Redox homeostasis is central to life, ranging from bioenergetics to metabolism and biological functions ^[1]. To defend against oxidative damage, organisms have evolved defenses that primarily rely on antioxidant enzymes, the supply of their substrates and repairing the damage. Antioxidant defenses can be enhanced through physiological signaling, dietary components and potential pharmaceutical interventions, thereby improving the capacity to scavenge oxidants and electrophiles. In 1954, Commoner et al. first described the occurrence of oxidative damage in a biological environment ^[2]. In 1985, the concept of “oxidative stress” was first applied to the biological logic system, which is defined as any oxidative damage in which excessive production of ROS or inadequate antioxidant defense occurs ^[3].

An imbalance between the production of oxidants and antioxidant defenses leads to damage to biological systems. This involves the chemistry of reactions of reactive species derived from oxygen, so-called ‘oxidative stress.’ Oxidative stress has been shown to participate in a variety of diseases, including cardiovascular disease, degenerative disease and cancer, and multiple mechanisms by which oxidants contribute to cellular damage have been revealed ^[4][5][6]. However, the degree of oxidative stress involved in the pathology of diseases is quite variable. This variability makes it difficult to improve the antioxidant effects of therapy. Most of the antioxidant defense in cells is provided not by either exogenous or endogenous small molecules acting as scavengers but by antioxidant enzymes using their specific substrates to reduce oxidants. The therapeutic use of small molecules has been disappointing, largely due to overoptimism and incorrect assumptions about how antioxidants work ^[7]. Furthermore, antioxidant enzymes react with oxidants thousands to millions of times faster than small molecules and provide the predominant antioxidant defense ^[7][8]. Therefore, the major therapeutic opportunities lie in preventing the production of oxidants that directly damage macromolecules, inhibiting the downstream signaling of oxidants that leads to inflammation or cell death, and increasing both antioxidant enzymes and their substrates. Currently, clinical trials based on this approach are underway ^[4]. A greater understanding of the mechanisms of action of antioxidants and where and when they are effective may provide a rational approach to addressing oxidative stress.

In the pharmaceutical industry, the approved medicinal products are mostly composed of a single molecule or combinations of single molecules whose pharmacological properties and safety are characterized during the preclinical phase and then validated in human trials ^[9]. The plants, animals, fungi, bacteria and archaea are a source of drugs of natural origin. Each of them contains hundreds of compounds, which belong to different classifications, such as diterpenoid, flavonoid, coumarin, steroid, hydrocarbon, carboxylic acid, ester, aldehyde, alcohol, ketone, ether, epoxide, phenol and so on ^[10]. Although many approved drugs isolated from natural sources have been proven to be involved in oxidation or antioxidation in experimental models and human studies ^[11], their mechanisms of action are usually not yet fully elucidated since it is difficult to identify the effective compounds and dosages. For example, researchers have demonstrated that Glycyrrhizae radix extract is beneficial in preventing oxidative damage in Caenorhabditis elegans (C. elegans). However, this extract is a mixture of all water-soluble compounds from Glycyrrhizae radix, so it is still unknown which compound plays an antioxidant role in this protective effect ^[12]. The composition of natural products is complex, thus posing a challenge to clarify their role in the oxidation and antioxidation process clearly. Along with the improved sensitivity of trace detection techniques (e.g., high-resolution mass spectrometry, instrumental neutron activation analysis, atomic absorption spectroscopy, ultra-performance liquid chromatography), an increasing number of newly discovered compounds have been isolated from natural products ^[13]. By means of plane and spatial structure analysis methods (e.g., nuclear magnetic resonance, atomic spectroscopy, circular dichroism spectrum, single crystal X-ray diffraction), the structures of these novel compounds can be resolved, making it possible to reveal the oxidation and antioxidation effects and mechanisms ^[14][15].

C. elegans was introduced to science as a model organism for development and neurobiology in 1965 ^[16]. Nematodes share approximately 60–80% of genes and 12 signaling pathways with humans ^[17][18], and notes on gene function can be obtained from the WormBase online consortium. C. elegans shows many advantages, such as self-fertilization, a short life cycle, a small and transparent body, ease of culture, simple operation and low cost, without limitations in ethics, showing great potential as an alternative model for the 3R principle ^[19][20]. Despite their simple structure, nematodes have complete muscle, subcutaneous tissue, nervous system, gut, gonads, glands and excretory system, and many basic physiological processes and oxidative stress responses of higher organisms are also highly conserved in nematodes ^[21][22]. Therefore, nematodes have great potential as models for evaluating the pharmacological and toxicological effects in humans ^[23], and researchers systematically summarize the advantages and disadvantages of C. elegans in the pharmacotoxicology research field and compare this model with other classical models, namely Drosophila, zebrafish, yeast, cell and mammalian in Table 1. In recent decades, nematodes have become very popular for high-throughput drug screening. This allows the drug discovery process to be studied throughout the life cycle and the manipulation of individual genes or genomes, such as N-ethyl-N-nitrosourea or ethyl methanesulfonate mutations, RNA interference (RNAi) and CRISPR ^[24]. Nematodes have been applied to the mechanism of action of addictive drugs ^[25], the pharmacological effects of neurodegenerative diseases such as Alzheimer’s disease (AD) drugs ^[26], and the neurotoxicity of anticancer drugs ^[27]. Currently, a large number of mutants have been used to study the molecular mechanism of effective components of natural products. For example, ursolic acid affects the stress response of nematodes by disturbing genes expressions of dopamine receptors ^[28][29], as also applies in the toxicological mechanism of the extract of Peganum harmala L. seeds ^[30], and the intestinal toxicity mechanism of Euphorbia factor L1 ^[31], etc. In addition, there are many reports on the effectiveness of antioxidants in C. elegans, as they can be used to establish an antioxidant stress response model for the assessment of antioxidant capacities in vivo ^{[32][33][34][35]}.

An oxidant is formed as a normal product at a relatively low concentration during aerobic metabolism but is produced at ascending rates under pathological conditions. A quasi-steady state is maintained via intricate patterns of oxidant and antioxidant balance, and the disruption will jeopardize normal biological functions ^[36][37]. Natural products represent rich sources of bioactive compounds for pharmacology and toxicology, and phytochemicals show great potential in oxidation or antioxidation, as proven by numerous experimental models and human studies. Moreover, aqueous and organic solvent extracts, as well as secondary metabolites, play vital roles in oxidative stress by means of different cellular and molecular mechanisms. As a rapidly developed model organism, C. elegans has been introduced into the oxidation and antioxidation assessment of natural products for decades. The results based on C. elegans correlate with traditional experiments on rodents and rabbits ^[38].

2. Establishment of Oxidative Stress Model in C. elegans