1000/1000
Hot
Most Recent
The antioxidant, antimicrobial, and immunomodulatory activities of the Cleome droserifolia (Forssk.) Del. (Cd) shrub were investigated considering the biological activity of its phytogenic compounds.
Analysis | Content |
---|---|
Total phenols (mean ± SE, mg GA equivalent/g DM) | 32.55 ± 0.23 |
Total flavonoids (mean ± SE, mg CAT equivalent/g DM) | 12.78 ± 0.19 |
Individual detected phenolic compounds (µg/g DM) | |
Benzoic acid | 7657.15 |
Rutin | 2987.63 |
Ellagic acid | 1641.98 |
Naringenin | 1516.25 |
o-Coumaric acid | 1460.62 |
Rosmarinicacid | 955.27 |
p-Hydroxybenzoic acid | 924.57 |
Resveratrol | 895.77 |
Kaempferol | 778.80 |
Quercetin | 432.14 |
Ferulic acid | 264.06 |
Caffeic acid | 59.59 |
p-Coumaric acid | 39.55 |
Chlorogenic acid | 29.33 |
Syringic acid | 19.29 |
Catechin | 10.43 |
Antioxidant Concentration (µg/mL) | DPPH Scavenging Activity, % | ABTS Scavenging Activity, % | ||
---|---|---|---|---|
Cd Extract | Ascorbic Acid | Cd Extract | Ascorbic Acid | |
7.81 | 12.87± 0.91 b | 62.66 ± 0.12 a | 23.16 ± 0.76 b | 64.58 ± 0.24 a |
15.6 | 16.03 ± 0.84 b | 75.68 ± 0.45 a | 25.09 ± 0.84 b | 76.10 ± 0.92 a |
31.25 | 26.88 ± 0.86 b | 77.6 ± 0.86 a | 34.16 ± 0.92 b | 80.21 ± 1.04 a |
62.5 | 31.45 ± 1.12 b | 79.11 ± 1.14 a | 43.79 ± 1.16 b | 82.30 ± 0.86 a |
125 | 34.56 ± 1.24 b | 80.20 ± 0.88 a | 49.28 ± 1.13 b | 85.12 ± 0.45 a |
250 | 46.87 ± 1.16 b | 83.2 ± 0.62 a | 54.03 ± 0.76 b | 88.07 ± 0.93 a |
500 | 53.16 ± 0.85 b | 85.4 ± 0.56 a | 64.51 ± 0.85 b | 89.02 ± 0.88 a |
1000 | 66.09 ± 1.92 b | 87.52 ± 0.62 a | 81.14 ± 1.26 b | 92.44 ± 0.14 a |
Half-maximal inhibitory concentration | ||||
(IC50) (μg/mL) | 470.27 ± 2.24 a | 16.62 ± 0.91 b | 387.53 ± 3.11 a | 14.03 ± 0.67 b |
Pathogens Microorganisms | Inhibition Zone (mm) |
---|---|
Staphylococcus aureus NCTC 10788 | 15.63 ± 1.30 a |
Salmonella senftenberg ATCC 8400 | 12.70 ± 0.81 a |
Escherichia coli BA 12296 | 8.06 ± 1.72 b |
Candida albicans ATCC MYA-2876 | 7.16 ± 2.92 b |
Listeria monocytogenes ATCC 19116 | NI |
Treatment | Variable (Mean± Standard Error of the Mean, n = 10/Treatment) | |||
---|---|---|---|---|
Body Weight, g | Feed Intake, g/day | Fecal Score | Rectal Temperature, °C | |
C | 1454 ± 37.01 | 99.47 ± 17.36 | 1.19 ± 0.083 | 39.07 ± 0.112 a |
Cdl | 1413 ± 33.75 | 100.06 ± 15.88 | 1.21 ± 0.073 | 38.80 ± 0.091 b |
Cdh | 1393 ± 37.82 | 99.14 ± 17.31 | 1.13 ± 0.063 | 38.74 ± 0.123 b |
p-Value | 0.478 | 0.968 | 0.085 | 0.007 |
Treatment | Variable (Mean± Standard Error of the Mean, n = 6) | |||||||
---|---|---|---|---|---|---|---|---|
Red Blood Cell Count (106/mL) | Packed Cell Volume(%) |
Hemoglobin, g/dL | Total Protein, g/dL |
Albumin, g/dL |
Glucose, mg/dL |
Total Antioxidant Capacity, Mm/L |
Malondialdehyde, nmol/mL | |
At day 0 | ||||||||
C | 6.31 ± 1.01 | 32.67 ± 2.45 | 10.16 ± 0.32 | 6.34 ± 0.15 | 4.37 ± 0.11 | 93.61 ± 2.27 | 492.75 ± 0.53 | 5.23 ± 0.43 |
Cdl | 5.85 ± 1.09 | 33.05 ± 3.45 | 10.79 ± 0.58 | 6.72 ± 0.19 | 4.01 ± 0.07 | 91.51 ± 1.15 | 425.40 ± 1.64 | 4.92 ± 0.06 |
Cdh | 6.36 ± 0.97 | 34.45 ± 3.47 | 10.58 ± 0.79 | 6.65 ± 0.27 | 4.08 ± 0.12 | 93.31 ± 1.24 | 430.23 ± 1.59 | 5.20 ± 0.13 |
