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Dairou, J. Caenorhabditis elegans and Cytochromes P450. Encyclopedia. Available online: https://encyclopedia.pub/entry/20383 (accessed on 16 June 2025).
Dairou J. Caenorhabditis elegans and Cytochromes P450. Encyclopedia. Available at: https://encyclopedia.pub/entry/20383. Accessed June 16, 2025.
Dairou, Julien. "Caenorhabditis elegans and Cytochromes P450" Encyclopedia, https://encyclopedia.pub/entry/20383 (accessed June 16, 2025).
Dairou, J. (2022, March 09). Caenorhabditis elegans and Cytochromes P450. In Encyclopedia. https://encyclopedia.pub/entry/20383
Dairou, Julien. "Caenorhabditis elegans and Cytochromes P450." Encyclopedia. Web. 09 March, 2022.
Caenorhabditis elegans and Cytochromes P450
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This entry is adapted from the peer-reviewed paper 10.3390/biom12030342

The soil nematode Caenorhabditis elegans (C. elegans) is one of the simplest organisms with a laboratory model status. C. elegans is a transparent worm of about 1 mm in length, found in temperate soil environments. C. elegans is an important model used for many aspects of biological research. It is a non-infectious and non-pathogenic organism that survives by feeding on microbes such as bacteria. C. elegans was the first multicellular organism to have its whole genome sequenced and is the only organism to have its connectome (neuronal “wiring diagram”) completed. It contains 82 P450 genes, including 6 pseudogenes, divided into 16 families (13, 14, 22, 23, 25, 29, 31, 32, 33, 34, 35, 36, 37, 42, 43, 44) and 26 subfamilies in accordance with the Nelson’s nomenclature.

cytochrome P450 worm nematode predictive toxicology

1. Introduction

The soil nematode Caenorhabditis elegans (C. elegans) is one of the simplest organisms with a laboratory model status. C. elegans is a transparent worm of about 1 mm in length, found in temperate soil environments [1]. C. elegans is an important model used for many aspects of biological research [2]. It is a non-infectious and non-pathogenic organism that survives by feeding on microbes such as bacteria. With abundant food and low population density, C. elegans has a lifespan of around two or three weeks with a generation time average of three and half days. C. elegans’s developmental stages are eggs, larvae, fertile adults, and post-reproductive adults. C. elegans larvae complete development from embryo to adult with four larval stages (L1–L4) in three days. However, in an environment with limited food and/or high population density, larvae may arrest development at L2 to enter a particular stage called the dauer stage (L2d [3]). The dauer larva has a unique morphology with physiology and metabolism, which allow resistance to environmental stress. The dauer larva can live up to several months, and this stage ends when conditions favor further growth of the larva, now into the L4 stage. The dauer stage is considered to be non-aging because the post-dauer life span is not affected by the length of this stage [4]. The C. elegans model proposes the power of integrated whole animal investigations that are cost- and time-efficient and require minimal infrastructure. It is nowadays a powerful model organism not only for developmental biology but also for aging studies or toxicology [2][5]. C. elegans somatic cell locations and types, as well as networks neurons, have been mapped [6], allowing morphological evaluations of abnormalities induced by toxins and deep neurological/behavioral correlations. Furthermore, genes and signaling pathways appear to be well conserved between C. elegans and humans [7][8]. Despite their different complexity, the number of genes in C. elegans and humans is surprisingly similar (~21.000 genes in humans, ~19.000 genes in C. elegans) [9].
Contrary to toxicity tests using cell cultures, C. elegans toxicity tests provide data from a whole animal with intact and metabolically active digestive, reproductive, endocrine, sensory, and neuromuscular functions. Toxicity ranking, including LD50, in this nematode, has repeatedly been shown to be as predictive as the LD50 rankings using rats or mice [5]. However, the defense mechanisms against xenobiotics in C. elegans have been little studied, although they are essential in toxicological studies. The xenobiotic-metabolizing enzymes, and particularly P450-dependent monooxygenases (phase I enzymes), play a key role in these defense mechanisms.
