Dairy Products through the Addition of Microalgae: History
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Microalgae are prokaryotic or eukaryotic photosynthetic microorganisms which have the ability to grow fast and to live under conditions not favorable to other species. They are attracting increasing attention, as their incorporation in foods and beverages can be a promising strategy to develop sustainable foods with improved nutritional profiles and a strong positive impacts on health. Despite the increasing market demand in plant-based foods, the popularity of fermented dairy foods has increased in the recent years since they are a source of microorganisms with health-promoting effects. In this context, the incorporation of microalgae in cheeses, fermented milks and other dairy products represents an interesting approach towards the development of innovative and added-value hybrid products based on animal proteins and enriched with vegetable origin ingredients recognized as extremely valuable sources of bioactive compounds.

  • microalgae
  • dairy products
  • bioactive compounds
  • legislation

1. Introduction

Microalgae are prokaryotic or eukaryotic photosynthetic microorganisms which have the ability to grow fast and to live under conditions not favorable to other species [1]. The biomass of these microorganisms is characterized for being a remarkable source of bioactive compounds and other products, which has led to a huge interest in their use in recent years [2][3]. Although approximately 10 million species of microalgae have been described in nature, so far only 50 of these species (mainly from Arthrospira (Spirulina), Chlorella, Porphyry, Nannochloropsis, Haematococcaceae, and Dunaliella genera) have been studied in detail in relation to their biotechnological use [4][5][6]. The nutritional composition of microalgae is very variable and depends enormously on the species and even within the species, depending on the growth conditions (composition of the medium, temperature and light regime) [7]. Table 1 shows the nutritional composition (protein, fat, carbohydrates, and minerals, among others) of the most studied microalgae species.
Table 1. Physicochemical composition of the most studied species of microalgae.

Physicochemical Composition

Species

 
 

Chlorella vulgaris1

Nannochloropsis gaditana2

Arthrospira platensis3

Auxenochlorella protothecoides4

Euglena gracilis5

Dunaliella bardawil6

Tetraselmis chuii7

Protein (% dry matter)

12–44

18–50

50–70

6–43

41–47

29–31

11–46

Lipid (% dry matter)

22–46

10–17

8–9.3

7–59

13–23

10–19

0.3–23

Carbohydrate (% dry matter)

24–39

15–31

13–48

15–35

34–43

11–12

30–54

Pigments

Lutein (mg/kg)

0.2–5

n.r

n.r

n.r

n.r

4.2–6.7

624

Chlorophyll (mg/L)

6–18

0.3–2.3

5–14

0.1–4

1–5.3

7.9–9.1

353–400

Phycocyanin (mg/mL)

n.r

n.r

0.5–2.3

n.r

n.r

n.r

n.r

Beta-carotene (mg/g)

n.r

0.1–2.9

n.r

0.1–1.1

0.1–52

0.8–1.5

0.1–1

Vitamins (mg/kg)

B2

20–34

25–62

34–81

n.r

n.r

n.r

5.3

B3

0.2–0.3

51–70

0.1–55

n.r

n.r

n.r

80

B9

0.7–1

17–26

2.6–7.9

n.r

n.r

n.r

200

B12

0.3–2.4

0.9–1.7

1.6 –3.2

n.r

n.r

0.42

78–195

E

n.r

n.r

n.r

n.r

0.2–1.6

1.5–2

0.2

C

n.r

n.r

n.r

n.r

0.9–1.3

1.8–2.2

0.8

Fatty acids (% total fatty acids)

C16:0 (palmitic)

20–30

13–41

43–57

11–25

14–16

15–17

19–36

C18:3 n-3 (alpha-linolenic)

22–24

0.9–3

1.3–23

2.4–30

0.1–0.3

22–31

22–28

C18:3 (linolenic)

26–28

0.3–7.4

14–19

22–35

n.r

3.2–3.7

n.r

C16:2 (hexadecadienoic)

12–23

0.1–2

2.2–6

0.4–3.5

1–2.5

12–14

1.8–5

C18:1 (oleic)

