1. ^{[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47]}Introduction

Locusts are short-horned grasshoppers (Orthoptera) belonging to the family Acrididae. A hallmark of locust biology is their density-dependent phase-plasticity, in which individuals will express vastly different behavioral, morphological, and physiological phenotypes according to the different population density levels they experience [1–3]. Locusts are probably best known, however, for their devastating potential to cause immense damage to natural vegetation and crops, as they are able to aggregate and migrate in swarms so large and destructive that they are commonly termed a plague. Consequently, locusts have been the focus of extensive research efforts, mostly seeking to better understand the mechanisms underlying the locust swarming phenomenon.

Although not part of the major emphasis of previous locust research, data concerning the characterization of locusts’ bacterial symbionts were published already in the late 1950s [4–6]; and since then a multitude of important observations have accumulated regarding the nature of locust–bacteria interactions [7–18]. Some of these findings were already summarized, nearly 20 years ago, by Dillon and Charnley [10], but ever since their extensive review, many new data have been collected, and vast improvements in sequencing technology have allowed researchers to ask and answer questions previously beyond our reach. The wealth of new findings in recent years calls for an update, incorporating the essential details of our current knowledge of locust-bacteria associations.

In this concise review, we provide an updated summary of the nature of locust bacterial symbionts and their distribution in the host, as well as hypothesized effects on the locust biology and life history. We mainly focus on the desert locust (Schistocerca gregaria) and the migratory locust (Locusta migratoria), which most of the studies conducted to date have explored. However, the main ideas presented will be largely applicable to other locust species, albeit perhaps with different bacterial players.

2. Locust Bacterial Symbionts and Their Location

Similar to most vertebrates and invertebrates, locusts harbor bacterial cells both within their internal body environment and externally on their cuticle [19,20]. Below we briefly present major data that have been gathered concerning the bacterial symbionts present in the locust digestive tract, as well as some more recent reports describing the bacterial symbionts in other parts of the locust’s body.

2.1. The Digestive Tract

The bacterial composition of the locust gut has been thoroughly described for the desert locust [7,10–12,15,18,21]. Some research has also been conducted on several other locust species, including the migratory locust [14,22,23], the brown locust (Locustana pardalina), the Moroccan locust (Dociostaurus maroccanus), and the Italian locust (Calliptamus italicus) [13].

The composition of the bacterial community in any insect alimentary tract is largely affected by its specific anatomy. The locust gut is relatively simple and consists of three main parts: foregut, midgut, and hindgut (Figure 1a). In brief, the cuticle-lined foregut is where the ingested food is mixed with salivary and regurgitated enzymes to initiate the food breakdown process; the food is then transferred to the midgut, where the digestion is completed and nutrients (mainly protein and carbohydrates derivatives, i.e., amino acids and monosaccharides) are absorbed, while a peritrophic membrane envelops the digested food. Six caeca invaginations are connected to the anterior part of the midgut, each opens individually into the alimentary canal (Figure 1a). The main role of the hindgut is the absorption of water, salts, amino acids, and small metabolites. Six large lobes, called “rectal pads”, maximize the surface area of the hindgut to enable efficient absorption [1,24,25].

Hunt and Charnley [8] used microscopy and cultivation-dependent methods to identify the bacterial residents in different parts of the S. gregaria alimentary canal. They observed that the bacterial load was small in the anterior parts of the digestive system, and that this load increased significantly in the posterior parts. They further reported that no bacterial cells were found on the lining of the foregut or on the epithelial cells of the midgut (including caeca), where bacteria were limited to the lumen and to the inner section of the peritrophic membrane. Furthermore, they noted that the bacteria found in the foregut were roughly similar to those found in the locust’s food, while the hindgut contained a high load of Enterobacteriaceae and Streptococcaceae. These important findings served to focus subsequent studies on those bacteria residing in the locust hindgut.

