease—namely, on autoimmune thyroid disease—has been widely studied.

The term vitamin D (VitD) encompasses a group of steroid compounds, namely VitD2 (ergocalciferol) and VitD3 (cholecalciferol) [1].

Its main functions are the regulation of phosphocalcium metabolism and the promotion of bone homeostasis. However, the discovery of the widespread expression of the VitD receptor (VDR) and the enzymes responsible for its metabolism suggests the pleiotropic role of this vitamin and its influence in several diseases[2][3]. An immunomodulatory role is evident and its influence on the development of autoimmune diseases (AID) has been proposed. Autoimmune thyroid disease (AITD) is the most common organ-specific AID[3] and several studies have been carried out to explore the role of VitD in its development and course, as well as the possible impact of supplementation.

2. What is the Nature of the Relationship between Vitamin D levels and Autoimmune Thyroid Disease?

Although there exists some inconsistency in the results of the studies carried out so far, most of the data are consistent with the presence of an association between vitamin D and AITD. However, there are several possible interpretations for this association. 

The most commonly cited explanation is the decrease in the immunomodulatory role of 1,25(OH)2D, in patients with deficiency, contributing to the development of AID. However, the data obtained to date are mostly resultant from cross-sectional studies, which do not allow for the establishment of causal effects. It is, therefore, essential to evaluate alternative explanatory models.

Some authors have raised the possibility that the various data favoring the involvement of VitD in AITD reflect a consequence, rather than a cause, of the disease. AID may lead to VitD deficiency by causing incapacitation and lower sunlight exposure, malabsorption, and use of corticosteroids[4][5]. In hyperthyroidism, there may be accelerated bone turnover. Kozai et al. found marked decreases in 1,25(OH)2D and CYP27B1 expression in rats with T3-induced hyperthyroidism[6]. In HT, the increase in fat mass caused by hypothyroidism could contribute to the deficiency[7]. Botello et al. studied 88 patients with long-term HT and found a positive correlation between 25(OH)D levels, fT4, and (contrary to expectations) Th17 and TNFα. The authors hypothesized that low levels of fT4 are predictors of a deficiency of 25(OH)D and that the long evolution of the disease and treatment of hypothyroidism are related to a decrease in cytotoxic immune response, regardless of the levels of 25(OH)D[8]. The coexistence of AITD with other AID, such as celiac disease, also deserves consideration. Celiac disease leads to malabsorption with deficiency of several nutrients[9], including VitD[10]and it is associated with an increased risk of developing other AIDs[9][10]. The presence of biopsy-proven celiac disease in patients with AITD is small, around 1.6% according to a recent meta-analysis (although there may be some underdiagnosis)[11]; therefore, it cannot fully explain the reported lower values of VitD in all AITD patients. However, it is likely to contribute to this association in patients in which both diseases coexist. A group of HT patients with positive transglutaminase antibodies and no symptoms of celiac disease were divided, receiving gluten-free vs. gluten-containing diets. The former group, but not the second one, experienced a reduction in antibody titers and an increase in VitD levels [12]. However, the possibility of VitD deficiency being exclusively a consequence of AIDseems unlikely, given that this relationship has been found in several studies, independently of factors such as age, body mass index, thyroid function tests (i.e., presence of hyper-, hypo-, or euthyroidism) and presence or absence of other AIDs. Furthermore, in a study that evaluated patients with GD and 25(OH)D insufficiency, no statistically significant difference was found in the values ​​of 25(OH)D at baseline and 1 to 2 years after hyperthyroidism therapy (with achievement of euthyroidism)[13]. Therefore, contrary to what would be expected if low levels of VitD were a consequence of the autoimmune disease, treating the autoimmune disease does not improve VitD status.  

Another possibility is that the lower levels of 25(OH)D in AID are the result of a pathophysiological mechanism involved in the development of the disease; that is, VDR dysfunction caused by chronic infection by intra-phagocytic microorganisms[7]. This dysfunction could lead to lower production of the antimicrobial peptides that would usually result from activation of VDR. VDR dysfunction could also lead to lesser expression of 24-hydroxylase, with a consequent increase in 1,25(OH)2D levels. Excess 1,25(OH)2D has the ability to displace ligands of nuclear receptors such as α-thyroid, glucocorticoids, and androgens, which can lead to glandular dysfunction [14]. Elevated levels of 1,25(OH)2D further bind to the pregnane X receptor and inhibit the synthesis of 25(OH)D in the liver. In this context, the various data pointing towards a relationship between AID and VitD deficiency may be explained by the fact that the metabolite usually measured is 25(OH)D[15]. This is a counterintuitive hypothesis, with some theoretical background but with little data to support or contradict it directly, as 1,25(OH)2D is rarely quantified. However, some of the above-mentioned studies on VitD supplementation reported elevated PTH and unchanged or slightly lower calcium values,​​ associated with a deficiency of 25(OH)D at baseline with a tendency towards normalization after VitD supplementation[16][17][18]. This does not support the possibility that there is an increase in 1,25(OH)2D in AITD concealed by the quantification of 25(OH)D. Although it may be argued that PTH level elevation and lowering of calcium levels may be explained by VDR dysfunction, it is unlikely that such alterations were susceptible to correction by VitD supplementation, as it would not correct the primary mechanism. The fact that VitD supplementation has shown some beneficial effects on AI parameters is also against this hypothesis.

