Submitted Successfully!
Thank you for your contribution! You can also upload a video entry or images related to this topic.
Ver. Summary Created by Modification Content Size Created at Operation
1 + 3309 word(s) 3309 2021-07-07 08:58:28 |
2 format correct Meta information modification 3309 2021-07-12 04:35:31 |

Video Upload Options

Do you have a full video?


Are you sure to Delete?
If you have any further questions, please contact Encyclopedia Editorial Office.
Savina, I.N. Cryotropic Gelation and Macroporous Scaffolds. Encyclopedia. Available online: (accessed on 11 December 2023).
Savina IN. Cryotropic Gelation and Macroporous Scaffolds. Encyclopedia. Available at: Accessed December 11, 2023.
Savina, Irina N.. "Cryotropic Gelation and Macroporous Scaffolds" Encyclopedia, (accessed December 11, 2023).
Savina, I.N.(2021, July 10). Cryotropic Gelation and Macroporous Scaffolds. In Encyclopedia.
Savina, Irina N.. "Cryotropic Gelation and Macroporous Scaffolds." Encyclopedia. Web. 10 July, 2021.
Cryotropic Gelation and Macroporous Scaffolds

Cryogels obtained by the cryotropic gelation process are macroporous hydrogels with a well-developed system of interconnected pores and shape memory. Biodegradable cryogels have been prepared from natural polymers (biopolymers) and synthetic polymers with biodegradable/bioresponsive bonds containing bio-resembling or artificial units. The cryogels could be composed of one type of polymer or a combination of different materials. Polymers from natural origins (plants or animals) attract considerable attention for their intrinsic biocompatibility and potential ability to mimic ECM, favoring cell-matrix interactions for tissue-engineering and regeneration.

macroporous hydrogels cryogels natural and synthetic polymers tissue engineering biomaterials

1. Cryotropic Gelation

Cryotropic gelation is the formation of a hydrogel under semi-frozen conditions, when most of the solvent crystallizes, and gelation occurs in small non-frozen areas around the solvent crystals (Figure 1) [1][2][3]. The solvent crystals perform as a template for the pores, and after melting, they leave large voids filled with liquid solvent [2]. The most common solvent used is water, but other solvents or solvent mixtures with freezing points reasonably close to zero can be used, such as dimethyl sulfoxide (DMSO), benzene or cyclohexane [4][5]. Water is considered to be the best solvent for the formation of biocompatible cryogels for biomedical applications. Gelation can occur through different mechanisms, usually through the formation of covalent bonds, or physical interactions (ionic bonding, hydrogen bonding and others) [6]. Synthesis by radical polymerization is the most commonly used method of preparing polymer cryogels. Acrylate derivatives are mostly used as gel-forming units together with an ammonium persulphate and tetramethylethylenediamine (APS/TEMED) initiating system in these reactions. Cryogels based on natural polymers can be prepared by cross-linking intact or pre-modified polymers utilizing the amino, carboxyl, hydroxyl and other functional groups. Variation of the cryotropic gelation parameters, such as the freezing temperature, cooling rate, and the presence of the ions or other solutes, as well as the polymer and solvent content, allows the tuning of cryogel properties for a specific application.
Figure 1. Scheme of preparation of cryogels: (i) initial system (the gel precursor solution is cooled down to a temperature below freezing point); (ii) frozen system (the ice crystals formed and solutes are concentrated in non-frozen zones); (iii) intermediate system (gel is formed in non-frozen liquid around ice crystals); (iv) cryogel is formed upon defrosting.