Figure 1. The main bacterial families known to inhabit different body parts of the desert locust. (a) A depiction of the locust’s alimentary canal and the common bacterial families residing within the hindgut. Foregut and hindgut (including caeca) do not host a constant fraction of specific bacterial strains. [11,15]. (b) A depiction of the female locust reproductive system divided into three main sections, all harboring the same core bacterial families [16]. (c) External bacteria found on the head and abdomen cuticle of the desert locust [18,26].

Several reports on the hindgut bacteria of S. gregaria have revealed a relatively simple bacterial composition, with a consistent presence of members of the families Enterobacteriaceae (mostly the genera Enterobacter and Klebsiella) and Enterocococeaea reviewed in [10] and described in [15,16,18,21,26]. Enterobacteriaceae and Enterocococeae members were also found in the hindgut of healthy L. migratoria [14], as well as in the gut of field-caught Italian locusts, Moroccan locusts and brown locusts [13].

Based on the fact that desert locusts were shown to be able to live and reproduce without their gut bacterial symbionts, at least under laboratory conditions [27], it is accepted that locusts have developed a tight but not obligatory association with these mutualistic bacteria. Yet, some bacteria seem to play an important role in the fitness of locusts by conferring colonization resistance and by means of their volatile secretions (to be discussed below). This tight but not obligatory interaction may be routed in the function that these bacteria execute for their host. If more than one bacteria can produce the same essential product for the locust, this will push towards facultative, rather than obligatory, locust–bacteria interaction [28]. For example, if both Enterobacter and Klebsiella can produce cohesion promoting volatiles and to confer gut-colonization resistance, harboring one strain or the other will not affect the locust’s fitness, thus promoting the non-obligatory interaction, where the gut niche is colonized by the first suitable bacteria to be ingested by the locust.

The genus Weissella (Firmicutes: family Leuconostocaceae), was repeatedly found in the gut of L. migratoria [14,22,23], as well as following high-throughput sequencing of the desert locust’s gut microbiota [15,18]. The fact that this genus was absent from previous reports of the bacterial composition of locusts, may be attributed to difficulties in growing it in culture, which can be circumvented by advanced sequencing methods. Thus, it is highly plausible that additional important locust symbionts have not been cultivated to date and thus their effect on the host may be currently underappreciated.

2.2. The Female Reproductive System

As already noted, the organ at the spotlight of locust–bacteria research to date has been almost exclusively the gut. However, a recent report by Lavy et al. [16] focused on the reproductive system of the female desert locust and revealed the unique bacterial composition of different organs in this system. That study divided the female gonads into three main parts—accessory glands, ovaries, and spermatheca (Figure 1b), and used high throughput amplicon sequencing to analyze their bacterial composition. This resulted in the observation that the reproductive organs contained a consistent fraction of Corynebacterium, Brevibacterium and Micrococcus (Actinobacteria), along with Staphylococcus (Firmicutes) and Acinetobacter (Gammaproteobacteria) (Figure 1b). This unique bacterial composition of the female reproductive organs did not seem to be affected by mating, or by oviposition (during which the female extends her abdomen into the ground and oviposits at a depth of ~10 cm; [29]). The presence of the genus Brevibacterium in locusts had been reported only once prior to the aforementioned study: Bucher and Stephens [5] used a whole-body homogenate to examine the locust’s gut bacterial composition. Some bacteria isolated by them belonged to the genus Brevibacterium, leading these authors to consider it to be a gut-residing bacterium. However, later research, employing an isolated-gut approach, failed to find this bacterium in the locust gut. It is, therefore, possible that the origin of Brevibacterium, as described by Bucher and Stephens [5], was the locust reproductive system, and it was mistakenly described as a gut bacterium due to the experimental procedure.