Analyzing the current evidence, we conclude that, despite that a direct and marked contribution of VitD levels alone in the pathogenesis of AITD is unlikely, given the marked inconsistency of the data, a minor contribution is probable, as the existence of an association has been supported by the majority of the studies cited above (refer to Section 5.2. Data on vitamin D levels and autoimmune thyroid disease). Therefore, it is plausible that the levels of VitD, the polymorphisms of its receptor[19][20][21][22], and the enzymes that govern its metabolism[23] influence its regulatory capacity and, thus, it likely plays a small, yet significant, role in the development and course of AITD. It is likely that this contribution depends upon a multiplicity of other factors, such as age and gender, sex hormones [24][25], and micronutrients[26]. Genetic, epigenetic, and other endogenous and environmental factors which contribute to the predisposition to AITD may also influence this correlation, explaining some of the inconsistency in the results obtained in different populations. The above-mentioned consequences of AITD (e.g., incapacitation, lower sunlight exposure, obesity in hypothyroidism, and increased bone turnover in hyperthyroidism) and, in some cases, the coexistence of other AID may generate a vicious cycle and contribute to the observed relationship.


3. What is the role of vitamin D supplementation in autoimmune thyroid disease?

Given the paucity of data in this regard, a logical approach is to aim for VitD levels within the reference ranges suggested by international guidelines. The Institute of Medicine considers 20 ng/mL to be sufficient for most of the general population[27]. The Endocrine Society Guidelines, focused on individuals with risk of VitD deficiency, identify an optimal level of 25(OH)D > 30 ng/mL and that values ​​up to 100 ng/mL (250 nmol/L) are safe (as they do not cause hypercalcemia)[28].

In the future, more data from investigations with a larger number of individuals, a more global scope, and involving year-round evaluations of VitD levels are necessary, in order to provide more uniform and consistent answers to this question.



