- Please check and comment entries here.
βTCP-Poly(3hydroxybutyrate) for Bone Tissue Engineering
Tissue engineering is a technique that involves the in vitro seeding and attachment of cells onto a three-dimensional scaffold. In the case of bone tissue engineering, investigations have been focused mostly on synthetic bioceramic scaffolds based on calcium phosphates, such as hydroxyapatite or tricalcium phosphate. Due to their chemical similarity to an inorganic component of bone, hydroxyapatite, as well as βTCP- and αTCP-based materials, show excellent biocompatibility and osteoconductivity.
Implantations in orthopedics are associated with a high risk of bacterial infections in the surgery area. Therefore, biomaterials containing antibacterial agents, such as antibiotics, bactericidal ions or nanoparticles have been intensively investigated. In this work, silver decorated β tricalcium phosphate (βTCP)-based porous scaffolds were obtained and coated with a biopolymer—poly(3-hydroxybutyrate)-P(3HB). To the best of our knowledge, studies using silver-doped βTCP and P(3HB), as a component in ceramic-polymer scaffolds for bone tissue regeneration, have not yet been reported. Obtained materials were investigated by high-temperature X-ray diffraction, X-ray fluorescence, scanning electron microscopy with energy dispersive spectroscopy, hydrostatic weighing, compression tests and ultrahigh-pressure liquid chromatography with mass spectrometry (UHPLC-MS) measurements. The influence of sintering temperature (1150, 1200 °C) on the scaffolds’ physicochemical properties (phase and chemical composition, microstructure, porosity, compressive strength) was evaluated. Materials covered with P(3HB) possessed higher compressive strength (3.8 ± 0.6 MPa) and surgical maneuverability, sufficient to withstand the implantation procedures. Furthermore, during the hydrolytic degradation of the composite material not only pure (R)-3-hydroxybutyric acid but also its oligomers were released which may nourish surrounding tissues. Thus, obtained scaffolds were found to be promising bone substitutes for use in non-load bearing applications
2. Calcium Phosphate
The entry is from 10.3390/ma14154227
- Yoshida, K.; Kondo, N.; Kita, H.; Mitamura, M.; Hashimoto, K.; Toda, Y. Effect of substitutional monovalent and divalent metal ions on mechanical properties of β-tricalcium phosphate. J. Am. Ceram. Soc. 2005, 88, 2315–2318.
- Matsumoto, N.; Sato, K.; Yoshida, K.; Hashimoto, K.; Toda, Y. Preparation and characterization of β-tricalcium phosphate co-doped with monovalent and divalent antibacterial metal ions. Acta Biomater. 2009, 5, 3157–3164.
- Bohner, M.; Santoni, B.L.G.; Döbelin, N. β-tricalcium phosphate for bone substitution: Synthesis and properties. Acta Biomater. 2020, 113, 23–41.
- Honda, M.; Kawanobe, Y.; Nagata, K.; Ishii, K.; Matsumoto, M.; Aizawa, M. Bactericidal and bioresorbable calcium phosphate cements fabricated by silver-containing tricalcium phosphate microspheres. Int. J. Mol. Sci. 2020, 21, 3745.
- Su, Y.; Champagne, S.; Trenggono, A.; Tolouei, R.; Mantovani, D.; Hermawan, H. Development and characterization of silver containing calcium phosphate coatings on pure iron foam intended for bone scaffold applications. Mater. Des. 2018, 148, 124–134.
- Siek, D.; Ślósarczyk, A.; Przekora, A.; Belcarz, A.; Zima, A.; Ginalska, G.; Czechowska, J. Evaluation of antibacterial activity and cytocompatibility of α-TCP based bone cements with silver-doped hydroxyapatite and CaCO3. Ceram. Int. 2017, 43, 13997–14007.
- Hoover, S.; Tarafder, S.; Bandyopadhyay, A.; Bose, S. Silver doped resorbable tricalcium phosphate scaffolds for bone graft applications. Mater. Sci. Eng. C 2017, 79, 763–769.
- Dastidar, D.G.; Ghosh, D. Silver Nanoparticle Decorated Chitosan Scaffold for Wound Healing and Tissue Regeneration. Macromolecules 2018, 105, 1241–1249.
- Hasan, A.; Waibhaw, G.; Saxena, V.; Pandey, L.M. Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications. Int. J. Biol. Macromol. 2018, 111, 923–934.
- Busuioc, C.; Nicoara, A.I. Bacterial cellulose hydroxyapatite composites decorated with silver nanoparticles for medical applications. Eng. Biomater. 2019, 22, 153.
- Torre, E.; Giasafaki, D.; Steriotis, T.; Cassinelli, C.; Morra, M.; Fiorilli, S.; Vitale-Brovarone, C.; Charalambopoulou, G.; Iviglia, G. Silver decorated mesoporous carbons for the treatment of acute and chronic wounds, in a tissue regeneration context. Int. J. Nanomed. 2019, 14, 10147.
