Submitted Successfully!
To reward your contribution, here is a gift for you: A free trial for our video production service.
Thank you for your contribution! You can also upload a video entry or images related to this topic.
Version Summary Created by Modification Content Size Created at Operation
1 Resistance to the gall wasp was studied by developing genetic linkage maps using a population derived from a cross between the resistant ‘Bouche de Bétizac’(C. sativa × C. crenata) and the susceptible cultivar ‘Madonna’ (C. sativa). + 1371 word(s) 1371 2020-08-18 11:33:41 |
2 format correct + 12 word(s) 1383 2020-08-24 10:25:13 |

Video Upload Options

Do you have a full video?


Are you sure to Delete?
If you have any further questions, please contact Encyclopedia Editorial Office.
Torello Marinoni, D.; Nishio, S.; Valentini, N.; Shirasawa, K.; Acquadro, A.; Portis, E.; Alma, A.; Akkak, A.; Pavese, V.; Cavalet-Giorsa, E.; et al. Chestnut Gall Wasp and Chestnut. Encyclopedia. Available online: (accessed on 17 April 2024).
Torello Marinoni D, Nishio S, Valentini N, Shirasawa K, Acquadro A, Portis E, et al. Chestnut Gall Wasp and Chestnut. Encyclopedia. Available at: Accessed April 17, 2024.
Torello Marinoni, Daniela, Sogo Nishio, Nadia Valentini, Kenta Shirasawa, Alberto Acquadro, Ezio Portis, Alberto Alma, Aziz Akkak, Vera Pavese, Emile Cavalet-Giorsa, et al. "Chestnut Gall Wasp and Chestnut" Encyclopedia, (accessed April 17, 2024).
Torello Marinoni, D., Nishio, S., Valentini, N., Shirasawa, K., Acquadro, A., Portis, E., Alma, A., Akkak, A., Pavese, V., Cavalet-Giorsa, E., & Botta, R. (2020, August 20). Chestnut Gall Wasp and Chestnut. In Encyclopedia.
Torello Marinoni, Daniela, et al. "Chestnut Gall Wasp and Chestnut." Encyclopedia. Web. 20 August, 2020.
Chestnut Gall Wasp and Chestnut

Castanea sativa is an important multipurpose species in Europe for nut and timber production as well as for its role in the landscape and in the forest ecosystem. This species has low tolerance to chestnut gall wasp (Dryocosmus kuriphilus Yasumatsu), which is a pest that was accidentally introduced into Europe in early 2000 and devastated forest and orchard trees. Resistance to the gall wasp was found in the hybrid cultivar ‘Bouche de Bétizac’ (C. sativa × C. crenata) and studied by developing genetic linkage maps using a population derived from a cross between ‘Bouche de Bétizac’ and the susceptible cultivar ‘Madonna’ (C. sativa). The high-density genetic maps were constructed using double-digest restriction site-associated DNA-seq and simple sequence repeat markers. The map of ‘Bouche de Bétizac’ consisted of 1459 loci and spanned 809.6 cM; the map of ‘Madonna’ consisted of 1089 loci and spanned 753.3 cM. In both maps, 12 linkage groups were identified. A single major QTL was recognized on the ‘Bouche de Bétizac’ map, explaining up to 67–69% of the phenotypic variance of the resistance trait (Rdk1). The Rdk1 quantitative trait loci (QTL) region included 11 scaffolds and two candidate genes putatively involved in the resistance response were identified. This study will contribute to C. sativa breeding programs and to the study of Rdk1 genes.

breeding chestnut ddRAD-seq Dryocosmus kuriphilus Yasumatsu SSR

1. Introduction

Chestnut belongs to the genus Castanea, in the Fagaceae family, which includes Quercus, Fagus, and Castanopsis. There are four major species in the genus Castanea: European chestnut (C. sativa Mill.), Japanese chestnut (C. crenata Sieb. et Zucc.), Chinese chestnut (C. mollissima Bl.), and American chestnut (C. dentata Borkh.). C. sativa is distributed along the Mediterranean basin and Asia Minor, and it is a multipurpose species not only used for nut and wood production, but also for its contribution to the landscape in mountainous areas. This species has very good nut quality, especially the ‘Marrone’ type, which is known for the fine taste and the easy-to-remove pellicle [1]. However, this species is susceptible to two main diseases, ink disease (Phytophthora cinnamomi Rands) and canker blight (Cryphonectria parasitica Murr.) [2]. In addition, most of the C. sativa cultivars are susceptible to chestnut gall wasp (Dryocosmus kuriphilus Yasumatsu).

