1. Introduction

According to the World Health Organization (WHO), more than 628 million confirmed cases of coronavirus disease 2019 (COVID-19) and approximately 6.5 million COVID‑19 deaths had been reported worldwide as of 1 November 2022 [1].

Various COVID-19 treatment strategies, including antiviral and immune therapy, have been developed and implemented during the pandemic [2]. However, vaccination is the primary method of combating the pandemic, with the goal of reducing hospitalizations, the proportion of severe COVID-19, and COVID-19 mortality. Because the SARS-CoV-2 virus has evolved significantly, vaccines must now develop a broader spectrum of immune defense mechanisms that target both currently circulating strains and new variants of the virus. The Technical Advisory Group on COVID-19 Vaccine Composition suggests including SARS-CoV-2 Omicron variant antigens in vaccine booster doses [3].

COVID-19 vaccines had been administered in a total of 12,449,443,718 doses as of 30 August 2022 [1].

Approximately 67.6% of the world’s population has received at least one dose of the vaccine, with 5.1 million doses being administered daily. Portugal and Cuba rank first in terms of the percentage of fully vaccinated people (95% each). The vaccination rate in low-income countries is only 20.9% [4].

As of 30 August 2022, Gibraltar (355.75 vaccine doses per 100 people), Chile (321.87 vaccine doses per 100 people), and Cuba (328.82 vaccine doses per 100 people) ranked first in terms of vaccine doses per person. According to WHO statistics, Gibraltar had 107 COVID‑19 deaths; Chile had 60,435; and Cuba had 8530 [5].

The vaccination of children and adolescents, the elderly, and pregnant women becomes increasingly important as time passes, as do determining the most effective vaccines and monitoring their efficacy to keep up with new virus mutations. This review discusses existing vaccine platforms and the main data available on the clinical use of vaccines in order to prioritize research.

This is an update to our previous review, which was published in December 2021 [6].

2. COVID-19 Vaccine Platforms and Their Characteristics

As of 30 August 2022, the WHO reported that 198 vaccines were in preclinical trials and 170 vaccines were in clinical trials. Forty-five vaccines are currently in phase 3 clinical trials, with eleven being already in phase 4. In total, 23% are single-dose vaccines, while 56% are double-dose vaccines, and 1% are triple-dose vaccines. In total, 3% of vaccines are intended for oral administration, 8% for subcutaneous and intradermal administration, 7% for intranasal administration, and 1% for use as aerosols or inhalers. The intramuscular route is the most commonly used (82%) for vaccine administration [7].

This review focuses solely on vaccines in clinical development. Figure 1 shows vaccine development platforms [7]. The most common vaccines in development are protein subunit (PS) vaccines, followed by ribonucleic acid (RNA) vaccines. These are followed by inactivated virus (IV) and non-replicating viral vector (VVnr) vaccines. Furthermore, replicating viral vector (VVr), virus‑like particle (VLP), live attenuated virus (LAV), and bacterial antigen-spore expression vector (BacAg-SpV) vaccines are being developed (Figure 1) [7].

Figure 1. Proportion of vaccine platforms in clinical development [7]. Notes: PS, protein subunit; VVnr, viral vector non-replication; DNA, deoxyribonucleic acid; IV, inactivated virus; RNA, ribonucleic acid; VVr, viral vector replicating; VLP, virus-like particle; APC, antigen presentation cell; LAV, live attenuated virus; BacAg-SpV, bacterial antigen-spore expression vector.

The highest numbers of vaccines are used in Iran (12 vaccines) and in the Philippines, India, and Indonesia (11 vaccines each), and the highest numbers of vaccine trials are currently underway in the United States (104 vaccines), China (98 vaccines), and India (36 vaccines). A total of 6 vaccines have been approved in Russia, and 33 are being studied (11 in phase 1 trials, 14 in phase 2 trials, and 8 in phase 3 trials) (Table 1) [8].

Table 1. Countries with the most vaccine research and/or the greatest numbers of vaccine registrations [8].

2.1. Protein Subunit (PS) Vaccines

This is the primary platform for many vaccines, both approved and in development [9]. Protein subunits are responsible for the formation of neutralizing antibodies as well as the activation of T cells [10]. PS-based vaccines are gaining popularity due to their ability to elicit stronger immune responses based on neutralizing antibodies using only immunodominant epitopes rather than larger viral proteins or whole inactivated virus [11]. The absence of serious side effects is one of the benefits of vaccines on this platform [12]. COVID-19 vaccine protein subunits do not contain live viruses; thus, they cannot cause infection [13]. Disadvantages include the need for revaccination, as well as the use of booster doses to maintain a stronger and longer immune response. Another disadvantage is that the vaccine’s effect is dependent on the adjuvant [12].

The entire S protein (BNT162b2/COMIRNATY Tozinameran (INN) (Pfizer, USA (BNT162b2)); NVX-CoV2373/Covovax/Nuvaxovid/Novavax (Novavax, India (NVX-CoV2373)); MVC-COV1901 (Medigen, Germany (MVC-COV1901)); mRNA-1273/Spikevax (Moderna, USA (mRNA‑1273)); and EpiVacCorona (Vector State Research Center of Virology and Biotechnology, Russia)), its receptor-binding domain (RBD) (Abdala, CIGB, Cuba (Abdala)), which is responsible for the binding of the virus to the angiotensin-converting enzyme 2 (ACE) receptor, and the N‑terminal domain (NTD) are primarily used in the development of COVID-19 vaccines [14,15].

According to the WHO, there were 22 protein subunit vaccines in phase 2/3–3 clinical trials and 1 in phase 4 clinical trials as of August 2022. The majority of existing vaccines were developed on this platform [9].

Several peptide-based vaccines are currently in phase 3 clinical trials, e.g., SARS-CoV-2 rS/Matrix M1-Adjuvant, Novavax, Sweden, Abdala, and EpiVacCorona [11]. However, EpiVacCorona production in Russia was halted in 2022, and the production of the AURORA‑CoV/EpiVacCorona-N vaccine began (Vector State Research Center of Virology and Biotechnology, Russia (AURORA-CoV)) [16,17].

2.2. Vaccines Containing Non-Replicating Viral Vector (VVnr)

VVnr vaccines contain SARS-CoV-2 genetic material encapsulated in a viral (vector) envelope; the vector can enter human cells but cannot replicate there. Vectors include adenoviruses, measles, smallpox, and vesicular stomatitis viruses. The advantage of such vaccines is that they produce long-lasting immunity. Because of the mucosal tropism of the virus, adenovirus vector-based vaccines can be administered intranasally [18].

At the time of this review, all registered VVnr vaccines were based on one or more of the adenoviral vectors listed below:

• Chimpanzee adenovirus vector ChAdOx1 (formerly known as ChAdY25). The AZD1222/Vaxzevria/Covishield vaccine (AstraZeneca, UK (AZD1222)) is based on this vector [17];
• Recombinant human adenovirus type 26 (Ad26) vector, contained in the Ad26.COV2.S/Jcovden (Janssen Pharmaceutical/Johnson & Johnson, Belgium/the Netherlands (Ad26.COV2.S)). It is recommended for people over the age of 18 who do not have access to other vaccines [18,19]. The same vector is used in Sputnik Light, (Gamaleya National Center of Epidemiology and Microbiology, Russia (Sputnik Light)). The Sputnik Light vaccine has the advantage of forming strong immunity after a single injection, can be used as a booster, and is effective against the Delta and Omicron strains [20];
• The Ad5-nCoV vaccine/Convidicea (CanSino Biologics, China (Ad5-nCoV)) is based on an adenovirus type 5 (Ad5) vector [21];
• The Sputnik V/Gam-COVID-Vac vaccine (Gamaleya National Center of Epidemiology and Microbiology, Russia (Sputnik V)) contains Ad26 and Ad5 vectors [18,20].

