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    Chromatin Accessibility Regulates White-Opaque Switching

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    Submitted by: Clarissa Nobile

    Definition

    Candida albicans, a diploid polymorphic fungus, has evolved a unique heritable epigenetic program that enables reversible phenotypic switching between two cell types, referred to as “white” and “opaque”. These cell types are established and maintained by distinct transcriptional programs that lead to differences in metabolic preferences, mating competencies, cellular morphologies, responses to environmental signals, interactions with the host innate immune system, and expression of approximately 20% of genes in the genome. Transcription factors that regulate the establishment and heritable maintenance of the white and opaque cell types have been a primary focus of investigation in the field; however, other factors that impact chromatin accessibility, such as histone modifying enzymes, chromatin remodelers, and histone chaperone complexes, also modulate the dynamics of the white-opaque switch and have been
    much less studied to date.

    1. Introduction

    Multicellular organisms are comprised of many phenotypically and functionally distinct cell types, the vast majority of which contain the same primary genomic sequence. How a single set of genomic “instructions” can reliably yield many distinct and heritable phenotypic states is a fundamental question in biology. We have begun to understand that a single genome can support many transcriptional programs, which in turn specify unique cell type specific patterns of gene expression, and ultimately establish distinct phenotypes. These cell types are often heritably maintained in an epigenetic manner following each cell division, and it has become increasingly apparent that chromatin structure and accessibility play important roles in the transcriptional regulation of cell type specificity.

    Candida albicans, a unicellular polymorphic fungus, has evolved the ability to establish two transcriptional programs that give rise to two distinct cell types called “white” and “opaque” based on their appearance at the single colony level. The white and opaque cell types are heritably maintained in an epigenetic manner through thousands of cell divisions with no change to the primary sequence of the genome [1][2]. A growing body of literature has identified numerous similarities between the molecular mechanisms governing the C. albicans white-opaque switch and those that underlie heritable cell type differentiation in higher eukaryotes [3][4][5][6][7]. Since a similar heritable phenotypic switch is not observed in the classic model yeast Saccharomyces cerevisiae, C. albicans has emerged as a compelling “simple” and genetically tractable eukaryotic model system to study heritable transcriptional programs in higher eukaryotes.

    The C. albicans white and opaque cell types are established and maintained by distinct transcriptional programs that lead to a wide range of phenotypic differences between the two cell types. These include differences in metabolic preferences, mating competencies, cellular morphologies, responses to environmental signals, interactions with the host innate immune system, and expression of ~20% of genes in the genome [3][4][8][9][10][11][12][13][14][15]. A variety of environmental cues have been identified that can bias the switch in favor of the white or opaque cell type. Growth in the presence of N-acetyl glucosamine, elevated CO2 levels, acidic pH, anaerobic conditions, genotoxic or oxidative stress, and 25 °C all promote white to opaque switching, while 37 °C in the presence of glucose triggers en masse opaque to white switching [1][2][16][17][18][19][20][21]. The destabilizing effect of elevated temperature on opaque cells is not universal, however, and opaque cells can be heritably maintained at 37 °C when grown on alternative (i.e., non-glucose) carbon sources [22]. Under standard switch permissive laboratory growth conditions (25 °C on Lee’s medium supplemented with 100 μg/mL uridine and 2% glucose, or other similarly comprised synthetic defined growth medium), phenotypic switching between the two cell types occurs stochastically at a frequency of approximately one switch event per 1000–10,000 cell divisions [16][17][18][19]. In other words, once established, each cell type is maintained through an epigenetic mechanism that is stably inherited over thousands of subsequent cell divisions.

    2. Regulation of White-Opaque Switching by Chromatin Remodeling Complexes

    Chromatin remodeling enzyme complexes modulate chromatin accessibility through the function of their ATPase-translocase domains. We can classify chromatin remodeling enzymes into four subfamilies, each of which carries out specialized functions [23]. ISWI and CHD complex subfamilies preferentially reduce chromatin accessibility by regulating the assembly and organization of nucleosomes. The SWI/SNF complex subfamily remodels chromatin by sliding or evicting nucleosomes, which generally increases chromatin accessibility [23]. The INO80 complex subfamily modulates chromatin accessibility by replacing canonical histones with histone variants, specifically targeting nucleosomes that flank transcription start sites. The SWR1 complex, a member of the Ino80 subfamily, is the only known regulator of this class that regulates white-opaque switching in C. albicans [5] and is discussed in more detail below.

