The physicochemical properties related to the environment behavior and fate of pollutants have been determined for 106 PCDE congeners by direct chromatographic methods (Table S2) [41,68]. However, given the time- and cost-consuming characteristics to evaluate the physicochemical properties experimentally as well as unavailability of standards for the remaining 103 PCDE congeners, various quantitative structure–property relationship (QSPR) methods have been developed and applied to predict the physicochemical properties based on diverse molecular structural descriptors and regression models. For example, seventeen theoretical molecular structural descriptors and partial least squares (PLS) regression were used to predict the P_L and n-octanol/water partition coefficient (K_OW) of 209 PCDE congeners [69]. Linear relationships were established between gas-chromatographic relative retention time (RRT), K_OW, PL and aqueous solubility (S_W,L) of PCDEs and some structural descriptors derived from molecular surface electrostatic potentials by a multiple linear regression (MLR) method and used to predict the physicochemical properties of PCDE congeners not determined experimentally [70]. QSPR models were developed by molecular electronegativity distance vector (MEDV-4) and MLR methods to estimate the P_L, K_OW and S_W,L of 209 PCDE congeners [71]. Based on the number of substituting Cl atoms on the different positions of parent compound diphenyl ether and the number of relative positions for these Cl atoms, a QSPR model was established by the theoretical linear solvation energy relationship (TLSER) method to predict the P_L of PCDEs with correlation coefficients R² of 0.991 [72]. An MLR approach was utilized to develop QSPR models to predict the P_L of 106 PCDEs based on calculated molecular descriptors [73]. The S_W,L values of five PCDE congeners were predicted using a PLS method [74]. The physicochemical properties predicted from the QSPR models mentioned above are listed in Table S3 [69,71,72,73,74]. The experimental and predicted results show that logP_L, logK_ow and logS_w,l of PCDEs range from −5.97 to −0.27, 4.38 to 8.31 and −12.95 to −4.21, respectively. These physicochemical properties indicate that PCDEs tend to accumulate in environments rich in organic matter, such as soils, sediments and organisms.

To the best of our knowledge, only three studies are available on the levels of PCDEs in water. Samples from the contaminated area of Whitby Harbor and a bridge near the entrance to Pringle Creek on the north shore of Lake Ontario were analyzed; 45 PCDE congeners were found in the semi-permeable membrane device (SPMD) at total concentrations of 0.68–7.07 ng/L [11]. In China, 15 PCDE congeners were detected in surface water samples from the Nanjing section of the Yangtze River [13]. The total concentration ranged from 1150 to 1800 ng/L and 730 to 1300 ng/L during the low- and high-water periods, respectively, with CDE 30 being the dominant congener. In the next study by the same group, the total concentrations of the PCDE congeners ranged from 0.351 to 2.021 ng/L in surface water samples from Chaohu Lake and its eight main tributaries in China, with CDE 30 (20.63%), CDE 28 (9.78%) and CDE 37 (9.52%) as the major congeners [12]. In general, PCDEs with less substituted Cl atoms have lower logK_ow and relatively higher water solubility [41]. Therefore, lower chlorinated PCDEs, such as mono-, di- and tri-CDEs are more easily transferred to the aqueous phase than higher chlorinated congeners [12]. The presence of PCDEs in water may be associated with surrounding or upstream industrial production and human activities, such as the production and use of chlorophenols, clofibrate, triclosan, bifenox, 2-chlorophenyl N-methylcarbamate and triadimefon [11,12,13,57]. Studies showed that CDE 37 and 77 could induce severe oxidative damage in green algae (Scenedesmus obliquus), water flea (Daphnia magna), zebrafish (Danio rerio) and crucian carp (Carassius auratus) at environmentally relevant concentrations [59,75].