P450s are ubiquitous heme-thiolate proteins involving iron-protoporphyrin IX in their active site that are widely distributed in living organisms [10][11]. The common property to all P450s discovered so far is the peculiar position of the Soret peak of their Fe(II)–CO complex around 450 nm. This redshifted Soret peak is the signature of the presence of a cysteinate ligand from the protein bound to the iron in trans position to the CO ligand [11]. Most P450s catalyze monooxygenase reactions, such as hydroxylations or epoxidations, on a great number of substrates. These reactions require the presence of dioxygen as well as a cofactor, NADPH or NADH, providing the necessary electrons via electron transfer proteins that are very often coupled to P450 inside cell membranes [11]. In the human genome, 57 genes encoding P450s have been identified (drnelson.uthsc.edu (accessed on 24 January 2022)). Some P450s catalyze oxidation steps involved in the biosynthesis and/or biodegradation of endogenous compounds such as steroids, fatty acids, and endocannabinoids [12]. On the other hand, P450s play a key role in the oxidative biotransformation of xenobiotics such as drugs, pesticides, and other environmental chemicals, facilitating their elimination from living organisms [11][12].
We know of over 62,000 bacterial P450s and 85,000 fungal P450s [13]. Since 1989, P450s have been classified according to their degree of identity in their amino acid sequence. Indeed, P450s with a degree of identity greater than 40% belong to the same family and are designated by a number behind the abbreviation P450 or CYP (CYP13, CYP14). When they have a degree of identity greater than 55%, they belong to the same subfamily and are therefore designated by a capital letter behind the number of the family (CYP13A, CYP13B). Finally, isoforms belonging to the same subfamily are differentiated by a number behind the letter of the subfamily (CYP13A1, CYP13A10). Humans have 57 functional P450s classified in 18 families and 43 subfamilies [12][14]. The largest P450s families in humans are 2, 3, and 4, and some of them are even larger in other mammals such as a mouse. As the number of families increased, it was necessary to establish a new reunification. Clans bring together families that belong to the same group (from the same ancestral gene) according to many phylogenetic trees established previously [15]. There are 11 animal P450s clans (clans 2, 3, 4, 7, 19, 20, 26, 46, 51, 74, and mitochondrial or mito). However, not all organisms own all 11 clans. For example, ecdysozoa (insects, crustaceans, nematodes including C. elegans) only have clans 2, 3, 4, and mito [15][16], whereas humans have 10 out of 11 clans, all except clan 74 [17].
The genomic sequence of the nematode C. elegans reveals over 19,000 genes, of which 76 encode for P450s [9]. Studies on this subject, having led to about a hundred articles that will be mentioned below, show that some P450s are involved in the regulation of the transition to the dauer state as well as other physiological functions of the worm. Other P450s are involved in the metabolism and bioactivation or detoxication of xenobiotics. As vertebrates, C. elegans can induce some P450s, in particular through the Aryl hydrocarbon receptor (AhR) signaling pathway. AhR is a receptor well known for its fundamental role in the metabolism of xenobiotics in vertebrates [18]. Ligands of AhR are hydrophobic xenobiotics including polycyclic aromatic hydrocarbons and halogenated compounds such as benzo (a) pyrene and 2,3,7,8-tetrachlorodibenzo-P-dioxin. AhR also has endogenous ligands such as some steroids and kynurenine. Activation of AhR leads to its translocation from the cytoplasm to the nucleus and then its heterodimerization with its partner, the aryl hydrocarbon receptor nuclear translocator (ARNT), thus forming a transcription factor. The heterodimer AhR/ARNT directly regulates the expression of many genes, including those of some enzymes involved in the metabolism of xenobiotics (P450s of family 1 and glutathione-S-transferases) [19].