29–33

1.6–7.3

1–19

7.6–50

3.7–6.4

5.3–8.9

12.5–20

Information adapted from: 1 Ran et al. [8]; Mehariya et al. [9]; Rodrigues-Sousa et al. [10]; 2 Ran et al. [11]; Fattore et al. [12]; Nogueira et al. [13]; 3 Shanthi et al. [14]; Batista de Oliveira et al. [15]; Morais et al. [16]; 4 Xing et al. [17]; Polat et al. [18]; Bohutskyi et al. [19]; 5 Jung et al. [20]; Zhu et al. [21]; Kottuparambil et al. [22]; 6 Kumudha and Sarada [23]; Mixson Byrd and Burkholder [24]; Torres-Tiji et al. [7]; 7 Pereira et al. [25]; Schulze et al. [26]; Qazi et al. [27]. n.r—not reported.
The use of microalgae covers different areas, involving many applications. In the food industry, microalgae are used in the development of vegetarian and vegan foods as a substitute for macronutrients of animal origin, namely proteins [28][29], essential fatty acids and vitamins [5]. Furthermore, these microorganisms are employed to enrich different products, such as biscuits, nutritional bars, juices, pasta, breads, and dairy products [30][31].
In addition, microalgal biomass has been used in animal feed due to its high content of protein and carbohydrates, beyond improving the immune response and fertility in animals [32]. Another promising application of microalgae is their use in the medical field, mainly as a source of health beneficial compounds with anti-cancer, anti-inflammatory or anti-hypertensive properties [28][33]. Moreover, the use of microalgae extends to areas such as biofuel or bioplastic production, due to their high lipid and protein content [34][35][36].
In recent years, the development of new products with improved nutritional, structural and sensory characteristics has been highly demanded by consumers. Food industries are continually exploring the potential of new ingredients, and some of these innovative ingredients are referred as functional or nutraceutical ingredients, since besides their nutritional value, they also have benefits on the human body, reducing the risk of disease or improving consumers’ health [4][31]. In the last decades, there has been a rising interest in finding natural innovative, nutritive and sustainable sources to produce nutraceutical ingredients. In this sense, microalgae are considered as one of the promising sources of functional food ingredients, resulting from their large amounts of bioactive compounds [37][38]. Various studies have shown the impact of fortification with microalgal biomass on several food products such as pasta, bread, and cookies, among others, evidencing the great potential of these microorganisms, even at low levels, in the production of healthy foods [8][12][14].
On the other hand, dairy products are considered an excellent nutritional source and are widely consumed by a large part of the world’s population [39][40][41]. In addition, these products are characterized by having great benefits on human health, for instance, positive effects on bones and teeth [42][43], hypertension [44], cardiovascular diseases [45][46], gastrointestinal health [47][48], muscle repair after exercise and the immune system [49]. Moreover, fermented dairy products have been attracting special attention, as beyond their nutritional and sensory profile they are a potential source of probiotics with a remarkable impact in the food–gut axis. However, in recent years the consumption of dairy products has been decreasing, since there is skepticism among the general consumers about the health effects of dairy foods, and also an increasing public concern about their sustainability since they are products of animal origin. For this reason, the search for new, healthier and more sustainable solutions is essential.

2. Applications of Microalgal Biomass and Its Derivatives in Yogurt

Fermented milks as yogurt with probiotic microorganisms, presenting positive effects on health, are currently in high demand. Therefore, it is possible to find them in different types of markets everywhere worldwide. In order to increase their beneficial properties, there are some studies focused on the incorporation of different matrices rich in nutraceutical compounds in fermented milks, for instance, microalgal biomass or its derivatives (Table 2). In these studies, two ways to add microalgal biomass to yogurt were identified: the addition to the milk before the fermentation process (i), or the addition to the final product after the fermentation process (ii). The choice of each approach can affect the physicochemical, sensory or mechanical characteristics of the final product.
Table 2. Studies on the application of microalgal biomass or derivates in yogurt. BFP—before the fermentation process; AFP—after the fermentation process.

Microalgae or Derivate

Addition Rate

Physicochemical, Sensory, Rheology, Textural or Functional Characteristics

References

Chlorella vulgaris

0.25, 0.50 and 1% (w/v) BFP

Final acidity (°D) and final redox potential (mv) were higher than the controls, pH and acetic acid (%) values were not different compared to the controls. Oral texture and feel in the mouth, appearance and nonoral texture were lower than the control.

Beheshtipour et al. [50]

Isochrysis galbana

2% (w/w) AFP

Protein and ash percentages were higher than the controls, lipid content (%) was not different compared to the control. Levels of ω3-fatty acids were higher than the control.

Matos et al. [51]

Pavlova lutheri

0.25 and 0.5% (w/v) AFP

Moisture, carbohydrate, protein and fat contents were not different compared to the control. pH values during storage (28 days) were similar to the control. Addition rate in the treatments was negatively correlated with color, liking of flavor, liking of texture and overall acceptability.

Robertson et al. [52]

Phycocyanin from Arthrospira platensis

2, 4 and 8% (w/w) BFP

Treatments showed pH values higher than the control during 21 days of storage. Supplemented yogurts showed a lower viscosity compared to the control during 21 days of storage. Treatment with 4% of phycocyanin was the most accepted by the panelists.

Mohammadi et al. [53]

Arthrospira platensis

0.25, 0.50, 0.75 and 1% (w/v) BFP

Total solids, protein, ash and fat contents were higher than the control. There was a reduction in pH values of the treatments compared to the control. Fortified samples exhibited lower firmness compared to the control. Yogurts containing 2% of A. platensis had the highest score for acceptability.

Barkallah et al. [54]

Arthrospira platensis

1.% (w/w) AFP

Moisture, fat, protein, lactose, and ash levels were higher compared to the control. pH values in fortified samples were greater than the control as well.