2. Cross-Linking

Cross-linking is one of the major factors that affect the mechanical and biological properties of cryogel scaffolds and their biodegradability. The types of cross-linking are summarized in Table 1. Although cryogel scaffolds can be readily formed through physical cross-linking—for example, involving ionic interaction and hydrogen bonding—they are generally reversible, and the resultant porosity and mechanical properties will not always be satisfactory for all applications. Poly(vinyl alcohol) (PVA) is one of the most studied synthetic polymers that can be physically cross-linked to obtain cryogels [1][7]. PVA cryogels are formed as a result of the formation of microcrystalline zones and are thermally reversible. The pore size of PVA cryogels is less than 10 µm. They have been used in a number of biotechnological applications for the immobilization of enzymes [8] and cells [9], as well as in tissue engineering, mainly for mimicking bone and cartilage tissues [10][11].
Table 1. Types of the cross-linking reactions for the formation of cryogel.
Cross-Linking Type Methods Examples References
Physical cross-linking Ionic interaction Alginate cryogel cross-linked with Ca2+ [12]
Ionic cryotropic gelation of pectin-chitosan [13]
Freezing-thawing cycle PVA-gelatin cryogel; hydrogen bonding, formation of crystallites of PVA [14]
Agarose cryogel, intramolecular and intermolecular hydrogen bonding [15]
Chitosan cryogel, formation of crystallites composed of D-glucosamine and N-acetylglucosamine units [16][17]
Chemical cross-linking Reaction between the functional groups of polymer and cross-linker or polymer–polymer Condensation of an amine with the carbonyl group, formation of Schiff
bases; chitosan and gelatin cross-linked with glutaraldehyde.
HEMA-Gelatin: radical polymerization and cross-linking with
poly(ethylene glycol) diacrylate (PEGDA)
Reactions of multifunctional-amine and epoxy groups; chitosan and
diglycidyl ethers of ethylene glycol (EGDGE) or polyethylene glycol (PEGDGE)
Reaction between amine and carboxy groups: gelatin, gelatin-heparin
cross-linked with EDC/NHS
Oxime-cross-linking of gelatin-oxyamine and hyaluronan-aldehyde;
gelatin-hyaluronan cryogel
Radiation cross-
linking, exposure to high energy source (electron beam)
Electron-beam initiated cross-linking reaction; dextran, hyaluronan [28][29][30]
Photo-cross-linking UV photo-cross-linking; hyaluronic acid, dextran [31][32]
An example of non-covalent ionic-type cross-linking is the preparation of ionic cryogels as a result of the growth of metal-polymer-coordinated complexes and electrostatic interactions between oppositely charged groups of chitosan and metal ions such as PdCl42−, PtBr4 2− and PtCl42− at sub-zero temperatures [33][34]. Alginate-based cryogel can be obtained by introducing ionic links between anionic polysaccharide macromers with divalent cations such as Ca2+ [12].
Self-assembly of peptides is another example of the formation of non-covalent cross-linked cryogels. Peptide cryogels were obtained by pH-sensitive self-assembly of fluorenyl-9-methoxycarbonyl (Fmoc)-diphenylalanine (Phe-Phe) into fibers in an apparently frozen system [35]. Fmoc-Phe-Phe-derived cryogels were noticeably less mechanically strong than equivalent hydrogels prepared from the same concentration of Fmoc-Phe-Phe at ambient temperature. This could be due to the effect of cryostructuration: pre-concentration of Fmoc-Phe-Phe at low temperatures leading to much higher concentrations of Fmoc-Phe-Phe compared to the bulk concentration in the hydrogel precursor solution, which may affect the formation of the fibers. In addition, the formation of large pores creates a more heterogeneous structure in cryogels than in hydrogels, which can reduce the mechanical stability of the material [35]. Usually, cryogels obtained by radical polymerization or covalent cross-linking are mechanically stronger than hydrogels obtained from the same gel precursor solution at room temperature. In the case of Fmoc-Phe-Phe-derived cryogels, the 3D structure was supported by weaker non-covalent interactions, which may explain why cryogels were mechanically less strong.
Covalent cross-linkers are selected based on the chemistry of the polymer used. Bifunctional acrylate derivatives, such as N,N′-methylenebisacrylamide (MBA), poly(ethylene glycol) diacrylate (PEGDA) and others have been used to provide additional connections between formed polymer chains during radical polymerization. A range of different cross-linkers are used for cross-linking biological polymers. 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), alone [36][37] or in combination with N-hydroxysuccinimide (NHS) [38][39], is an important water-soluble activator for carboxyl and phosphate groups of biopolymers that makes them reactive, respectively, toward amino and hydroxyl groups. EDC/NHS generates peptide-like bonds and is well used as a potentially biocompatible cross-linker in the biomaterial fields. EDC is limited to cross-link molecules that are directly adjacent to each other, whereas glutaraldehyde (GA) provides more flexible cross-linking with longer linkage between molecules.
GA has been widely used as a cross-linking agent for collagen, gelatin, chitosan and other polymers due to its low cost, high solubility and stability in aqueous solutions [40][41]. However, the excessive incorporation of GA into scaffolds can have implications for biocompatibility. Some adverse effects of GA modifier, such as the inhibition of cell proliferation, have been reported, and it was suggested that the lowest concentration that does not compromise the mechanical properties of the cryogel should be used [42]. Additionally, to improve biocompatibility of the scaffolds cross-linked with GA, residual aldehyde groups and formed Schiff’s base in the cryogel have to be reduced by sodium borohydride [18][43]. The removal of toxic sodium borohydride itself requires extensive washing of the material, which can be time-consuming and expensive. Alternatively, a hydrophilic polysaccharide dextran pre-modified with aldehyde groups was used as a cross-linker for gelatin and chitosan [44][45]. The viability of mouse embryo NIH 3T3 fibroblasts was considerably higher (∼80%) on modified dextran cross-linked scaffolds than on scaffold cross-linked with GA (40%) [44], which indicates a lower toxicity of the modified dextran cross-linked scaffolds.
Genipin, a natural product extracted from the gardenia fruit, has been utilized as a biocompatible cross-linker to obtain hyaluronic acid (HA) based cryogels [46]. The effect of GA, genipin, and EDC/NHS on the formation of carrageenan-alginate scaffolds by freeze-drying was analyzed [38]. Scaffolds produced using EDC/NHS cross-linking resulted in better cellular response and higher metabolic activity, as well as demonstrating biocompatibility properties, and being porous and physically and mechanically stable. Thus, even where genipin has been proposed as a non-toxic cross-linker, the final choice of cross-linker must be determined by the application and properties of the scaffold, and an alternative cross-linkers must be taken into consideration in order to achieve best results. Another limitation of genipin is that cross-linking produces a material that has a strong blue color, which is not always desirable.
Kirsebom et al. studied the cryotropic gelation of gelatin and casein catalyzed by transglutaminase (TGase) enzyme. TGase catalyzes the linkage of the γ-carboxyamide group of glutamine and the ε-amino group of lysine [47]. The enzymatic cross-linking under partly frozen conditions was a slow process, requiring at least 14 days to form gels that were sufficiently stable to handle. The gels, however, were not formed at room temperature, indicating the role of freezing in the gelation process. The mechanical properties of gelatin and casein cryogels improved with increasing protein content [47]. There is some potential advantage to enzymatic cross-linking, such as mild reaction conditions and no need to use other chemicals as cross-linking agent. However, the enzyme activity under cryogenic conditions is low and cryogel formation takes a long time.
The use of external energy sources, such as UV radiation, is another alternative in the synthesis of cryogels which does not use chemical initiators. Cryogels from mixed semi-dilute solutions of 2-hydroxyethylcellulose and chitosan have been obtained using UV radiation with H2O2 as a photoinitiator [48]. The advantages of this process are the relatively short time required for cryogel formation and the fact that no additional purification is needed. Unfortunately, this method is limited by the low penetration depth of photons. Another approach to cross-linking was used in [28][29][30], where polymerization of acrylated dextran and hyaluronan was initiated by accelerated electrons (an E-beam). This method uses no toxic initiators, and results in sterile, elastic scaffolds with a controlled pore size, excellent swelling and low flow-resistance properties [30]. The reaction time was short—less than 10 min—and with double-sided irradiation, cryogels up to 7 cm thick could be obtained. Some degradation occurred under irradiation along with cross-linking, and the degradation products were washed out, resulting in 80% of the hyaluronan being incorporated into the scaffold [28]. Even though this is a simple cross-linking process, it requires special electron beam equipment and the modification of polysaccharides with acrylate groups, followed by extensive purification before polymerization. Another disadvantage is the limitation of the cryogel size that can be produced and the impossibility of using it to obtain larger samples.