PCDEs tend to accumulate in the sediment compared to water due to their higher hydrophobicity. The pollution of sediment by PCDEs was first reported for Whitby Harbour on the north shore of Lake Ontario in 1981 [76]. Subsequently, the environmental exposure of PCDEs has gradually received attention. The mean concentrations of total PCDE congeners in sediments of the contaminated area of Whitby Harbour were between 622 and 1929 ng/g dw in 1995 [11]. The average detection concentration of PCDEs in Lake Ontario was 1.30 ng/g dw, which was comparable to that of PCDDs (1.10 ng/g dw) and PCDFs (2.44 ng/g dw) [18]. In the sediment of Kymijoki River in Finland, which was highly contaminated by PCDEs due to the intensive production and use activities nearby of chlorophenol in the 19th century, the total concentration of PCDEs was determined in the range of approximately 130 to 554 ng/g dw (50 congeners tested) in 1993 [15], 8.79 to 606 ng/g dw (40 congeners tested), except for the reference sediment, in 1997 [16], and 85 ng/g dw (nine congeners tested) in 2001 [17]. The types and quantities of the measured compounds were different; therefore, it is difficult to judge the changing trend of PCDEs concentration in sediments of the Kymijoki River year by year. In industrially developed areas of eastern China, sediment samples were collected from Chaohu Lake and the Nanjing section of the Yangtze River, where the total concentrations of 15 PCDE congeners were in the range of 0.279–2.47 ng/g dw and 1.24–3.98 ng/g dw, respectively [12,13]. The level of PCDEs (mean: 1.30 ng/g dw) in the sediments of Chaohu Lake were higher than that of structurally similar polybrominated diphenyl ethers (PBDEs) tested (mean: 0.714 ng/g dw) [77], while lower than that of PCBs (mean: 12.07 ng/g dw) [78]. In addition to sediments, PCDEs in suspended particulate matter (SPM) of Chaohu Lake were also detected. The result showed that the mean total concentration of PCDEs in SPM was comparable to that in the sediment, which was 1.15 ng/g dw, lower than that of PBDEs (mean: 232.5 ng/g dw). In the SPM of the upper Narragansett Bay, the detected concentrations of tri-CDEs and tetra-CDEs were 0.03 ppt dw and 0.06 ppt dw, respectively, which were lower than that of tri-CDF (0.25 ppt dw) [19]. Furthermore, compared with the chlorinated degree of PCDEs in water, PCDEs with more chlorine atoms were more likely to accumulate in sediment and SPM.

By contrast, very little information is available on the levels of PCDEs in soils. An earlier study showed that the total concentration of 19 PCDE congeners ranged from <38 to 6800 ng/g dw in soils at 5 contaminated sawmill sites in Sweden [20].

Only one report to our knowledge has recently showed the levels of PCDEs in the atmosphere. That is, the atmospheric occurrence of six PCDE congeners were investigated over the rural area and the Pacific Ocean near Taiwan and the northern Philippines [21]. An elevated mean level of PCDEs was found in the ambient air of the rural area (0.014 pg/m³) compared with that found in the oceanic atmosphere (0.00875 pg/m³). CDE 28 was the predominant congener, accounting for 98.3 and 95.8% of the total PCDEs in the oceanic atmosphere and the ambient air over the land, respectively.

