Prebiotic Potential of Dietary Beans and Pulses: History
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Subjects: Endocrinology & Metabolism | Microbiology | Food Science & Technology
Contributor:
Ravinder Nagpal
,
Saurabh Kadyan
,
,
Bahram Arjmandi
,
Prashant Singh

Dietary pulses, including dry beans, lentils, chickpeas, and dry peas, have the highest proportion of fiber among different legume cultivars and are inexpensive, easily accessible, and have a long shelf-life. The inclusion of pulses in regular dietary patterns is an easy and effective solution for achieving recommended fiber intake and maintaining a healthier gut and overall health. Dietary pulses-derived resistant starch (RS) is a relatively less explored prebiotic ingredient. Several in vitro and preclinical studies have elucidated the crucial role of RS in fostering and shaping the gut microbiota composition towards homeostasis thereby improving host metabolic health. However, in humans and aged animal models, the effect of only the cereals and tubers derived RS has been studied.

  • aging
  • beans
  • fiber
  • gut health
  • lentils
  • microbiota
  • microbiome
  • prebiotic
  • pulses
  • resistant starch

1. Introduction

Pulses are valuable dry grains from leguminous crops. Domesticated around 10,000 years ago, pulses have been consumed as a key staple food crop, especially in developing nations, thus providing a primary means of protein and energy [1]. However, the past century has witnessed a change in the eating habits of the population, especially the decline of the pulse consumption in the daily diet and a surge in the chronic disease rates [2]. Based on a posteriori and a priori dietary patterns, consumption of whole grains and legumes/pulses are linked with longevity and better cardiovascular, metabolic, and cognitive health [3]. On the contrary, diets rich in refined grains, red meat, and sugar have been associated with an increased risk of mortality and adverse cardiometabolic outcomes [3].
Although there are numerous pulse varieties available worldwide, Food and Agriculture Organization (FAO) has listed 11 main types, namely beans, broad beans, Bambara beans, chickpeas, lentils, cowpeas, peas, pigeon peas, vetches, lupins, and other “minor” pulses [4]. Among them, lentils (Lens culinaris L.), beans (Paseolus vulgaris L.), chickpeas (Cicer arietinum L.), and peas (Pisum sativum L.) are the most frequently consumed pulses worldwide [5]. Pulses possess superior nutritional properties and harbor various bioactive compounds, viz., fermentable fibers, bioactive peptides, and phytochemicals [6]. The high nutritional value of pulses is attributed to their high-quality protein and soluble and insoluble dietary fiber [7]. The daily intake of dietary fiber at a level of 14 g/1000 kcal or above has been proposed to confer health benefits in human cohorts [8]. Still, a developed nation like the United States is far from achieving this level, and the magnitude of the gap is nearly 50–70% shortfall [9]. To address this shortfall, supplementation of diets with pulses could be one promising strategy as the total fiber content in pulses can range up to 30 g/100 g dry weight (peas: 14–26 g; lentils: 18–20 g; chickpeas: 18–22 g; beans: 23–32 g), with insoluble fiber being the major sub-component (peas: 10–15 g; lentils: 11–17 g; chick-peas: 10–18 g; beans: 20–28 g) [4].