2. Genetic and Phylogenetic Analysis of C. elegans P450s

C. elegans was the first multicellular organism to have its whole genome sequenced [9] and is the only organism to have its connectome (neuronal “wiring diagram”) completed [20]. It contains 82 P450 genes, including 6 pseudogenes, divided into 16 families (13, 14, 22, 23, 25, 29, 31, 32, 33, 34, 35, 36, 37, 42, 43, 44) and 26 subfamilies in accordance with the Nelson’s nomenclature [21]. Table 1 shows all the C. elegans P450s grouped by family. Almost all of the C. elegans P450s families appear to be nematode-specific [22], but they correspond to the clans 2, 3, 4, and mito also found in humans [16].
Table 1. Genes and pseudogenes (in italics) of P450s in C. elegans.
Family Transcript Transcript
Length (nt)		 Protein Protein
Length (aa)
13 T10B9.8.1 1771 CYP-13A1 519
T10B9.7.1 2258 CYP-13A2 515
T10B9.5.1 1677 CYP-13A3 520
T10B9.1.1 1655 CYP-13A4 520
T10B9.2.1 1665 CYP-13A5 520
T10B9.3.1 1759 CYP-13A6 518
T10B9.10.1 1623 CYP-13A7 518
T10B9.4.1 1594 CYP-13A8 509
T10B9.6 1423 CYP-13A9  
ZK1320.4.1 1680 CYP-13A10 519
F14F7.2.1 1653 CYP-13A11 517
F14F7.3.1 1726 CYP-13A12 518
F02C12.5.1 1652 CYP-13B1 510
K06G5.2.1 1786 CYP-13B2 511
14 K09A11.2.1 1533 CYP-14A1 491
K09A11.3.1 1535 CYP-14A2 492
K09A11.4.1 1541 CYP-14A3 498
R04D3.1.1 1549 CYP-14A4 491
F08F3.7.1 1561 CYP-14A5 492
22 T13C5.1 1999 CYP-22A1 (daf-9) 572
23 B0304.3.1 1719 CYP-23A1 534
25 C36A4.1 1631 CYP-25A1 502
C36A4.2 1570 CYP-25A2 502
C36A4.3 1775 CYP-25A3 502
C36A4.6 1677 CYP-25A4 501
F42A6.4 1506 CYP-25A5  
K06B9.1 708 CYP-25A6 236
29 C44C10.2 1515 CYP-29A1  
T19B10.1 1733 CYP-29A2 503
Y38C9B.1 1743 CYP-29A3 503
B0331.1 1682 CYP-29A4 502
31 C01F6.3 1389 CYP-31A1  
H02I12.8 1631 CYP-31A2 495
Y17G9B.3 1597 CYP-31A3 495
Y62E10A.15b2 3 1077 CYP-31A5 308
32 C26F1.2 1691 CYP-32A1 529
Y5H2B.5 1648 CYP-32B1 516
33 C12D5.7 1591 CYP-33A1 492
C25E10.21 1491 CYP-33B1 496
C45H4.21 1739 CYP-33C1 495
C45H4.17a2 1585 CYP-33C2 495
F41B5.4 1784 CYP-33C3 500
F44C8.1 1583 CYP-33C4 493
F41B5.3a.1 1568 CYP-33C5 494
F41B5.7a1 1778 CYP-33C6 494
F41B5.4 1560 CYP-33C7 494
R08F11.3 1667 CYP-33C8 494
C50H11.15 1616 CYP-33C9 496
Y49C4A.9 1738 CYP-33C11 495
Y5H2B.61 2035 CYP-33C12 426
K05D4.4 1597 CYP-33D1 492
Y17D7A.41 2 1579 CYP-33D3 495
C49C8.41 1753 CYP-33E1 494
F42A9.51 1687 CYP-33E2 494
F42A9.4 890 CYP-33E3 236
34 T10H4.10 1595 CYP-34A1 504
T10H4.11 1766 CYP-34A2 502
C41G6.1 1481 CYP-34A3  
T09H2.1.1 1680 CYP-34A4 500
B0213.10 1591 CYP-34A5 499
B0213.11 1722 CYP-34A6 498
B0213.12 1579 CYP-34A7 499
B0213.14 1584 CYP-34A8 499
B0213.15 1665 CYP-34A9 516
B0213.16 1571 CYP-34A10 499
35 C03G6.14 1546 CYP-35A1 494
C03G6.15 1588 CYP-35A2 494
K09D9.2 587 CYP-35A3 494
C49G7.8 1552 CYP-35A4 494
K07C6.5 1727 CYP-35A5 494
K07C6.4 1648 CYP-35B1 499
K07C6.3 1621 CYP-35B2 499
K07C6.2 1500 CYP-35B3 499
C06B3.3 1534 CYP-35C1 495
F14H3.10 1576 CYP-35D1 499
F14H3.13 558 CYP-35D2  
36 C34B7.3 1750 CYP-36A1 493
37 F01D5.9 1561 CYP-37A1 508
F28G4.1 1736 CYP-37B1 509
42 Y80D3A.5 1826 CYP-42A1 511
43 E03E2.1 1698 CYP-43A1 526
44 ZK177.5 1551 CYP-44A1 489
Some families have only one member (22, 23, 36, 42, 43, and 44) while others are very large, such as family 33 with 18 P450s (Table 1).