    Regulation of White-Opaque Switching by the SWR1 Chromatin Remodeling Complex

    SWR1 encodes a chromatin remodeling enzyme that is responsible for the deposition of the histone variant H2AZ. The SWR1 complex, which is an ortholog of the human SRCAP complex, is a multiprotein complex responsible for replacing canonical histone H2A-H2B dimers with the histone variant H2A.Z-H2B dimers without disassembling the H3/H4 tetramer from DNA [24][25]. H2A.Z is a highly conserved variant of H2A that is found throughout all eukaryotes [26]. Developmentally regulated genomic loci show increased enrichment of H2A.Z relative to non-developmentally regulated loci [27]. H2A.Z is deposited specifically into the two nucleosomes that flank transcription start sites [28], and is essential in several higher eukaryotic organisms, but not in fungi [29][30]. In C. albicans, H2A.Z is enriched in white cells, relative to opaque cells, within the upstream intergenic region of WOR1 [5]. The complex responsible for depositing this histone variant appears to play a role in stabilizing the white cell type and destabilizing the opaque cell type, as deletion of SWR1 causes a significant increase in the white to opaque switch frequency and in the heritable maintenance of opaque cells [5]. Since H2A.Z variant enriched sites have been shown to correlate with slightly increased chromatin accessibility relative to canonical histones [31], it is conceivable that higher levels of H2A.Z inhibit expression of WOR1 by facilitating the binding of a repressor protein within the upstream intergenic region of WOR1.

    A similar phenotype is observed upon disruption of the NuA4 complex, which is known to recruit and/or promote chromatin-related enzymatic activities of the SWR1 complex [32][33]. Therefore, it is likely that NuA4 regulates the white-opaque switch by modulating the recruitment or enzymatic activity of Swr1, which in turn results in decreased H2A.Z deposition throughout the genome. The nucleosome editing function of the SWR1 complex is also controlled through H3K56 acetylation, which is catalyzed by Rtt109. High levels of H3K56 acetylation led to decreased levels of H2A.Z deposition genome-wide [211]. This is notable as H3K56 acetylation itself has been implicated in altering histone turnover rates [212], which consequently alters genome-wide chromatin accessibility. It remains an open question whether H3K56 acetylation regulates the white-opaque switch by modulating the enzymatic activity of the SWR1 complex, or whether H3K56 acetylation directly regulates the white-opaque switch by modulating histone turnover rates.

    3. Regulation of White-Opaque Switching by Histone Chaperone Complexes

    The highly basic amino acid composition of histones makes them predisposed to aggregation and promiscuous histone-DNA interactions, thus necessitating a diverse network of histone chaperones to orchestrate the assembly and integration of histones into chromatin [34][35][36]. Below, we focus our discussion on the evolutionarily conserved histone chaperone complexes HIR (HIRA in humans) and CAF-1, and their roles in regulating the white-opaque switch in C. albicans. CAF-1 primarily assembles nucleosomes in a replication dependent manner [37][38], whereas HIR functions independent of replication [39][40]. Importantly, the replication coupled nucleosome assembly function of CAF-1 is conserved in humans [37][38]. Both chaperone complexes are essential in higher eukaryotes [41][42], which has complicated efforts to investigate their functions in cell type formation and maintenance. The C. albicans white-opaque switch provides a unique and robust alternative system to investigate the functions of these highly conserved chaperone complexes in higher eukaryotes.

    Studies in both S. cerevisiae and human HeLa cells have revealed that the HIR and CAF-1 complexes modulate nucleosome dynamics [43], which in turn affect chromatin accessibility. Other than their replication dependent functions, these two enzymes have also been shown to have several overlapping functions that are unrelated to replication. Recent work in C. albicans has shown that they function similarly to their orthologs in S. cerevisiae. Deletion of C. albicans HIR1, a subunit of the HIR complex, had no effect on white-opaque switching, while deletion of CAC2, a subunit of CAF-1 complex, resulted in an overall increase in switching in both directions [44]. On the other hand, deletion of a subunit of both chaperone complexes in C. albicans has been shown to lead to reduced opaque cell stability, as evidenced by wildtype levels of white to opaque switching and a sixfold increase in opaque to white switching [44]. These results alone do not definitively point to a specific chaperone complex responsible for regulating opaque cell stability; however, they do reveal that nucleosome dynamics can significantly affect cell type maintenance in the context of the white-opaque switch. Modulating nucleosome dynamics has a significant effect on chromatin accessibility [45], and recent studies have acknowledged the impact of chromatin accessibility on cell type specification and maintenance [46][47][48][49][50]. It is possible that opaque cells, more so than white cells, depend on increased chromatin accessibility to maintain their cell type specific transcriptional program, which could explain why deleting subunits of the HIR and CAF-1 complexes have dramatic effects on opaque cell stability.

    The entry is from 10.3390/jof7010037

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