Starch is the major carbohydrate in pulses accounting for nearly 50% portion of carbohydrates [10]. Certain starches present in the raw and/or cooked pulses exist in the form of dietary fiber instead of available carbohydrates. This is due to the partial or complete modification in the starch structure during heat processing of foods leading to the formation of resistant starch (RS). RS remains un-digested in the upper-gastrointestinal tract and reaches the large intestine, where it is metabolized by intestinal microbes into a wide range of metabolites, which helps in the maintenance of optimal human health [11]. Past studies have also proven the prebiotic potential of RS in improving the post-prandial glycemic and insulinemic responses, increasing satiety, reducing cholesterol and stored fat, and promoting weight loss, making it an apt ingredient, especially for the management of gut-associated metabolic disorders [12][13][14][15]. Hitherto, studies assessing the human health benefits of RS were confined to RS derived from cereals and tubers, with little to no focus given on RS derived from pulses. Recently, efforts were made in our lab to isolate and purify starches from 18 pulses which were evaluated for their functional properties in order to promote their use as superior food ingredients in industry [16]. Owing to the superior sensory property of selected pulse RS compared to traditional fibers like whole cereals, fruit fibers, etc., the supplementation of this functional ingredient in diet could act as a beneficial nutritional intervention for the control of metabolic diseases [17].
Nowadays, it has been widely popularized that the human gastrointestinal (GI) tract is a frontline mediator system wherein the intestinal bacteria aid in the digestion of dietary constituents of consumed foods and synthesizes low molecular weight bioactive molecules, which ultimately exerts a crucial role on human health and well-being [18]. The human gastrointestinal tract contains nearly 1014 microorganisms belonging to over 1000 species and has a bacterial genomic content of approximately 100 times over compared to the human genome [19]. About 95% of the total microbes present in the human body are colonized in the GI tract. The GI tract is the home of bacteria, eukaryotes, and archaea and is collectively known as gut microbiota [20]. Several factors such as the morphology of the gut, nutrient availability, pH, and presence or absence of oxygen are responsible for the variation in gut microbiota composition and growth of certain microbial taxa specific to different regions of the gut. The most common gut bacteria are associated with the four major phyla, with the most abundant being Firmicutes (65%), followed by Bacteroidetes (25%), Proteobacteria (8%), and Actinobacteria (5%). Moving down the taxonomic hierarchy, the GI tract harbors three main groups of extremophile anaerobes Clostridium coccoides group (or Clostridium cluster XIVa), Clostridium leptum group (or Clostridium cluster IV), and Bacteroides [21]. The gut microbes, together with their metabolites produced as a result of the degradation of different substrates, provide a range of immune, metabolic and neurobehavioral functions to host health.
Gut microbiota is dynamic in nature and changes continuously during the lifespan of an individual [22]. During the aging process of an individual, dynamic changes occur in behavioral, environmental, biological, and social processes. Genomic instability, epigenetic alterations, and telomere attrition are primary indicators of aging, resulting in cellular senescence, problems in nutrient sensing, and mitochondrial-related dysfunctions, which further negatively impact intercellular communication and exhaustion of stem cells [23]. Thus, the aging-associated decline in the cellular functions and immune system responses leads to chronic low-grade inflammation and increased gut permeability, thereby marking the onset of various gastrointestinal disorders, cardiometabolic disease, muscle frailty, cognitive decline, and gut dysbiosis [24]. Aging-associated problems are further aggravated by the ill effects of western diets rich in fat and sugars, which may increase the propensity towards gut dysbiosis [25]. The maintenance of a healthy and diverse gut microbiota that coevolves with our lifespan is a principal factor in the amelioration of various age-related diseases. Earlier studies by our group indicated that the severity of gut dysbiosis is higher in older cohorts than the young ones [26].