3. Effect of Composition

The composition—that is, the nature of the polymers used, their molecular weight and concentration—have an effect on the final cryogel morphology, mechanical properties and degradation. The porosity and mechanical properties of a HA cryogel cross-linked with ethylene glycol diglycidyl ether (EGDE) has been shown to depend on the HA and cross-linker concentration [49]. Increasing HA concentration decreased cryogel pore size, reduced swelling properties, and reinforced mechanical properties. On the other hand, decreasing cross-linker concentration, at a constant HA concentration, increased the pore size and swelling capacity but provided less rigidity [49]. The composition of gelatin/HA cryogels had an effect on the compressive modulus and other important mechanical characteristics of the cryogels [50]. Larger amounts of gelatin in the composite cryogel increased its Young’s modulus (or stiffness) and toughness due to the formation of additional EDC-mediated cross-links between gelatin and HA molecules, which contributed to the resistance of the struts to bending under compression. A more cross-linked material also showed diminished ultimate strain. However, increasing the gelatin concentration beyond 10% was found to offer an excessive cross-linking rate at the cross-linking temperature (−20 °C) and cross-linker concentration used. This resulted in completion of the gelation process in the polymer solution before porogens (ice crystals) were formed. The cryogels formed under these conditions were very brittle and had low porosity and inferior mechanical strength [50]. These examples show that the composition of the cryogel must be optimized for each specific application with respect to the nature and concentration of the polymer, as well as the cross-linking mechanism.