Except for Ad26.COV2.S and Sputnik Light, which are given only once, all the vaccines mentioned above are given twice (i.e., two doses of vaccine are required for full vaccination). All vaccine vectors contain a viral RNA fragment with the viral spike (S) protein, which mediates virus binding and translocation into the cell. As a result, the SARS-CoV-2 antigen is synthesized in human cells following vaccination [22]. Even a single injection of VVnr vaccine is immunogenic enough to produce protective antibody titers [23]. This enables the development and approval of single-phase vaccines. The main issue with VVnr is the retention of immunogenicity after repeated administration; the solution was found in vector alternation and the use of pegylated forms [24]. The effect of polyethylene glycol (PEG) on post-vaccination immunity is debatable. Recent research has found that anti-PEG antibodies have no effect on the specific neutralizing response of anti-SARS-CoV-2 antibodies after vaccination, but that high levels of anti-PEG vaccine-induced antibodies correlate with increased systemic reactogenicity after two doses of the vaccine [25,26].

2.3. DNA Vaccines

Nucleic acid vaccines work by introducing genetic material encoding immunogenic virus fragments into human cells. Following the delivery of the genetic material (RNA or deoxyribonucleic acid (DNA)), viral proteins are synthesized, which initiates the immune response and the production of antibodies against the virus. When DNA vaccines are used, the DNA is transcribed into messenger RNA upon reaching the host cells, which is then followed by protein synthesis [27]. DNA vaccines stimulate CD8 and CD4 T cells, eliciting both humoral and cellular immune responses [11]. DNA vaccines have several advantages, including rapid development and exceptional safety [28]. The disadvantages of DNA vaccines over RNA vaccines include the need for additional stages in the formation of an immune response [29]. Two vaccines are currently registered: NO-4800 (Inovio Pharmaceuticals, USA (NO-4800) [21]) and ZyCoV-D (Zydus Cadila, India (ZyCoV-D) [9]).

2.4. Vaccines Containing Inactivated Virus

Vaccines on this platform are popular due to their quick ability to adjust vaccine composition when the virus strain mutates, as well as their ease of production in comparison with other types of vaccines. Furthermore, these vaccines are simple to mass-produce. The disadvantage of this platform is the possibility of infection spreading due to insufficient pathogen inactivation [30]. Another significant disadvantage of inactivated vaccines is that they may be less immunogenic than mRNA or vector vaccines [31]. The WHO recommends using this type of COVID-19 vaccine in the elderly, healthcare workers, and immunocompromised patients [32].

The following vaccines are already approved and in use: COVID-19 Vaccine (Vero Cell), Inactivated/Coronavac^TM (Sinovac, China (COVID-19 Vaccine (Vero Cell), Inactivated)); BBIBP-CorV/SARS-CoV-2 Vaccine (Vero Cell), Inactivated (lnCoV)/Covilo (Sinopharm/BIBP, China (BBIBP-CorV)); BBV152/SARS-CoV-2 Vaccine, Inactivated (Vero Cell)/COVAXIN (Bharat Biotech, India (BBV152)); KoviVac (Chumakov Federal Scientific Center for the Research and Development of Immunobiologicals, Russia (KoviVac)). Two doses of these vaccines are required, which is one of their distinguishing features [21]. Other examples include Turkovac (Health Institutes of Turkey, Turkey (Turkovac)), FAKHRAVAC/MIVAC (Organization of Defensive Innovation and Research, Iran (FAKHRAVAC)), QazVac/QazCovid-in (Research Institute for Biological Safety Problems (RIBSP), Kazakhstan (QazVac)), KCONVAC (Shenzhen Kangtai Biological Products Co, China (KCONVAC)), and COVIran Barekat (Shifa Pharmed Industrial Co, Iran (COVIran Barekat)). All recent vaccines have only been approved in 1–2 countries and are only available in those countries [9].

The vaccine on this platform, COVID-19 Vaccine (Vero Cell), Inactivated, is more effective after two doses than BNT162b2 in reducing hospitalizations by 33.1% (95% CI, 14.5–47.7), intensive care admissions by 47.2% (95% CI, 18.5–65.8), and mortality by 55.7% (95% CI, 32.5–70.0) [33].

2.5. RNA Vaccines

Unlike DNA vaccines, the use of an mRNA-based platform eliminates the stage of RNA transcription in human cells, which speeds up and simplifies antigen synthesis [27]. Because the alphavirus nsP1-4 protein is included in the original RNA or is present as a separate fragment, mRNA vaccines can self-actualize. The vaccine’s self-amplification results in a stronger immune response, allowing lower doses to be used [34]. This vaccine induces both cell-mediated and humoral immune responses [10].

RNA vaccines already approved by the WHO include mRNA-1273 and BNT162b2, [21], as well as TAK-919 (Moderna formulation) (has the same formula as mRNA-1273), registered only in Japan. BNT162b2 has the highest number of countries that have approved the vaccine (146 countries) [9].

2.6. Viral Vector (Replicating)

There are currently no registered vaccines on this platform. Two oral vaccines are currently being developed: Oral Salmonella-Based Vaccine Platform (Korea), based on Salmonella expressing a modified spike protein [35]; and B. subtilis oral vaccine (DreamTec Research Limited, China), expressing sRBD on the spore surface [36].

2.7. Pathogen-Specific aAPCs

APC-based vaccines contain laboratory dendritic cells (DCs) that have the viral antigen on their surface. SARS-CoV-2 causes DC dysfunction, making DCs a viable vaccine target. Furthermore, DC stimulation induces a stronger T cell response that lasts longer than the humoral response [37]. The latter two platforms have served as the foundation for Shenzhen Genoimmune Medical Institute vaccines [21], which are currently in clinical trials [38].

Table 2 shows the characteristics of the main anti-SARS-CoV-2 vaccines.

Table 2. Characteristics of the main anti-SARS-CoV-2 vaccines.

3. Various Vaccine Delivery (Administration) Methods

When analyzing the routes of administration of COVID-19 vaccines, the most common method is intramuscular, followed by intranasal, and finally intradermal (Table 3) [7].

Table 3. Variants of vaccine delivery (administration) methods [7].

Intranasal vaccine administration is a promising option. Because SARS-CoV-2 primarily affects the respiratory tract, including the lungs, inducing mucosal immunity is essential for protection. The formation of adaptive immunity at the site of antigen primary penetration (inductor site) without the inclusion of a draining lymph node or spleen is a feature of mucosal immune response development. The already-formed effector lymphocytes migrate to the mucosal effector site. After activation in the inductor site, mucosal T and B cells migrate through the lymph and blood to various effector sites, where they differentiate into Th effectors, cytotoxic CD8+ T lymphocytes, and plasma cells [56]. Mucosal immunity results in the production of specific IgA directly in the mucosa, as well as neutralizing IgG and a specific response of T cells, which reduces the replication of the SARS-CoV-2 virus in the nasal epithelium [57]. Nasal vaccines are being developed using vectors (adenovirus, Newcastle disease virus, influenza virus, parainfluenza, and respiratory syncytial virus), protein subunits, and live attenuated virus as platforms [58]. Nebulizers and pipettes are used to deliver the vaccine [41]. As of July 2022, three nasal vaccines were in phase 3 clinical trials: DelNS1-2019-nCoV-RBD-OPT1, СOVI-VAC, and BBV154 (Вharat Biotech International Limited, India (BBV154)) [7]. In 2022, Russia registered two nasal combination, two-component vector vaccines based on adenovirus types 26 and 5: Sputnik V [59] and Salnavac (JSC Generium, Russia (Salnavac)) [60].

The use of oral vaccines against COVID-19 is being considered; their advantage is that no injections are required; thus, no pain syndrome exists [61]. There are no registered oral vaccines on the market at the moment. A vector vaccine based on adenovirus type 5, VXA‑CoV2‑1 (Vaxart Inc, USA (VXA-CoV2-1)), which acts on spike (S) protein and nucleoprotein (NP) (N), is currently in a phase 2 trial [7,62,63]. Another oral vaccine, OraPro‑COVID-19™ (iosBio Pharma, UK (OraPro-COVID-19™)), based on a non-replicating adenovirus, is also currently in clinical trials [64].

4. Monoclonal Antibodies for the Prevention of COVID-19

Anti-SARS-CoV-2 monoclonal antibodies are widely used for the treatment of COVID-19 infection, with moderate to high efficacy against both SARS-CoV-2 initial lines (B.1.351, P1, and B.1.1.7) and the Omicron variant [21]. However, the FDA has approved several products containing a combination of anti-SARS-CoV-2 monoclonal antibodies (casirivimab and imdevimab) for COVID-19 pre-exposure prophylaxis [65,66].

