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Aguilar Jiménez, O.; Contreras Naranjo, J.E. Electrochemical Immunosensor. Encyclopedia. Available online: (accessed on 17 June 2024).
Aguilar Jiménez O, Contreras Naranjo JE. Electrochemical Immunosensor. Encyclopedia. Available at: Accessed June 17, 2024.
Aguilar Jiménez, Oscar, Jesús Eduardo Contreras Naranjo. "Electrochemical Immunosensor" Encyclopedia, (accessed June 17, 2024).
Aguilar Jiménez, O., & Contreras Naranjo, J.E. (2021, December 01). Electrochemical Immunosensor. In Encyclopedia.
Aguilar Jiménez, Oscar and Jesús Eduardo Contreras Naranjo. "Electrochemical Immunosensor." Encyclopedia. Web. 01 December, 2021.
Electrochemical Immunosensor

Electrochemical immunosensors (EI) are systems that combine the analytical power of electrochemical techniques and the high selectivity and specificity of antibodies in a solid phase immunoassay for a target analyte.

Electrochemical immunosensors Screen Printed Electrodes Chemistry sensor

1. Introduction

The International Union of Pure and Applied Chemistry (IUPAC) defined in 2011 an electrochemical immunosensor (EI), as an integrated analytical device where the biorecognition event is based on an antigen/antibody reaction, which can transduce the product molecules of this reaction into an electric signal through the electrode surface (transducer) and quantify the amount of antigen present in the sample [[1]]. EIs can be applied in several areas of the knowledge and industry. In the last decade, the interest in building new sensor platforms that advance into commercial sensing devices for early clinical diagnosis of cancer and heart diseases has increased [[2]]. So, in this sense, a wide variety of studies about EIs have been carried out, mainly focusing on the improvement of the analytical efficiency of the device. In other words, the goal in the design and construction of sensing platforms is to measure the smallest possible amount of the target analyte present in a real complex sample with high precision and accuracy [[3]]. To achieve this goal, it is necessary to create simple and innovative sensor platforms able to suppress non-specific binding (NSB) of undesirable proteins or molecules in the electrode surface [[4]].
Various strategies have been reported for minimizing NSB and are classified in two groups: physical and chemical surface modifications. Physical modification strategies are performed attaching molecules directly to the surface, e.g., blocking buffer solutions, or by forming a complex with other particles, e.g., avidin coated surfaces. Such physical protein adsorption is governed by van der Waals forces, hydrophobic interactions, electrostatic interactions and hydrogen bonding. On the other hand, chemical modification strategies are more specific than physical adsorption and include chemical reaction between different residues (e.g., amine, thiol, carboxyl) of interacting molecules. Chemical modification strategies include: i) allotropic modification of carbon, e.g., carbon nanostructures, ii) modification by metal nanoparticles, e.g., metallic Au/Ag nanoparticles and magnetic beads, iii) polymerization, e.g., polyethylene glycol (PEG), oligo (ethylene glycol) (oEG) and conducting polymers, iv) self-assembled monolayers (SAMs), v) diazonium salt surface chemistry and vi) sol-gel chemistry modification [[5],[6]]. The efficiency of this strategy depends on the chemical properties of the sample molecules and the molecular arrangements over the electrode.