Da Silva et al. [55]

Arthrospira platensis

0.13, 0.25, 0.38 and 0.5% (w/v) BFP

Acidity levels in fortified yogurt were greater than the control during 16 days of storage. Overall acceptability decreased with higher amounts of A. platensis. The antioxidant capacity was reduced during storage.

Alizadeh et al. [56]

Arthrospira platensis

1% (w/w) BFP

Ash, total solid, fat, and protein contents had an increase compared to the control. There were no significative changes in the acidity and pH values. Total phenolic content and total antioxidant activity were increased in treatments with A. platensis. Apparent viscosity values of fortified samples were greater than the control.

Atallah et al. [57]

Spirulina platensis

0.1, 0.3 and 0.5% (w/v) BFP

Solid content, protein, fat, ash, carbohydrate and acidity levels in supplemented yogurts were higher than the control. There was a reduction in the pH values compared to the control. There was an increase in hardness and viscosity values of fortified samples compared to the control.

Bchir et al. [58]

3. Applications of Microalgal Biomass and Its Derivatives in Ice Cream

Ice cream is a dairy product that has a great consumption worldwide because of its nutritional properties and refreshing effect, especially in warmer weather days. This product is made from milk, sweeteners, stabilizers, emulsifiers, flavoring and coloring agents [59]. Some studies have shown the use of microalgal biomass in ice cream due to the high presence of pigments and compounds with stabilizing roles. Since food dyes have become common in the food production industry, there has been a debate about the harmful effects of artificial food colors. Therefore, the use of natural and functional pigments has increased in the recent years [59]. Table 3 presents some research on the incorporation of microalgae and their derivatives in ice cream.
Table 3. Studies on the application of microalgal biomass or derivates in ice cream.

Microalgae or Derivate

Addition Rate

Physicochemical, Sensory, Rheological, Textural or Functional Characteristics

References

Nannochloropsis oculata

0.1, 0.2 and 0.3% (w/w)

Fortified samples were greenish in color. There were no changes in the melting behavior of fortified samples. Consistency index (K) values of the samples were close to the control.

Durmaz et al. [59]

Arthrospira platensis

0.075, 0.15, 0.23 and 0.3% (w/w)

Acidity in supplemented ice cream was increased compared to the control. pH values of fortified samples were lower than the control sample. Higher amounts of microalgae resulted in a decrease of the viscosity. Overrun in supplemented samples was enhanced compared to control.

Malik et al. [60]

Arthrospira platensis

Pure and microencapsulated with maltodextrin or Arabic gum

Protein, fat and total solid were increased in ice cream with microencapsulated or pure Spirulina compared to control. Overall acceptability was higher in ice cream without microencapsulated or pure Spirulina. Melting time in samples with pure microalgae was lower than samples with microencapsulated Spirulina.

Balensiefer et al. [61]

Arthrospira platensis

0.6 and 1.2%

Total solid, protein and fat content were increased in enriched ice cream compared to control. Ice cream overrun and melting point were higher in fortified samples. Sensory analysis showed that the panelists preferred ice cream without microalgae.

Agustini et al. [62]

Diacronema vlkianum

0.1, 0.2 and 0.3% (w/w)

Supplemented ice cream was greenish in color. The panelists found a bitter taste in enriched samples. Ice cream with microalgae showed lower K values than the control.

Durmaz et al. [59]

Phycocyanin from Arthrospira platensis

0.025%

Fortified ice cream was bluish in color (negative values of b*) whereas control samples were yellowish in color (positive values of b*). Antioxidant capacity of supplemented samples was improved after digestion compared to control.

Campos et al. [63]

Porphyridium cruentum

0.1, 0.2 and 0.3% (w/w)

Protein, fat and total solid were increased in ice cream with microencapsulated or pure Spirulina compared to control. Phenolic compounds increased with greater amounts of microalgae. A higher quantity of microalgae adversely affected the ice cream general sensory parameters.

Durmaz et al. [59]

Phycocyanin from Arthrospira platensis

0.013%

There was no difference in the fat content of supplemented samples compared to control. Melting time in samples with phycocyanin was lower compared to control. Overall acceptability was higher in non-fortified samples.

Rodrigues et al. [64]

4. Applications of Microalgal Biomass in Cheeses

Cheese is a highly consumed dairy product around the world with a remarkable variety of aromas and shapes associated with the interaction of milk proteins, carbohydrates and fat, and the effect of bacteria in raw milk initiates microorganisms and probiotics [65]. In order to improve the sensory, nutritional and functional characteristics of cheeses, in recent years, studies have been carried out on the incorporation of microalgal biomass. Table 4 presents some of more recent research.
Table 4. Studies on the application of microalgal biomass in cheeses.