5. Immunogenicity and Safety of Vaccines

Vaccine effectiveness is determined by the induction of both humoral and cellular immune responses. As a result, quantifying neutralizing antibodies and the activity of specific T cells is recommended to assess vaccine efficacy and post-vaccination immunity [56,67,68]. According to mathematical modeling, post-vaccination neutralizing antibody titers are linearly related to vaccine efficacy [69]. The level of expression of each SARS-CoV-2 protein correlates with the prevalence and extent of CD4+ T cell responses to SARS-CoV-2 [70].

The ELISPOT platform is used by many researchers to assess the response of T cells induced by vaccination. T cell immune response was formed as early as 10 days after Sputnik Light vaccination, according to ELISPOT technology [54]. The ELISPOT-based Tigra Test SARS‑CoV-2 kit detected T cell immune response 90 and 120 days after Sputnik V immunization, including in seronegative individuals [71].

The efficacy of the main vaccines against the Omicron strain is of interest in light of the ongoing mutation of the SARS-CoV-2 virus [72]. Omicron (B.1.1.529) avoids many of the neutralizing antibody responses elicited by vaccines due to multiple mutations. In contrast to humoral immunity, vaccine-induced cellular immunity is highly reactive against the Omicron strain [73].

Immunogenicity can have a direct impact on vaccine safety and tolerability. In clinical trials, the incidence of adverse events was significantly higher after mRNA and vector vaccine administration (local reactions in 40–89% of cases and systemic reactions in 44–86% of cases) than after inactivated vaccine administration (injection-site reactions in 5–23% of cases and systemic reactions in 4–18% of cases) [74].

According to a meta-analysis, adverse events such as fever are more common after receiving inactivated vaccines, while headache and muscle pain are more common after receiving adenovirus vector-based vaccines, and 56% of people who received mRNA vaccines reported fatigue [75].

When the incidence of serious adverse events associated with BNT162b2, Ad26.COV2.S, and mRNA-1273 vaccinations was examined, the BNT162b2 vaccine had the highest reported incidence of myocarditis. The Ad26.COV2.S vaccine was more frequently linked to the development of Guillain–Barré syndrome. Thrombotic complications occurred more frequently with BNT162b2 and Ad26.COV2.S vaccination than with mRNA-1273 vaccination [43].

6. Vaccinations of Various Populations

6.1. General Population

COVID-19 mRNA vaccines (BNT162b2 and mRNA-1273) are recommended by the US Centers for Disease Control and Prevention (CDC) for primary and booster vaccinations in all populations. Currently, primary vaccination with one type of vaccine is recommended [76]. The WHO considers the following vaccines to be safe and effective: AZD1222; Ad26.COV2.S; mRNA-1273; BNT162b2; BBIBP-CorV; COVID-19 Vaccine (Vero Cell), Inactivated; BBV152; and NVX-CoV2373 (all trade names). In a clinical setting, these vaccines are equivalent, and healthcare professionals should use whichever option is available [55]. In patients under 60 years old, Australian guidelines prefer BNT162b2, mRNA-1273, or Novavax vaccines over AZD1222 [77]. As of July 2022, ten vaccines had been approved for use in Russia. A long-acting combination monoclonal antibody (tixagevimab + cilgavimab) can be used for pre-exposure prophylaxis of COVID-19 in adults and children (aged 12 years and older weighing at least 40 kg) who are not infected with SARS-CoV‑2, have not been in contact with a person infected with SARS-CoV-2, and have contraindications for vaccination against COVID-19 [78].

6.2. Use in Pregnancy

The CDC and the WHO recommend any approved COVID-19 vaccination for those pregnant, breastfeeding, trying to get pregnant now, or planning to get pregnant in the future [55]. Russian national guidelines recommend Sputnik V vaccination for pregnant women if the benefit outweighs the risk. Vaccination is contraindicated while breastfeeding [79].

6.3. Children and Adolescents

The CDC recommends the COVID-19 vaccination for anyone aged 6 months and older. BNT162b2 and mRNA-1273 are approved for use in children [76]. The WHO recommends starting the BNT162b2 vaccine at the age of five, and the mRNA-1273 vaccine at the age of twelve [62]. Vaccination is recommended for everyone over the age of five in Australia, [63] and over the age of twelve in Canada [80]. In Russia, the Gam-COVID-Vac-M vaccine is administered to children aged 12 to 17 [78].

6.4. People Who Have Had COVID-19

The CDC recommends vaccination for all people over the age of six months, regardless of whether they have a history of symptomatic or asymptomatic SARS-CoV-2 infection [76]. The WHO also supports this strategy [55]. In Australia, vaccination is recommended no sooner than three months after infection confirmation [63]. In Russia, vaccination is recommended 6 months after the disease, based on epidemic indications (including in previously vaccinated individuals) [81].

6.5. Immunocompromised Patients

For immunocompromised patients, the CDC recommends COVID-19 mRNA vaccines (BNT162b2 or mRNA-1273) for primary and booster vaccinations [76]. In this population, the WHO and Australian guidelines recommend additional doses of the BNT162b2 or mRNA-1273 vaccine [55,77]. Given the risk of more severe infection, Canadian guidelines recommend vaccinating patients with primary immunodeficiency with mRNA vaccines [82]. In Russia, the Sputnik V vaccine is recommended in this population. People with immunodeficiency who have had COVID-19 may receive Sputnik Light [81].

6.6. Booster Vaccination

Booster vaccination and revaccination (seasonal vaccination) against COVID-19 are used to maintain active immunity against COVID-19 for a longer period of time after primary immunization. A booster is the administration of a vaccine with the same or a different antigenic composition before the previous vaccine’s immunogenic effect has worn off. The next booster dose of the vaccine should increase the intensity and duration of post-vaccination immunity. The timing of the booster vaccination ranges from three to six months, depending on the country’s legislative framework [77,81–84]. Revaccination (seasonal vaccination) against COVID-19 is performed 12 months after the main (primary) or booster vaccination [85].

As recently as late 2021, the need for booster doses of COVID-19 vaccines was debatable [6]. Lately, public opinion on vaccine booster administration has shifted in favor of mandatory administration of booster doses, as this increases immunity duration and resistance to the Omicron strain [40,86]. The CDC recommends a booster injection for patients over the age of 12, with the first booster being administered three months after primary immunization. In patients over the age of 50, the second booster should be administered four months after the first [76]. According to WHO recommendations, one booster dose is sufficient [55]. In Australia, a single dose of a COVID-19 vaccine booster is recommended for people aged 16 and older who have completed their primary course three months ago or more. The second booster is recommended for people over the age of 50, nursing-home residents, people with severe immunosuppression, people with significant disabilities, and people at risk of severe COVID-19 infection [77]. In Canada, booster doses are recommended from 18 years of age [82]. In the United Kingdom, booster immunization with BNT162b2 or mRNA-1273 is currently recommended three months after primary vaccination [84]. In Russia, vaccination with Sputnik Light, EpiVacCorona, and AURORA-CoV is recommended [81].

Some vaccines have only been approved for use as boosters. Only a booster dose of the BNT162b2 vaccine is recommended for adolescents aged 12 to 17 years [87]. In August 2022, the mRNA-1273.214/Moderna^TX vaccine was registered (Moderna, USA (mRNA-1273.214)). This is a bivalent mRNA vaccine containing sequences encoding SARS-CoV-2 and Omicron variant spike proteins [88]. A systematic review as of May 2022 found that three doses of RNA vaccines provided the best efficacy for vaccination and booster vaccination (96% vaccine efficacy; 95% CI, 72% to 99%). The combination of two adenovirus vector vaccines and one RNA vaccine has an efficiency of 88% (range: 59–97%). The homologous two-dose regimen of RNA vaccines results in 99% (79% to 100%) vaccine efficacy in preventing severe COVID-19. Triple-dose RNA vaccines are the most effective in reducing COVID-19-related hospitalizations (95% CI, 90% to 97%). Three-dose regimens of homologous and heterologous strains have been shown to be effective in preventing infection with the Alpha, Delta, and Omicron strains [89].