Interspecific hybridizations have been carried out to overcome the weak points of each chestnut species. In Europe, interspecific crosses between C. sativa and C. crenata were carried out to introduce resistance genes to ink disease, canker blight, and chestnut gall wasp [2][3][4][5]. In the USA, backcross breeding was aimed at introducing blight resistance from C. mollissima into C. dentata [6]. Moreover, C. mollissima accessions were introduced in Japanese chestnut breeding programs to improve the ease of pellicle removal [7]. As these papers show, interspecific hybridization is important for chestnut breeding strategies. Therefore, constructing genetic linkage maps and accumulating genetic information among chestnut species is essential for chestnut breeding programs.


2. Chestnut Gall Wasp 

The chestnut gall wasp was first introduced from China into Japan in the 1940s and spread throughout Japan in the 1960s. C. crenata resistant cultivars, ‘Tanzawa’, ‘Tsukuba’, and ‘Ishizuchi’ were released by a public breeding program in 1959–1968. Initially, these cultivars showed total resistance to gall wasp. However, eventually, the presence of galls was found also in these cultivars, due to the appearance of new ecotypes of the insect [8]. In 1982, the parasitoid wasp Torymus sinensis Kamijo (Hymenoptera: Torymidae) was released, and a rapid decrease of the infestation was obtained [9]. To date, the control of D. kuriphilus by T. sinensis has been successful in Japan.

The chestnut gall wasp was accidentally introduced into Italy and first reported in 2002. It quickly spread to all Italian regions and later into the surrounding countries [10], causing a remarkable decrease of production (−60% in 2014 in Italy). Studies on biological control aimed at introducing the parasitoid wasp T. sinensis and at the genetic improvement for resistance to the cynipid were promptly started to solve the problem. The susceptibility to the chestnut gall wasp was evaluated in C. sativa and hybrid cultivars [11]. Out of 62 cultivars, 2 C. sativa, 1 C. crenata, and 4 hybrids between C. sativa and C. crenata showed total resistance. The resistance of the hybrid cultivar ‘Bouche de Bétizac’ was extensively studied and was found to have a simple Mendelian inheritance [3]. It was hypothesized that the mechanism of resistance involves a hypersensitive reaction in the buds [12]. The presence of H2O2 and the expression of a germin-like protein gene involved in the production of reactive oxygen compounds were revealed in infested buds of ‘Bouche de Bétizac’ at budburst.

3. Genetic Linkage Maps 

Several genetic linkage maps have been assembled for Castanea accessions. A map of C. dentata × C. mollissima was first constructed using random amplified polymorphic DNAs (RAPDs) allowing the detection of molecular markers associated with blight resistance [13]. Subsequently, C. sativa maps were built using intraspecific cross [14][15][16]. In 2013, the whole genome sequence of C. mollissima was released [17], consisting of 724.0 Mb in 41,260 scaffolds (N50, 39.6 Kb) with 91.2% coverage of estimated genome size (794 Mb). In the same year, a highly informative genetic map of C. mollissima was constructed, including 329 simple sequence repeats (SSRs) and 1064 single nucleotide polymorphisms (SNPs) markers using an expressed sequence tag database created by next-generation sequencing [18]. This consensus map consisted of 12 linkage groups ranging from 50.6 to 90.4 cM and encompassed 742.3 cM with an average distance of 0.64 cM between each pair of loci. More recent maps of C. sativa and C. crenata were constructed and anchored to the consensus map by Kubisiak et al. [18] using SNPs and anchor SSRs [4][19].

