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    Topic review

    Model for MCMV Disease

    Subjects: Virology
    View times: 38
    Submitted by: Megan Lloyd

    Definition

    Murine cytomegalovirus (MCMV) is a natural pathogen of mice that is present in all wild mice populations and has been used extensively as an animal model for human cytomegalovirus disease. By manipulating the mouse strain, the type of virus used, the inoculation route and the addition of various chemotherapeutic treatments (e.g. immunosuppression), many human diseases can be modeled in a realistic and consistent way. This entry describes the many ways that MCMV has been used to model human disease.

    1. Introduction

    It has famously been stated that “mice lie and monkeys exaggerate”[1], but the use of animal models in the study of infectious disease provides strong evidence for the mechanisms underlying the pathogenesis of infections in humans and provides opportunities to study interactions between hosts and pathogens that are not provided by collecting minimally invasive human samples such as blood, feces, saliva and urine. When investigating infectious diseases, the ability to correlate tissue histopathology with pathogen replication rates over time or evaluate cell signaling and related immune modulation in the context of a complete immune system is of inestimable value and provides mechanistic information, as well as pre-clinical opportunities to test treatment efficacy and toxicity. The ability of a pathogen to infect both mice and humans allowed the development of Koch’s postulates[2], an evidence framework still used today to prove the association of newly described pathogens with infection and subsequent pathological sequelae, albeit with modifications to account for the presence of pathogens in commensal flora[3] and viruses that are attenuated in a culture[4]. An example of this modern reasoning has been applied for SARS-CoV-1[5].

    There are many animal models where laboratory-inoculated animals exhibit symptoms or pathogen replication that mimics human infection, even though infection may not occur in the natural environment. These models are useful in carefully evaluated (and disclosed) circumstances (discussed in[6] for SARS-CoV-2) and have provided useful information on infection pathways and the effects of opportunistic infections and potential drug targets[7]. Examples of animal models in non-target species are those developed for influenza and respiratory syncytial virus in ferrets[8] and Ebola virus infection, which have been modeled in suckling (but not adult) immunocompetent mice as well as Syrian golden hamsters[9]. These models and others like them have illuminated pathways used by pathogens to travel from the site of infection to organs or other sites of tropism, as well as immune responses, symptom and infection profiles and treatment options.

    In animal models for viral disease, the presence of the correct receptor used by the virus to enter a cell is critical and needs to be largely conserved between species for cross-species infection to occur[6]. For infections where viruses do not naturally infect another species due to absence of the correct receptor, transgenic mice have been developed that allow an animal model to be developed (e.g., for poliovirus)[10]. Some viruses, such as influenza, naturally infect multiple species (avian species, pigs and humans[11]) while others, such as cytomegalovirus, are species-specific. Animal models where the pathogen naturally infects the animal host and the disease is naturally present in wild populations can provide insights into human disease by illuminating complex host-pathogen interactions and provide clinically relevant data.

    Cytomegaloviruses (CMVs) from the family Herpesviridae and the subfamily Betaherpesvirinae are species-specific viruses and have been isolated from many mammalian species (e.g., human, mouse, rat, guinea pig and various primates; see[12] for a discussion). CMVs have evolved with their hosts[13] and generally do not replicate completely in vivo in different species after inoculation, even where in vitro growth has been described (e.g., murine cytomegalovirus (MCMV) will grow in vitro in rat cells but will not grow in vivo in rats[14]). However, there have been reports of the replication of disparate CMV in other species in circumstances where xenotransplantation has taken place and the recipient has received immunosuppressive chemotherapy[15][16]. Juvenile rats have also been reported to support the growth of MCMV, although older rats (>6 weeks old) were not susceptible to infection[17].

    2. MCMV as a Model for HCMV Infection

    The recorded movement of MCMV between organs differs, depending on the route of infection (described in[33] and [12]). There is a strong tropism for the salivary gland, and active viral replication persists in salivary glands for longer than other organs, regardless of the route of infection used. As with all herpesviruses, MCMV infection has a latent phase[32]. MCMV infection without significant manipulation of the host has successfully been used to model various aspects of human infection (Table 1). Information from this natural animal model has greatly improved the understanding of the pathogenesis of HCMV infection in humans.

    Table 1. Human disease caused by human cytomegalovirus (HCMV) modeled by murine cytomegalovirus (MCMV) infection in mice.