7. Vaccines and New Strains

The WHO has currently registered six SARS-CoV-2 variants of concern. Three pre-existing variants—Alpha, Beta, and Gamma—have already lost their relevance. Delta and Omicron are the two most important variants right now [90].

The SARS-CoV-2 Alpha variant includes viral strains with 23 mutations, including 7 mutations and 2 deletions in the RNA fragments encoding the S protein [91]. This variant is significantly more virulent, with a higher risk of death or severe COVID-19. The Beta variant mutations include nine mutations and one deletion in spike (S) protein [92]. The variant is distinguished by a significant acceleration of disease spread when compared with the reference strain [93]. In the Gamma variant of SARS-CoV-2, 12 mutations alter the structure of the S protein, increasing virulence. In the Delta variant, there are 19 known mutations in the S protein that alter the structure of the RBD of the S protein [91]. The Omicron strain has a high ability to mutate, resulting in the formation of numerous sublines, and new variants have significant genomic differences from the first lines [93]. The main problems with Omicron are that it is more infectious or dangerous than other variants of concern and that it can bypass vaccine-induced immunity. When tested in patients’ plasma after triple vaccination and subsequent infection with BA.1, subspecies BA.2.12.1, BA.4, and BA.5 showed higher transmissibility and lower antibody neutralizing ability than BA.2 [94]. The most recent SARS-CoV-2 mutations from the strain BA.2.75 subspecies, nicknamed “Centaurus” and “Pisces”, are thought to be more resistant to vaccine-induced immunity than previous strains [90,95].

The WHO recommends a vaccine efficacy threshold of 50% [96].

Neutralizing antibodies induced by BNT162b2 and mRNA-1273 are lower than those induced by P.1 and B.1.351. The Ad26.COV2.S vaccine was 64.0% effective in the Brazilian population and 52% effective in the South African population against variant B.1.351; NVX-CoV2373 was shown to be 49% effective in the South African population. The BNT162b2 vaccine was found to be effective against the P.1 variant. The efficacy of BNT162b2, mRNA-1273, AZD1222, and COVID-19 Vaccine (Vero Cell), Inactivated, against Gamma and Delta variants was estimated as 85%, 78%, 70%, and 66%, respectively. Two doses of the BNT162b2 vaccine were found to cross-neutralize some circulating Delta variants, while the Ad26.COV2.S vaccine’s efficacy against this virus variant was reduced from 66.9% to 60%. The mRNA-1273 vaccine was found to be approximately 94.1% effective against the Delta variant when compared with the BNT162b2 and Ad26.COV2.S vaccines, while the BNT162b2 and AZD1222 vaccines were less effective [97]. In an Indian study, BBV152 and AZD1222 were 93% and 94% effective, respectively, against the Delta strain [98]. The Sputnik V vaccine was 81% effective in preventing the hospitalization of patients with the Delta variant in preclinical studies [99].

BNT162b2 (35%), mRNA-1273 (20% after the first dose, 42.8% after the second dose, 67.7% after the third dose), Ad26.COV 2.S (47% after two doses, 63% when given as a booster), and AZD1222 (11.44–51%) all demonstrated activity against the Omicron strain [100,101]. Monoclonal antibodies are also active against Omicron [42].

The serum titer of neutralizing antibodies to the Omicron variant in patients revaccinated with Sputnik Light was statistically comparable to the serum titer of neutralizing antibodies to the B.1.1.1 variant in patients vaccinated with Sputnik V. Despite a decrease in titer, neutralizing antibodies to Omicron were detected in the serum of 100% of revaccinated individuals after Sputnik Light revaccination [49].

In Canada, mRNA-1273.214/Spikevax Bivalent Original/Omicron BA.1 (Moderna, USA (mRNA-1273.214)), containing mRNA encoding the original SARS-CoV-2 virus and Omicron BA.1, has been approved for use as a booster. When compared with mRNA-1273 as a second booster dose, this vaccine induces higher neutralizing antibody titers against the parent strain, as well as Omicron BA.1, Omicron BA.4, and Omicron BA.5 [102]. mRNA‑1273.222/Spikevax Bivalent Original/Omicron BA.4/BA.5 (Moderna, USA (mRNA‑1273.214)) has been approved in the USA [103].

Pfizer-BioNTech has launched two bivalent vaccines: BNT162b2 (B.1.1.529)/BNT162b2 Bivalent/Comirnaty Bivalent Original/Omicron BA.1, produced by Pfizer/BioNTech, USA (BNT162b2 (B.1.1.529)); and BNT162b2 Bivalent BA.4/BA.5/Comirnaty Bivalent Original/Omicron BA.4/BA.5, produced by Pfizer-BioNTech, USA (BNT162b2 Bivalent). Both vaccines contained one dose of Comirnaty and one dose of Omicron subvariant vaccine (BA.1 or BA.4/BA.5) [104].

Phase 3 studies are ongoing for Omicron COVID-19 Inactivated Vaccine (Vero Cell), produced by China National Biotec Group Company Limited, China (Omicron COVID-19 Inactivated Vaccine (Vero Cell)), an inactivated virus vaccine [7,105].

8. Immunization Schedule

The CDC has developed a COVID-19 vaccination schedule for children starting at 6 months of age, in which a booster is administered no later than 5 months after two primary doses of one vaccine, and people over 50 years of age should receive a second booster after 4 months [68].

In Belarus, revaccination (seasonal vaccination) with Sputnik V, Sputnik Light, BBIBP-CorV, or another immunobiological drug is recommended 12 months after the booster vaccination for persons 18 years of age and older and after the main (primary) vaccination for those who did not receive booster vaccination, during periods of seasonal increase in the incidence [85].

In Russia, citizens in three priority levels are subject to COVID-19 vaccination based on epidemic indications. The first level includes people aged 60 and older who work in service organizations, have chronic diseases, and live in large cities. The second level includes civil servants and employees of law enforcement and other bodies, as well as service workers. The third level includes children aged 12 to 17, pupils and students, army conscripts, and office workers [106].

9. Conclusions

The updated review reveals that, despite all efforts, the SARS-CoV-2 virus has not yet been eradicated. The virus’s ability to mutate indefinitely has resulted in the emergence of new strains that are resistant to previously acquired immunity and can bypass previously formed immune mechanisms.

This necessitates the development of new, more effective vaccines. The vaccine market is rapidly expanding, with new products appearing all the time. A total of 42 vaccines have already been approved for use. To date, the top three most influential COVID-19 vaccine platforms have not changed, with mRNA, VVnr, and inactivated virus vaccines maintaining their positions. However, focus has shifted to PS vaccines.

The development of COVID-19 vaccination has increased not only the availability of vaccines, but also the ease with which they can be used. For example, single-dose vaccines and nasal vaccines are already in use, and oral vaccines are being developed, which will undoubtedly simplify their administration.

New products, including those based on the inactivated Omicron virus, are being developed to improve the efficacy of vaccines against new strains of SARS-CoV-2. The approach to booster doses and revaccination has also been reconsidered. Currently, the use of one or even two booster vaccines is recommended, which does not eliminate the need for revaccination.

With the accumulation of experience in the use of COVID-19 vaccines, the number of people who can be vaccinated has increased while the list of contraindications has decreased. Vaccines are now known to be safe for children, pregnant women, the elderly, and people with immunosuppressive conditions. Vaccination is recommended even for people who have already had COVID-19.

The fast-paced process of combating SARS-CoV-2 is associated with the constant updating of available information, as well as the change of guidelines, which necessitates regular review as new changes emerge.

Author Contributions: Conceptualization, D.K., O.S., and A.S.; methodology, D.K. and A.S.; software, D.K. and O.S.; validation, D.K. and A.S.; formal analysis D.K. and A.S.; investigation, D.K. and A.S.; resources, D.K. and A.S.; data curation, D.K. and A.S.; writing—original draft preparation, D.K., O.S., and A.S.; writing—review and editing, D.K., O.S., and A.S.; visualization, D.K. and A.S.; supervision, A.S.; project administration, D.K., O.S., and A.S. All authors have read and agreed to the published version of the manuscript.

Funding: This research received no external funding.

Conflicts of Interest: The authors declare no conflicts of interest.