4. Effect of Freezing Conditions

The freezing temperature affects the pore size and wall thickness of cryogels [19][49]. The highest freezing temperature and the slowest cooling rate produces the biggest pores in cryogels and lower temperature and faster freezing rates are required to produce the smallest pores. The reason for this is the different rates of growth of solvent crystals. Bigger solvent crystals form at slower cooling rates, and this creates bigger pores. At lower temperatures, more solvent will freeze, creating less space between the solvent crystals, where the gel precursors will concentrate forming denser polymer walls. At lower temperatures, the rate of nucleation is higher, which leads to the formation of more crystals of smaller size, thus creating smaller pores in the final cryogel material [19].
In general, the solvent crystals must form before gelation has finished. When cryogels are synthesized by radical polymerization, a lower concentration of initiator is usually used to slow down the polymerization rate and produce cryogels with large pores [51]. If the gelation occurs faster than the ice crystal formation, large, interconnected pores will not form and the cryogel will have a morphology similar to a hydrogel obtained at room temperature. A gel formed under these conditions will have less porosity, less mechanical strength, and will be brittle in nature [50]. Hixon et al. compared cryogel scaffolds with the conventional hydrogel-based scaffolds of three different materials (chitosan-gelatin, N-vinyl-2-pyrrolidone, and silk fibroin (SF)) to identify the optimal material and form of scaffold for use as a graft substitute in bone regeneration [52]. Cryogels proved superior to hydrogels in terms of swelling potential, porosity and mechanical properties, regardless of the polymer used [52]. The microstructure of the cryogels depended on the kinetics of the gelation and nucleation and could be controlled, as mentioned above, by temperature as well as by the concentrations of the initiator or cross-linker [51].
A heterogeneous structure of the cryogels forms under unidirectional freezing. This was observed in the preparation of sheets of cryogels, where the pore size varied from top to bottom. The cryogel had smaller pores at the bottom (in contact with the freezing plate) and the pore size gradually increased towards the top (Figure 2A) [20]. This anisotropic morphology can be beneficial for wound healing because it creates a special environment for cells and controls their migration. When material is applied with smaller pores at the top and larger pores at the wound bed, it stimulates cell migration from the wound into the material. An additional benefit of placing larger pores of the material on the wound bed is increased flexibility to adjust to the roughness and curvature of the wound surface [20].
Figure 2. Anisotropic structure in cryogels: (A) gelatin cryogel frozen in a petri dish, (B) scheme of set up for unidirectional freezing, and (C) the scheme of ice-crystals grows and scanning electron microscopy image of PEG-based cryogel. (Figure (A) is adapted from [2] and used under a Creative Commons Attribution 4.0 International License and (C) is adapted from [53] with permission from The Royal Society of Chemistry.
Many natural tissues, such as those in nerves, cartilage, ligaments, tendons, and the spinal cord, have oriented structures. The morphology of tissue scaffolds should mimic these natural tissues, and cryogels with an aligned porous structure are preferable for better physiological and mechanical function of the tissue scaffold. Unidirectional freezing has been used to create aligned porous structures in both synthetic and natural polymer cryogels [53][54]. During unidirectional freezing, ice crystals form and grow in one direction, forming an aligned porous structure after melting (Figure 2C). Poly(ethylene glycol) cryogels were prepared using PEGDA of various molecular weights: 200, 700 and 2000 Da as a precursor [53]. Cryogels with a smaller pore diameter were obtained using lower molecular weight 200 Da, PEGDA-200, while larger pores were observed when PEGDA with a molecular weight of 2000 Da was used (PEGDA-2000). The smaller pores of the PEGDA-200 cryogel were observed because PEGDA-200 exhibited a marked tendency to adsorb on the surface of the ice crystal during freezing, inhibiting crystal growth. Smaller ice crystals resulted in smaller pore diameters [53]. Freezing temperature was an important parameter for unidirectional freezing because it influenced the rate of formation, size and orientation of solvent crystals. Using a higher temperature leads to the formation of cryogels with large pore diameters. In the work by Wu et al., frozen acetic ether, frozen ethyl alcohol and liquid nitrogen were used to achieve freezing temperatures of −80 °C, −110 °C and −196 °C, respectively. The resulting cryogels had anisotropic compressive strength in accordance with the directions of the pores, and the diameter of the microtubule channels could be controlled in the range 10 to 50 μm by adjusting the molecular weight of the PEGDA and the freezing temperature [53]. Using the same approach, a chitosan-gelatin cryogel with aligned porous structure was prepared inside a polyurethane tube for peripheral nerve regeneration [54].

5. Bacterial Cell Based Cryogels

Cryogels can be made from bacterial cells by cross-linking them with a biocompatible cross-linker [55][56][57]. This approach uses living bacterial cells as the main building blocks for cryogels. Bacterial cryogels were prepared in a one-step process with a low percentage of polymer cross-linker, which formed a three-dimensional porous structure with a well-developed system of interconnected pores [56] (Figure 3). A range of the cryogels were made from whole cells of Escherichia coli [55]Pseudomonas mendocinaRhodoccocus koreensis [56] and Acinetobacter radioresistens [57] and their combination [56]. Various macromolecular cross-linking agents were used, such as oxidized dextran, PVA and polyethyleneimine (PEI), the latter two being activated by GA. The composition of cross-linker agents was optimized so that a high percentage of cells remained viable (50 to 80%) and retained their metabolic activity [56][57]. As can be seen from the SEM image, the walls of the cryogels were mainly composed of whole cells with a very low percentage of polymer, around 1 to 2% (Figure 3C). Bioreactors, based on bacterial cryogels, were effective for the degradation of phenolic compounds [56][57]. The authors believe that the aforementioned approaches can also be used to design cryogels containing some other bacteria with beneficial medical effects or potentially extended to human cells, which nevertheless will require sophisticated conditions to maintain cell viability and activity under cryogenic conditions.
Figure 3. (A) Scheme of bacterial cryogel preparation, (B) bacterial cryogels prepared in syringes, and (C) SEM images of bacterial cryogel and scheme of phenol compound degradation. Figures are adapted from [56] with permission from the Royal Society of Chemistry.

