N2O Formation in Rice Plants
Rice plants in paddy emit a substantial amount of nitrous oxide (N2O), a potent greenhouse gas to the atmosphere. Field-based studies that report N2O fluxes from the paddy consider that the N2O emitted by rice plant is of microbial origin in the soil. Recent studies on other plants suggest that vegetation are also a natural source of N2O. However, the mechanisms of N2O formation in plants are unknown; consequently, the rice plant is regarded as a channel to transport soil micro-organisms produced N2O. The hypothesis that rice plants are a medium to transport soil produced N2O is based on flux measurement methods. However, more robust methods like 15N isotope analysis methods consider plants are a natural source of N2O. This led us to search for the possible pathway of N2O formation in rice plants cells. Herein, we have proposed a potential pathway of N2O formation in rice plants.
Paddies are a potential site of N2O emissions to the atmosphere. In paddies, not only the soil but also the rice plants are reported to emit a substantial amount of N2O and the rice plant is regarded as a medium to channel the soil produced N2O. However, the hypothesis that rice plants are a medium to transport soil produced N2O is based on flux measurement method. Recent studies based on more robust methods like 15N isotope analysis and aseptically grown plants suggest that plants cells may also produce the N2O. So, the substantial amount of N2O emitted by rice plants might have been formed in the rice plants cells. So, there is a need to explore the possible pathway of N2O formation in rice plants cells.
15N isotope labelling studies have shown that nitrate (NO3-) is a precursor of N2O formation in plants. Similar to NO3-, the addition of 15N labelled nitrite (NO2-) lead to N2O formation in plants. In addition, eukaryotic cytochrome c oxidase when supplied with 15N labelled NO produced N2O. In this scenario, we predicted NO3-NO2-NO pathway might contribute to N2O formation in rice plant’s cells.
2. Potential Pathway of N2O Formation in Rice Plants
NO is a signalling molecule at the cellular level and is formed at every eukaryotic cell. There are several pathways and sites of NO formation in the cell. Among the several pathways of NO formation, a reductive pathway is NO3 and NO2 dependent and occurs during oxygen-limited condition in cytosol and mitochondria, respectively. NO3 is further converted to NO2 in the cytosol with the help of nitrate reductase (NR) and the NO2 can enter the mitochondria with the help of unknown transporter. Then the NO2 can be further reduced to NO by various electron transport chains and the conversion is more favourable in oxygen limitation condition. The NO formed at mitochondria can be further reduced to N2O by reduced form of eukaryotic cytochrome c oxidase and the conversion is more favourable when the oxygen level in the cell is low. As NO2 dependent pathway of NO formation in mitochondria is active during oxygen-limited condition and NO-dependent N2O formation is also favourable during oxygen-limited condition in mitochondria, suggests the N2O emitted by rice plants might have formed at mitochondria when the cell experience hypoxia and anoxia.
At the cellular level there exists the pathway of N2O formation through NO3-NO2-NO pathway as reported in various studies. So, considering rice plants only as a medium to transport the soil-microorganism produced N2O will be misleading. To mitigate the effects of global warming and ozone depletion effectively, a good understanding of all of the sources of N2O and the regulating factors is crucial. So understanding the role of rice plants (i.e., source or medium to channel or both) in paddies is crucial. Further research at the cellular level would insight to the proposed pathway of N2O formation in the rice plants. Furthermore, field-based studies should evaluate the N2O fluxes from the rice plants only (excluding soil) that could insight the rice plants role to the total emissions of N2O from paddies. Moreover, as various other plants species are also reported to emit a substantial amount of N2O, there is a current need to search for the possible pathways of N2O formation in plants cells.
The entry is from 10.3390/plants9020180
- Bruce Linquist; Kees Jan Van Groenigen; Maria Arlene Adviento‐Borbe; Cameron Pittelkow; Chris Van Kessel; An agronomic assessment of greenhouse gas emissions from major cereal crops. Global Change Biology 2011, 18, 194-209, 10.1111/j.1365-2486.2011.02502.x.
- K. W. Yu; Z. P. Wang; G. X. Chen; Nitrous oxide and methane transport through rice plants. Biology and Fertility of Soils 1997, 24, 341-343, 10.1007/s003740050254.
- X Yan; Pathways of N2O emission from rice paddy soil. Soil Biology and Biochemistry 2000, 32, 437-440, 10.1016/s0038-0717(99)00175-3.
- Katharina Lenhart; Thomas Behrendt; Steffen Greiner; Jörg Steinkamp; Reinhard Well; Anette Giesemann; F. Keppler; Nitrous oxide effluxes from plants as a potentially important source to the atmosphere. New Phytologist 2018, 221, 1398-1408, 10.1111/nph.15455.
- Naoki Goshima; Toshihiro Mukai; Mamiko Suemori; Misa Takahashi; Michel Caboche; Hiromichi Morikawa; Emission of nitrous oxide (N2O) from transgenic tobacco expressing antisense NiR mRNA. The Plant Journal 1999, 19, 75-80, 10.1046/j.1365-313x.1999.00494.x.
- Makoto Hakata; M. Takahashi; W. Zumft; Atsushi Sakamoto; H. Morikawa; Conversion of the Nitrate Nitrogen and Nitrogen Dioxide to Nitrous Oxides in Plants. Acta Biotechnologica 2003, 23, 249-257, 10.1002/abio.200390032.
- G W Brudvig; T H Stevens; S I Chan; Reactions of nitric oxide with cytochrome c oxidase.. Biochemistry 1980, 19, 5275–5285, 10.1021/bi00564a020.
- Rőszer, T. The Biology of Subcellular Nitric Oxide; Springer: Dordrecht: The Netherlands, 2012; pp. 3.
- Kapuganti J. Gupta; Alisdair R. Fernie; Werner M. Kaiser; Joost Van Dongen; On the origins of nitric oxide. Trends in Plant Science 2011, 16, 160-168, 10.1016/j.tplants.2010.11.007.
- Alejandro Chamizo-Ampudia; Emanuel Sanz-Luque; Angel Llamas; Aurora Galvan; Emilio Fernandez; Eduardo Perez-Albela Fernandez; Nitrate Reductase Regulates Plant Nitric Oxide Homeostasis. Trends in Plant Science 2017, 22, 163-174, 10.1016/j.tplants.2016.12.001.
- Kapuganti J. Gupta; Abir U. Igamberdiev; The anoxic plant mitochondrion as a nitrite: NO reductase. Mitochondrion 2011, 11, 537-543, 10.1016/j.mito.2011.03.005.
- X.J. Zhao; V. Sampath; W.S. Caughey; Cytochrome c Oxidase Catalysis of the Reduction of Nitric Oxide to Nitrous Oxide. Biochemical and Biophysical Research Communications 1995, 212, 1054-1060, 10.1006/bbrc.1995.2076.