References

1. WHO Coronavirus (COVID-19) Dashboard. Available online: https://covid19.who.int (accessed on 1 November 2022).
2. Starshinova, A.; Malkova, A.; Zinchenko, U.; Kudlay, D.; Glushkova, A.; Dovgalyk, I.; Yablonskiy, P.; Shoenfeld, Y. Efficacy of Different Types of Therapy for COVID-19: A Comprehensive Review. Life 2021, 11, 753.
3. Interim Statement on the Composition of Current COVID-19 Vaccines. Available online: https://www.who.int/news/item/17-06-2022-interim-statement-on--the-composition-of-current-COVID-19-vaccines (accessed on 30 August 2022).
4. Mathieu, E.; Ritchie, H.; Rodés-Guirao, L.; Appel, C.; Giattino, C.; Hasell, J.; Macdonald, B.; Dattani, S.; Beltekian, D.; Ortiz-Ospina, E.; Roser, M. (2020) - "Coronavirus Pandemic (COVID-19)". Published online at OurWorldInData.org. Available online: https://ourworldindata.org/coronavirus (accessed on 30 August 2022).
5. Available online: https://covid19.who.int/table (accessed on 31 August 2022).
6. Kudlay, D.; Svistunov, A. COVID-19 Vaccines: An Overview of Different Platforms. Bioengineering 2022, 9, 72.
7. COVID-19 Vaccine Tracker and Landscape. Available online: https://www.who.int/publications/m/item/draft-landscape-of-covid-19-candidate-vaccines (accessed on 1 November 2022).
8. Vaccination Rates, Approvals & Trials by Country—COVID19 Vaccine Tracker. Available online: https://covid19.trackvaccines.org/trials-vaccines-by-country/ (accessed on 26 October 2022).
9. Vaccines—COVID19 Vaccine Tracker. Available online: https://covid19.trackvaccines.org/vaccines/approved/#vaccine-list (accessed on 31 August 2022).
10. Wambani, J.; Okoth, P. Scope of SARS-CoV-2 variants, mutations, and vaccine technologies. J. Intern. Med. 2022, 34, 34.
11. Rezaei, M.; Nazari, M. New Generation Vaccines for COVID-19 Based on Peptide, Viral Vector, Artificial Antigen Presenting Cell, DNA or mRNA. Avicenna J. Med. Biotechnol. 2022, 14, 30–36.
12. Junaidi, K; Fitrina, D.W.; Anggrainy, F.; Herman, D. Overview of COVID-19 Vaccine Development Strategy. Med. : J. Biomed. Transl. Res. 2022, 6, 1537–1559.
13. COVID-19 Vaccination. Centers for Disease Control and Prevention. 2020. Available online: https://www.cdc.gov/coronavirus/2019-ncov/vaccines/different-vaccines/proteinsubunit.html (accessed on 16 August 2022).
14. Pack, S.M.; Peters, P.J. SARS-CoV-2–Specific Vaccine Candidates; the Contribution of Structural Vaccinology. Vaccines 2022, 10, 236.
15. Ryzhikov, A.B.; Ryzhikov, E.A.; Bogryantseva, M.P.; Usova, S.V.; Danilenko, E.D.; Nechaeva, E.A.; Pyankov, O.V.; Pyankova, O.G.; Gudymo, A.S.; Bodnev, S.A.; et al. A single blind, placebo-controlled randomized study of the safety, reactogenicity and immunogenicity of the “EpiVacCorona” vaccine for the prevention of COVID-19, in volunteers aged 18-60 years (phase I-II). Инфекция И Иммунитет 2021, 11, 283–296.
16. In Russia, the Production of the EpiVacCoron Vaccine Has Ceased. RBC. Available online: https://nsk.rbc.ru/nsk/23/05/2022/628b36a29a7947b64836230c (accessed on 18 July 2022).
17. EpiVacCorona-N or Aurora-CoV? The Fifth Vaccine Against Coronavirus Appeared in Russia. PharmMedProm. Available online: https://pharmmedprom.ru/news/epivakkorona-n-ili-aurora-cov-v-rossii-poyavilas-pyataya-vaktsina-ot-koronavirusa/ (accessed on 30 August 2022).
18. Kharit, S.M.; Fridman, I.V. Efficacy and safety of vector vaccines for the prevention of new coronavirus infection. Infectology 2022, 14, 14–26. (In Russ) https://doi.org/10.22625/2072-6732-2022-14-2-14-26.
19. The Janssen COVID-19 Vaccine Emergency Use Authorization (EUA) Official Website. Available online: https://www.janssencovid19vaccine.com/hcp.html (accessed on 31 August 2022).
20. Sputnik Light Is an Approved One-Component Vaccine, Which Is the First Component of the Sputnik V Vaccine Against COVID-19, Developed by the N.F. Gamaleya with the Support of the Russian Direct Investment Fund in Russia. Available online: https://sputnikvaccine.com/rus/about-vaccine/sputnik-light/ (accessed on 18 July 2022).
21. Simões, R.S.Q.; Rodríguez-Lázaro, D. Classical and Next-Generation Vaccine Platforms to SARS-CoV-2: Biotechnological Strategies and Genomic Variants. J. Env. Res. Public Health 2022, 19, 2392.
22. Wu, S.; Huang, J.; Zhang, Z.; Wu, J.; Zhang, J.; Hu, H.; Zhu, T.; Zhang, J.; Luo, L.; Fan, P.; et al. Safety, tolerability, and immunogenicity of an aerosolised adenovirus type-5 vector-based COVID-19 vaccine (Ad5-nCoV) in adults: Preliminary report of an open-label and randomised phase 1 clinical trial. Lancet Infect. Dis. 2021, 21, 1654–1664.
23. Henao-Restrepo, A.M.; Camacho, A.; Longini, I.M.; Watson, C.H.; Edmunds, W.J.; Egger, M.; Carroll, M.W.; Dean, N.E.; Diatta , I.; Doumbia, M.; et al. Efficacy and effectiveness of an rVSV-vectored vaccine in preventing Ebola virus disease: Final results from the Guinea ring vaccination, open-label, cluster-randomised trial (Ebola Ça Suffit!). Lancet Lond. Engl. 2017, 389, 505–518.
24. Davidovic, T.; Schimpf, J.; Abbassi-Nik, A.; Stockinger, R.; Sprenger-Mähr, H.; Lhotta, K.; et al. Humoral and Cellular Immune Response After a 3-Dose Heterologous SARS-CoV-2 Vaccination Using the mRNA-BNT162b2 and Viral Vector Ad26COVS1 Vaccine in Hemodialysis Patients. Immunol. 2022, 13, 907615.
25. Ju, Y.; Lee, W.S.; Pilkington, E.H.; Kelly, H.G.; Li, S.; Selva, K.J.; et al. Anti-PEG Antibodies Boosted in Humans by SARS-CoV-2 Lipid Nanoparticle mRNA Vaccine. ACS Nano. 2022, 16, 11769–11780.
26. Padín-González, E.; Lancaster, P.; Bottini, M.; Gasco, P.; Tran, L.; Fadeel, B.; et al. Understanding the Role and Impact of Poly (Ethylene Glycol) (PEG) on Nanoparticle Formulation: Implications for COVID-19 Vaccines. Bioeng. Biotechnol. 2022, 10, 882363.
27. Pardi, N.; Hogan, M.J.; Porter, F.W.; Weissman, D. mRNA vaccines—A new era in vaccinology. Rev. Drug Discov. 2018, 17, 261–279.
28. Kyriakidis, N.C.; López-Cortés, A.; González, E.V.; Grimaldos, A.B.; Prado, E.O. SARS-CoV-2 vaccines strategies: A comprehensive review of phase 3 candidates. NPJ Vaccines 2021, 6, 28.
29. Mallapaty, S. India’s DNA COVID vaccine is a world first—More are coming. Nature 2021, 597, 161–162.
30. Dai, X.; Xiong, Y.; Li, N.; Jian, C. Vaccine Types. Vaccines—the History and Future. IntechOpen. 2019. Available online: https://www.intechopen.com/chapters/undefined/state.item.id (accessed on 31 August 2022).