6. 3D Printing of Cryogels

Another step towards creating a complex tissue design with better control over porosity and material heterogeneity is 3D printing. The cryogel 3D printing process has been designed to provide better control over porosity in cryogel samples [58][59]. Carboxymethylcellulose was used as a structural material with cross-linking chemistry of adipic dihydrazide. A multilayer 3D printing of cryogels was performed by controlling the temperature of the dispensed solution, which allows to change the local pore size on demand (Figure 4) [58]. It was possible to control the cell seeding density in vitro, as well as tissue infiltration and vascularization In Vivo by precisely controlling the local geometry of the scaffold in a larger 3D structure [58]. Another example of 3D printing involved the preparation of self-healing, oxime cryogels by cross-linking a poly(n-hydroxyethyl acrylamide-co-methyl vinyl ketone) with a bi-functional hydroxylamine [60].
Figure 4. Principle of 3D cryogel printing. (A) Illustration of 3D printing of cryogels. A viscous precursor solution was printed through a modified commercial printer onto a freezing cold stage. The liquid froze upon stage contact, leading to local self-organization of ice crystals and polymer. After curing at –20 °C and thawing, a cryogel was obtained in the desired shape (in this example, a torus). (B) Illustration of the 4D shape change. By withdrawing fluid, the cryogel was highly dehydrated and compressed; in addition, surface tension drove surface-minimizing folding. Upon rehydration, the cryogel returned to its original shape and volume via shape memory. The switch between compressed and deployed cryogel state was reversible, could be repeated many times, and was used for minimally invasive delivery. (C) Illustration of the hierarchical scaffold organization. Local pore size variation was used to preferentially drive in vitro cellular organization and In Vivo vascularization to areas with large pores. Reprinted from Acta Biomaterialia, Vol 76, Beduer, A.; Piacentini, N.; Aeberli, L.; Da Silva, A.; Verheyen, C.A.; Bonini, F.; Rochat, A.; Filippova, A.; Serex, L.; Renaud, P.; et al. Additive manufacturing of hierarchical injectable scaffolds for tissue engineering. Copyright 2018 [58] with permission from Elsevier.