  1. Kmiec P, Sworczak K. Vitamin D in thyroid disorders. Exp Clin Endocrinol Diabetes Off J Ger Soc Endocrinol Ger Diabetes Assoc. 2015 Jul;123(7):386–93.
  2. Muscogiuri G, Mitri J, Mathieu C, Badenhoop K, Tamer G, Orio F, et al. Mechanisms in endocrinology: vitamin D as a potential contributor in endocrine health and disease. Eur J Endocrinol. 2014 Sep;171(3):R101-110.
  3. Kim D. The Role of Vitamin D in Thyroid Diseases. Int J Mol Sci. 2017 Sep 12;18(9).
  4. Kivity S, Agmon-Levin N, Zisappl M, Shapira Y, Nagy EV, Danko K, et al. Vitamin D and autoimmune thyroid diseases. Cell Mol Immunol. 2011 May;8(3):243–7.
  5. Bizzaro G, Shoenfeld Y. Vitamin D and thyroid autoimmune diseases: the known and the obscure. Immunol Res. 2015 Feb;61(1–2):107–9.
  6. Kozai M, Yamamoto H, Ishiguro M, Harada N, Masuda M, Kagawa T, et al. Thyroid hormones decrease plasma 1α,25-dihydroxyvitamin D levels through transcriptional repression of the renal 25-hydroxyvitamin D3 1α-hydroxylase gene (CYP27B1). Endocrinology. 2013 Feb;154(2):609–22.
  7. Hu S, Rayman MP. Multiple Nutritional Factors and the Risk of Hashimoto’s Thyroiditis. Thyroid Off J Am Thyroid Assoc. 2017 May;27(5):597–610.
  8. Botelho IMB, Moura Neto A, Silva CA, Tambascia MA, Alegre SM, Zantut-Wittmann DE. Vitamin D in Hashimoto’s thyroiditis and its relationship with thyroid function and inflammatory status. Endocr J. 2018 Oct 29;65(10):1029–37.
  9. Kawicka A, Regulska-Ilow B, Regulska-Ilow B. Metabolic disorders and nutritional status in autoimmune thyroid diseases. Postepy Hig Med Doswiadczalnej Online. 2015 Jan 2;69:80–90.
  10. Walker MD, Zylberberg HM, Green PHR, Katz MS. Endocrine complications of celiac disease: a case report and review of theliterature. Endocr Res. 2019 May;44(1–2):27–45.
  11. Roy A, Laszkowska M, Sundström J, Lebwohl B, Green PHR, Kämpe O, et al. Prevalence of Celiac Disease in Patients with Autoimmune Thyroid Disease: AMeta-Analysis. Thyroid Off J Am Thyroid Assoc. 2016 Jul;26(7):880–90.
  12. Krysiak R, Szkrobka W, Okopien B. The Effect of Gluten-Free Diet on Thyroid Autoimmunity in Drug-Naive Women with Hashimoto’s Thyroiditis: A Pilot Study. Exp Clin Endocrinol Diabetes Off J Ger Soc Endocrinol Ger Diabetes Assoc. 2019 Jul;127(7):417–22.
  13. Jyotsna VP, Sahoo A, Ksh SA, Sreenivas V, Gupta N. Bone mineral density in patients of Graves disease pre- & post-treatment in a predominantly vitamin D deficient population. Indian J Med Res. 2012;135:36–41.
  14. Proal AD, Albert PJ, Marshall TG. Dysregulation of the vitamin D nuclear receptor may contribute to the higher prevalence of some autoimmune diseases in women. Ann N Y Acad Sci. 2009 Sep;1173:252–9.
  15. Waterhouse JC, Perez TH, Albert PJ. Reversing bacteria-induced vitamin D receptor dysfunction is key to autoimmune disease. Ann N Y Acad Sci. 2009 Sep;1173:757–65.
  16. Vondra K, Bilek R, Matucha P, Salatova M, Vosatkova M, Starka L, et al. Vitamin D supplementation changed relationships, not levels of metabolic-hormonal parameters in autoimmune thyroiditis. Physiol Res. 2017 Sep 26;66(Supplementum 3):S409–17.
  17. Chaudhary S, Dutta D, Kumar M, Saha S, Mondal SA, Kumar A, et al. Vitamin D supplementation reduces thyroid peroxidase antibody levels in patients with autoimmune thyroid disease: An open-labeled randomized controlled trial. Indian J Endocrinol Metab. 2016 Jun;20(3):391–8.
  18. Krysiak R, Kowalcze K, Okopien B. The effect of vitamin D on thyroid autoimmunity in non-lactating women with postpartum thyroiditis. Eur J Clin Nutr. 2016 May;70(5):637–9.
  19. Gao X-R, Yu Y-G. Meta-Analysis of the Association between Vitamin D Receptor Polymorphisms and the Risk of Autoimmune Thyroid Disease. Int J Endocrinol. 2018;2018:2846943.
  20. Zhou H, Xu C, Gu M. Vitamin D receptor (VDR) gene polymorphisms and Graves’ disease: a meta-analysis. Clin Endocrinol (Oxf). 2009;70(6):938–45.
  21. Feng M, Li H, Chen S-F, Li W-F, Zhang F-B. Polymorphisms in the vitamin D receptor gene and risk of autoimmune thyroid diseases: a meta-analysis. Endocrine. 2013 Apr;43(2):318–26.
  22. Veneti S, Anagnostis P, Adamidou F, Artzouchaltzi A-M, Boboridis K, Kita M. Association between vitamin D receptor gene polymorphisms and Graves’ disease: a systematic review and meta-analysis. Endocrine. 2019;65(2):244–51.
  23. Inoue N, Watanabe M, Ishido N, Katsumata Y, Kagawa T, Hidaka Y, et al. The functional polymorphisms of VDR, GC and CYP2R1 are involved in the pathogenesis of autoimmune thyroid diseases. Clin Exp Immunol. 2014 Nov;178(2):262–9.
  24. Choi YM, Kim WG, Kim TY, Bae SJ, Kim H-K, Jang EK, et al. Low levels of serum vitamin D3 are associated with autoimmune thyroid disease inpre-menopausal women. Thyroid Off J Am Thyroid Assoc. 2014 Apr;24(4):655–61.
  25. Krysiak R, Kowalcze K, Okopień B. The effect of vitamin D on thyroid autoimmunity in euthyroid men with autoimmunethyroiditis and testosterone deficiency. Pharmacol Rep PR. 2019 Oct;71(5):798–803.
  26. Krysiak R, Kowalcze K, Okopien B. Selenomethionine potentiates the impact of vitamin D on thyroid autoimmunity in euthyroid women with Hashimoto’s thyroiditis and low vitamin D status. Pharmacol Rep PR. 2019 Apr;71(2):367–73.
  27. Dietary reference intakes for calcium and vitamin D. Washington DC: The National Academies Press; 2011.
  28. Holick MF, Binkley NC, Bischoff-Ferrari HA, Gordon CM, Hanley DA, Heaney RP, et al. Evaluation, Treatment, and Prevention of Vitamin D Deficiency: an Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab. 2011 Jul 1;96(7):1911–30.
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