31. Lim, W.W.; Mak, L.; Leung, G.M.; Cowling, B.J.; Peiris, M. Comparative immunogenicity of mRNA and inactivated vaccines against COVID-19. Lancet Microbe 2021, 2, e423.
32. The Sinovac-CoronaVac COVID-19 Vaccine: What You Need to Know. Available online: https://www.who.int/ru/news-room/feature-stories/detail/the-sinovac-covid-19-vaccine-what-you-need-to-know (accessed on 31 August 2022).
33. Paternina-Caicedo, A.; Jit, M.; Alvis-Guzmán, N.; Fernández, J.C.; Hernández, J.; Paz-Wilches, J.J.; Rojas-Suarez, J.; Dueñas-Castell, C.; Alvis-Zakzuk, N.J.; Smith, A.D.; et al. Effectiveness of CoronaVac and BNT162b2 COVID-19 mass vaccination in Colombia: A population-based cohort study. Lancet Reg. Health Am. 2022, 12, 100296.
34. Bloom, K.; van den Berg, F.; Arbuthnot, P. Self-amplifying RNA vaccines for infectious diseases. Gene Ther. 2021, 28, 117–129.
35. Yoon, W.; Park, Y.; Kim, S.; Bang, I.S. Development of an Oral Salmonella-Based Vaccine Platform against SARS-CoV-2. Vaccines 2022, 10, 67.
36. The Development of a COVID19 Oral Vaccine Consisting of Bacillus Subtilis Spores. Clinicaltrials.gov; Report No.: NCT05057923. Available online: https://clinicaltrials.gov/ct2/show/NCT05057923 (accessed on 18 November 2022).
37. Jonny, J.; Putranto, T.A.; Sitepu, E.C.; Irfon, R. Dendritic cell vaccine as a potential strategy to end the COVID-19 pandemic. Why should it be Ex Vivo? Expert Rev. Vaccines 2022, 21, 1111–1120.
38. Shenzhen Geno-Immune Medical Institute. Phase I/II Multicenter Trial of Lentiviral Minigene Vaccine (LV-SMENP) of Covid-19 Coronavirus. Clinicaltrials.gov; Report No.: NCT04276896. 2020. Available online: https://clinicaltrials.gov/ct2/show/NCT04276896 (accessed on 17 July 2022).
39. El Sahly, H.M.; Baden, L.R.; Essink, B.; Doblecki-Lewis, S.; Martin, J.M.; Anderson, E.J.; Campbell, T.B.; Clark, J.; Jackson, L.A.; Fichtenbaum, C.J.; et al. Efficacy of the mRNA-1273 SARS-CoV-2 Vaccine at Completion of Blinded Phase. Engl. J. Med. 2021, 385, 1774–1785.
40. Andrews, N.; Stowe, J.; Kirsebom, F.; Toffa, S.; Rickeard, T.; Gallagher, E.; Gower, C.; Kall, M.; Groves, N.; O’Connell, A.-M.; et al. Covid-19 Vaccine Effectiveness against the Omicron (B.1.1.529) Variant. Engl. J. Med. 2022, 386, 1532–1546.
41. Moreira, E.D.; Kitchin, N.; Xu, X.; Dychter, S.S.; Lockhart, S.; Gurtman, A.; Perez, J.L.; Zerbini, C.; Dever, M.E.; Jennings, T.W.; et al. Safety and Efficacy of a Third Dose of BNT162b2 Covid-19 Vaccine. Engl. J. Med. 2022, 386, 1910–1921.
42. Vitiello, A.; Ferrara, F.; Auti, A.M.; Di Domenico, M.; Boccellino, M. Advances in the Omicron variant development. Intern. Med. 2022, 292, 81–90.
43. Guo, W.; Deguise, J.; Tian, Y.; Huang, P.C.E.; Goru, R.; Yang, Q.; Peng, S.; Zhang, L.; Zhao, L.; Xie, J.; et al. Profiling COVID-19 Vaccine Adverse Events by Statistical and Ontological Analysis of VAERS Case Reports. Pharmacol. 2022, 13, 870599.
44. Sadoff, J.; Gray, G.; Vandebosch, A.; Cárdenas, V.; Shukarev, G.; Grinsztejn, B.; Goepfert, P.A.; Truyers, C.; Fennema, H.; Spiessens, B.; et al. Safety and Efficacy of Single-Dose Ad26.COV2.S Vaccine against Covid-19. Engl. J. Med. 2021, 384, 2187–2201.
45. Voysey, M.; Clemens, S.A.C.; Madhi, S.A.; Weckx, L.Y.; Folegatti, P.M.; Aley, P.K.; Angus, B.; Baillie, V.L.; Barnabas, S.L.; Bhorat, Q.E.; et al. Safety and efficacy of the ChAdOx1 nCoV-19 vaccine (AZD1222) against SARS-CoV-2: An interim analysis of four randomised controlled trials in Brazil, South Africa, and the UK. Lancet 2021, 397, 99–111.
46. Vaccine Effectiveness against Symptomatic Disease Caused by the Omicron and Delta Variants in England. News-Medical.net. 2022. Available online: https://www.news-medical.net/news/20220425/Vaccine-effectiveness-against-symptomatic-disease-caused-by-the-Omicron-and-Delta-variants-in-England.aspx (accessed on 18 August 2022).
47. Mascellino, M.T.; Di Timoteo, F.; De Angelis, M.; Oliva, A. Overview of the Main Anti-SARS-CoV-2 Vaccines: Mechanism of Action, Efficacy and Safety. Infect. Drug Resist. 2021, 14, 3459–3476.
48. Bos, R.; Rutten, L.; van der Lubbe, J.E.M.; Bakkers, M.J.G.; Hardenberg, G.; Wegmann, F.; Zuijdgeest, D.; de Wilde, A.H.; Koornneef, A.; Verwilligen, A.; et al. Ad26 vector-based COVID-19 vaccine encoding a prefusion-stabilized SARS-CoV-2 Spike immunogen induces potent humoral and cellular immune responses. NPJ Vaccines 2020, 5, 91.
49. Sputnik Light Booster after Sputnik V Vaccination Induces Robust Neutralizing Antibody Response to B.1.1.529 (Omicron) SARS-CoV-2 Variant|Medrxiv. Available online: https://www.medrxiv.org/content/10.1101/2021.12.17.21267976v1 (accessed on 16 August 2022).
50. Tukhvatulin, A.I.; Dolzhikova, I.V.; Shcheblyakov, D.V.; Zubkova, O.V.; Dzharullaeva, A.S.; Kovyrshina, A.V.; Lubenets, N.L.; Grousova, D.M.; Erokhova, A.S.; Botikov, A.G.; et al. An open, non-randomised, phase 1/2 trial on the safety, tolerability, and immunogenicity of single-dose vaccine “Sputnik Light” for prevention of coronavirus infection in healthy adults. Lancet Reg. Health Eur. 2021, 11, 100241.
51. Evidence Assessment: Sinopharm/BBIBP COVID-19 Vaccine. 2021. Available online: https://cdn.who.int/media/docs/default-source/immunization/sage/2021/april/2_sage29apr2021_critical-evidence_sinopharm.pdf (accessed on 28 October 2022).
52. Wang, C.; Chen, L.Y.; Lu, Q.B.; Cui, F. Vaccination with the Inactivated Vaccine (Sinopharm BBIBP-CorV) Ensures Protection against SARS-CoV-2 Related Disease. Vaccines 2022, 10, 920.
53. Tanriover, M.D.; Doğanay, H.L.; Akova, M.; Güner, H.R.; Azap, A.; Akhan, S.; Köse, S.; Erdinc, F.; Akalın, E.H.; Tabak, O.F.; et al. Efficacy and safety of an inactivated whole-virion SARS-CoV-2 vaccine (CoronaVac): Interim results of a double-blind, randomised, placebo-controlled, phase 3 trial in Turkey. Lancet 2021, 398, 213–222.
54. The CanSino Biologics Ad5-nCoV-S [recombinant] COVID-19 Vaccine: What You Need to Know. [cited 2022 Aug 18]. Available online: https://www.who.int/news-room/feature-stories/detail/the--cansino-biologics-ad5-ncov-s--recombinant---covid-19-vaccine--what-you-need-to-know (accessed on 18 August 2022).
55. Coronavirus Disease (COVID-19): Vaccines. Available online: https://www.who.int/news-room/questions-and-answers/item/coronavirus-disease-(covid-19)-vaccines (accessed on 31 August 2022).
