Background: Soft tissue regeneration and remodeling is fundamental in periodontal surgery, thus we investigated the angiogenic response elicited in the subcutaneous tissue of rats by a proprietary, polyethylene glycol hydrogel formulation (PEG) alone or conjugated with specific amelogenins (EMD) or nanobioglass particles (NBG).
Methods: Discs with three different formulations (PEG, PEG-EMD, and PEG-NBG) were inserted into four unconnected subcutaneous pouches, produced on the back of Sprague-Dawley rats (n=56, divided into three groups), and used for blood flow evaluation by Laser Doppler analysis at 1, 2, 4, 8, and 16 weeks or for histological and immunohistochemical analysis at 1, 2, 4, 8, and 16 weeks.
Results: All formulations showed tissue integration, absence of inflammatory reaction (as revealed by myeloperoxidase staining), and increased vascularization (by counting microvascular density following CD31 staining). Laser Doppler analysis revealed a statistically significant increase in blood flow after 1 week for PEG-EMD and after 2 weeks for PEG-NBG. The angiogenic response was significantly increased at 1, 2, and 8 weeks for PEG-EMD, but only at 4 weeks for PEG-NBG.
Conclusions: The studied biomaterials revealed equal biocompatibility and tissue integration properties. PEG-EMD showed the most pronounced and consistent angiogenic response in the early phases of wound healing, while the PEG-NBG formulation provided a slower and delayed, but relevant, response.
- polyethylene glycol;angiogenesis;hydrogel;Formulations Show;periodontal;Subcutaneous;angiogenic;Glycol Formulations;Different Soft;EMD
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- Herten M., Jung R.E., Ferrari D., et al. Biodegradation of different synthetic hydrogels made of polyethylene glycol hydrogel/RGD-peptide modifications: an immunohistochemical study in rats. Clin Oral Implants Res 20,116, 2009
- Thoma D.S., Dard M.M., Hälg G.A., Ramel C.F., Hämmerle C.H., and Jung R.E. Evaluation of a biodegradable synthetic hydrogel used as a guided bone regeneration membrane: an experimental study in dogs. Clin Oral Implants Res 23,160, 2012
- Jung R.E., Zwahlen R., Weber F.E., Molenberg A., van Lenthe G.H., and Hammerle C.H. Evaluation of an in situ formed synthetic hydrogel as a biodegradable membrane for guided bone regeneration. Clin Oral Implants Res 17,426, 2006
- Jung R.E., Hämmerle C.H., Kokovic V., and Weber F.E. Bone regeneration using a synthetic matrix containing a parathyroid hormone peptide combined with a grafting material. Int J Oral Maxillofac Implants 22,258, 2007
- Sculean A., Schwarz F., Becker J., and Brecx M. The application of an enamel matrix protein derivative (Emdogain) in regenerative periodontal therapy: a review. Med Princ Pract 16,167, 2007
- Kauvar A.S., Thoma D.S., Carnes D.L., and Cochran D.L. In vivo angiogenic activity of enamel matrix derivative. J Periodontol 81,1196, 2010
- Hu X., Zhang P., Xu Z., Chen H., and Xie X. GPNMB enhances bone regeneration by promoting angiogenesis and osteogenesis: potential role for tissue engineering bone. J Cell Biochem 114,2729, 2013
- Waltimo T., Brunner T.J., Vollenweider M., Stark W.J., and Zehnder M. Antimicrobial effect of nanometric bioactive glass 45S5. J Dent Res 86,754, 2007
- Kilkenny C., Browne W.J., Cuthill I.C., Emerson M., and Altman D.G. Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research. PLoS Biol 8,e1000412, 2010 [PMC free article]
- Bunyaratavej P., and Wang H.L. Collagen membranes: a review. J Periodontol 72,215, 2001
- Bitto A., Polito F., Altavilla D., Minutoli L., Migliorato A., and Squadrito F. Polydeoxyribonucleotide (PDRN) restores blood flow in an experimental model of peripheral artery occlusive disease. J Vasc Surg 48,1292, 2008
- Galeano M., Bitto A., Altavilla D., et al. Polydeoxyribonucleotide stimulates angiogenesis and wound healing in the genetically diabetic mouse. Wound Repair Regen 16,208, 2008
- Polito F., Bitto A., Galeano M., et al. Polydeoxyribonucleotide restores blood flow in an experimental model of ischemic skin flaps. J Vasc Surg 55,479, 2012
- Shirakata Y., Eliezer M., Nemcovsky C.E., et al. Periodontal healing after application of enamel matrix derivative in surgical supra/infrabony periodontal defects in rats with streptozotocin-induced diabetes. J Periodontal Res 49,93, 2014