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Marine biodiversity is threatened by several anthropogenic pressures. Pollution deriving from the discharge of chemical contaminants in the sea represents one of the main threats to the marine environment, influencing the health of organisms, their ability to recover their homeostatic status, and in turn endangering biodiversity. Molecular and cellular responses to chemical pollutants, known as biomarkers, are effect-based methodologies useful for detecting exposure and for assessing the effects of pollutants on biota in environmental monitoring. Pollution biomarkers can be useful tools for monitoring and assessment of pollution threats to marine biodiversity, both in the environmental quality monitoring of protected areas and the assessment of the health status of species at risk.
Chemical pollution derived from the discharge of chemical contaminants in the sea, from both point and non-point pollution sources, represents one of the main threats to the marine environments and their resources and services and remains a great environmental challenge [1][2]. Shipping is a source of pollutants through accidental spillages, operational discharges, and antifouling paint leaching; mariculture accounts for medicinal product, biocide, and food additive release; offshore activities produce drill cuttings and hydrocarbon release; dredging of sediment and dumping at sea contribute to water column contaminant level increase [3]. Moreover, the release of chemical contaminants into the sea from land-based activities, such as urban wastewater discharge, industrial and agricultural activities, mining, and runoff from coastal areas, contributes dramatically to the contamination of the seas. Several of them are contaminants of emerging concern (CECs), which include a wide array of anthropogenic chemicals that have no regulatory standards yet [4][5].
Chemicals absorbed by the organisms through the gills, the gastrointestinal tract, and the tegument can interact with biological macromolecules, producing several toxicological effects at the cellular and molecular levels. This includes enzyme inhibition, alterations of transport properties, alteration of the functioning of membrane and intracellular receptors, alterations of intracellular signaling pathways, oxidative stress, and DNA damage [6][7][8][9][10]. These primary effects at the molecular and cellular levels can produce integrated toxicity effects over time, including impairment of organ and systems functioning such as neurotoxic effects, immunological responses, hepatotoxicity, behavioral changes, reproductive and developmental alterations, endocrine disruption, and genotoxicity [2]. For example, some persistent marine pollutants can exacerbate the adverse effects of certain pesticides as well as other persistent organic pollutants (POPs) in marine organisms [11].
Healthy oceans are among the main objectives of the EU by 2030. To reach these goals, water quality monitoring and assessment assume a fundamental role. The study of the molecular, cellular, and physiological alterations in the organism in relation to the exposure to chemical pollutants has contributed to developing several markers (biomarkers) of exposure and toxicological responses to chemical pollutants [12][13]. The application of the biomarker approach in marine environment monitoring and assessment, integrated into the physicochemical analysis of the environmental matrices, has greatly increased in recent years. This is mainly due to the fact that the assessment of the entity of the organism exposure to pollutants in a certain environment and the extent of the suffered toxicological effects is of fundamental importance for decision making related to habitat and species protection, ecosystem services provision, adoption of remediation procedures, or impacted area monitoring [14].
Recently, the application of the biomarker approach in biomonitoring is considered with great interest in the field of biodiversity conservations. Considering that chemical pollution is recognized as one of the major pressures driving biodiversity reduction loss worldwide [15], the study of the responses of the organisms to the anthropogenic alterations of the environment that may cause or contribute to population decline can support biodiversity conservation strategies. Biomarkers have been recently applied to several research areas of the biodiversity conservation field, including environmental quality monitoring of protected areas and the assessment of the health status of species at risk.
Protected Areas | Bioindicator Species | Bioindicator Class | Endpoint | Biomarkers Analyzed | Ref. |
---|---|---|---|---|---|
Europe | |||||
Egadi Islands Marine Protected Area (Italy) |
Coris julis, Patella caerulea, Paracentrotus lividus |
Osteichthyes Gastropoda Echinoidea |
Detoxification of organic pollutants | EthoxyresorufinO-deethylase, BaPMO, NADH ferry red, and NADH cyt c | [23] |
Tremiti Islands Marine Protected Area (Italy) | Paracentrotus lividus | Echinoidea | Coelomocytes alterations | Coelomocytes subpopulations ratio, heat-shock protein 70 | [24] |