2. Resistant Starch and Human Health

Starch is a dietary carbohydrate that is commonly found in everyday food. It is the second most abundant chemical compound in the plants after cellulose. Chemically, starch is composed of two monosaccharide molecules that are amylose (linear chain) and amylopectin (branched chain). These molecules are linked together with alpha 1-4 and/or alpha 1-6 glycosidic bonds. Based on physical and physiological properties, starch can be classified into three categories, namely rapidly digestible starch, slowly digestible starch, and resistant starch (RS) [27]. Englyst and coworkers (1982), in an in vitro study, found that some portion of the starch remained undigested even after enzymatic treatment. Further studies confirmed that these starches were undigested by the amylases in the small intestine and enter the colon, where it is utilized by gut microbial communities. They named this starch fragment “resistant starch” [28]. The digestibility of the starch in the small intestine is primarily affected by the structure of the starch molecule and the ratio of amylose to amylopectin. Chemically, RS has a relatively low molecular weight (12 KDa) and has a linear structure made up of α-1,4-D-glucan moieties obtained from the retrograded amylose fraction [17].
Resistant starch is further subdivided into five types depending upon its structural features. RS type 1 (RS1) is physically inaccessible starch and has the most complex structures as it is frequently found entrapped within protein matrix or non-starch components of the plant cell wall (e.g., whole grains or pulses) [11]. Compared to RS1, the cellular structure is absent in RS type 2 (RS2). The RS type 2 possesses native, uncooked, and semi-crystalline starch granules having a B- or C-type polymorph (e.g., high-amylose starch, raw potato starch) [11]. The RS type 3 (RS3) is obtained by retrogradation process upon cooking and cooling of starch-containing foods. Its resistance to digestion could be due to lower activity of pancreatic α-amylases toward starch double helices as against fully gelatinized starch molecules (e.g., retrograded high amylose maize starch) [29]. The RS type 4 (RS4) is the starch-modified through chemical processes such as esterification, crosslinking, hydroxypropylation, acetylation, and phosphorylation [30]. The functional groups block the site of action of starch digestive enzymes, which confers resistance of RS4 to digestion. The RS type 5 (RS5) is defined as the starch obtained by complex formation between high amylose starch with the lipids, which further increases the enzyme resistance of high amylose by preventing granule swelling during cooking [17].
Resistant starch possesses many desirable functional and health-promoting properties [27]. An overview of the effect of resistant starch derived from the pulses on the health outcome of humans and rodents is summarized in Figure 1. RS fermentation in the lower GI tract produces different starch oligomers and SCFAs. SCFAs are actively involved in reducing the risk of diabetes, cancer, obesity, and other cardiovascular diseases [8][9][25]. Among them, acetate, propionate, and butyrate have been extensively studied for their health benefits. Acetate is the major SCFA that is produced to the tune of 65% in the colon resulting in significant drops in pH. Thus, it helps in the inhibition of various pathogenic microorganisms and indirectly aids in the absorption of minerals such as calcium, iron, and sodium Butyrate, on the other hand, provides energy to colonocytes, possesses anti-inflammatory properties, protects against colon cancer, and plays a key role in gut homeostasis as well as maintaining the integrity of epithelium [31]. Butyrate is also responsible for lower levels of glycolysis and glycogenolysis (Ashwar et al., 2017). Propionate is another important metabolite that is partially absorbed via portal veins and reaches the liver. It is then metabolized as a glucogenic substrate resulting in inhibition of pathways leading to reduced 3-hydroxy-3-methylglutaryl co-enzyme A (HMG-CoA) activity and suppression of acetyl-CoA reductase, thereby imparting reduction in blood plasma cholesterol levels [32]. The serum cholesterol-lowering effect of RS was demonstrated in rats when they were fed a cholesterol-free diet [33].
Nutrients 14 01726 g001 550
Figure 1. Illustration depicting the effects of resistant starches (RS) derived from dietary beans and pulses on host (rodent and human) health. ↑: increased; ↓: decreased.