Microalgae

Addition Rate

Physicochemical, Sensory, Rheology, Textural or Functional Characteristics

References

Chlorella vulgaris

1, 2 and 3% (w/w)

There were significant differences between the control and cheese analogue enhanced by 3% C. vulgaris biomass in all the chemical components (moisture, fat, carbohydrate and salt content). The microalgae protein and carbohydrates promoted the increase of firmness and the decrease of oil separation indexes of the cheeses.

Mohamed et al. [66]

Chlorella vulgaris

2, 4 and 6% (w/w)

The pH of the cheeses increased with the percentage of microalgae added. The addition of microalgae to the processed cheese increased the degree of meltability compared with the control sample before and after storage.

Tohamy et al. [67]

Arthrospira platensis

0.5, 1 and 1.5% (w/w)

The increase in the amount of microalgae led to a reduction in moisture and an increase in protein and fat content in soft cheese. Cheeses fortified with Spirulina showed higher values of β-carotene than then control.

Agustini et al. [62]

Arthrospira platensis

0.25, 0.5 and 1% (w/w)

There was an increase in the protein and fat content in supplemented samples compared to control. Cheeses with 0.25% and 0.5% incorporated Spirulina were mostly preferred by the panelists.

Bosnea et al. [68]

Arthrospira maxima

1, 2 and 3% (w/w).

pH of fortified samples decreased slightly compared to the control. Fat, protein and solid total content were increased in samples with 3% of microalgae. Antioxidant capacity was enhanced in supplemented samples at storage compared to the control. Overall acceptability had high scores for all treatments and control.

Mohamed et al. [69]

Arthrospira platensis

0.5, 1 and 1.5% (w/w)

Protein and ash content of enriched cheeses were not affected by microalgae addition. The L* values of Spirulina-fortified samples decreased by increasing microalgae concentration. Spirulina-fortified samples showed significantly lower degrees of hardness than the control, both at the beginning and end of storage.

Golmakani et al. [70]

Arthrospira platensis

0.5, 1 and 1.5% (w/w)

Fat and protein content of the supplemented cheeses was improved by microalgae addition compared to the control. The addition of microalgae to the cheese increased the phenolic compound and flavonoid content and also the antioxidant capacity.

Mohamed [71]

5. Other Dairy Products

The effect of the incorporation of microalgae has also been studied in some dairy products not included in the aforementioned categories, for instance: fermented milk powder, kefir, buttermilk beverage and Labenah (a product originated in the Middle East considered as a hybrid mixture between cheese and yogurt).
Vlasenko et al. [72] developed a fermented beverage based on buttermilk enriched with A. platensis at 1.0, 1.5, 2.0 and 2.5% (w/w) and the results indicated that treatments with percentages of microalgal biomass lower than 2% showed acceptable acidity values (75–80 °T); however, treatments with algae concentrations greater than 2% resulted in very high acidity values (87–90 °T). Due to a higher substrate concentration, lactic acid bacteria were able to produce a higher amount of lactic acid during fermentation [73]. The fat content value for all treatments and the control was 0.4% (p > 0.05).
Martelli et al. [74] reported the effect of adding A. platensis (0.25 and 0.5% w/v) and Lactobacillus bulgaricus and Streptococcus thermophilus in reconstituted fermented milk powder (10% w/v), and it was observed that there was a significant decrease in the pH values (4.3 and 4.1) when the microalgae concentration was increased. This behavior is similar to that reported by Varga et al. [75], who observed a decrease in pH values in milk containing A. platensis and inoculated with S. thermophilus and L. bulgaricus when the algae concentration was increased. Likewise, the authors studied the effect of microalgal biomass addition on rheological behavior in terms of flow index (n) and consistency index (K). The results showed that there was a decrease in K values (2.56 and 1.77 Pa.sn) with the addition of 0.25 and 0.5% A. platensis, related to a reduction in the consistency when compared to the control sample (product without microalgae). The values of flow index (n) were increased with microalgae addition, ranging from 0.366 to 0.439, revealing a reduction in the shear thinning behavior.
Mohamed et al. [76] developed a high-quality protein Labenah enriched with A. platensis (0.5% w/w), and the results indicated that there was a significant increase in protein content (13.08% w/w) when compared to a control sample of 10.60% w/w. Microalgal biomass of A. platensis is known to have high levels of protein [14][15], and therefore its incorporation in Labenah resulted in an increase in the content value of this macronutrient. A similar trend was observed by Laela et al. [77], who reported a protein content of 5.53% in kefir fortified with 2% A. platensis compared to 4.02% in the control sample. In addition, the effect of storage on the acidity level of Labenah was also evaluated. Mohamed et al. [76] observed that there was an increase in the acidity values of this product, going from 2.2% (day 0) to 2.8% lactic acid (day 27) in samples enriched with A. platensis (0.5% w/w). This increase was more significant than in the control sample (from 1.7 to 1.8% lactic acid on day 0 and 27, respectively), which indicates that lactic acid production was beneficially influenced by the addition of microalgal biomass.