2. Electrochemical Immunosensors, EIs

According to the IUPAC, a biosensor is an analytical device that incorporates a biological element immobilized on the surface of a physicochemical transducer, which recognizes the target molecule or analyte present in a sample and, by means of the transducer, this event of biorecognition is converted into a discrete or continuous electric signal, directly proportional to the amount of analyte in the sample [[7],[8],[9],[10]]. Finally, the electrical signal is shown as a response in a computer work station or a digital display (Figure 1). In general, biosensors are classified by their biorecognition element or their transduction principle [[11],[12]]. Antibodies, enzymes, cells/tissues, nucleic acids and aptamers are some of the elements of biorecognition frequently used. The principal types of transducers are: optical [[13],[14]], electrochemical [[15],[16],[17]], calorimetric [[18],[19]] and piezoelectric [[20],[21]].
Figure 1. Components of a biosensor [[2]].
The most outstanding characteristics of these devices, which make them highly attractive options as analytical tools, are: their selectivity and specificity, high sensitivity, responsiveness that leads to a short analysis time, their ability to be included in integrated systems, ease of automation, ability to work in real time, its versatility and low cost, among others [[22],[23]]. In the EI, an affinity biosensor, the immobilized biological entities are antibodies (Abs) that recognize the target analyte (antigen, Ag), while an electrochemical transducer converts the binding event between the antigen and antibody, resulting in the formation of the complex antigen-antibody (Ag-Ab), into a useful electrical signal, either an electrical current (amperometric immunosensors), a potential difference (potentiometric immunosensors) or a change in resistivity (conductimetric and impedimetric immunosensors) [[24],[25]].
Antibodies, specifically monoclonal antibodies, are a very useful biorecognition element in EIs due to the high affinity and specificity that they show for the analyte of interest, additionally, they show good stability and versatility and a relative low cost compared to other elements of biorecognition such as polyclonal antibodies, enzymes, whole cells, nucleic acids and molecularly imprinted polymers (MIPs) [[26],[27]]. Monoclonal antibodies present some advantages compared to polyclonal antibodies., among these, its high specificity for a single epitope in a multivalent antigen, contrary to what happens in polyclonal antibodies that recognize and bind to the same antigen but can be combined with different epitopes. Another advantage of monoclonal antibodies is that the hybridoma cell line that produces them is potentially "immortal" and can produce the same antibodies in a constant and indefinite manner [[28],[29]].
EIs combine the high sensitivity of electrochemical techniques and the high selectivity and specificity of antibodies to the target analyte. The most common type of amperometric immunosensors is the ELISA sandwich immunoassay with electrochemical detection. Usually, a sandwich immuno-complex (BtnAb-Ag-AbHRP) is composed of a biotinylated primary antibody, also called capture antibody (BtnAb), the analyte, also called antigen (Ag) and a second antibody (detection antibody) labelled with a redox enzyme, e.g., horseradish peroxidase, HRP (AbHRP) adsorbed onto an avidin-coated electrode surface due to the high affinity of the avidin-biotin system (or its analogous streptavidin and neutravidin).
The purpose of this test is to quantify the number of antigens (target analyte) present in complex samples such as human serum through the detection of the immuno-complex, via an electron transfer reaction between the redox enzyme, the enzyme substrate (S), e.g., hydrogen peroxide (H2O2), and the redox mediator, e.g., 3,3′,5,5′-tetramethylbenzidine (TMB). The HRP oxidize the TMB into TMB+ in the presence of H2O2, releasing one electron per mole of TMB oxidized. TMB+ is reduced to TMB by the imposition of a potential. Thus, as a consequence of the oxidation and reduction of the electroactive product (P) in the electrode surface, a steady-state current is established in the process (Figure 2). If the BtnAb-Ag-AbHRP complex does not form, the measurable current values will be considerably low, which means a negative result. In contrast, high current values indicate a positive result [[30],[31],[32],[33]].
Figure 2. Basic analytical principle of an ELISA sandwich amperometric immunosensor using screen printed carbon electrodes (SPCEs) [[32]].
An example of an electrochemical ELISA sandwich immunoassay was proposed by Zhang et al. in 2015 for the quantification of prostate-specific antigen (PSA) using redox and catalysis “all-in-one” infinite coordination polymer (PtNP@ICP) as a signal tag for the label of PSA on the polyamidoamine dendrimers-modified glassy carbon electrode interface. Their results showed a limit of detection (LOD) of 0.3 pg/mL at 3sB [[34]].
As it is known, EIs are based on the antibody-antigen interaction and possess high specificity and selectivity for the analyte of interest. Since the antibody is immobilized on small electrode surfaces, this type of biosensors can be miniaturized and easily converted into portable devices with the help of microelectrodes (where the immunological reaction will be carried out), among which SPEs are of great importance [[29],[30]]. Immunosensors have a wide variety of applications (e.g., biomedical, clinical diagnosis, environmental and food industry) compared to enzyme-based sensors, given that antibodies are universal in contrast to enzymes, which depend on each substrate [[35]].
The advantages that EIs present over immunoassays, specifically in relation to the commercial sandwich ELISA test, are their good portability (easy miniaturization) in order to carry out clinical diagnosis in the place where the patient is located (point of care testing, POCT) with a minimum of training required, good sensitivity, low cost, rapid analysis, instrumental simplicity and precise measurements, while maintaining the optical approximation of the ELISA test. On the other hand, adaptation of the ELISA assay to an electrochemical approach is not an easy task, because many factors can affect the signal response and hence the performance analysis regarding sensitivity. A poor signal response may be due to the denaturation or loss of affinity and specificity of antibodies and proteins when they are adsorbed onto the electrode surface, an incorrect orientation of antibodies in its adsorbed state which leads to an increase of the steric hindrance, and cross-reactivity from antibodies to other non-specific molecules present in the sample instead of the antigen of interest. Also, in EIs, it is desirable to generate the enzymatic product directly on the electrode surface to favor the electron transfer rate; moreover, direct adsorption of interacting biomolecules may passivate or poison the electrode surface, thus affecting the electrochemical behavior of the sensor. These are the most common concerns in EIs in order to achieve performances comparable or even better than those of the commercial ELISA optical approach [[36],[37],[38],[39]].
Methodologies oriented to improve the electrochemical detection of biomolecules of interest in the development of more sensitive platforms employ magnetic beads, nanostructures (carbon nanotubes, CNTs, multiwall carbon nanotubes MWCNTs, Quantum dots), and nanoparticle labels [[40],[41],[42],[43]].

