Time-Lapse of Live Developmental Apoptosis in C.elegans Embryogenesis
  • View Times: 127
  • |
  • Release Date: 2021-12-06
  • Apoptosis
  • Development
  • embryogenesis
  • Caenorhabditis elegans
  • Cell Death
  • Programmed Cell Death
Video Introduction

This video is adapted from 10.3390/ijms222212466

Using a differential interference contrast (DIC) microscope, which gives depth and contrast to transparent specimens, Sulston and Horvitz directly observed embryonic and post-embryonic development of the nematode Caenorhabditis elegans (C. elegans). Sulston and later Bob Horvitz identified the precise developmental map of C. elegans, a journey from fertilized zygote to an adult worm, and mapped the first developmental lineage of a multicellular organism [1][2]. While analyzing the invariant C. elegans lineage, they made an intriguing observation, the temporally and developmentally regulated “programmed” cell death. It was evident that at specific developmental timing and based on certain developmental requirements, a number of cells died and disappeared surprisingly quickly. They interpreted the PCD as a series of morphological changes, especially in the cell nucleus, including bleb formation, refractivity increment, and finally cellular shrinkage and disappearance [3][1]. The initiation and execution of apoptosis occur in three main steps: cells have to decide to undergo programmed cell death, the execution of apoptosis has to be regulated, and, finally, cells undergoing apoptosis have to be marked and eliminated by phagocytosis.

References
  1. J.E. Sulston; H.R. Horvitz; Post-embryonic cell lineages of the nematode, Caenorhabditis elegans. Developmental Biology 1977, 56, 110-156, 10.1016/0012-1606(77)90158-0.
  2. J.E. Sulston; E. Schierenberg; J.G. White; J.N. Thomson; The embryonic cell lineage of the nematode Caenorhabditis elegans. Developmental Biology 1983, 100, 64-119, 10.1016/0012-1606(83)90201-4.
  3. John W. Saunders; Death in Embryonic Systems. Science 1966, 154, 604-612, 10.1126/science.154.3749.604.
Full Transcript
1000/1000

Confirm

Are you sure to Delete?
Cite
If you have any further questions, please contact Encyclopedia Editorial Office.
Izadi, M.; Ali, T.A.; Pourkarimi, E. Time-Lapse of Live Developmental Apoptosis in C.elegans Embryogenesis. Encyclopedia. Available online: https://encyclopedia.pub/video/video_detail/127 (accessed on 14 April 2024).
Izadi M, Ali TA, Pourkarimi E. Time-Lapse of Live Developmental Apoptosis in C.elegans Embryogenesis. Encyclopedia. Available at: https://encyclopedia.pub/video/video_detail/127. Accessed April 14, 2024.
Izadi, Mahmoud, Tayyiba Akbar Ali, Ehsan Pourkarimi. "Time-Lapse of Live Developmental Apoptosis in C.elegans Embryogenesis" Encyclopedia, https://encyclopedia.pub/video/video_detail/127 (accessed April 14, 2024).
Izadi, M., Ali, T.A., & Pourkarimi, E. (2021, December 06). Time-Lapse of Live Developmental Apoptosis in C.elegans Embryogenesis. In Encyclopedia. https://encyclopedia.pub/video/video_detail/127
Izadi, Mahmoud, et al. "Time-Lapse of Live Developmental Apoptosis in C.elegans Embryogenesis." Encyclopedia. Web. 06 December, 2021.