2.3. The Cuticle

Our group recently sampled the abdominal cuticle of laboratory-reared S. gregaria in order to characterize its bacterial composition, as part of a larger study examining also the bacterial content of the fecal pellets of the same individuals [18]. It was then observed that the locust cuticle harbors a large fraction of what might be gut-associated bacteria, such as Weissella and other members of Firmicutes and Proteobacteria, which were also found in the feces (and which could have been amplified due to the crowded conditions in the cage). However, the bacteria residing on the locust integument also featured members of the phyla Bacteroidetes (Flavobacteriaceae and Weeksellaceae) and Actinobacteria (Corynebacteriaceae), which were unique to the external cuticle and were not present in the locust feces [18]. In another recent study [26], cultivation-dependent methods identified a species of Salmonella (Proteobacteria) externally on the head and body of S. gregaria, in agreement with our own observations noted above (Figure 1c).

3. Locust–Bacteria Mutualism

Bacterial symbionts have been repeatedly shown to play an important role in the biology of diverse insect species [30–33]. In a number of studies, bacteria were reported to augment aphid resistance to various stressors [31], to protect the gardens from Attine ants from pathogens [34], to protect wasp-larvae from potential pathogens [35], and more. The symbiotic nature of the locust–bacteria interaction has also been studied extensively, mostly in the desert locust and to some extent also in the migratory locust [10–18,21–23].

As noted above, the bacterial composition of the desert locust foregut was found to be roughly similar to that of the food ingested by the locusts. The bacterial community of the hindgut, however, differed from that in the food and the foregut, demonstrating a higher prevalence of Enterobacteriaceae cells [8]. This finding and the multiple subsequent confirmations of the stable presence of Enterobacteriaceae species in the locust hindgut suggested a selective basis for the interaction of S. gregria and bacteria from the Enterobacteriaceae family. Dillon and Charnley [10] hypothesized that the neutral pH of the hindgut (along with the presence of organic acids and plant remains) supports the proliferation of Enterobacteriaceae. This hypothesis was confirmed to some extent in L. migratoria by Shi et al. [14], who observed that acidification of the locust’s hindgut by entomopathogenic fungus reduced the bacterial colony-forming units (CFU) isolated from this organ. Those authors also demonstrated experimentally, by mimicking the acidification effect of the fungus on the hindgut, that the growth of locust-isolated Enterobacter sp. (Enterobacteriaceae) was almost completely inhibited when pH dropped from 6.3 (as in the hindgut of healthy L. migratoria) to 5.6 (reflecting the fungus impact). The growth of locust-isolated Weissella, Enterococcus, and Microbacterium was inhibited by the low pH of 5.6, though not to the same extent as the tested Enterobacter. Consequently, although the locusts had clearly not developed obligatory interactions with any specific bacteria, the specific conditions in their hindgut seem to have been selected for the proliferation of certain bacterial groups, including the Enterobacteriaceae family.

Native bacteria of the desert locust’s hindgut have been shown to augment host immunity through colonization resistance (CR), thereby making it less susceptible to pathogenic infections [12]. This is largely due to the bacterial synthesis of antifungal and antimicrobial phenolic compounds [35–37]. The antimicrobial nature of these compounds not only protects the locust from the establishment of pathogens, but also probably contributes to the selective conditions in the locust hindgut, creating favorable conditions for the anti-microbial tolerant native bacteria of the locust gut [10,12].

Two of the reported bacteria-secreted phenolic compounds, phenol and guaiacol, were also suggested as a dominant fraction of a cohesion pheromone, considered to help in maintaining the integrity of S. gregaria swarms [11,38]. Both phenol and guaiacol were shown to be produced by three bacterial species in the locust hindgut: Pantoea agglomerans, Klebsiella pneumoniae pneumoniae, and Enterobacter cloacae (all from the Enterobacteriaceae family), when incubated on axenic (i.e., bacteria-free) locust fecal pellets. The precursor for these phenolic compounds in the gut is the lignin-derived vanillic acid present in the locust’s food. Serratia marcescens a member of the Enterobacteriaceae which is not a regular inhabitant of the locust gut, did not produce the same phenolic volatiles [11], reinforcing the hypothesis of a mutual symbiosis between the host and specific bacterial species. Additional information on the phenolic compounds produced by the desert locust’s gut bacteria can be found in the excellent review by Dillon and Charnley [10].

This entry is adapted from the peer-reviewed paper 10.3390/insects11100655