This entry is adapted from the peer-reviewed paper 10.3390/foods11050755

References

  1. Mata, T.M.; Martins, A.A.; Caetano, N.S. Microalgae for biodiesel production and other applications: A review. Renew. Sustain. Energy Rev. 2010, 14, 217–232.
  2. Vuppaladadiyam, A.; Prinsen, P.; Raheem, A.; Luque, R.; Zhao, M. Sustainability analysis of microalgae production systems: A review on resource with unexploited high-value reserves. Environ. Sci. Technol. 2018, 52, 14031–14049.
  3. Arashiro, L.T.; Boto-Ordóñez, M.; Van Hulle, S.W.H.; Ferrer, I.; Garfí, M.; Rousseau, D.P.L. Natural pigments from microalgae grown in industrial wastewater. Bioresour Technol. 2020, 303, 122894.
  4. Pina-Pérez, M.C.; Brück, W.M.; Brück, T.; Beyrer, M. The Role of Alternative and Innovative Food Ingredients and Products in Consumer Wellness. In Microalgae as Healthy Ingredients for Functional Foods, 1st ed.; Galanakis, C., Ed.; Academic Press: London, UK, 2019; pp. 103–137.
  5. Adarme-Vega, T.C.; Lim, D.K.Y.; Timmins, M.; Vernen, F.; Li, Y.; Schenk, P.M. Microalgal biofactories: A promising approach towards sustainable omega-3 fatty acid production. Microb. Cell Factories 2012, 111, 1–10.
  6. Li, Z.; Anbuchezhian, R.; Karuppiah, V. Prospect of marine algae for production of industrially important chemicals. In Algal Biorefinery an Integr Approac, 1st ed.; Debabrata, D., Ed.; Springer: New Delhi, India, 2016; pp. 95–217.
  7. Torres-Tiji, Y.; Fields, F.J.; Mayfield, S.P. Microalgae as a future food source. Biotechnol. Adv. 2020, 41, 107536.
  8. Ran, C.; Zhou, X.; Yao, C.; Zhang, Y.; Kang, W.; Liu, X. Swine digestate treatment by prior nitrogen-starved Chlorella vulgaris: The effect of over-compensation strategy on microalgal biomass production and nutrient removal. Sci. Total Environ. 2021, 768, 1444–1462.
  9. Mehariya, S.; Goswami, R.K.; Karthikeysan, O.P.; Verma, P. Microalgae for high-value products: A way towards green nutraceutical and pharmaceutical compounds. Chemosphere 2021, 280, 130553.
  10. Rodrigues-Sousa, A.E.; Nunes, I.V.O.; Muniz-Junior, A.B.; Carvalho, J.C.M.; Mejia-da-Silva, L.C.; Matsudo, M.C. Nitrogen supplementation for the production of Chlorella vulgaris biomass in secondary effluent from dairy industry. Biochem. Eng. J. 2021, 165, 107818.
  11. Safi, C.; Cabas Rodriguez, L.; Mulder, W.J.; Engelen-Smit, N.; Spekking, W.; van den Broek, L.A.M. Energy consumption and water-soluble protein release by cell wall disruption of Nannochloropsis gaditana. Bioresour Technol. 2017, 239, 204–210.
  12. Fattore, N.; Bellan, A.; Pedroletti, L.; Vitulo, N.; Morosinotto, T. Acclimation of photosynthesis and lipids biosynthesis to prolonged nitrogen and phosphorus limitation in Nannochloropsis gaditana. Algal Res. 2021, 58, 102368.
  13. Nogueir, N.; Nascimento, F.J.A.; Cunha, C.; Cordeiro, N. Nannochloropsis gaditana grown outdoors in annular photobioreactors: Operation strategies. Algal Res. 2020, 48, 101913.
  14. Shanthi, G.; Premalatha, M.; Anantharaman, N. Potential utilization of fish waste for the sustainable production of microalgae rich in renewable protein and phycocyanin-Arthrospira platensis/Spirulina. J. Clean Prod. 2021, 294, 126106.
  15. Batista de Oliveira, T.T.; Miranda dos Reis, I.; Bastos de Souza, M.; da Silva Bispo, E.; Fonseca Maciel, L.; Druzian, J.I. Microencapsulation of Spirulina sp. LEB-18 and its incorporation in chocolate milk: Properties and functional potential. LWT 2021, 148, 111674.
  16. Morais, E.G.; de Druzian, J.I.; Nunes, I.L.; Morais, M.G.; Costa, J.A.V. Glycerol increases growth, protein production and alters the fatty acids profile of Spirulina (Arthrospira) sp. LEB 18. Process Biochem. 2019, 76, 40–45.
  17. Xing, G.L.; Yuan, H.L.; Yang, J.S.; Li, J.Y.; Gao, Q.X.; Li, W.L. Integrated analyses of transcriptome, proteome and fatty acid profilings of the oleaginous microalga Auxenochlorella protothecoides UTEX 2341 reveal differential reprogramming of fatty acid metabolism in response to low and high temperatures. Algal Res. 2018, 33, 16–27.