    Human Condition Lab Conditions SGV 1/TCV 2
    PFU 3
    Effect Reference
    Viremia Intraperitoneal (i.p) inoculation of BALB/cByJ mice. SGV/TCV not specified
    106 PFU
    White blood cells have viral DNA but no evidence of ie1 RNA. [34]
    Viral latency BALB/c footpad inoculated at 2 weeks of age. Latency present after 3 weeks. TCV
    105 PFU
    Whole body irradiation leads to reactivation of infection. Antibody protects from viral dissemination. [35][36]
    Pneumonitis 1. Intranasal inoculation into outbred Swiss mice or intra-tracheal infection of BALB/c mice. TCV
    >104 PFU
    Severe diffuse interstitial pneumonitis closely resembling that seen in immunocompromised patients and in newborn infants, 20% died. [37]
    2. Inoculation of newborn BALB/c. SGV, 6 PFU i.p. Pneumonitis and myocarditis, 95% lethal. [38]
    Hepatitis i.p inoculation of BALB/c mice. SGV, 105 PFU Hepatitis evident, dose is Lethal Dose50. [26]
    Ocular infection (retinitis) 1. Intraocular inoculation (scarified cornea or through corneal limbus) of IRC/Sic mice.
    2. Intraperitoneal inoculation of BALB/c mice.
    SGV, Tissue Culture Infectious Dose50 values given. Different effects SGV vs. TCV. [39]
    Inflammatory response in retina (virus not present) and iris (virus present). [40]
    Excretion of CMV into breastmilk Acute or latently inoculated C57BL/6 mothers (i.p.), leukocytes from BM positive by ie1 mRNA detection, RTPCR. SGV
    3 × 102–3 × 104 PFU
    Evidence of neonatal infection via breast milk. Inoculation of milk into CD-1 1-day old mice results in infection. [41]
    Arterial blood pressure post CMV infection i.p. inoculation of 2-week-old C57BL/6 mice. TCV,
    3 × 105 PFU/1mL/mouse
    MCMV increased blood pressure independent of diet. Increased serum IL-6, TNF-alpha, and MCP-1. [42]
    Viral myocarditis i.p. inoculation of C57BL/6 and BALB/c mice. SGV
    104 PFU
    Inflammatory foci in the heart and infection of cardiac myocytes. [43]
    Infection post bone marrow transplantation Irradiated BALB/c mice inoculated with virus prior to intravenous (i.v.) purified bone marrow cells. 105 PFU Failure in haematopoiesis, leading to death. [44]
    Sexual transmission of CMV via semen Spermatozoa plus Smith MCMV artificially inseminated (compared with sperm alone).[47] SGV
    105 PFU
    Embryos collected on E14. One produced cytopathic effect (second passage). No significant difference with numbers or abnormalities. [45]
    1 Salivary gland derived virus. 2 Tissue culture-derived virus. 3 Plaque-forming units.

    3. Mouse Strain Selection Affects the Severity of MCMV Pathogenesis

    Investigations of the effect of MCMV in different mouse strains have been pivotal in the modeling of different disease states. Initial work focused on LD50 calculations (e.g.,[46]), and it was found that the H-2 alleles of different mouse strains determined their response to infection, including the production of autoantibodies[47] and the induction of myocarditis[48]. Other mechanisms for resistance to MCMV, such as that demonstrated in C57BL/6 mice compared with BALB/c mice or the differing resistance of New Zealand Black and White mice to MCMV infection, were associated with differences in innate natural killer (NK) cell activation and were strongly associated with particular strains of viruses[49][50]. Where MCMV was used as a vaccine vector expressing the mouse ovarian glycoprotein zona pellucida 3 in studies investigating immune-mediated contraception, the specific m157 (viral ligand) to Ly49H (NK cell activation receptor) rapid response to infection was broadly associated with vaccine success[51]. This effect was abrogated through the use of a different virus strain, G4 (isolated from the salivary glands of a mouse from Geraldton, WA[52]), as the vaccine vector. G4 does not have the same interaction with NK cell activation receptors, and this demonstrates the importance of vector strain selection in the development of recombinant CMV-based vaccines[53].

    4. MCMV-Based Models for Human Disease Requiring Chemical, Genetic or Physical Manipulation

    A recent systematic review and metanalysis calculated that the worldwide seroprevalence of HCMV is 83%[54]. Increasing CMV disease is broadly associated with improvements in medicine because it is often associated with acute immunosuppression, allowing the reactivation of a latent infection. One of the more serious sequelae of CMV infection is found in solid organ transplant recipients, who often experience reactivation from latency and associated pneumonitis, hepatitis and potential organ rejection (discussed in[55]). The likelihood of severe CMV disease increases when the transplanted organ is from a seropositive donor (previously infected with CMV, with no active viral replication but a strong CMV-specific humoral response) being transplanted into a seronegative recipient[56]. In general, outcomes can be improved with the use of antiviral therapy, with a recent metanalysis suggesting that prophylactic treatment using low doses of valganciclovir provides improved outcomes in kidney transplant recipients [57]. CMV can also cause post-transplantation disease in recipients of allogeneic hematopoietic stem cell transplants, and pre-emptive therapy is often initiated after clinical evidence of CMV reactivation (prior to fulminant disease). The economic burden of this therapy is marked[58]. The mouse model of MCMV has been integral in the prediction of useful therapeutics for these clinical circumstances (reviewed in[59], with a discussion of the appropriate use for this model in reliably predicting human outcomes).

    In order to model CMV-associated diseases occurring due to immunosuppression, such as retinitis or post-transplantation reactivation, the animal model needs to be manipulated to ensure similarity to human infection. The modification can be due to chemical administration (e.g., corticosteroid use), genetic modification of either the virus or the mouse strain used (e.g., MCMV-deleted m157 virus (Δ157) allows C57BL/6 mice to be used without NK cell activation by the virus) or physiological treatment such as surgery. These modified models are listed in Table 2.

    Table 2. Models for CMV disease, requiring significant laboratory manipulation.

    5. MCMV Exacerbates the Effects of Other Clinical Diseases

    For some models, the addition of MCMV can exacerbate disease, reflecting human disease particularly in the intensive care unit setting. These are generally diseases with an immune modulation component. These models are listed in Table 3.

    Table 3. Models demonstrating MCMV-associated effects on other diseases.

    This entry is adapted from 10.3390/ijms22010214

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