56. Kozlov, V.A.; Tikhonova, E.P.; Savchenko, A.A.; Kudryavtsev, I.V.; Andronova, N.V.; Anisimova, E.N.; Golovkin, A.S.; Demina, D.V.; Zdzitovetsky, D.E.; Kalinina, Y.U.S.; et al. Borisov. Clinical Immunology. In A Practical Guide for Infectious Disease Specialists; Polikor: Krasnoyarsk, Russia, 2021; p. 563. https://doi.org/10.17513/np.438
57. Tardiolo, G.; Brianti, P.; Sapienza, D.; dell’Utri, P.; Dio, V.D.; Rao, G.; Calabrò, R.S. Are We Paving the Way to Dig Out of the “Pandemic Hole”? A Narrative Review on SARS-CoV-2 Vaccination: From Animal Models to Human Immunization. Sci. 2021, 9, 53.
58. Alu, A.; Chen, L.; Lei, H.; Wei, Y.; Tian, X.; Wei, X. Intranasal COVID-19 vaccines: From bench to bed. eBioMedicine 2022, 76, 103841.
59. Voronina, E.; Doguzova, V. The Ministry of Health Has Registered a Nasal “Sputnik”. Available online: https://pharmvestnik.ru/content/news/Minzdrav-zaregistriroval-nazalnyi-Sputnik.html (accessed on 18 July 2022).
60. Combined Vector Vaccine for the Prevention of Coronavirus Infection Caused by the SARS-CoV-2 Virus. Medi.Ru. Available online: https://medi.ru/instrukciya/salnavak_27297/ (accessed on 15 August 2022).
61. Arnold, L.M.; Emir, B.; Murphy, T.K.; Zeiher, B.G.; Pauer, L.; Scott, G.; Petersel, D. Safety profile and tolerability of up to 1 year of pregabalin treatment in 3 open-label extension studies in patients with fibromyalgia. Ther. 2012, 34, 1092–1102.
62. A Phase 2, Double-Blind, Multi-Center, Randomized, Placebo-Controlled, Dose-Ranging Trial to Determine the Safety, Immunogenicity and Efficacy of an Adenoviral-Vector Based Vaccine Expressing Severe Acute Respiratory Syndrome (SARS-CoV-2) and dsRNA Adjuvant Administered Orally. clinicaltrials.gov; Report No.: NCT05067933. 2022 May. Available online: https://clinicaltrials.gov/ct2/show/NCT05067933 (accessed on 14 August 2022).
63. Vaxart COVID-19 Oral Vaccine. Available online: https://www.precisionvaccinations.com/vaccines/vaxart-covid-19-oral-vaccine (accessed on 15 August 2022).
64. Brand M. Pipeline [Internet]. iosBio. Available online: https://iosbio.com/pipeline/ (accessed on 15 August 2022).
65. Levin, M.J.; Ustianowski, A.; De Wit, S.; Launay, O.; Avila, M.; Templeton, A.; Yuan, Y.; Seegobin, S.; Ellery, A.; Levinson, D.J.; et al. Intramuscular AZD7442 (Tixagevimab-Cilgavimab) for Prevention of Covid-19. Engl. J. Med. 2022, 386, 2188–2200.
66. Case, J.B.; Mackin, S.; Errico, J.M.; Chong, Z.; Madden, E.A.; Whitener, B.; Guarino, B.; Schmid, M.A.; Rosenthal, K.; Ren, K.; et al. Resilience of S309 and AZD7442 monoclonal antibody treatments against infection by SARS-CoV-2 Omicron lineage strains. Nat. Commun. 2022, 13, 3824.
67. Clinical Evaluation of New Vaccines. European Medicines Agency. 2018. Available online: https://www.ema.europa.eu/en/clinical-evaluation-new-vaccines (accessed on 31 August 2022).
68. Guidelines on Clinical Evaluation of Vaccines: Regulatory Expectations. Available online: https://www.who.int/publications/m/item/WHO-TRS-1004-web-annex-9 (accessed on 31 August 2022).
69. Khoury, D.S.; Cromer, D.; Reynaldi, A.; Schlub, T.E.; Wheatley, A.K.; Juno, J.A.; Subbarao, K.; Kent, S.J.; Triccas, J.A.; Davenport , M.P. Neutralizing antibody levels are highly predictive of immune protection from symptomatic SARS-CoV-2 infection. Nat. Med. 2021, 27, 1205–1211.
70. Kudlay, D.; Kofiadi, I.; Khaitov, M. Peculiarities of the T Cell Immune Response in COVID-19. Vaccines 2022, 10, 242.
71. Martynova, E.; Hamza, S.; Garanina, E.E.; Kabwe, E.; Markelova, M.; Shakirova, V.; Khaertynova, I.M.; Kaushal, N.; Baranwal, M.; Rizvanov, A.A.; et al. Long Term Immune Response Produced by the SputnikV Vaccine. J. Mol. Sci. 2021, 22, 11211.
72. Lapa, D.; Grousova, D.M.; Matusali, G.; Meschi, S.; Colavita, F.; Bettini, A.; Gramigna, G.; Francalancia, M.; Garbuglia, A.R.; Girardi, E.; et al. Retention of Neutralizing Response against SARS-CoV-2 Omicron Variant in Sputnik V-Vaccinated Individuals. Vaccines 2022, 10, 817.
73. Liu, J.; Chandrashekar, A.; Sellers, D.; Barrett, J.; Lifton, M.; McMahan, K.; Sciacca, M.; VanWyk, H.; Wu, C.; Yu, J.; et al. Vaccines Elicit Highly Cross-Reactive Cellular Immunity to the SARS-CoV-2 Omicron Variant. medRxiv [Preprint]. 2022. http://doi.org/ 10.1101/2022.01.02.22268634.
74. He, Q.; Mao, Q.; Zhang, J.; Bian, L.; Gao, F.; Wang, J.; Xu, M.; Liang, Z. COVID-19 Vaccines: Current Understanding on Immunogenicity, Safety, and Further Considerations. Immunol. 2021, 12, 669339.
75. Ashmawy, R.; Hamdy, N.A.; Elhadi, Y.A.M.; Alqutub, S.T.; Esmail, O.F.; Abdou, M.S.M.; Reyad, O.A.; El-Ganainy, S.O.; Gad, B.K.; El-Deen, A.E.-S.N.; et al. A Meta-Analysis on the Safety and Immunogenicity of Covid-19 Vaccines. Prim. Care Community Health 2022, 13, 21501319221089256.
76. Clinical Guidance for COVID-19 Vaccination|CDC. Available online: https://www.cdc.gov/vaccines/covid-19/clinical-considerations/interim-considerations-us.html (accessed on 31 August 2022).
77. Care AGD of H and A. ATAGI Clinical Guidance for COVID-19 Vaccine Providers. Australian Government Department of Health and Aged Care. Australian Government Department of Health and Aged Care. 2021. Available online: https://www.health.gov.au/initiatives-and-programs/covid-19-vaccines/advice-for-providers/clinical-guidance (accessed on 31 August 2022).
78. Temporary Guidelines for Prevention, Diagnosis and Treatment of Novel Coronavirus Disease (COVID-19) Version 16.pdf. Available online: https://static-0.minzdrav.gov.ru/system/attachments/attaches/000/060/193/original/%D0%92%D0%9C%D0%A0_COVID-19_V16.pdf (accessed on 30 August 2022).
79. pdf. Available online: https://static-0.minzdrav.gov.ru/system/attachments/attaches/000/057/333/original/05072021_MR_Preg_v4.pdf (accessed on 20 July 2022).
80. Canada PHA of. Archive 38: COVID-19 Vaccine Guidance Updates: Canadian Immunization Guide: June 21, 2022 [2022-06-21]. 2022. Available online: https://www.canada.ca/en/public-health/services/immunization/national-advisory-committee-on-immunization-naci/summary-updates-canadian-immunization-guide-june-21-2022-covid-19-vaccines.html (accessed on 31 August 2022).