Organisms are susceptible to contamination by PCDEs in the environment due to their lipophilic nature. The presence of PCDEs in organisms was first identified in marine organisms, including clam (Mercenaria mercenaria), mussel (Mytilus edulis) and lobster (Honarus americanus), from Narragansett Bay in the United States [19]. PCDEs were also detected in freshwater fish in the North American Great Lakes. The total concentration of 28 monitored PCDE congeners ranged from 24 to 891 ng/g lw in lake trout (Sulvelinus namaycush) and walleye (Stizostedion vitreum vitreum) collected from the Great Lakes on a whole-fish basis [29]. Penta-, hexa- and hepta-chlorinated congeners were the most abundant homologue groups, representing approximately 80 to 90% of the total concentrations. In another study, the occurrence of 15 PCDE congeners was examined in whole fish samples of common carp (Cyprinus carpio) and northern pike (Esox lucius) caught from Whitby Harbour on the north shore of Lake Ontario [28]. The total levels of PCDEs varied from 768 to 14,005 ng/g ww, well above the detected concentrations of PCDFs (58–254 pg/g ww). In a later investigation on 8 fish species, including common shiner (Notropis cornutus), rosyface shiners (Notropis rubellus), spottail shiner (Notropis hudsonius), pumpkinseed (Lepomis gibbosus), yellow perch (Perca flavescens), brown bullhead (Ameiurus nebulosus), white sucker (Catostomus commersoni) and northern pike (Esox lucius), collected also from Whitby Harbour, the total lipid-normalized concentrations of 45 PCDE congeners in muscle samples for each species ranged from 100 to 2857 ng/g, 23231 to 43,231 ng/g, 20,706 to 96,529 ng/g, 30,417 to 68,250 ng/g, 4200 to 130,333 ng/g, 7538 to 213,231 ng/g, 16,714 to 174,571 and 21,000 to 47,000 ng/g, respectively [11]. CDE 99, 153 and 154 were the dominant congeners, and CDE 47, 74, 100, 118, 163, 182 and 184 were also significant. In addition to fish in inland lakes and coastal waters, PCDEs were also indirectly detected in deep sea fish through investigating levels of 106 PCDE congeners in 2 cod liver oils made from North Atlantic deep sea fish [9]. The total PCDE levels were 49 and 659 ng/g lw, respectively. These studies reflect the common presence of PCDEs in organisms in both marine and freshwater environments.

PCDEs have also been detected in organisms in other countries and regions. In oligochaete worm (Lumbriculus variegatus), chironomids and northern pike (Esox lucius) collected from sampling sites in the Kymijoki River in Finland, located downstream of an adjacent Ky-5 (which was a chlorophenol wood preservative) production plant, the total concentrations of 40 or 50 PCDE congeners were detected ranging from 215 to 1325 ng/g lw, 0 to 1200 ng/g lw and 677 to 706 ng/g lw, respectively [15,16]. The patterns of PCDE levels in these organisms were similar and resembled that in the sediments collected at the same sampling sites, and these dominant congeners were also abundant in Ky-5 as well. The major PCDE congeners detected in salmon from the Tenojoki Rive, Lake Saimaa and the Simojoki River in Finland were also similar and abundant in Ky-5 too [30]. Moreover, the congener patterns appear to be similar to those detected in Whitby Harbour fish [11]. It indicates that PCDEs contamination in the two regions may be attributed to the production or use of Ky-5 there.