3. Benefits of Dietary Beans and Pulses on Gut Health

The recent advances linking the role of dietary fibers in ameliorating different disease states have led to increased interest in pulse-based foods. Various types of fibers present in pulses include long-chain soluble and insoluble polysaccharides, resistant starch, and galactooligosaccharides. In addition, these components can act as prebiotic precursors, which are digested by beneficial microorganisms in the gut. The consumption of pulses in the diet has been linked to the reduction in serum cholesterol, increased satiety, and low post-prandial blood glucose levels, thus mitigating the risk of different metabolic diseases like cardiovascular diseases, obesity, diabetes, etc. [34][35]. In fact, several meta-analyses concluded that daily pulse intake of approximately 2/3 cups could significantly lower total and LDL cholesterol [36]. The low glycemic response of pulse is associated to the physical barrier between the starch and digestive enzymes by the intact cell wall of whole pulses after cooking. Furthermore, pulse consumption is closely associated with reducing blood pressure and providing protection against reactive oxygen species due to the presence of high levels of polyphenols [37].
In the last few years, more research has been directed towards pulses which could be a sustainable source of plant protein compared to animal protein to feed the growing population and to simultaneously address the food insecurity problems [4]. Additionally, whole pulses being rich in plant-based protein and dietary fibers underpins the hypothesis of their positive effects on the gut microbiota. Table 1 summarizes the influence of consumption of pulses in various forms—cooked, flour, meals, or supplemented in the diet, on the gut microbiota changes in rodents and humans. A study on pulse flour exhibited improved growth of genera Bifidobacterium, Faecalibacterium, Clostridium, Eubacterium, and Roseburia along with enhanced butyrate and acetate production [38]. Several studies have reported that the incorporation of pulses in the diet increases the abundance of Prevotella, Dorea, and Ruminococcus flavefaciens, and decreased abundance of Ruminococcus gnavus in mice models [18][39][40][41][42]. Prevotella is a genus possessing a large spectrum of glycoside hydrolases and is known for its ability to produce SCFAs following the carbohydrates fermentation [39]. The species Ruminococcus flavefaciens had been found to decrease in overweight (BMI: 25.0–29.9) and obese (BMI: >30.0) subjects [43]. The abundance of Ruminococcus gnavus, a mucolytic species, has been linked to an increase in gut-barrier pathologies in subjects with obesity and inflammatory bowel disease [41]. Another positive effect of pulse intake is the increased prevalence of Akkermansia muciniphila in the gut, which is often categorized as next-generation probiotics [8][44]. Interestingly, this bacterium is also mucolytic but has an inverse correlation with R. gnavus [45]. Majority of the studies reported herein demonstrated a decrease in the ratio of Firmicutes to Bacteroidetes. This reduction in the ratio of two major phyla has been associated with the amelioration of obesity, possibly due to altered energy extraction from carbohydrates metabolism in the colon [46]. Among the Bacteroidales, the members representative of the pulse-based diets includes Muribaculaceae (S24-7), Rikenellaceae and B. acidifaciens [18]. Lentil consumption was found to be associated with increased prevalence of Roseburia in mouse feces [40]. Roseburia is involved in butyrate production and has negative correlation with several diseases such as colitis and Crohn’s disease [47]. Although these studies revealed beneficial effects of pulses in positively modulating the gut microbiome, the impact on different gut genera is complex, which may be dependent upon many variables, such as pulse type, dose, age, status of cohorts, duration of the study and the sequencing methodology adopted.
Table 1. Effect of dietary pulses on gut microbiota-related changes in rodents and humans.
Pulse-Type Cohort State of Cohort Age Dose Duration of Study Key Shifts in Gut Microbiota Outcome References
Cooked chickpeas Human Healthy 18–65 years 200 g/d 3 weeks
  • Phylum
    ↑ Bacteroidetes
  • Genus
    ↑ Megasphaera
    ↓ Clostridium I, II, IV, XI clusters
  • Species
    ↑ Faecalibacterium prausnitzii
    ↓ Subdoligranulum