To compare the C. elegans and human P450s, a phylogenetic analysis was made. In order to achieve a phylogenetic tree, the sequences of all P450s (humans and C. elegans) were collected from the Uniprot website [23]. All trees were produced thanks to the Phylogeny site [24]: the P450 tree of C. elegans, that of human P450s, then a tree grouping together all these P450s. Subsequently, the creation of a more visual tree was carried out with the Cytoscape software [25].
The resulting phylogenetic tree common to humans and C. elegans P450s, shown in Figure 1, indicates interspecies relationships.
Figure 1. Phylogenetic tree gathering human and C. elegans P450s. The square boxes represent human P450s, while the filled round boxes represent C. elegans P450s. Different colors are assigned according to the clan to which the CYP450s belong.
Human P450s belong to 10 different clans, whereas C. elegans P450s only belong to 4 clans. Some sequences of C. elegans P450s are closer to human P450s sequences than to those of other C. elegans P450s: this is particularly the case for P450s 22A1 (clan 2, close to 17A1 and 21A2) and 44A1 (clan mito, close to 24A1). This also occurs the other way around, as for human P450 4V2 (clan 4) that is found on the tree more closely related to C. elegans P450s 29A, 32, and 37 than to other human P450s of the same family (P450s 4B1, 4F22). P450 13A10 is found in clan 3. It belongs to the same clan as P450 3A4, which is one of the most important human P450 quite often involved in the metabolism of xenobiotics [12]. Researchers also distinguish differences in the distribution of P450s in the clans between humans and C. elegans. Indeed, although clans 2 and 3 present a proportion rather like that expected considering the higher number of P450s in C. elegans than in humans, it can be  noticed that only one of the 76 P450s of the nematode has a mitochondrial sequence. The proportion is, on the other hand, very different for humans who have in this clan 7 P450s out of 57 in total. Clan 4 also has a higher percentage of human P450s (12 P450s out of 57 in total) than C. elegans P450s (13 P450s out of 76 in total). In these two species, the largest P450s clan is clan 2, whereas, in insects, clan 3 is the largest one [22].
P450s can also be classified on an even higher level into groups that contain similar P450 clans across animals, plants, and bacteria [26]. P450 families have emerged and been lost during evolution. For example, P450 51A1 (Sterol 14α-demethylase) required for one step of cholesterol synthesis is evolutionarily old and found in bacteria, plants, and humans. However, it is not found in nematodes or insects that do not synthesize cholesterol de novo. Most of the phylogenetically well-preserved P450 families typically have only one or a few members conserved across many species, whereas unstable P450 families have variable numbers of members in different species. It is believed that the stable P450 families are essential in the synthesis or degradation of endogenous substrates, while the highly diverse P450 families metabolize xenobiotics or secondary metabolites [27][28].

3. Implication of P450s in Biological Functions of C. elegans

Most of the physiological roles of P450s in C. elegans have been determined by the invalidation of P450 expression (knock-out worms or knock-out/gene silencing by RNA interference). Thus, the implication of some P450s in the biological functions of C. elegans without mention of the precise enzymatic reactions catalyzed by those P450s has been reported in several articles (Table 2).