  1. Lozinsky, V.I. Cryostructuring of Polymeric Systems. 55. Retrospective View on the More than 40 Years of Studies Performed in the A.N. Nesmeyanov Institute of Organoelement Compounds with Respect of the Cryostructuring Processes in Polymeric Systems. Gels 2020, 6, 29.
  2. Savina, I.N.; Ingavle, G.C.; Cundy, A.B.; Mikhalovsky, S.V. A simple method for the production of large volume 3D macroporous hydrogels for advanced biotechnological, medical and environmental applications. Sci. Rep. 2016, 6, 21154.
  3. Gun’ko, V.M.; Savina, I.N.; Mikhalovsky, S.V. Cryogels: Morphological, structural and adsorption characterisation. Adv. Colloid. Interface Sci. 2013, 187, 1–46.
  4. Dogu, S.; Okay, O. Tough organogels based on polyisobutylene with aligned porous structures. Polymer 2008, 49, 4626–4634.
  5. Tureyen, O.E.; Yilmaz, A.; Yakan, S.D.; Yetiskin, B.; Okay, O.; Okay, O.S. Performance of butyl rubber-based macroporous sorbents as passive samplers. Environ. Sci. Pollut. Res. 2021, 28, 3766–3773.
  6. Lozinsky, V.I. Cryogels on the basis of natural and synthetic polymers: Preparation, properties and application. Uspekhi Khimii 2002, 71, 559–585.
  7. Kurochkin, I.I.; Kurochkin, I.N.; Kolosova, O.Y.; Lozinsky, V.I. Cryostructuring of Polymeric Systems: Application of Deep Neural Networks for the Classification of Structural Features Peculiar to Macroporous Poly(vinyl alcohol) Cryogels Prepared without and with the Additives of Chaotropes or Kosmotropes. Molecules 2020, 25, 4480.
  8. Ivanov, A.E.; Ljunggren, L. Thin poly(vinyl alcohol) cryogels: Reactive groups, macropores and translucency in microtiter plate assays. Heliyon 2019, 5, e02913.
  9. Qi, X.; Hu, X.; Wei, W.; Yu, H.; Li, J.; Zhang, J.; Dong, W. Investigation of Salecan/poly(vinyl alcohol) hydrogels prepared by freeze/thaw method. Carbohydr. Polym. 2015, 118, 60–69.
  10. Kumar, A.; Han, S.S. PVA-based hydrogels for tissue engineering: A review. Int. J. Polym. Mater. Polym. Biomater. 2017, 66, 159–182.
  11. Swieszkowski, W.; Ku, D.N.; Bersee, H.E.N.; Kurzydlowski, K.J. An elastic material for cartilage replacement in an arthritic shoulder joint. Biomaterials 2006, 27, 1534–1541.
  12. Ozbilenler, C.; Altundag, E.M.; Gazi, M. Synthesis of quercetin-encapsulated alginate beads with their antioxidant and release kinetic studies. J. Macromol. Sci. Part A Pure Appl. Chem. 2020, 58, 22–31.
  13. Konovalova, M.V.; Markov, P.A.; Popova, G.Y.; Nikitina, I.R.; Shumikhin, K.V.; Kurek, D.V.; Varlamov, V.P.; Popov, S.V. Prevention of postoperative adhesions by biodegradable cryogels from pectin and chitosan polysaccharides. J. Bioact. Compat. Polym. 2017, 32, 487–502.
  14. Ceylan, S.; Gokturk, D.; Bolgen, N. Effect of crosslinking methods on the structure and biocompatibility of polyvinyl alcohol/gelatin cryogels. BioMed. Mater. Eng. 2016, 27, 327–340.
  15. Lozinsky, V.I.; Damshkaln, L.G.; Bloch, K.O.; Vardi, P.; Grinberg, N.V.; Burova, T.V.; Grinberg, V.Y. Cryostructuring of polymer systems. XXIX. Preparation and characterization of supermacroporous (spongy) agarose-based cryogels used as three-dimensional scaffolds for culturing insulin-producing cell aggregates. J. Appl. Polym. Sci. 2008, 108, 3046–3062.
  16. Takei, T.; Nakahara, H.; Ijima, H.; Kawakami, K. Synthesis of a chitosan derivative soluble at neutral pH and gellable by freeze-thawing, and its application in wound care. Acta Biomater. 2012, 8, 686–693.
  17. Takei, T.; Danjo, S.; Sakoguchi, S.; Tanaka, S.; Yoshinaga, T.; Nishimata, H.; Yoshida, M. Autoclavable physically-crosslinked chitosan cryogel as a wound dressing. J. Biosci. Bioengin. 2018, 125, 490–495.
  18. Jurga, M.; Dainiak, M.B.; Sarnowska, A.; Jablonska, A.; Tripathi, A.; Plieva, F.M.; Savina, I.N.; Strojek, L.; Jungvid, H.; Kumar, A. The performance of laminin-containing cryogel scaffolds in neural tissue regeneration. Biomaterials 2011, 32, 3423–3434.
  19. Demir, D.; Boelgen, N. Synthesis and characterization of injectable chitosan cryogel microsphere scaffolds. Int. J. Polym. Mater. Polym. Biomater. 2017, 66, 686–696.
  20. Shevchenko, R.V.; Eeman, M.; Rowshanravan, B.; Allan, L.U.; Savina, I.N.; Illsley, M.; Salmon, M.; James, S.L.; Mikhalovsky, S.V.; James, S.E. The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed In Vivo as a dermal replacement in wound repair. Acta Biomater. 2014, 10, 3156–3166.