p-Value | 0.764 | 0.947 | 0.764 | 0.742 | 0.641 | 0.369 | 0.4752 | 0.379 |
At day 30 | ||||||||
C | 5.94 ± 1.21 | 31.35 ± 3.72 | 10.91 ± 0.67 | 6.44 ± 0.23 | 4.37 ± 0.24 | 91.92 ± 1.01 | 440.40 ± 0.30 b | 4.19 ± 0.25 a |
Cdl | 5.85 ± 0.98 | 30.37 ± 1.99 | 10.91 ± 0.37 | 6.28 ± 0.24 | 4.59 ± 0.06 | 91.56 ± 1.62 | 444.09 ± 0.95 a | 3.83 ± 0.04 b |
Cdh | 5.61 ± 1.23 | 30.01± 2.01 | 9.86± 0.34 | 6.14 ± 0.11 | 4.38 ± 0.15 | 92.41 ± 0.97 | 443.37 ± 0.92 a | 3.73 ± 0.04 b |
p-Value | 0.967 | 0.641 | 0.143 | 0.281 | 0.287 | 0.258 | 0.034 | 0.002 |
Treatment | Variable (Mean± Standard Error of the Mean, n = 6) | ||||||||
---|---|---|---|---|---|---|---|---|---|
White Blood Cells, 103/mm3 |
Lymphocytes, % |
Neutrocytes, % |
Echinocytes, % |
Monocytes, % |
Phagocytic Index |
Phagocytic Activity, % | Lysozyme Activity, U/mL |
Interleukin-β1, pg/mL | |
At day 0 | |||||||||
C | 7.29 ± 1.26 | 39.90 ±1.28 | 38.85 ± 2.33 | 12.49 ± 0.78 | 13.18 ± 2.00 | 1.94 ± 0.27 | 24.90 ± 1.24 | 0.113 ± 0.37 | 16.91 ± 0.34 |
Cdl | 6.47 ± 0.88 | 38.88 ± 1.91 | 33.75 ± 5.29 | 10.79 ± 1.18 | 11.65 ± 2.61 | 2.04 ± 0.13 | 19.39 ± 0.80 | 0.092 ± 0.01 | 15.21 ± 0.72 |
Cdh | 6.33 ± 1.42 | 38.71 ± 1.99 | 37.59 ± 3.32 | 10.12 ± 1.09 | 12.91 ± 0.69 | 1.96 ± 0.41 | 20.95 ± 0.12 | 0.101 ± 0.01 | 15.74 ± 0.82 |
p-Value | 0.560 | 0.240 | 0.338 | 0.327 | 0.679 | 0.804 | 0.258 | 0.175 | |
At day 30 | |||||||||
C | 6.49 ± 0.84 | 39.56 ± 1.32 | 32.69 ± 1.35 | 11.74 ± 0.52 | 13.22 ± 1.20 | 2.10 ± 0.35 | 20.56 ± 1.63 | 0.104 ± 0.02 b | 18.66 ± 0.22 a |
Cdl | 6.33 ± 0.56 | 42.01 ± 1.68 | 33.65 ± 3.05 | 10.22 ± 0.65 | 11.28 ± 1.53 | 2.19 ± 0.54 | 20.63 ± 1.01 | 0.106 ± 0.12 b | 17.01 ± 0.81 ab |
Cdh | 6.02 ± 1.40 | 44.52 ± 1.21 | 37.63 ± 1.92 | 11.51 ± 0.89 | 10.97 ± 1.37 | 2.49 ± 0.24 | 21.2 ± 2.01 | 0.142 ± 0.01 a | 15.25 ± 0.92 b |
p-Value | 0.449 | 0.123 | 0.236 | 0.531 | 0.195 | 0.446 | 0.561 | 0.046 | 0.001 |
Treatment | Variable (Mean± Standard Error of the Mean, n = 6) | ||
---|---|---|---|
IgG, mg/dL | IgA, mg/dL | IgE, mg/dL | |
At day 0 | - | ||
C | 981.32 ± 6.65 | 84.77 ± 2.68 | 7.73 ± 1.35 |
Cdl | 989.90 ± 10.41 | 85.79 ± 4.82 | 6.69 ± 0.67 |
Cdh | 985.74 ± 8.83 | 88.47 ± 3.45 | 7.99 ± 0.49 |
p-Value | 0.516 | 0.329 | 0.1602 |
At day 30 | - | ||
C | 974.57 ± 3.84 b | 91.78 ± 2.39 | 7.99 ± 0.78 |
Cdl | 987.91 ± 6.01 a | 93.86 ± 2.78 | 6.88 ± 0.38 |
Cdh | 982.99 ± 7.48 a | 93.06 ± 4.26 | 7.73 ± 0.28 |
p-Value | 0.016 | 0.647 | 0.359 |
Treatment | Variable (Mean± Standard Error of the Mean, n = 6) | |||
---|---|---|---|---|
Yeast | Lactobacillus | Salmonella | Coliform | |
Intestinal microflora (log cfu/g) | ||||
C | 4.83 ± 0.65 b | 6.80 ± 0.91 a | 5.96 ± 0.55 a | 6.30 ± 0.70 a |
Cdl | 7.60 ± 0.52 a | 8.10 ± 0.94 a | 3.10 ± 0.65 b | 4.86 ± 0.77 a |
Cdh | 8.06 ± 0.66 a | 8.06 ± 0.70 a | 3.13 ± 0.85 b | 4.83 ± 0.85 a |
Cecal microflora (log cfu/g) | ||||
C | 3.56 ± 0.81 b | 5.40 ± 0.55 a | 7.63 ± 0.86 a | 8.13 ± 0.61 a |
Cdl | 5.60 ± 1.13 a | 6.57 ± 1.70 a | 5.50 ± 0.45 b | 6.06 ± 1.30 b |
Cdh | 5.27 ± 0.83 ab | 6.93 ± 1.53 a | 5.34 ± 0.67 b | 6.20 ± 0.79 b |