PCDEs in organisms are acquired not only by bioconcentration from the ambient environment, but also by biomagnification throughout the food chain. PCDEs have been detected in birds and mammals that eat fish and other aquatic organisms. For example, eggs of fish-eating birds, including common tern (Sterna hirundo), black skimmer (Rynchops niger) and bald eagle (Haliaeetus leucocephalus) from Rhode Island, Louisiana, Michigan and Ohio were examined, and it was found that the total concentrations of three PCDE congeners tested ranged from 11 to 900 ng/g ww [24]. The high concentrations of PCDEs (sum of 7 congeners) were also found in eggs of black-crowned night herons (Nycticorax nycticorax) from Tianmu Lake and whiskered terns (Chlidonias hybrid) from East Tai Lake in China with levels ranging from 11 to 450 ng/g lw and 15 to 700 ng/g lw, respectively [26]. They were well above the detected total concentrations of PCDD/Fs of 0.38–19 and 2.6–33 ng/g lw in the two birds, respectively. In an investigation within the Baltic Sea area as the most polluted brackish water area in the world, the concentrations of individual PCDE congeners were detected ranging from <3 to 79 ng/g lw in eggs of black guillemots (Cepphus grylle L.) and from <5 to 13,000 ng/g lw in breast muscle of white-tailed sea eagles (Hallaeetus albicilla L.) as a top predator of the Baltic food chain [25]. The total concentrations of the 50 tested PCDE congeners varied from 233 to 354 ng/g lw and 1027 to 50,924 ng/g lw, respectively. They were also significantly higher than those of PCDD/Fs, i.e., 3.9–4.0 ng/g lw in black guillemots (Cepphus grylle L.) and 1.6–133 ng/g lw in white-tailed sea eagles (Hallaeetus albicilla L.). In mammals, such as seals, high levels of PCDEs were also detected. The contents of 50 individual congeners ranged from <0.3 to 62 ng/g lw in blubber of ringed seals (Phoca hispida botnica) and grey seals (Halichoerus grypus) from the Gulf of Finland in the Baltic Sea with the total concentrations of 39.9–373.9 ng/g lw [14]. PCDEs were at similar levels in the seal blubber compared to fish captured here and from the Kymijoki River that finally flows into the Gulf of Finland [16,27]. In blubber samples of a Baikal seal (Phoca sibirica) from Lake Baikal in East Siberia of Russia and several ringed seals (Phoca hispida saimensis) from Lake Saimaa in Southeast Finland, the total concentrations of the 50 congeners were found to be 60 ng/g lw and 217–459 ng/g lw, respectively [15]. In blubber samples of harbor seals (Phoca vitulina) captured from the Salish Sea in north–western North America, lower total contents of PCDE congeners (6.5–21 ng/g lw; sum of 46 congeners) were measured, which might be due to light PCDEs contamination in North America [33]. Furthermore, studies have demonstrated the presence of PCDEs in human adipose tissue. Tetra- to deca-CDE congeners in human adipose tissue collected from Canadian municipalities were analyzed. CDE 206 and 209 were found to be in the range of 0.1–2.9 ng/g lw, and the mean level of CDE 206 in males was greater than that in females [34]. Six hexa- to deca-CDE congeners were also detected in human adipose tissue from the USA, where the predominant congener was CDE 206 with concentrations ranging from 0.6 to 1.4 ng/g lw [35]. In addition, it was reported that the concentrations of 50 individual PCDE congeners varied between <0.5 and 7.9 ng/g lw in Finnish human adipose tissue, which were comparable to the levels of PCDD and PCDF congeners (<5 to 7700 pg/g lw) [35]. The main origin of PCDEs found in humans may be contaminated food. Human exposure to PCDEs through the diet was first reported in Catalonia (Spain) in 2004 [36]. PCDEs were detected in a number of foodstuffs available in the local market. The total PCDE concentrations in fresh hake (Rexea solandri), fresh sardine (Sardina pilchardus), mussels and tinned fish were 45.9–707, 400–2707, 59.8–107 and 3.3–71.9 pg/g ww, respectively. Total dietary intake of PCDEs through fish and shellfish was estimated to be 38 ng/day by a standard male adult of 70 kg body weight and aged between 20 and 65 years in Catalonia (Spain), which was slightly higher than PBDEs of approximately 31 ng/day. Moreover, PCDE intake was always higher in males than in females for people under 45 years old due to a greater food intake by males. In a subsequent study by the same research group, the concentrations of PCDEs were determined in 14 edible marine species widely consumed by the population of Catalonia (Spain) [38]. The highest PCDE levels (pg/g ww) were found in red mullet (Mullus barbatus; 7088) followed by sardine (Sardina pilchardus; 1829), anchovy (1606), tuna (Scombridae gen. sp.; 1292) and mackerel (1031). Children aged 4–9 years (boys 0.88 ng/kg/day and girls 0.73 ng/kg/day) showed the highest PCDE intake when judged by the average body weight [79]. Dietary intake of PCDEs in athletes was also evaluated [37]. In general, sportsmen and sportswomen showed a lower daily dietary intake than the general population due to ingesting lower amounts of fish and seafood. In another survey of PCDEs in foodstuffs in Catalonia (Spain) in 2006, the dietary intake of PCDEs was 51.68 ng/day for a standard male adult of 70 kg body weight, increasing by 26% compared to the previous survey (41 ng/day) in 2000, with fish and seafood being the main contributors to this increase [36,39]. In addition, the influence of different cooking processes including frying, grilling, roasting and boiling on the levels of PCDEs in various foodstuffs was evaluated. Studies showed that almost all cooking processes enhanced the total PCDEs levels in fish and meat samples [40,80]. Detailed information about the levels of PCDEs in various environmental media and biota reported previously is provided in Figure 3.