For various fields of chemistry, biotechnology, or medicine, it is most advantageous to utilize living cells immobilized in a stable matrix as biocatalysts. This ensures the effective use of their physiological characteristics for obtaining secondary metabolites or in biotransformation. For industrial application and design of sensors based on whole cells, it is necessary to create their high density in a sufficiently small volume of matter, which can be achieved by encapsulating/incorporating cells into polymer matrices. Polymers containing both inorganic elements and organic components play an important role in the development of encapsulation techniques. Immobilization in such matrices makes it possible to achieve the highest efficiency biocatalysts, which is promising for their practical application in biotechnology.

As a result of the encapsulation process, living microorganism cells are surrounded by the formed silica shells, and the “cell in shell” structure has formed. Because the cells are limited in space, their growth occurs; therefore, the characteristics of the hybrid materials do not change ^[22][23].

As a result of a two-stage sol-gel process, alcohol toxic to cells is released during hydrolysis. Mild synthesis conditions under which alcohol was removed from the system in the first stage under vacuum on a rotary evaporator ^[24] or with a gas flow were developed to reduce toxicity and increase biocompatibility. The toxic effects of alcohol can also be eliminated using aqueous precursors such as sodium silicate and colloidal silicon dioxide ^[25].

Reducing the toxic effects of both acids and alcohol on cells can be achieved using freeze drying. This process consists of freezing the cell suspension with ceramic powder and subsequent lyophilization. The addition of nutrients and cryoprotectants to the system while running the process at the optimum cooling rate improves cell viability. For example, the survival of Rhodococcus ruber was increased from 0.9% to 6.1% by the addition of trehalose solution ^[26] (Figure 3).

The traditional sol-gel process can be improved by introducing additives such as glycerol, glucose, and other sugars, or natural or synthetic polymers into the system to increase cell survival. They tend to increase the long-term stability of cells, as shown in the case of glycerol ^[27] and glucose ^[28]. Simultaneously, the immobilization process remains the same. These additives reduce the transparency of the matrix, which is important in the development of optical sensors.

With the exception of silica-based gels, microorganisms were encapsulated in oxide matrices of alumina, magnetite, titanium oxide, and zirconium ^{[29][30][31][32][33]}. Aqueous titanium and zirconium gels and their use for the encapsulation of microorganisms have been described ^{[28][30][34][35][36]}. Sols based on metal alkoxide were stabilized by the self-assembly of hydrophilic ligands, which ensured the formation of colorless, transparent aqueous sols. Encapsulated microorganisms were coated with a hydrated oxide and additionally included in the pores of the gel.

The immobilization of bacteria in a sol-gel matrix leads to stabilization of the catalytic activity and makes it possible to repeatedly or continuously use the biocatalyst. The integration of microorganisms into sol-gel structures removes many limitations that arise during the working with free cell systems ^{[21][37][38][39]}.

Over the last two decades, immobilization in a sol-gel matrix of microorganisms such as Escherichia coli ^{[19][24][29][38]}, Pseudomonas ^[40][41][42], Streptococcus ^[43], and Bacillus ^[44] has been intensively studied (Figure 6). During the formation of sol-gel materials, the release of lower alcohols (ethanol or methanol) often occurs. This is the main cause of mass mortality of bacterial cells, in contrast to yeast, which is less affected by alcohols ^[24]. Escherichia coli bacteria have been efficiently encapsulated in organosilicon matrices ^[45]. Bacteria were isolated from each other in a layer of sol-gel material but still exhibited enzymatic activity against some substrates. However, the long-term stability of bacteria was 1 month, with a survival rate of approximately 10% even with the formation of sol-gel matrices under near physiological conditions at the required temperature, pH and ionic strength of the solution. To increase the viability of bacteria, various organic compounds are added during matrix formation, such as polyvinyl alcohol, gelatin, and glycerol ^[46]. It was shown that glycerol allowed the maintenance of the metabolic activity of almost 50% of bacteria after 1 month.

Kim et al. immobilized Escherichia coli bacteria in a silica sol-gel matrix and demonstrated their biological activity retention ^[47]. The study of the obtained material structure was carried out in the presence of various organic components, which increased the long-term performance of the biomaterial. The immobilization of Escherichia coli bacteria is used to explore the stability during storage and long-term continuous processes ^[48], to study the formation of various structures and the functioning of enzyme preparations during their immobilization ^[13], to study the effect of the resulting alcohol distillation on increasing cell viability ^[24][49], and to assess the effect of stress factors on bacterial immobilization ^[29] (Figure 6).

Immobilization of microorganisms in sol-gel matrices can be considered an alternative for long-term storage of nodule bacteria of the genus Rhyzobium at room temperature ^[50]. Sodium silicate was used as a precursor by Diazs’ group. In a continuation of the study ^[46], glycerol was used as the organic component. The bacteria immobilized in the sol-gel matrix retained their viability and catalytic activity for up to 360 days of storage at room temperature. In addition, the silicon matrix has been shown to have the ability to protect bacteria from acid attack.

In view of their high abundance, cyanobacteria (Figure 4) are often used as model objects for studying various processes, including immobilization methods. In addition, they are important in biotechnology in the production of food additives, food, and pharmaceutical compounds and pigments, as well as in the production of biofuels and other products. The study of cyanobacteria encapsulation in a silicate matrix is described in ^[23].