    ↓ Clostridium histolyticum, Clostridium lituseburense groups
  • Reduction in pathogenic and putrefactive gut bacteria species in cohorts
  • Less intestinal colonization by ammonia-producing bacterial species
 [48]
Cooked pinto beans Human Healthy; Pre-metabolic syndrome 18–51 years 130 g/d 12 weeks + 4 weeks run-in
  • Species
    ↑ Peptostreptococcus productus
    ↓ Eubacterium limosum
  • High propionate production
  • Lower serum total cholesterol, LDL, and HDL
 [49]
Cooked navy bean powder Human Colorectal cancer survivors (overweight and obese) 47–81 years 35 g/d 28 days
  • Species
    ↑ Clostridium sp.,
    ↑ Lachnospira sp.,
    ↑ Coprococcus sp.
    ↓ Bacteroides fragilis
    ↓ Anaerostipe sp.
  • Boost in microbial richness compared baseline for colorectal cancer survivors but had no effect on their diversity
 [50]
Cooked navy beans (incorporated in meals and snacks) Human Colorectal cancer survivors (overweight and obese) NB: 60.9 ± 11.0 years
Control: 65.50 ± 3.07 years		 35 g/d 4 weeks  
  • Thirty and twenty-six significant metabolite differences in stool samples from baseline and control, respectively
  • Navy bean-derived metabolites (247/560) including N-methylpipecolate, 2-aminoadipate, piperidine, and vanillate
  • Abundance of ophthalmate increased by 5.25 fold
 [51]
Beans, chickpeas, peas, or lentils-based foods Human Healthy 57 ± 6.3 150 g/d 4 months  
  • Reduction in total cholesterol and LDC by 8.3% and 7.9%
 [52]
Dolichos lablab L. (standardized extract) Mice
(C57BL/6 male)		 IBS model 7 weeks 100–400 mg/kg 15 days  
  • Minimized weight loss with no effect on food intake
  • Attenuated zymosan-induced colonic macroscopic scores
  • Reduced mast cell count, TNF-α in the colon
  • Reduced visceral pain-related behaviors
  • Dose-dependent reduction of c-Fos expression in the brain
 [53]
Chickpea supplemented diet Mice (C57BL/6 male) Healthy 5 weeks 200 g/kg diet 3 weeks
  • Family
    ↓ Clostridiaceae
    (feces only)
    ↓ Peptococcaceae
  • Genus
    ↑ Prevotella
    ↑ Dorea
  • Species
    ↑ Ruminococcus flavefaciens
    ↓ Bifidobacterium pseudolongum
    ↓ Parabacteroides distansonis
    ↓ Undefined sp. in the Ruminococcus genus (cecal only)
    ↓ Lactococcus
    ↓ Turicibacter
  • Enhanced colon crypt mucus content and mucin mRNA expression
  • Improved expression of epithelial tight junction proteins
  • Enhanced metagenomic functions (e.g., ↑ butanoate metabolism; ↑ flavonoid biosynthesis)
  • Increased SCFAs production
  • Enhanced taxa richness in the cecum
 [41]
Cooked white and dark red kidney beans Mice (C57BL/6 male) DSS induced colitis 5 weeks BD + 20% beans 3 weeks  
  • Enhanced acetate, butyrate, and propionate production
  • Increased colon crypt height, and MUC1 and Relmβ mRNA expression
  • Reduced serum levels of IL-17A, TNF-α, IFN-γ, IL-1β, and IL-6
 [42]
Cooked Navy bean or black bean Mice
(C57Bl/6
male)		 Healthy 4 weeks Supplementation @20% to the basal diet 3 weeks
  • Genus
    ↑ Prevotella
    ↑ S24-7
    ↑ undefined genera within the Clostridiales order and Coriobacteriaceae family (BB only)
    ↓ Oscillospira,
    ↓ Ruminococcus
    ↓ Coprococcus
    ↓ Lactococcus,
    ↓ Streptococcus
    ↓ rc4-4
    ↓ Coprobacillus
    ↓ Parabacteroides
    ↓ Aldercreutzia
    ↓ unassigned members o Peptococcaceae, Erysipelotrichaceae, Clostridiaceae, Mogibacteriaeae, Peptostreptococcaceae, Christensenellaceae, and Rikenellaceae families
  • Species
    ↑ Ruminococcus flavefaciens
    ↓ Ruminococcus gnavus
    ↓ Clostridium perfringens (NB only)
    ↓ undefined species in the Lachnospiraceae family (BB only)
  • Enhanced SCFAs production and expression of receptors GPR-41, 43, 109
  • Increased crypt length, epithelial cell proliferation, goblet cell number, crypt mucus level, and mucin mRNA expression
  • Reduced serum endotoxin concentration
  • Enhanced apical junctional complex components (occludin, JAM-A, ZO-1, and E-cadherin)
 [39]
Cranberry beans Mice
(C57BL/6 male)		 Healthy and DSS induced colitis 5 weeks BD + 20% beans 3 weeks
  • Family
    ↑ Prevotellaceae
    ↓ Lactobacillaceae
    ↓ Clostridiaceae
    ↓ Peptococcaceae
    ↓ Peptostreptococcaceae
    ↓ Rikenellaceae
    ↓ Pophyromonadacea
  • Genus
    ↑ S24-7
  • Species
    ↓ Ruminococcus gnavus
    ↓ Clostridium perfringens
 In healthy cohorts:
  • Increased cecal SCFAs, colon crypt height, crypt goblet cell number, and mucus content
  • Enhanced expression of Muc1, Klf4, Relmβ, and Reg3γ


