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Soyyilmaz, B. Human Milk Oligosaccharides (HMOs). Encyclopedia. Available online: (accessed on 14 June 2024).
Soyyilmaz B. Human Milk Oligosaccharides (HMOs). Encyclopedia. Available at: Accessed June 14, 2024.
Soyyilmaz, Buket. "Human Milk Oligosaccharides (HMOs)" Encyclopedia, (accessed June 14, 2024).
Soyyilmaz, B. (2021, August 23). Human Milk Oligosaccharides (HMOs). In Encyclopedia.
Soyyilmaz, Buket. "Human Milk Oligosaccharides (HMOs)." Encyclopedia. Web. 23 August, 2021.
Human Milk Oligosaccharides (HMOs)

Human milk oligosaccharides (HMOs) are non-digestible and structurally diverse complex carbohydrates that are highly abundant in human milk. To date, more than 200 different HMO structures have been identified. Their concentrations in human milk vary according to various factors such as lactation period, mother’s genetic secretor status, and length of gestation (term or preterm).

human milk oligosaccharides human milk carbohydrates HMO composition non-digestible carbohydrates 2′-fucosyllactose

1. Introduction

Breastfeeding has been associated with lower rates of infectious diseases and infantile mortality, reduced risk for obesity, cardiovascular disease, inflammatory bowel disease (IBS), and type II diabetes [1][2][3]. In addition to the milk macronutrients such as lactose, lipids, and proteins, human milk contains complex carbohydrate structures known as human milk oligosaccharides (HMOs), which attract significant attention since they constitute the largest remaining compositional difference between breastmilk and infant formula.

HMOs constitute the third most abundant solid component of human milk, exceeding the amount of protein [4]. Compared to the milk oligosaccharide fraction of human milk, some remarkable differences are observed in the milk oligosaccharide fraction of other mammals: (a) the total amount of milk oligosaccharides are typically significantly lower [5][6], particularly for domestic farmed animals [7]; (b) the structural complexity and bias of individual structures is lower and different [8][9][10]; and (c) different carbohydrate epitopes are detected [11][12]. In addition to their unique abundance in human milk, there is evidence that HMOs are present in the maternal serum during early gestation, in the umbilical cord blood, and in amniotic fluid [13][14][15].

HMOs are largely indigestible by the infant, hence do not function as direct energy resources for the infant. The majority reach the colon where they are utilized by specific infant gut bacteria and approximately 1% is absorbed [16][17]. The HMO profile of human milk appears fundamental for shaping the gut microbiota of the infant by selectively stimulating the growth of specific bacteria, especially bifidobacteria [18]. The bifidogenic effects found in breastfed infants include proliferation of specific bifidobacteria such as Bifidobacterium infantisB. breve, and B. bifidum, whose genomes encode a large proportion of oligosaccharide processing and transporting genes clustered within conserved loci [19][20][21][22]. The ability of these bifidobacterial species to utilize HMOs implies a co-evolution, where the glycans produced by the host have served as a carbon and energy source for these bifidobacterial species [23]. Bifidobacterial abundance have been linked to host protection from pathogenic bacteria by helping prepare the mucosal immune system, consequently decreasing susceptibility to various diseases later in life [24]. In addition to their bifidogenic activity, HMOs have also shown to be able to directly or indirectly affect mucosal and systemic immunity, help reduce the risk of pathogenic infection and may support brain development in infants [25][26][27][28][29].

2. Structures and Abbreviations of HMOs

All HMOs derive from lactose (galactosyl-β1-4-glucose) and the HMO fraction of human milk is characterized by extension with four monosaccharides: N-acetyl-D-glucosamine (GlcNAc), D-galactose (Gal), sialic acid (Neu5Ac), and/or L-fucose (Fuc). GlcNAc and galactose are added in specific order and linkages to form the neutral-core structures. While Neu5Ac and Fuc can be present on the terminal positions of either lactose or the core structures, forming sialylated and fucosylated groups. The resulting composition of the HMO fraction is complex and diverse with more than 200 different HMO structures detected by sensitive mass spectrometry [30][31][32]. Names, abbreviations, and structures of the most abundant HMOs reported are listed in Table 1. The collective body of analytical data shows that HMOs can be classified into three fundamental structure classes: (1) neutral-core HMOs (containing GlcNAc); (2) neutral fucosylated HMOs (containing fucose); and (3) acidic HMOs (acidic fucosylated and acidic nonfucosylated) (containing sialic acid). Table 2 lists the 15 most abundant structures. For further information on the chemical structures of individual HMOs, it is recommended to refer to Chen et al. (2015), who provide an elaborate resource to discover the structures of HMOs [32].

Table 1. Structures of the 15 most abundant HMOs in mature human milk.