3. Screen Printed Electrodes, SPEs

A wide variety of electrodes have been used as a support in the fabrication of immunosensor devices: carbon paste electrodes, glassy carbon electrodes and gold electrodes. Recently, most of the sensor devices have been constructed onto SPEs. Nowadays, the screen printing microfabrication technology is well stablished, offers high-mass production of solid, planar, small size, low cost, disposable, mechanically robust and highly reliable thick film electrodes; allowing the construction of portable, low cost and pocket size devices for on-site diagnosis [[44],[45]]. A detailed description of the fabrication process of SPEs was reported by Li et al. in 2012 [[46]].
In summary, fabrication of SPEs consists of various steps: selection of the screen or mesh which will define the thick layer film, geometry and size of the SPE, selection and preparation of the inks for the reference, counter and working electrode, selection of the substrate, sequential deposition of layers of ink onto the substrate, drying and curing steps between each layer deposition of the ink [[47],[48],[49]]. Even though the exact formulation of the inks is according to the manufacturer as copyright information, it is well stablished that the ink is made mainly by synthetic grade graphite, vinyl or epoxy-based polymeric binder and solvents. Graphite is the electrode material, the binder increases adhesion and mechanical strength, and solvents allow to control the ink viscosity [[50]]. The control of the ink composition is very important, because any minimal change strongly affects the electron transfer process and changes the overall performance of the sensor device [[51],[52]].
The SPE substrate usually is a non-conducting solid surface material such as alumina, glass, ceramic, plastic, etc. and the electrode conducting parts are made of carbon ink/paste, platinum, gold or other metal pastes. The improvement of the EIs is intimately related to the material of the working electrode on which the reactions of interest occurs. Carbon or other forms of carbon such as graphene, graphite, fullerene, carbon nanotubes, and single/multi-wall carbon nanotubes are most commonly employed as a material in the fabrication of the working electrode. Some characteristics that made carbon suitable for this purpose are its low cost (compare to metals e.g., gold, silver or platinum; which have been also utilized as a working electrode materials and present higher fabrication costs), easiness to modify, chemically inert, good electrical and thermal conductivity, high mechanical and dimensional stability and for electrochemical activities its low background currents and wide potential operational window [[53],[54],[55]]. Usually, the material of the reference electrode is Ag/AgCl and the counter electrode generally is made from the same working electrode material [[54],[55]] (Figure 3).
Figure 3. Design of an easy-to-use, disposable and portable SPCE [[52],[54]].
A diversity of SPEs configurations are available nowadays in the market for a broad spectrum of applications which can be personalized according to the customer requirement. Configurations of two electrodes (working and reference electrodes, also called first generation SPEs), three electrodes (working, reference and auxiliary electrodes, also called second generation SPEs) are the most used [[56]]. In addition, SPEs with multiplex working electrodes, arrays of eight SPEs and of 96 SPEs in a 96-well plate have been developed for the simultaneous detection of multiple biomarkers [[57]].
Recently, the interest to replace rigid substrates with paper substrates for fabricating SPEs has strongly increased in the development of new electroanalytical tools. Various studies about SPEs based on paper and transparency in order to detect a broad spectrum of biomolecules have been reported [[58],[59]]. Further, wearable, tattoo-based wearable and skin worn electrochemical sensors and biosensors employing SPEs as a sensor platform are a very good alternative to measure in real time physical parameters such as heart rate, respiration rate, oxygenation of the blood, skin temperature, bodily motion, brain activity, and blood pressure, etc., without compromising user comfort. These are some examples of the great versatility of SPEs in the continuous development of bioanalytical devices [[60],[61]].


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