Some molecular markers associated with important agronomic traits were developed in the genus Castanea. The blight resistance genes of C. mollissima were mapped and introgressed by backcrossing into C. dentata [13][18]. The molecular markers associated with ease of pellicle removal were developed and applied in C. crenata breeding programs [19]. The quantitative trait loci (QTL) associated with agronomic traits including nut weight and pericarp splitting were identified from intraspecific crosses of C. crenata [20]. QTLs for adaptive traits, such as time of budburst, growth, and carbon isotope discrimination were identified in C. sativa [21]. In addition, QTLs for resistance to P. cinnamomi were identified in an interspecific cross progeny from C. sativa and C. crenata. However, molecular markers associated with ‘resistance to D. kuriphilus’ have not been identified yet.

The genotyping by sequencing (GBS) method [22] has illustrated a cost-effective way to identify thousands of polymorphic markers. This method is based on the construction of a library based on reducing genome complexity using restriction enzymes, to ensure sufficient read depth for polymorphism discovery. Double-digest restriction site-associated DNA-Seq (ddRAD-Seq) is a modified GBS approach that involves a two-enzyme double digestion to reduce cost and time to prepare the sequencing libraries. After the double digestion, a precise size selection is applied to exclude too short and too long fragments, resulting in greater flexibility and robustness in region recovery [23]. In silico prediction prior to actual analysis contributes to optimization of the experimental conditions for ddRAD-Seq, e.g., choices of enzymes and plant materials [24]. As the cost of next-generation sequencing (NGS) has dramatically decreased [25], more and more genetic studies involved in genetic mapping, genome-wide association mapping, and population genetics have applied the ddRAD-Seq methods [24][26][27][28][29].

4. Conclusions

Euro-Japanese F1 hybrids cultivars in Europe were obtained by INRA Bordeaux to increase the resistance of cultivated chestnuts to ink disease and canker blight. Recently, some of these cultivars showed the interesting trait of resistance to gall wasp. However, the nut organoleptic quality of the hybrid cultivars is considered much lower than that of C. sativa cultivars due to the lower quality of the Japanese chestnuts. Nevertheless, C. crenata can be seen as a major source of genes of resistance or tolerance to pests and pathogens. Once these genes are known, the acquired knowledge can be used in breeding programs. A large effect QTL, expressed across two growing seasons, was mapped on the Bouche map linkage group K and explained up to 67–69% of the phenotypic variance of the response to D. kuriphilus. A putative gene for a metacaspase-1b proteins was found in one of the scaffolds linked to the Rdk1 QTL region. The high-density maps developed in this study support further genetic studies, and once a better reference genome will be available, it will allow a more in-depth exploration of the regions flanking the trait. In addition, the obtained BC1 progeny can be used to develop molecular markers for resistance to chestnut blight and ink disease as well as for other agronomic traits, including nut quality. Further analysis on progenies from different parental lines or genome-wide association (GWAS) approaches could contribute to finding more regions of interest as well as to confirm the newly identified one.