Abbreviation Name Structure Abbreviation Name Structure
Neutral HMOs (neutral core and neutral fucosylated) Acidic non-fucosylated HMOs
LNT Lacto-N-tetraose Nutrients 13 02737 i001 3′-SL 3′-Sialyllactose Nutrients 13 02737 i002
LNnT Lacto-N-neotetraose Nutrients 13 02737 i003 6′-SL 6′-Sialyllactose Nutrients 13 02737 i004
2′-FL 2′-Fucosyllactose Nutrients 13 02737 i005 DSLNT Disialyllacto-N-tetraose Nutrients 13 02737 i006
3-FL 3-Fucosyllactose Nutrients 13 02737 i007 LST c Sialyllacto-N-neotetraose c Nutrients 13 02737 i008
DFL (LDFT) Difucosyllactose Nutrients 13 02737 i009   Acidic fucosylated HMOs  
LNDFH-I (DFLNT) Lacto-N-difucohexaose I Nutrients 13 02737 i010 FDS-LNH-I Fucosyldisialyllacto-N-hexaose I Nutrients 13 02737 i011
LNFP-I Lacto-N-fucopentaose I Nutrients 13 02737 i012 Nutrients 13 02737 i014
LNFP-II Lacto-N-fucopentaose II Nutrients 13 02737 i013
LNFP-III Lacto-N-fucopentaose III Nutrients 13 02737 i015
TF-LNH Trifucosyllacto-N-hexaose Nutrients 13 02737 i016

Table 2. Distribution of mothers’ phenotypes and corresponding milk groups.

Secretor Status Secretor Non-Secretor Secretor Non-Secretor
Milk group 1 2 3 4
Milk Phenotype Se+/Le(a−b+) Se−/Le(a+b−) Se+/Le(a−b−) Se−/Le(a−b−)
α1,2-fucosylated HMOs
(FUT2 enzyme 1)
+ +
α1,3-fucosylated HMOs (FUT3, FUT5, FUT6 enzymes) + + + +
α1,4-fucosylated HMOs (FUT3 enzyme) + +
Typical frequency in global population 70% 20% 9% 1%

1 Group 1 (secretor) mothers express both FUT2 and FUT3. Group 2 (non-secretor) mothers express FUT3 but not FUT2. Group 3 (secretor) mothers express FUT2 but not FUT3. Group 4 (non-secretor) mothers express neither FUT2 nor FUT3 [33]. FUT = fucosyltransferase.

3. Factors Influencing HMO Variability

The composition and concentration of individual HMO structures in human milk vary according to genetic and non-genetic factors. Even though neither carbohydrate nor oligosaccharide synthesis is directly genetically encoded (unlike DNA, RNA, and proteins), HMO variability is strongly dependent on the activity of two specific enzymes that are encoded by the Secretor (Se) and Lewis (Le) genes in the mother [27][29]Se and Le genes encode the enzymes α1-2-fucosyltransferase (FUT2) and α1-3/4-fucosyltransferase (FUT3) respectively, both affecting the biosynthesis of fucosylated HMOs [27]. Milk from women with inactivated FUT2—due to a diversity of different mutations in both alleles of the Se gene—contain zero or only traces of the α1-2-fucosylated HMOs. Similarly, FUT3 activity can be inactivated (or severely reduced) due to a diversity of different mutations in both alleles of the Le gene, thus Lewis-negative women’s milk contain zero or only traces of the α1-4-fucosylated HMOs (α1-3-fucosylation is encoded by several enzymes and is therefore not eliminated by FUT3 deactivation). Therefore, mothers who carry the Se gene express FUT2 enzyme while mothers who do not carry Se do not express the FUT2 enzyme. The interplay of the FUT2 and FUT3 enzymes leads to two main types of Lewis antigens (Lea and Leb) and four common milk phenotypes are observed: Se+/Le(a−b+), Se-/Le(a+b−), Se+/Le(a−b−), Se−/Le(a−b−). Reflecting these phenotypic differences, HMO profiles in lactating mothers are classified into four milk phenotypes, or four different ‘milk groups’ resulting in distinct structural features in their oligosaccharide fraction (Table 1) [33][34]. There are also different consequences of each milk group for the infant postulated. For instance, moderate-to-severe and calicivirus-associated diarrhea occurred less often in infants whose milk contained high levels of 2-linked fucosylated HMOs, suggesting that HMO profile is clinically relevant for incidence of diarrhea [35].

The highest HMO concentrations are generally found in colostrum (first milk). Other than lactation period, the non-genetic factors contributing to the quantitative and structural variability of HMOs among mothers are still to be unraveled. There is evidence demonstrating HMO variability across different populations [36][37] which is possibly explained in large part by irregular distribution of milk groups geographically [38]. Non-genetic factors that might contribute to variation in HMO contents are mother’s age, nutritional status, weight, body-mass index, and significant health issues of the mother [39].

In addition to natural causes described above (particularly genetic secretor status and lactation period) differences in reported HMO concentration are certainly also linked to varying inter-laboratory methodologies for glycan analysis [40]. Nevertheless, existing literature of HMO quantification represents a useful resource to determine the mean concentrations of individual HMO structures, and investigate their variation over the course of lactation. A previous systematic review by Thurl et al., provides knowledge on HMO amounts in human milk from mothers of known secretor status [41]. To date, no review has been performed to determine more robustly the most abundant HMOs observed globally in term human milk throughout lactation and their representative mean concentrations. In the present review, each included publication was regarded as an observational point with the central aim to make an estimation of the global HMO profile for pooled samples regardless of the individual variations caused by genetic and non-genetic factors. The authors decided on determining an overview on pooled milk samples instead of secretor and non-secretor samples given that the chosen objective was to determine a ranking of individual HMOs by global means across different milk phenotypes, regardless of individual variations and has included all relevant and reliable HMO concentration data identified.


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