National Park of La Maddalena Arcipelago (Italy) | Mytilus galloprovincialis | Bivalvia | Lysosomal alterations | Lysosomal membrane stability, lipofuscin content, neutral lipid contents, lysosomal structural changes | [25] |
Capo Peloro Natural Reserve (Italy) | Atherina boyeri | Osteichthyes | Detoxification of organic pollutants, neurotoxicity, genotoxicity |
Acetylcholinesterase, benzo(a)pyrene-monooxygenase, polycyclic aromatic hydrocarbons metabolites in bile, erythrocytic nuclear abnormalities assay |
[26] |
The Pelagos Sanctuary (International Sanctuary for the Protection of Mediterranean Marine Mammals) (Italy, France) |
Meganyctiphanes norvegica | Malacostraca | Detoxification of organic pollutants, neurotoxicity, response to xenoestrogens | Cytochrome P450, BaPMO activity, NADPH cytochromec reductase, NADH-ferricyanide reductase, esterases, porphyrins, vitellogenin, zona radiata proteins, acetylcholinesterase |
[27] |
Stenella coeruleoalba | Mammalia | Detoxification of organic pollutants, oxidative stress |
Cytochrome P4501A, cytochrome P4502B, catalase | [28] | |
Balaenoptera physalus | Mammalia | Detoxification of organic chemical pollutants, oxidative stress |
Cytochrome P4501A, cytochrome P4502B, lipoperoxidation | [29] | |
Balaenoptera physalus, Physeter macrocephalus |
Mammalia | Metal excretion | Metals in the fecal material | [30] | |
North America | |||||
Florida Keys National Marine Sanctuary (U.S.A.) |
Montastraea annularis | Anthozoa | Oxidative stress, stress protein multidrug resistance induction |
Superoxide dismutase, glutathione peroxidase, glutathione-s-transferase, heat-shock proteins, metabolic condition, multixenobiotic resistance proteins | [31] |
Veracruz Coral Reef System National Park (Mexico) | Haemulon Aurolineatum, Ocyurus chrysurus |
Osteichthyes | Detoxification of organic chemical pollutants, response to xenoestrogens | Cytochrome P4501A, vitellogenin, glutathione-S-transferase, PAH metabolites in fish bile |
[32] |
Natural protected area of Laguna Madre in the Gulf of Mexico (Mexico) | Chione elevata | Bivalvia | Neurotoxic effects, oxidative stress, metabolic alterations |
Acetylcholinesterase, butyrylcholinesterase, carboxylesterase, alkaline phosphatase, glutathione s-transferase, oxygen radical absorbance capacity |
[33] |
South America | |||||
Morrocoy National Park (Venezuela) | Siderastrea sidereal | Anthozoa | Detoxification of organic chemical pollutants, oxidative stress | Cytochrome P450 I, cytochrome P450 II, NADPH reductase, glutathione S-transferase, catalase, superoxide dismutase | [34] |
Parque Nacional Archipielago Los Roques (Venezuela) | Siderastrea sidereal | Anthozoa | Detoxification of organic chemical pollutants, oxidative stress | Cytochrome P450 I, cytochrome P450 II, NADPH reductase, glutathione S-transferase, catalase, superoxide dismutase | [34] |
Fernando de Noronha Archipelago protected area (Brazil) | Amphistegina lessonii | Foraminifera | Oxidative stress, metal detoxification | Antioxidant capacity against peroxyl radicals, lipid peroxidation, protein carbonylation, metallothionein-like proteins | [35] |
Paranaguá Bay protected areas (Brazil) | Atherinella brasiliensis | Osteichthyes | Neurotoxicity, detoxification of organic chemical pollutants, oxidative stress | Cholinesterase, ethoxyresorufinO-deethylase, glutathione S-transferase, catalase | [36] |
Cananéia–Iguape–Peruíbe Environmental Protected Area (Brazil) | Cathorops spixii | Osteichthyes | Detoxification of organic pollutants, oxidative stress, genotoxicity, metal detoxification | Glutathione S-transferase, glutathione peroxidase, GSH levels, lipid peroxidation, DNA strand breaks, metallothonein |
[37] |
Cathorops spixii | Osteichthyes | Genotoxicity | Comet assay, micronucleus test (MN), and nuclear abnormalities test (NA) in peripheral blood |
[38] | |
Natural Protected Area San Antonio Bay (Argentina) | Neohelice granulata | Malacostraca | Detoxification of organic pollutants, oxidative stress, metal detoxification | Catalase, lipid radical content, lipid peroxidation, α-tocopherol, catalase, glutathione-S-transferases, metallothioneins | [39] |
Cananéia–Iguape–Peruíbe Protected Area (Brazil) | Callinectes danae | Malacostraca | Genotoxicity, detoxification of organic pollutants, oxidative stress, metal detoxification, neurotoxicity | Glutathione S-transferase, glutathione peroxidase, intracellular glutathione, acetylcholinesterase, lipid peroxidation, metallothionein, DNA strand breaks |
[40] |
Estuarine Lagoon Complex of Iguape–Cananéia (Brazil) | Gobioides broussonnetii | Osteichthyes | Oxidative stress, genotoxicity, metal detoxification, histopathological alterations |
Superoxide dismutase, catalase, glutathione peroxidase activity, glutathione S-transferase, glutathione, metallothionein, lipoperoxidation, micronuclei, histological alterations | [41] |
Australia | |||||
Great Barrier Reef (Australia) | Plectropomus leopardus | Osteichthyes | Detoxification of organic chemical pollutants, neurotoxicity | EROD, cholinesterase | [42] |
Acropora millepora | Anthozoa | Oxidative stress | Genetic loci involved in environmental stress tolerance and antioxidant capacity | [43] |