  21. Dainiak, M.B.; Allan, I.U.; Savina, I.N.; Cornelio, L.; James, E.S.; James, S.L.; Mikhalovsky, S.V.; Jungvid, H.; Galaev, I.Y. Gelatin-fibrinogen cryogel dermal matrices for wound repair: Preparation, optimisation and in vitro study. Biomaterials 2010, 31, 67–76.
  22. Zoughaib, M.H.; Luong, D.T.; Siraeva, Z.Y.; Yergeshov, A.A.; Salikhova, T.I.; Kuznetsova, S.V.; Kiyamova, R.G.; Abdullin, T.I. Tumor Cell Behavior in Porous Hydrogels: Effect of Application Technique and Doxorubicin Treatment. Bull. Exp. Biol. Med. 2019, 167, 590–598.
  23. Cetin, K.; Alkan, H.; Bereli, N.; Denizli, A. Molecularly imprinted cryogel as a pH-responsive delivery system for doxorubicin. J. Macromol. Sci. A 2017, 54, 502–508.
  24. Bratskaya, S.; Privar, Y.; Nesterov, D.; Modin, E.; Kodess, M.; Slobodyuk, A.; Marinin, D.; Pestov, A. Chitosan Gels and Cryogels Cross-Linked with Diglycidyl Ethers of Ethylene Glycol and Polyethylene Glycol in Acidic Media. Biomacromolecules 2019, 20, 1635–1643.
  25. Kim, I.; Lee, S.S.; Bae, S.; Lee, H.; Hwang, N.S. Heparin Functionalized Injectable Cryogel with Rapid Shape-Recovery Property for Neovascularization. Biomacromolecules 2018, 19, 2257–2269.
  26. Tao, J.; Hu, Y.; Wang, S.J.; Zhang, J.M.; Liu, X.; Gou, Z.Y.; Cheng, H.; Liu, Q.Q.; Zhang, Q.Q.; You, S.L.; et al. A 3D-engineered porous conduit for peripheral nerve repair. Sci. Rep. 2017, 7, 1–13.
  27. Bahlmann, L.C.; Baker, A.E.G.; Xue, C.; Liu, S.; Meier-Merziger, M.; Karakas, D.; Zhu, L.K.; Co, I.; Zhao, S.; Chin, A.; et al. Gelatin-Hyaluronan Click-Crosslinked Cryogels Elucidate Human Macrophage Invasion Behavior. Adv. Funct. Mater. 2021, 18, 2008400.
  28. Reichelt, S.; Naumov, S.; Knolle, W.; Prager, A.; Decker, U.; Becher, J.; Weisser, J.; Schnabelrauch, M. Studies on the formation and characterization of macroporous electron-beam generated hyaluronan cryogels. Radiat. Phys. Chem. 2014, 105, 69–77.
  29. Reichelt, S.; Becher, J.; Weisser, J.; Prager, A.; Decker, U.; Moller, S.; Berg, A.; Schnabelrauch, M. Biocompatible polysaccharide-based cryogels. Mater. Sci. Eng. C Mater. Biol. Appl. 2014, 35, 164–170.
  30. Thones, S.; Kutz, L.M.; Oehmichen, S.; Becher, J.; Heymann, K.; Saalbach, A.; Knolle, W.; Schnabelrauch, M.; Reichelt, S.; Anderegg, U. New E-beam-initiated hyaluronan acrylate cryogels support growth and matrix deposition by dermal fibroblasts. Int. J. Biol. Macromol. 2017, 94, 611–620.
  31. Georgiev, G.L.; Trzebicka, B.; Kostova, B.; Petrov, P.D. Super-macroporous dextran cryogels via UV-induced crosslinking: Synthesis and characterization. Polym. Int. 2017, 66, 1306–1311.
  32. Fan, C.J.; Ling, Y.; Deng, W.S.; Xue, J.Q.; Sun, P.; Wang, D.A. A novel cell encapsulatable cryogel (CECG) with macro-porous structures and high permeability: A three-dimensional cell culture scaffold for enhanced cell adhesion and proliferation. Biomed. Mater. 2019, 14, 055006.
  33. Berillo, D.; Mattiasson, B.; Kirsebom, H. Cryogelation of Chitosan Using Noble-Metal Ions: In Situ Formation of Nanoparticles. Biomacromolecules 2014, 15, 2246–2255.
  34. Berillo, D.; Cundy, A. 3D-macroporous chitosan-based scaffolds with In Situ formed Pd and Pt nanoparticles for nitrophenol reduction. Carbohydr. Polym. 2018, 192, 166–175.
  35. Berillo, D.; Mattiasson, B.; Galaev, I.Y.; Kirsebom, H. Formation of macroporous self-assembled hydrogels through cryogelation of Fmoc-Phe-Phe. J. Colloid Interface Sci. 2012, 368, 226–230.
  36. Chen, C.-H.; Kuo, C.-Y.; Wang, Y.-J.; Chen, J.-P. Dual Function of Glucosamine in Gelatin/Hyaluronic Acid Cryogel to Modulate Scaffold Mechanical Properties and to Maintain Chondrogenic Phenotype for Cartilage Tissue Engineering. Int. J. Mol. Sci. 2016, 17, 1957.
  37. Wang, H.M.; Chou, Y.T.; Wen, Z.H.; Wang, Z.R.; Chen, C.H.; Ho, M.L. Novel Biodegradable Porous Scaffold Applied to Skin Regeneration. PLoS ONE 2013, 8, e56330.
  38. Ki, S.B.; Singh, D.; Kim, S.C.; Son, T.W.; Han, S.S. Effect of cross-linkers in fabrication of carrageenan-alginate matrices for tissue engineering application. Biotechnol. Appl. Biochem. 2013, 60, 589–595.
  39. Sharma, A.; Bhat, S.; Vishnoi, T.; Nayak, V.; Kumar, A. Three-Dimensional Supermacroporous Carrageenan-Gelatin Cryogel Matrix for Tissue Engineering Applications. Biomed. Res. Int. 2013, 2013, 1–15.
  40. Kutlusoy, T.; Oktay, B.; Apohan, N.K.; Silleymanoglu, M.; Kuruca, S.E. Chitosan-co-Hyaluronic acid porous cryogels and their application in tissue engineering. Int. J. Biol. Macromol. 2017, 103, 366–378.