Cells were immobilized in a sol-gel framework based on tetraethoxysilane (TEOS) under acid catalysis. Glycerol was used as an organic additive. As a result, cyanobacteria were encased in a porous organosilicon capsule, which, on the one hand, protects each cell from mechanical damage and, on the other hand, does not prevent the rapid diffusion of low molecular weight substances through the pores of the material ^[23].

A silica-based adsorbent biogel was created by incorporating the bacteria Pseudomonas sp. NCIB 9816-4 that degrade a wide range of aromatic contaminants. The adsorbent matrix was synthesized using the silica precursors methyltrimethoxysilane (MTMS) and tetramethoxysilane (TMOS) (Figure 5).

The encapsulated bacteria increase the rate of removal of the aromatic chemical mixture. Immobilized Pseudomonas bacteria have been successfully used to decolorize Remazol black, methylene orange, and benzyl orange, which are azo dyes commonly used in industrial processes ^[41] (Figure 6). The immobilized cells produced more than seven extracellular enzymes involved in the biodegradation of azo dyes. The reusability of immobilized bacteria has been evaluated through multiple experiments ^[41][42].

Immobilization of the methanotrophic bacterium Methylomonas sp. GYJ3 by the sol-gel technique enhances the activity of microorganisms at higher pH and temperature. However, the cells encaged by the sol-gel matrix based on MTMS had a lower activity compared to the activity of free cells ^[55]. At the same time, sol-gel matrices with immobilized Gram-positive Rhodococcus ruber bacteria demonstrated unchanged mechanical strength and good activity of immobilized cells when stored for several months at 4 °C ^[21]. The created ceramic composites can be reused for 12 months without loss of biological activity for bioremediation processes, as Rhodococcus spp. decompose a large number of pollutants that are difficult to oxidize, such as petroleum hydrocarbons, chlorinated, nitrogen-containing and other complex organic substances.

The immobilization of yeast cells in a sol-gel matrix attracts much attention, as the alcohol released during the sol-gel reactions is not as harmful to yeast as to bacteria. In addition, yeast cells are often used as templates for the formation of porous inorganic structures ^[35].

The possibility of obtaining channel-like meso/macroporous TiO₂, a potential anode material for lithium-ion batteries, has been described ^[28]. For this, a sol-gel process based on titanium tetraisopropoxide using yeast cells of Saccharomyces cerevisiae was utilized.

The first work on the immobilization of the Saccharomyces cerevisiae whole cells in sol-gel was published in 1989 ^[56][57]. Since then, some S. cerevisiae cells have been used as models for studying yeast viability after encapsulation in a sol-gel matrix based on tetraethoxysilane, tetramethoxysilane, and diethoxymethylsilane ^{[52][58][59][60]} (Figure 7). Yeast Saccharomyces cerevisiae cells genetically engineered to produce yellow fluorescent protein in response to galactose were encapsulated in polyglycerol silicate matrices. The matrix consisted of glycerol, TEOS, and titanium isopropoxide ^[31]. A biohybrid of nanosilica and the model organism Saccharomyces cerevisiae was synthesized to remove mercury from an aqueous solution ^[61]. The efficiency of biosorption of heavy metals by microbial biomass is mainly related to the structure of the microorganism’s cell wall. Therefore, the structure and properties of the cell surface determine the nature of the interaction between the microorganism and the metal cation. The walls of yeast cells are negatively charged due to the presence of functional groups such as amino groups and phosphate and hydroxyl groups, which are involved in the binding of heavy metals. It is well known that among the various reactive compounds associated with cell walls, extracellular polymeric substances such as exopolysaccharides have a great ability to form complexes with heavy metals. The biohybrid has been shown to exhibit high Hg(II) adsorption capacity, demonstrating a rapid removal of more than 98 ± 2% of this contaminant in 30 min. The synthesized biohybrid material can be easily regenerated, and the efficiency of Hg(II) removal can be maintained when reused. In addition, the encapsulation of Yarrowia lipolytica in silicon matrices based on TEOS enables the development of a heterogeneous biomaterial that not only has the ability to remove Cr(III) and Cr(VI) pollutants from water without special pretreatment and with high efficiency but also to dispose of hydrocarbons in aqueous conditions. This process is possible due to Yarrowia lipolytica’s ability to produce various enzymes (proteases, lipases, and esterases), emulsifiers, and surfactants. The resulting biohybrid material has the advantages of a hydrophobic and porous structure and is able to achieve almost 100% removal efficiency of chromium and n-hexadecane ions in an aqueous medium ^[62].

It was found that in a hybrid material formed by silicon dioxide, polyvinyl alcohol, and 4-vinylpyridine with immobilized cells of the yeast Trichosporon cutaneum, a biocompatible microenvironment is formed, which contributes to the preservation of the viability of encapsulated cells ^[63]. Arthroconidia that have formed in the extracellular material play an important role in maintaining the long-term viability of microorganisms, which may be related to their ability to withstand environmental stresses. A biosensor based on the encapsulated yeast Trichosporon cutaneum was used to analyze the biochemical oxygen consumption in contaminated effluents.

Therefore, materials synthesis technology by the sol-gel method can be effectively used for the immobilization of a wide range of microorganisms, including Gram-positive and Gram-negative bacteria, as well as different types of yeast. At the same time, the sol-gel approach provides a biocompatible environment that protects cells from external influences, regardless of the microorganism type.