  18. Polat, E.; Yüksel, E.; Altınbaş, M. Effect of different iron sources on sustainable microalgae-based biodiesel production using Auxenochlorella protothecoides. Renew Energy 2020, 162, 1970–1978.
  19. Bohutskyi, P.; Ketter, B.; Chow, S.; Adams, K.J.; Betenbaugh, M.J.; Allnutt, F.C.T. Anaerobic digestion of lipid-extracted Auxenochlorella protothecoides biomass for methane generation and nutrient recovery. Bioresour. Technol. 2015, 183, 229–239.
  20. Jung, J.M.; Kim, J.Y.; Jung, S.; Choi, Y.E.; Kwon, E.E. Quantitative study on lipid productivity of Euglena gracilis and its biodiesel production according to the cultivation conditions. J. Clean Prod. 2021, 291, 125218.
  21. Zhu, J.; Tan, X.; Hafid, H.S.; Wakisaka, M. Enhancement of biomass yield and lipid accumulation of freshwater microalga Euglena gracilis by phenolic compounds from basic structures of lignin. Bioresour. Technol. 2021, 321, 124441.
  22. Kottuparambil, S.; Thankamony, R.L.; Agusti, S. Euglena as a potential natural source of value-added metabolites. A review. Algal Res. 2019, 37, 154–159.
  23. Kumudha, A.; Sarada, R. Characterization of vitamin B12 in Dunaliella salina. J. Food Sci. Technol. 2016, 53, 888.
  24. Mixson Byrd, S.; Burkholder, J.A.M. Environmental stressors and lipid production in Dunaliella spp. II. Nutrients, pH, and light under optimal or low salinity. J. Exp. Mar. Biol. Ecol. 2017, 487, 33–44.
  25. Pereira, H.; Silva, J.; Santos, T.; Gangadhar, K.N.; Raposo, A.; Nunes, C. Nutritional potential and toxicological evaluation of Tetraselmis sp. CtP4 microalgal biomass produced in industrial photobioreactors. Molecules 2019, 24, 3192.
  26. Schulze, P.S.C.; Pereira, H.G.C.; Santos, T.F.C.; Schueler, L.; Guerra, R.; Barreira, L.A. Effect of light quality supplied by light emitting diodes (LEDs) on growth and biochemical profiles of Nannochloropsis oculata and Tetraselmis chuii. Algal Res. 2016, 16, 387–398.
  27. Qazi, W.M.; Balance, S.; Uhlen, A.K.; Kousoulaki, K.; Haugen, J.E.; Rieder, A. Protein enrichment of wheat bread with the marine green microalgae Tetraselmis chuii–Impact on dough rheology and bread quality. LWT 2021, 143, 111115.
  28. Morais Junior, W.G.; Gorgich, M.; Corrêa, P.S.; Martins, A.A.; Mata, T.M.; Caetano, N.S. Microalgae for biotechnological applications: Cultivation, harvesting and biomass processing. Aquaculture 2020, 528, 7355–7362.
  29. Becker, E.W. Micro-algae as a source of protein. Biotechnol Adv. 2007, 25, 207–210.
  30. Chacón-Lee, T.L.; González-Mariño, G.E. Microalgae for “healthy” foods-possibilities and challenges. Compr. Rev. Food Sci. Food Saf. 2010, 9, 655–675.
  31. Bernaerts, T.M.M.; Gheysen, L.; Foubert, I.; Hendrickx, M.E.; Van Loey, A.M. The potential of microalgae and their biopolymers as structuring ingredients in food: A review. Biotechnol. Adv. 2019, 37, 107419.
  32. Gill, I.; Valivety, R. Polyunsaturated fatty acids, part 1: Occurrence, biological activities and applications. Trends Biotechnol. 1997, 15, 401–409.
  33. Raja, R.; Coelho, A.; Hemaiswarya, S.; Kumar, P.; Carvalho, I.S.; Alagarsamy, A. Applications of microalgal paste and powder as food and feed: An update using text mining tool. Beni-Suef Univ. J. Basic Appl. Sci. 2018, 7, 740–747.
  34. López Rocha, C.J.; Álvarez-Castillo, E.; Estrada Yáñez, M.R.; Bengoechea, C.; Guerrero, A.; Orta Ledesma, M.T. Development of bioplastics from a microalgae consortium from wastewater. J. Environ. Manag. 2020, 263, 110353.
  35. Khoo, K.S.; Chew, K.W.; Yew, G.Y.; Leong, W.H.; Chai, Y.H.; Show, P.L. Recent advances in downstream processing of microalgae lipid recovery for biofuel production. Bioresour. Technol. 2020, 304, 122996.