  1. Bounous, G.; Marinoni, D.T. Chestnut: Botany, Horticulture, and Utilization. Hortic. Rev. 2010, 31, 291–347.
  2. Pereira-Lorenzo, S.; Ballester, A.; Corredoira, E.; Viéitez, A.M.; Agnanostakis, S.; Costa, R.L.; Bounous, G.; Botta, R.; Beccaro, G.L.; Kubisiak, T.L.; et al. Chestnut. In Fruit Breeding; Badenes, M.L., Byrne, D.H., Eds.; Springer: New York, NY, USA, 2012; pp. 729–769.
  3. Torello-Marinoni, D.; Nishio, S.; Portis, E.; Valentini, N.; Sartor, C.; Dini, F.; Ruffa, P.; Oglietti, S.; Martino, G.; Akkak, A.; et al. Development of a genetic linkage map for molecular breeding of chestnut. Acta Hortic. 2018, 1220, 23–28.
  4. Santos, C.; Nelson, C.D.; Zhebentyayeva, T.; Machado, H.; Gomes-Laranjo, J.; Costa, R.L. First interspecific genetic linkage map for Castanea sativa × Castanea crenata revealed QTLs for resistance to Phytophthora cinnamomi. PLoS ONE 2017, 12, e0184381.
  5. Santos, C.; Zhebentyayeva, T.; Serrazina, S.; Nelson, C.D.; Costa, R.L. Development and characterization of EST-SSR markers for mapping reaction to Phytophthora cinnamomi in Castanea spp. Sci. Hortic. 2015, 194, 181–187.
  6. Hebard, F.V. The backcross breeding program of the American Chestnut Foundation. J. Am. Chestnut Found 2006, 19, 55–77.
  7. Tanaka, K.; Kotobuki, K. Comparative Ease of Pellicle Removal among Japanese Chestnut (Castanea crenata Sieb. et Zucc.) and Chinese Chestnut (C. mollissima Blume) and Their Hybrids. J. Jpn. Soc. Hortic. Sci. 1992, 60, 811–819.
  8. Shimura, I. Chestnut breeding history. Agric. Hortic. 2003, 58, 30–32. (In Japanese)
  9. Moriya, S.; Inoue, K.; Otake, A.; Shiga, M.; Mabuchi, M. Decline of the chestnut gallwasp population, Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae) after the establishment of Torymus sinensis Kajimo (Hymenoptera: Torymidae). Appl. Entomol. Zool. 1989, 24, 231–233.
  10. Aebi, A.; Schönrogge, K.; Melika, G.; Alma, A.; Bosio, G.; Quacchia, A.; Picciau, L.; Abe, Y.; Moriya, S.; Yara, K.; et al. Parasitoid Recruitment to the Globally Invasive Chestnut Gall Wasp Dryocosmus kuriphilus. In Galling Arthropods and Their Associates; Ozaki, K., Yukwa, J., Ohgushi, T., Price, P.W., Eds.; Springer: Tokyo, Japan, 2007; pp. 103–121.
  11. Sartor, C.; Dini, F.; Marinoni, D.T.; Mellano, M.G.; Beccaro, G.L.; Alma, A.; Quacchia, A.; Botta, R. Impact of the Asian wasp Dryocosmus kuriphilus (Yasumatsu) on cultivated chestnut: Yield loss and cultivar susceptibility. Sci. Hortic. 2015, 197, 454–460.
  12. Dini, F.; Sartor, C.; Botta, R. Detection of a hypersensitive reaction in the chestnut hybrid ‘Bouche de Bétizac’ infested by Dryocosmus kuriphilus Yasumatsu. Plant Physiol. Biochem. 2012, 60, 67–73.
  13. Kubisiak, T.L.; Hebard, F.V.; Nelson, C.D.; Zhang, J.; Bernatzky, R.; Huang, H.; Anagnostakis, S.L.; Doudrick, R.L. Molecular Mapping of Resistance to Blight in an Interspecific Cross in the genus Castanea. Phytopathology 1997, 87, 751–759.
  14. Barreneche, T.; Casasoli, M.; Russell, K.; Akkak, A.; Meddour, H.; Plomion, C.; Villani, F.; Kremer, A. Comparative mapping between Quercus and Castanea using simple-sequence repeats (SSRs). Theor. Appl. Genet. 2003, 108, 558–566.
  15. Casasoli, M.; Mattioni, C.; Cherubini, M.; Villani, F. A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD, ISSR and isozyme markers. Theor. Appl. Genet. 2001, 102, 1190–1199.