Table 2. Implication of P450s in biological functions of C. elegans.
Biological Functions P450s Ref.
Survival/life span 22A1 [29][30][31][32][33][34][35][36]
Morphology 22A1, 33E2 [37][38][39][40][41]
Embryonic development 22A1,31A2, 31A3 [37][42][43]
Larval development 22A1, 32A1, 32B1, 33B1, 33C1, 33C2, 33D1 [35][37][42][44]
Dauer formation 13A5, 13A7, 14A1, 14A3, 22A1, 29A3, 32A1, 32B1, 33B1, 33C1, 33C2, 33D1, 33E2, 34A2, 34A5, 34A6, 34A7, 34A8, 34A9, 35B1, 35B2 [29][30][31][35][45][46][47][48][49][50]
Reproduction 31A2, 31A3, 35A2 [31][42][51][52][53]
Fat content 29A3, 31A2, 31A3, 33E2, 35A1, 35A2, 35A3, 35A4, 35A5 [42][47][48][51][53][54][55][56]
Lipid metabolism 13A12, 22A1, 29A3, 31A2, 31A3, 33E2, 35A1, 35A2, 35A4, 35A5, 37B1 [32][40][42][47][48][50][51][54][55][57][58][59][60][61]
The silencing of single P450 genes has been described to cause alterations in survival [34][35][37], life span [30][31][32][33][34][35][36], morphology [36][37][38][39][40][41][62], embryonic development [37][42][43], larval development [35][37][42][44], dauer formation [29][30][31][35][45][46][47][48][49][50], reproduction [31][42][51][52][53], fat content [42][47][48][51][53][54][55][56], or lipid metabolism [32][40][42][47][48][50][51][54][55][57][58][59][60][61].
Molecular analyses from several studies allowed a better characterization of the roles of some P450s. It was proposed that P450-dependent eicosanoids may serve as second messengers in the regulation of pharyngeal pumping and food uptake in C. elegans [63]. Some C. elegans P450s are linked to the metabolism of fatty acid-derived signaling molecules [47][55]. The silencing of P450s 31A2 and 31A3 leads to polarization and osmotic defects and to failures in meiosis and embryonic development [42]. P450s 31A2 and 31A3 also appear to be involved in the biosynthesis of lipids that are essential for the correct formation of eggshells [42]. In addition, P450 31A2 is required for sperm motility [64], and it negatively regulates the synthesis of prostaglandins [51]. Similarly, the P450s of the 35A family regulate the levels of several fatty acids and endocannabinoids in C. elegans [54]. Thus, P450s 35A2 and 35A3 are involved in the production of lipids required for eggshell formation [42]. Moreover, the deletion of P450 35A2 affects the lipid regulation and longevity of C. elegans [32]. It was also shown that the hypoxia inducible factor, HIF, regulates C. elegans stress responses and behavior via the nuclear receptor NHR-46 by targeting the gene coding for P450 36A1 [65]. This suggests that P450 36A1 is involved in the biosynthesis of a hormone ligand for this receptor.
Interestingly, there are several P450s that have been implicated in an adaptive response to changing environmental conditions. For example, Cong et al. found six P450 genes (13A8, 13A11, 14A4, 33C2, 33D3, and 35B2), whose expression levels were very low at pH 6.33, while these genes were significantly upregulated when the pH dropped to 3.13 [66].
Only a small number of reactions catalyzed by C. elegans P450s and involving endogenous substrates have been identified so far (Table 3).
Table 3. Implication of P450s in the metabolism of endogenous compounds in C. elegans.
Endobiotics Metabolite(s) Reaction(s) P450(s) Involved Ref.