81. Temporary Guidelines. The Procedure for Vaccination against a New Coronavirus Infection (COVID-19). 2022. Available online: https://static-0.minzdrav.gov.ru/system/attachments/attaches/000/060/087/original/%D0%9C%D0%B5%D1%82%D0%BE%D0%B4%D0%B8%D1%87%D0%B5%D1%81%D0%BA%D0%B8%D0%B5_%D1%80%D0%B5%D0%BA%D0%BE%D0%BC%D0%B5%D0%BD%D0%B4%D0%B0%D1%86%D0%B8%D0%B8_02062022_%282%29.pdf?1655803717 (accessed on 18 August 2022).
82. Canada PHA of. COVID-19 Vaccine: Canadian Immunization Guide. 2021. Available online: https://www.canada.ca/en/public-health/services/publications/healthy-living/canadian-immunization-guide-part-4-active-vaccines/page-26-covid-19-vaccine.html (accessed on 31 August 2022).
83. Letter of the Ministry of Health of Russia Dated December 22, 2021 N 30-4/I/2-21694 “On the Submission of Updated Temporary Guidelines. The Procedure for Vaccination against a New Coronavirus Infection (COVID-19) Available online: https://spboms.ru/sites/default/files/pismo_minzdrava_rossii_ot_22_12_2021_n_30-4_i_2-21694_o.pdf (accessed on 31 August 2022)
84. COVID-19 Vaccination: Booster Dose Resources—GOV.UK. Available online: https://www.gov.uk/government/publications/covid-19-vaccination-booster-dose-resources (accessed on 31 August 2022).
85. About Clarification of the Procedure for Re-Vaccination against Covid-19. Available online: http://www.7gsp.by/polezno-znat/stati/558-o-razyasnenii-poryadka-provedeniya-povtornoj-vaktsinatsii-protiv-covid-19 (accessed on 14 August 2022).
86. Abu-Raddad, L.J.; Chemaitelly, H.; Ayoub, H.H.; AlMukdad, S.; Yassine, H.M.; Al-Khatib, H.A.; Smatti, M.K.; Tang, P.; Hasan, M.R.; Coyle, P.; et al. Effect of mRNA Vaccine Boosters against SARS-CoV-2 Omicron Infection in Qatar. Engl. J. Med. 2022, 386, 1804–1816.
87. Coronavirus Disease 2019 (COVID-19). Centers for Disease Control and Prevention. 2020. Available online: https://www.cdc.gov/coronavirus/2019-ncov/variants/variant-classifications.html (accessed on 31 August 2022).
88. UK Grants World’s First Approval for Moderna’s Omicron Covid Vaccine. Bloomberg.com. 2022. Available online: https://www.bloomberg.com/news/articles/2022-08-15/uk-takes-world-s-first-step-toward-omicron-tailored-covid-shot (accessed on 25 August 2022).
89. Au, W.Y.; Cheung, P.P.H. Effectiveness of heterologous and homologous covid-19 vaccine regimens: Living systematic review with network meta-analysis. BMJ 2022, 377, e069989.
90. Tracking SARS-CoV-2 Variants. Available online: https://www.who.int/activities/tracking-SARS-CoV-2-variants (accessed on 31 August 2022).
91. Mohammadi, M.; Shayestehpour, M.; Mirzaei, H. The impact of spike mutated variants of SARS-CoV2 [Alpha, Beta, Gamma, Delta, and Lambda] on the efficacy of subunit recombinant vaccines. J. Infect. Dis. 2021, 25, 101606.
92. Wang, P.; Nair, M.S.; Liu, L.; Iketani, S.; Luo, Y.; Guo, Y.; et al. Antibody Resistance of SARS-CoV-2 Variants, B.1.351 and B.1.1.7. Nature 2021, 593, 130–135.
93. Singh, M.; Novitsky, V.; Carpenter-Azevedo, K.; Howison, M.; Huard, R.C.; King, E.; Kantor, R. SARS-CoV-2 Variants in Rhode Island; May 2022 Update. RI Med. J. 2022, 105, 6–11
94. Parums, D.V. Editorial: World Health Organization (WHO) Variants of Concern Lineages Under Monitoring (VOC-LUM) in Response to the Global Spread of Lineages and Sublineages of Omicron, or B.1.1.529, SARS-CoV-2. Sci. Monit. Int. Med. J. Exp. Clin. Res. 2022, 28, e937676.
95. Statement on Omicron Sublineage BA.2. Available online: https://www.who.int/ru/news/item/22-02-2022-statement-on-omicron-sublineage-ba.2 (accessed on 15 August 2022).
96. WHO-2019-nCoV-Vaccine-Effectiveness-Measurement-2021.1-Eng.pdf. Available online: https://apps.who.int/iris/bitstream/handle/10665/340301/WHO-2019-nCoV-vaccine-effectiveness-measurement-2021.1-eng.pdf?sequence=1&isAllowed=y (accessed on 31 August 2022).
97. Noor, R.; Shareen, S.; Billah, M. COVID-19 vaccines: Their effectiveness against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its emerging variants. Natl. Res. Cent. 2022, 46, 96.
98. Bhatnagar, T.; Chaudhuri, S.; Ponnaiah, M.; Yadav, P.D.; Sabarinathan, R.; Sahay, R.R.; Ahmed, F.; Aswathy, S.; Bhardwaj, P.; Bilimale, A.; et al. Effectiveness of BBV152/Covaxin and AZD1222/Covishield vaccines against severe COVID-19 and B.1.617.2/Delta variant in India, 2021: A multi-centric hospital-based case-control study. J. Infect. Dis. 2022, 122, 693–702.
99. Ikegame, S.; Siddiquey, M.N.A.; Hung, C.T.; Haas, G.; Brambilla, L.; Oguntuyo, K.Y.; Kowdle, S.; Chiu, H.-P.; Stevens, C.S.; Vilardo, A.E.; et al. Neutralizing activity of Sputnik V vaccine sera against SARS-CoV-2 variants. Commun. 2021, 12, 4598.
100. Marta, R.A.; Nakamura, G.E.K.; de Matos Aquino, B.; Bignardi, P.R. COVID-19 Vaccines: Update of the vaccines in use and under development. Vacunas 2022, 23, S88–S102.
101. Zhou, Z.; Zhu, Y.; Chu, M. Role of COVID-19 Vaccines in SARS-CoV-2 Variants. Immunol. 2022, 13, 898192.
102. Grewal, R.; Nguyen, L.; Buchan, S.A.; Wilson, S.E.; Nasreen, S.; Austin, P.C.; Brown, K.A.; Fell, D.B.; Gubbay, J.; Schwartz, K.L. et al. NACI Recommendations on the use of bivalent Omicron-containing mRNA COVID-19 vaccines. medRxiv 2022.
103. Moderna: Spikevax Bivalent Original/Omicron BA.4/BA.5—COVID19 Vaccine Tracker. Available online: https://covid19.trackvaccines.org/vaccines/224/ (accessed on 29 October 2022).
104. Adapted Vaccine Targeting BA.4 and BA.5 Omicron Variants Original SARS-CoV-2 Recommended for Approval. European Medicines Agency. 2022. Available online: https://www.ema.europa.eu/en/news/adapted-vaccine-targeting-ba4-ba5-omicron-variants-original-sars-cov-2-recommended-approval (accessed on 28 October 2022).
105. EMA Starts Rolling Review of COVID-19 Vaccine (Vero Cell) Inactivated. European Medicines Agency. 2021. Available online: https://www.ema.europa.eu/en/news/ema-starts-rolling-review-covid-19-vaccine-vero-cell-inactivated (accessed on 14 August 2022).
106. Order of the Ministry of Health of the Russian Federation Dated December 6, 2021 N 1122n On the Approval of the National Calendar of Preventive Vaccinations, the Calendar of Preventive Vaccinations for Epidemic Indications and the Procedure for Conducting Preventive Vaccinations. Available online: https://docs.cntd.ru/document/727605537?ysclid=l7gh4jwwkz698458785 (accessed on 30 August 2022).

This entry is adapted from the peer-reviewed paper 10.3390/bioengineering9110714