  16. Casasoli, M.; Derory, J.; Morera-Dutrey, C.; Brendel, O.; Porth, I.; Guehl, J.-M.; Villani, F.; Kremer, A. Comparison of Quantitative Trait Loci for Adaptive Traits Between Oak and Chestnut Based on an Expressed Sequence Tag Consensus Map. Genetics 2005, 172, 533–546.
  17. HGW. Hardwood Genomic Project. Available online: (accessed on 20 November 2019).
  18. Kubisiak, T.L.; Nelson, C.D.; Staton, M.E.; Zhebentyayeva, T.; Smith, C.; Olukolu, B.A.; Fang, G.-C.; Hebard, F.V.; Anagnostakis, S.; Wheeler, N.; et al. A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica). Tree Genet. Genomes 2012, 9, 557–571.
  19. Nishio, S.; Takada, N.; Yamamoto, T.; Terakami, S.; Hayashi, T.; Sawamura, Y.; Saito, T. Mapping and pedigree analysis of the gene that controls the easy peel pellicle trait in Japanese chestnut (Castanea crenata Sieb. et Zucc.). Tree Genet. Genomes 2013, 9, 723–730.
  20. Nishio, S.; Terakami, S.; Matsumoto, T.; Yamamoto, T.; Takada, N.; Kato, H.; Katayose, Y.; Saito, T. Identification of QTLs for Agronomic Traits in the Japanese Chestnut (Castanea crenata Sieb. et Zucc.) Breeding. Hortic. J. 2018, 87, 43–54.
  21. Casasoli, M.; Pot, D.; Plomion, C.; Monteverdi, M.C.; Barreneche, T.; Lauteri, M.; Villani, F. Identification of QTLs affecting adaptive traits in Castanea sativa Mill. Plant Cell Environ. 2004, 27, 1088–1101.
  22. Elshire, R.J.; Glaubitz, J.C.; Sun, Q.; Poland, J.; Kawamoto, K.; Buckler, E.S.; Mitchell, S.E. A Robust, Simple Genotyping-by-Sequencing (GBS) Approach for High Diversity Species. PLoS ONE 2011, 6, e19379.
  23. Peterson, B.K.; Weber, J.N.; Kay, E.H.; Fisher, H.S.; Hoekstra, H.E. Double Digest RADseq: An Inexpensive Method for De Novo SNP Discovery and Genotyping in Model and Non-Model Species. PLoS ONE 2012, 7, e37135.
  24. Shirasawa, K.; Hirakawa, H.; Isobe, S. Analytical workflow of double-digest restriction site-associated DNA sequencing based on empirical andin silicooptimization in tomato. DNA Res. 2016, 23, 145–153.
  25. Van Dijk, E.L.; Auger, H.; Jaszczyszyn, Y.; Thermes, C. Ten years of next-generation sequencing technology. Trends Genet. 2014, 30, 418–426.
  26. Bai, B.; Wang, L.; Lee, M.; Zhang, Y.; Alfiko, Y.; Ye, B.Q.; Wan, Z.Y.; Lim, C.H.; Suwanto, A.; Chua, N.-H.; et al. Genome-wide identification of markers for selecting higher oil content in oil palm. BMC Plant Biol. 2017, 17, 93.
  27. Nagano, S.; Shirasawa, K.; Hirakawa, H.; Maeda, F.; Ishikawa, M.; Isobe, S.N. Discrimination of candidate subgenome-specific loci by linkage map construction with an S1 population of octoploid strawberry (Fragaria × Ananassa). BMC Genom. 2017, 18, 374.
  28. Yagi, M.; Shirasawa, K.; Waki, T.; Kume, T.; Isobe, S.; Tanase, K.; Yamaguchi, H. Construction of an SSR and RAD marker-based benetic linkage map for Carnation (Dianthus caryophyllus L.). Plant Mol. Biol. Rep. 2017, 35, 110–117.
  29. Zhong, Y.-J.; Zhou, Y.-Y.; Li, J.-X.; Yu, T.; Wu, T.-Q.; Luo, J.-N.; Luo, S.-B.; Huang, H. A high-density linkage map and QTL mapping of fruit-related traits in pumpkin (Cucurbita moschata Duch.). Sci. Rep. 2017, 7, 12785.
Subjects: Plant Sciences
Contributors MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to : , , , , , , , , , ,
View Times: 538
Revisions: 2 times (View History)
Update Date: 24 Aug 2020