EPA 17,18-epoxy-eicosatetraenoic acid Epoxidation 13A12, 29A3, 33E2 [47][55][58]
AA 19-hydroxy-AA C-H bond hydroxylation 33E2 [55]
AA 14,15-epoxy-eicosatrienoic acid Epoxidation 13A12 [58]
Cholesten-3-ones Dafachronic acids Oxidation of CH3 to COOH 22A1 (DAF-9) [57][60]
Thus, microsomes from adult worms oxidize eicosapentaenoic acid, EPA (a polyunsaturated fatty acid that acts as a precursor of prostaglandins, eicosanoids, and tromboxanes), with the main formation of 17,18-epoxy-eicosatetraenoic acid, 17,18-EEQ [47]. This oxidation is NADPH, and cytochrome P450 reductase dependent and is inhibited by usual inhibitors of mammalian arachidonic acid-metabolizing P450s. RNAi gene silencing experiments showed that P450s 29A3 and 33E2, which are related to mammalian P450 family 2, are mainly responsible for the oxidation of EPA into 17,18-EEQ [47]. Further studies using C. elegans P450 33E2 and human P450 reductase expressed in insect cells showed that P450 33E2 catalyzes the epoxygenation of EPA with the formation of 17,18-EEQ and the hydroxylation of arachidonic acid, AA, into 19-hydroxy-AA [55]. This entry also showed that P450 33E2 is expressed in the C. elegans pharynx and that 17,18-EEQ is a regulator of the C. elegans pharyngeal pumping activity. More recently, it was reported that C. elegans P450 13A12 and P450 reductase co-expressed in insect cells catalyze the epoxygenation of EPA into 17,18-EEQ and of AA into 14,15-epoxy-eicosatrienoic acid [58] (Table 3). The same article showed that 17,18-EEQ increases the C. elegans locomotion activity.
Finally, the most documented C. elegans P450 is called DAF-9 or P450 22A1, with nearly 45 articles that refer to this protein. Indeed, P450 22A1 is involved in several pathways controlling dauer formation [29], life span, and gonadal migration [31][37]. P450 22A1 catalyzes a key step in the biosynthesis of 3-keto-cholestenoic acids, also called dafachronic acids, DAs (Table 3). Those bile acid-like steroids act as ligands of the DAF-12 nuclear receptor that governs C. elegans larval development and adult longevity [37][50][67]. DAF-9 catalyzes the oxidation of a terminal methyl group of the lateral chain of cholest-4-en-3-one or cholest-7-en-3-one with the formation of delta-4- and delta-7-dafachronic acids, respectively (Figure 2) [57][60]. DAF-9 is the equivalent of P450 27A1, the human P450 that catalyzes this oxidation of a terminal methyl group of the lateral chain of 3-keto-steroids in man.
Figure 2. P450 22A1 (DAF-9) catalyzed oxidation of cholesten-3-ones into dafachronic acids.
Several redox systems are involved in the transfer of electrons from NADPH or NADH for dioxygen reduction at the P450 active site [68]. In all the above-mentioned oxidations catalyzed by microsomal type P450s, electrons from NADPH should be transferred to the P450 active site by a P450 reductase, analogous to human P450 reductase, that is encoded by the C. elegans emb-8 gene [69]. During embryonic development, emb-8 activity is essential for normal interactions between the pronucleus/centrosome complex and the posterior cortex and, thus, for proper anterior-posterior polarity. Emb-8 is also required for the formation of the secreted eggshell [69]. EMB-8 plays an important role in fatty acid modification. For instance, as mentioned in the previous paragraph, oxidation of EPA to 17,18-EEQ by adult worm microsomes is NADPH and P450 reductase dependent [47], and C. elegans P450 13A12 and P450 reductase co-expressed in insect cells catalyze the oxidation of EPA into 17,18-EEQ and of AA into 14,15-epoxy-eicosatrienoic acid [58].
The nature of the protein(s) responsible for electron transfer to the only C. elegans mitochondrial P450, P450 44A1, is less clear. In C. elegans mitochondrion, the Y62E10A.6 and Y73FBA.27 genes are coding for an adrenodoxin reductase and a ferredoxin, respectively [9]. If P450 44A1 functions as a genuine mitochondrial P450, those proteins would supply electrons to P450 44A1, even though it was argued that C. elegans lacks classical mitochondrial-type P450 [22]. More data on the nature and roles of P450 44A1 are required to answer those questions.

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