In diseased cohorts:
  • Reduced disease severity and colonic histological damage
  • Increased gene expression of barrier function genes (Relmβ, Muc1-3, and Reg3γ)
  • Diminishing of colonic and circulating inflammatory cytokines (IL-1β, IFNγ, IL-6, and TNF-α)
 [54]
Lentil, chickpea, bean, and dry pea Mice
(C57BL/6NCrl mice)		 Healthy 3–4 weeks 40 g/100 g obesogenic diet (by replacing 35% protein) 17 weeks
  • Phylum
    ↑ Bacteroidetes (highest in lentil)
    ↑ Verrucomicrobia (in bean and lentil)
    ↓ Firmicutes
    ↓ Proteobacteria
  • Family
    ↑ Muribaculaceae
    ↑ Rikenellaceae
    ↑ Mogibacteriaceae
    ↓ Peptococcaceae
    ↓ Christensenellaceae
  • Genus
    ↑ Allobaculum
    ↑ Sutterella (II) ↑ rc4 4 (of Peptococcaceae),
    ↑ RF32 (of Alphaproteobacteria)
    ↓ Oscillospira
    ↓ Dorea
    ↓ Lactococcus
    ↓ Streptococcus
  • Species
    ↑ B. acidifaciens
    ↑ B. pullicaecorum
    ↓ R. gnavus
    ↓ M. schaedleri
    ↓ C. methylpentosum
  • High a-diversity, especially for chickpea and dry pea
  • High b-diversity
  • Altered gut microbiota suggestive of anti-obesogenic physiologic outcomes
 [18]
Cooked red lentils Mice
(C57Bl/6 male)		 Healthy 5 weeks 20% w/w basal diet 3 weeks
  • Phylum
    ↑ Firmicutes
    ↓ Bacteroidetes
  • Family
    ↓ Parabacteroides
  • Genus
    ↑ Coprococcus
    ↑ Dorea
    ↑ Roseburia
    ↑ Turicibacter
    ↑ Prevotella
    ↑ Unknown genus belonging to the Lachnospiraceae family
  • Improved fecal microbiota α-diversity
  • Abundance of SCFA producing bacteria
  • Increased mRNA expression of SCFA receptors (GPR 41,43), tight junction proteins (E-cadherin, Zona Occulden-1 Claudin-2)
 [40]
Chickpea, lentil, dry peas, and bean Mice
(C57BL/6 male)		 Obese 3–4 weeks 40% w/w diet 17 weeks
  • Phylum
    ↑ Bacteroidetes
    ↓ Firmicutes (statistically significant in bean and lentil diet)
  • Species
    ↑ Akkermansia muciniphila (bean and lentil fed diet only)
  • Three fold elevation of bacterial count in the cecum
  • 2.2–5 fold increase in Bacteroidetes to Firmicutes ratio
  • Reduced lipid accumulation in adipose tissue
  • Decreased subcutaneous and visceral fat mass compared to high-fat control but greater compared to a low-fat control
  • 108 differential metabolites identified related to pulse types
 [8]
Whole mung bean Mice
(C57BL/6 male)		 Diet-induced obesity
(1 w HFD feeding)		 4 weeks HFD + 30% bean 12 weeks
  • Phylum
    ↑ Bacteroidetes
    ↓ Firmicutes
  • Family
    ↑ Lachnospiraceae
    ↑ Ruminococcaceae
    ↓ unassigned member of Lachnospiraceae
  • Genus
    ↑ Blautia
    ↑ unassigned member of Muribaculaceae
    ↑ Turicibacter
    ↑ Akkermansia
    ↑ Bacteroides
    ↑ Bifidobacterium
    ↓ Ruminiclostridium
    ↓ Mucispirillum
    ↓ Ruminiclostridium
    ↓ unassigned member of Ruminococcaceae
    ↓ Oscillibacter
  • Reduction in hepatic steatosis
  • Reduction in body weight gain, fat accumulation, and adipocyte size
  • Significant a- and b- diversity
  • Ameliorated insulin resistance and glucose tolerance
  • Normalization of HFD-induced gut microbiota dysbiosis
 [44]
Lentil (Lens culinaris Medikus) Rats
(Sprague−Dawley)		 Healthy 8 weeks 70.8% red lentil diet 6 weeks
  • Phylum
    ↑ Actinobacteria
    ↑ Bacteroidetes
    ↓ Firmicutes
  • Family
    ↓ Lachnospiraceae
    ↓ Streptococcaceae
  • Species
    ↑ Shutterworthia satelle
  • Reduced mean body weight
  • Reduction in body fat and blood plasma triglycerol levels
 [55]
Yellow pea flour Rats Diet-induced obesity (5 w HFD feeding) 5 weeks 30% w/w diet 42 days
  • Phylum
    ↓ Firmicutes
  • Species
    ↓ C. leptum (cluster IV)
  • Attenuated weight gain
  • Low body fat
 [46]
Whole yellow pea flour Hamster (Golden Syrian) Hypercholesterolemic diet (28 days) 2 weeks 10% replacement of corn starch with pea flour in the diet 28 days
  • Order
    ↑ Lactobacillales
  • Genus
    ↑ Unclassified clostridia
    ↑ Bacilli
  • Reduced insulin levels
  • High energy expenditure
 [56]
NB: navy bean; BB: black bean; DSS: dextran sodium sulphate; IBS: irritable bowel syndrome; BD: basal diet; ↑: increased; ↓: decreased.
Common beans, chickpea, and lentils have been shown to exert positive effects in the modulation of the colonic microenvironment in animal models [18][39][40][42]. These include enhancement of (i) crypt mucus content and mucin mRNA expression; (ii) expression of epithelial tight junction proteins; (iii) crypt length, epithelial cell proliferation, and goblet cell number; (iv) SCFAs levels (acetate, propionate, and butyrate); (v) expression of G protein-coupled receptors in the intestine; (vi) reduced pro-inflammatory cytokines in the serum. Increased expression of G protein-coupled receptors in the colon is related to sensing high SCFA production by gut microbes which are implicated in adipose tissue metabolism and appetite regulation [57]. The benign role of whole pulse consumption in the modulation of human gut microbiota and metabolite profile have also been explored in the past by researchers using clinical trials [48][49][50][51][52]. Some of these include reduction in pathogenic and putrefactive gut bacteria species; increase in Bacteroidetes and Faecalibacterium prausnitzii; decreased total serum cholesterol, LDL- and HDL-cholesterol; boost in microbial richness, and significant change in metabolite profile (e.g., ophthalmate) in colorectal cancer survivors.