- Philippart, A.; Boccaccini, A.R.; Fleck, C.; Schubert, D.W.; Roether, J.A. Toughening and functionalization of bioactive ceramic and glass bone scaffolds by biopolymer coatings and infiltration: A review of the last 5 years. Expert Rev. Med. Devices 2015, 12, 93–111.
- Dziadek, M.; Zima, A.; Cichoń, E.; Czechowska, J.; Ślósarczyk, A. Biomicroconcretes based on the hybrid HAp/CTS granules, α-TCP and pectins as a novel injectable bone substitutes. Mater. Lett. 2020, 265, 127457.
- Cichoń, E.; Haraźna, K.; Skibiński, S.; Witko, T.; Zima, A.; Ślósarczyk, A.; Zimowska, M.; Witko, M.; Leszczynski, B.; Wrobel, A.; et al. Novel bioresorbable tricalcium phosphate/polyhydroxyoctanoate (TCP/PHO) composites as scaffolds for bone tissue engineering applications. J. Mech. Behav. Biomed. Mater. 2019, 98, 235–245.
- Skibiński, S.; Cichoń, E.; Haraźna, K.; Marcello, E.; Roy, I.; Witko, M.; Slosarczyk, A.; Czechowska, J.; Guzik, M.; Zima, A. Functionalized tricalcium phosphate and poly (3-hydroxyoctanoate) derived composite scaffolds as platforms for the controlled release of diclofenac. Ceram. Int. 2021, 47, 3876–3883.
- Ray, S.; Patel, S.K.; Singh, M.; Singh, G.P.; Kalia, V.C. Exploiting polyhydroxyalkanoates for tissue engineering. In Biotechnological Applications of Polyhydroxyalkanoates; Springer: Singapore, 2019; pp. 271–282.
- Peptu, C.; Kowalczuk, M. Biomass-derived polyhydroxyalkanoates: Biomedical applications. In Biomass as Renewable Raw Material to Obtain Bioproducts of High-Tech Value; Elsevier: Amsterdam, The Netherlands, 2018; pp. 271–313.
- Alves, M.I.; Macagnan, K.L.; Rodrigues, A.A.; de Assis, D.A.; Torres, M.M.; de Oliveira, P.D.; Furlan, L.; Vendruscolo, C.T.; Moreira, A.D.S. Poly (3-hydroxybutyrate)-P (3HB): Review of production process technology. Ind. Biotechnol. 2017, 13, 192–208.
- Koller, M. A review on established and emerging fermentation schemes for microbial production of Polyhydroxyalkanoate (PHA) biopolyesters. Fermentation 2018, 4, 30.
- Steinbüchel, A.; Valentin, H.E. Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiol. Lett. 1995, 128, 219–228.
- Zinn, M.; Witholt, B.; Egli, T. Occurrence, synthesis and medical application of bacterial polyhydroxyalkanoate. Adv. Drug Deliv. Rev. 2001, 53, 5–21.
- Singh, A.K.; Srivastava, J.K.; Chandel, A.K.; Sharma, L.; Mallick, N.; Singh, S.P. Biomedical applications of microbially engineered polyhydroxyalkanoates: An insight into recent advances, bottlenecks, and solutions. Appl. Microbiol. Biotechnol. 2019, 103, 2007–2032.
- Ali, I.; Jamil, N. Polyhydroxyalkanoates: Current applications in the medical field. Front. Biol. 2016, 11, 19–27.
- Sanhueza, C.; Acevedo, F.; Rocha, S.; Villegas, P.; Seeger, M.; Navia, R. Polyhydroxyalkanoates as biomaterial for electrospun scaffolds. Int. J. Biol. Macromol. 2019, 124, 102–110.
- Philip, S.; Keshavarz, T.; Roy, I. Polyhydroxyalkanoates: Biodegradable polymers with a range of applications. J. Chem. Technol. Biotechnol. Int. Res. Process Environ. Clean Technol. 2007, 82, 233–247.
- Peng, S.W.; Guo, X.Y.; Shang, G.G.; Li, J.; Xu, X.Y.; You, M.L.; Li, P.; Chen, G.Q. An assessment of the risks of carcinogenicity associated with polyhydroxyalkanoates through an analysis of DNA aneuploid and telomerase activity. Biomaterials 2011, 32, 2546–2555.
- Montazeri, M.; Karbasi, S.; Foroughi, M.R.; Monshi, A.; Ebrahimi-Kahrizsangi, R. Evaluation of mechanical property and bioactivity of nano-bioglass 45S5 scaffold coated with poly-3-hydroxybutyrate. J. Mater. Sci. Mater. Med. 2015, 26, 62.