  36. Ndimba, B.K.; Ndimba, R.J.; Johnson, T.S.; Waditee-Sirisattha, R.; Baba, M.; Sirisattha, S. Biofuels as a sustainable energy source: An update of the applications of proteomics in bioenergy crops and algae. J. Proteom. 2013, 93, 234–244.
  37. Khemiri, S.; Khelifi, N.; Nunes, M.C.; Ferreira, A.; Gouveia, L.; Smaali, I. Microalgae biomass as an additional ingredient of gluten-free bread: Dough rheology, texture quality and nutritional properties. Algal Res. 2020, 50, 101998.
  38. Matos, J.; Cardoso, C.; Bandarra, N.M.; Afonso, C. Microalgae as healthy ingredients for functional food: A review. Food Funct. 2017, 8, 2672–2685.
  39. Tunick, M.H.; Van Hekken, D.L. Dairy products and health: Recent insights. J. Agric. Food Chem. 2015, 63, 9381–9388.
  40. Thomas, J.; Beeren, C. Consumer perception of additives in dairy products. In Reference Module in Food Science, 2nd ed.; Fuquay, J., Ed.; Academic Press: San Diego, CA, USA, 2011; pp. 41–48.
  41. Cosentino, C.; Colonna, M.A.; Musto, M.; Dimotta, A.; Freschi, P.; Tarricone, S.; Ragni, M.; Paolino, R. Effects of dietary supplementation with extruded linseed and oregano in autochthonous goat breeds on the fatty acid profile of milk and quality of Padraccio cheese. J. Dairy Sci. 2021, 104, 1445–1453.
  42. Moore, L.L.; Bradlee, M.L.; Gao, D.; Singer, M.R. Effects of average childhood dairy intake on adolescent bone health. J. Pediatr. 2008, 153, 667–673.
  43. Iuliano, S.; Hill, T.R. Dairy foods and bone accrual during growth and development. In Milk and Dairy Foods, 1st ed.; Givens, D., Ed.; Academic Press: San Diego, CA, USA, 2011; pp. 299–322.
  44. Lana, A.; Banegas, J.R.; Guallar-Castillón, P.; Rodríguez-Artalejo, F.; Lopez-Garcia, E. Association of dairy consumption and 24-hour blood pressure in older adults with hypertension. Am. J. Med. 2018, 131, 1238–1249.
  45. Kouvari, M.; Panagiotakos, D.B.; Chrysohoou, C.; Georgousopoulou, E.N.; Yannakoulia, M.; Tousoulis, D. Dairy products, surrogate markers, and cardiovascular disease; a sex-specific analysis from the ATTICA prospective study. Nutr. Metab. Cardiovasc. Dis. 2020, 30, 2194–2206.
  46. Kris-Etherton, P.M.; Grieger, J.A.; Hilpert, K.F.; West, S.G. Milk products, dietary patterns and blood pressure management. J. Am. Coll. Nutr. 2009, 28, 103S–119S.
  47. Rosa, M.C.; Carmo, M.R.S.; Balthazar, C.F.; Guimarães, J.T.; Esmerino, E.A.; Freitas, M.Q. Dairy products with prebiotics: An overview of the health benefits, technological and sensory properties. Int. Dairy J. 2021, 117, 105009.
  48. Scott, K.P.; Grimaldi, R.; Cunningham, M.; Sarbini, S.R.; Wijeyesekera, A.; Tang, M.L.K. Developments in understanding and applying prebiotics in research and practice—an ISAPP conference paper. J. Appl. Microbiol. 2020, 127, 934–949.
  49. Virgilio, N.; De Donno, R.; Bandini, E.; Napolitano, A.; Fogliano, V.; Vitaglione, P. Milk protein enriched beverage reduces post-exercise energy intakes in women with higher levels of cognitive dietary restraint. Food Res. Int. 2019, 118, 58–64.
  50. Beheshtipour, H.; Mortazavian, A.M.; Haratian, P.; Khosravi-Darani, K. Effects of Chlorella vulgaris and Arthrospira platensis addition on viability of probiotic bacteria in yogurt and its biochemical properties. Eur. Food Res. Technol. 2012, 235, 719–728.
  51. Matos, J.; Afonso, C.; Cardoso, C.; Serralheiro, M.L.; Bandarra, N.M. Yogurt enriched with Isochrysis galbana: An innovative functional food. Foods 2021, 10, 1458.
  52. Robertson, R.C.; Gracia Mateo, M.R.; O’Grady, M.N.; Guihéneuf, F.; Stengel, D.B.; Ross, R.P. An assessment of the techno-functional and sensory properties of yoghurt fortified with a lipid extract from the microalga Pavlova lutheri. Innov. Food Sci. Emerg. Technol. 2016, 37, 237–246.
  53. Mohammadi-Gouraji, E.; Soleimanian-Zad, S.; Ghiaci, M. Phycocyanin-enriched yogurt and its antibacterial and physicochemical properties during 21 days of storage. LWT 2019, 102, 230–236.