4. Prebiotic Potential of Pulses-Derived Resistant Starch for Gut Health

The concentration of SCFAs in the lower GI tract normally reduces from the proximal to the distal colon. The amount of SCFAs production is majorly dependent upon the amount of fiber reaching the distal colon. Therefore, one way of increasing the SCFAs in the distal gut is the selection of dietary fibers, which are minimally digested prior to reaching the distal colon. Increasing the consumption of resistant starches in the diet is a promising strategy to modulate gut health and benefit the host.
Native RS is present in varying proportions in cereals, tubers, and legumes. In addition, the RS content can be altered using cooking and cooling operations. Interestingly, the comparison of RS content among cooked cereals, legumes, and tubers samples showed legumes with the highest RS content [58]. Brummer, Kaviani and Tosh [6] reported that cooked pulses have a relatively high proportion of resistant starch (3.75–4.66% of pulse dry weight basis) than many other cooked foods. Similarly, Garcia-Alonso et al. [59] reported a marginal increase in the RS content of chickpeas, lentils, and common beans upon boiling, cooling, and reheating. Retrogradation of the gelatinized starch post-cooking and cooling is usually associated with the increased content of resistant starch in the cooked pulses [60]. Still, the amount of RS in raw, baked, and boiled pulses differ significantly, and it is a function of its intrinsic factors (e.g., amylose to amylopectin ratio, crystallinity, granular structure) and external factors (e.g., processing methods employed, storage period and conditions [13]. In brown lentils (Lens culinaris, Medikus), RS content was further increased by the addition of lipids, resulting in the formation of amylose-lipid complexes (RS5 type) [61].

This entry is adapted from the peer-reviewed paper 10.3390/nu14091726

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