  41. Kathuria, N.; Tripathi, A.; Kar, K.K.; Kumar, A. Synthesis and characterization of elastic and macroporous chitosan-gelatin cryogels for tissue engineering. Acta Biomater. 2009, 5, 406–418.
  42. Gao, S.; Yuan, Z.; Guo, W.; Chen, M.; Liu, S.; Xi, T.; Guo, Q. Comparison of glutaraldehyde and carbodiimides to crosslink tissue engineering scaffolds fabricated by decellularized porcine menisci. Mater. Sci. Eng. C Mater. Biolog. Appl. 2017, 71, 891–900.
  43. Allan, I.U.; Tolhurst, B.A.; Shevchenko, R.V.; Dainiak, M.B.; Illsley, M.; Ivanov, A.; Jungvid, H.; Galaev, I.Y.; James, S.L.; Mikhalovsky, S.V.; et al. An in vitro evaluation of fibrinogen and gelatin containing cryogels as dermal regeneration scaffolds. Biomater. Sci. 2016, 4, 1007–1014.
  44. Akilbekova, D.; Shaimerdenova, M.; Adilov, S.; Berillo, D. Biocompatible scaffolds based on natural polymers for regenerative medicine. Int. J. Biol. Macromol. 2018, 114, 324–333.
  45. Berillo, D.; Volkova, N. Preparation and physicochemical characteristics of cryogel based on gelatin and oxidised dextran. J. Mater. Sci. 2014, 49, 4855–4868.
  46. Johanna, R.; Claude, O.; Norbert, W. Hyaluronic acid cryogels with non-cytotoxic crosslinker genipin. Mater. Lett. X 2019, 4, 100027.
  47. Kirsebom, H.; Elowsson, L.; Berillo, D.; Cozzi, S.; Inci, I.; Piskin, E.; Galaev, I.Y.; Mattiasson, B. Enzyme-Catalyzed Crosslinking in a Partly Frozen State: A New Way to Produce Supermacroporous Protein Structures. Macromol. Biosci. 2013, 13, 67–76.
  48. Stoyneva, V.; Momekova, D.; Kostova, B.; Petrov, P. Stimuli sensitive super-macroporous cryogels based on photo-crosslinked 2-hydroxyethylcellulose and chitosan. Carbohydr. Polym. 2014, 99, 825–830.
  49. Oelschlaeger, C.; Bossler, F.; Willenbacher, N. Synthesis, Structural and Micromechanical Properties of 3D Hyaluronic Acid-Based Cryogel Scaffolds. Biomacromolecules 2016, 17, 580–589.
  50. Chang, K.H.; Liao, H.T.; Chen, J.P. Preparation and characterization of gelatin/hyaluronic acid cryogels for adipose tissue engineering: In vitro and In Vivo studies. Acta Biomater. 2013, 9, 9012–9026.
  51. Hwang, Y.S.; Zhang, C.; Varghese, S. Poly(ethylene glycol) cryogels as potential cell scaffolds: Effect of polymerization conditions on cryogel microstructure and properties. J. Mater. Chem. 2010, 20, 345–351.
  52. Hixon, K.R.; Eberlin, C.T.; Kadakia, P.U.; McBride-Gagyi, S.H.; Jain, E.; Sell, S.A. A comparison of cryogel scaffolds to identify an appropriate structure for promoting bone regeneration. Biomed. Phys. Eng. Express 2016, 2, 035014.
  53. Wu, J.; Zhao, Q.; Sun, J.; Zhou, Q. Preparation of poly(ethylene glycol) aligned porous cryogels using a unidirectional freezing technique. Soft Matter 2012, 8, 3620–3626.
  54. Singh, A.; Shiekh, P.A.; Das, M.; Seppälä, J.; Kumar, A. Aligned Chitosan-Gelatin Cryogel-Filled Polyurethane Nerve Guidance Channel for Neural Tissue Engineering: Fabrication, Characterization, and in vitro Evaluation. Biomacromolecules 2019, 20, 662–673.
  55. Zaushitsyna, O.; Berillo, D.; Kirsebom, H.; Mattiasson, B. Cryostructured and Crosslinked Viable Cells Forming Monoliths Suitable for Bioreactor Applications. Top. Catal. 2014, 57, 339–348.
  56. Al-Jwaid, A.K.; Berillo, D.; Savina, I.N.; Cundy, A.B.; Caplin, J.L. One-step formation of three-dimensional macroporous bacterial sponges as a novel approach for the preparation of bioreactors for bioremediation and green treatment of water. RSC Adv. 2018, 8, 30813–30824.
  57. Berillo, D.A.; Caplin, J.L.; Cundy, A.B.; Savina, I.N. A cryogel-based bioreactor for water treatment applications. Water Res. 2019, 153, 324–334.
  58. Beduer, A.; Piacentini, N.; Aeberli, L.; Da Silva, A.; Verheyen, C.A.; Bonini, F.; Rochat, A.; Filippova, A.; Serex, L.; Renaud, P.; et al. Additive manufacturing of hierarchical injectable scaffolds for tissue engineering. Acta Biomater. 2018, 76, 71–79.
  59. Serex, L.; Braschler, T.; Filippova, A.; Rochat, A.; Beduer, A.; Bertsch, A.; Renaud, P. Pore Size Manipulation in 3D Printed Cryogels Enables Selective Cell Seeding. Adv. Mater. Technol. 2018, 3, 1700340.
  60. Nadgorny, M.; Collins, J.; Xiao, Z.Y.; Scales, P.J.; Connal, L.A. 3D-printing of dynamic self-healing cryogels with tuneable properties. Polym. Chem. 2018, 9, 1684–1692.
Contributor MDPI registered users' name will be linked to their SciProfiles pages. To register with us, please refer to :
View Times: 1019
Revisions: 2 times (View History)
Update Date: 12 Jul 2021