  54. Barkallah, M.; Dammak, M.; Louati, I.; Hentati, F.; Hadrich, B.; Mechichi, T. Effect of Spirulina platensis fortification on physicochemical, textural, antioxidant and sensory properties of yogurt during fermentation and storage. LWT 2017, 84, 323–330.
  55. Silva, S.C.; Fernandes, I.P.; Barros, L.; Fernandes, Â.; Alves, J.M.; Calhelha, R.C. Spray-dried Spirulina platensis as an effective ingredient to improve yogurt formulations: Testing different encapsulating solutions. J. Funct. Foods. 2019, 60, 103427.
  56. Alizadeh Khaledabad, M.; Ghasempour, Z.; Kia, E.M.; Bari, M.R.; Zarrin, R. Probiotic yoghurt functionalised with microalgae and Zedo gum: Chemical, microbiological, rheological and sensory characteristics. Int. J. Dairy Technol. 2020, 73, 67–75.
  57. Atallah, A.; Morsy, O.; Dalia, G. Characterization of functional low-fat yogurt enriched with whey protein concentrate, Ca-caseinate and Spirulina. Int. J. Food Prop. 2020, 23, 78–91.
  58. Bchir, B.; Felfoul, I.; Bouaziz, M.A.; Gharred, T.; Yaich, H.; Noumi, E. Investigation of physicochemical, nutritional, textural, and sensory properties of yoghurt fortified with fresh and dried Spirulina (Arthrospira platensis). Int. Food Res. J. 2019, 26, 65–76.
  59. Durmaz, Y.; Kilicli, M.; Toker, O.S.; Konar, N.; Palabiyik, I.; Tamtürk, F. Using spray-dried microalgae in ice cream formulation as a natural colorant: Effect on physicochemical and functional properties. Algal Res. 2020, 47, 101811.
  60. Malik, P.; Kempanna, C.; Paul, A. Quality characteristics of ice cream enriched with Spirulina powder. Int. J. Food Nutr. Sci. 2012, 2, 44–50.
  61. Balensiefer, C.; Tiepo, V.; Gottardo, F.M.; Bertol, C.D.; Reinehr, C.O.; Colla, L.M. Addition of Spirulina platensis in handmade ice cream: Phisicochemical and sensory effects. Braz. J. Dev. 2021, 7, 88106–88123.
  62. Agustini, T.W.; Ma’ruf, W.F.; Widayat, A.; Suzery, M.; Hadiyanto, L.; Benjakul, S. Application of Spirulina platensis on ice cream and soft cheese with respect to their nutritional and sensory perspectives. J. Teknol. 2016, 78, 245–251.
  63. Campos Assumpção de Amarante, M.; Cavalcante Braga, A.R.; Sala, L.; Juliano, B.; Kalil, S. Colour stability and antioxidant activity of C-phycocyanin-added ice creams after in vitro digestion. Food Res. Int. 2020, 137, 109602.
  64. Rodrigues, E.F.; Vendruscolo, L.P.; Bonfante, K.; Reinehr, C.O.; Colla, E.; Colla, L.M. Phycocyanin as substitute for texture ingredients in ice creams. Br. Food J. 2020, 122, 693–707.
  65. Cosentino, C.; Faraone, D.; Paolino, R.; Freschi, P.; Musto, D. Short communication: Sensory profile and acceptability of a cow milk cheese manufactured by adding jenny milk. J. Dairy Sci. 2016, 99, 228–233.
  66. Mohamed, A.G.; Abo-El-Khair, B.E.; Shalaby, S.M. Quality of novel healthy processed cheese analogue enhanced with marine microalgae Chlorella vulgaris biomass. World Appl. Sci. J. 2013, 23, 914–925.
  67. Tohamy, M.M.; Ali, M.A.; Shaaban, H.A.G.; Mohamad, A.G.; Hasanain, A.M. Production of functional spreadable processed cheese using Chlorella vulgaris. Acta Sci. Pol. Technol. Aliment. 2018, 17, 347–358.
  68. Bosnea, L.; Terpou, A.; Pappa, E.; Kondyli, E.; Mataragas, M.; Markou, G. Incorporation of Spirulina platensis on traditional greek soft cheese with respect to its nutritional and sensory perspectives. Proceedings 2020, 70, 99.
  69. Mohamed, A.G.; Abd El-Salam, B.A.E.Y.; Gafour, W.A.E.M. Quality characteristics of processed cheese fortified with Spirulina powder. Pak. J. Biol. Sci. 2020, 23, 533–541.
  70. Golmakani, M.T.; Soleimanian-Zad, S.; Alavi, N.; Nazari, E.; Eskandari, M.H. Effect of Spirulina (Arthrospira platensis) powder on probiotic bacteriologically acidified feta-type cheese. J. Appl. Phycol. 2019, 31, 1085–1094.
  71. Mohamed, A.; Darwish, I. Physicochemical properties, bioactive compounds and antioxidant activity of Kareish cheese fortified with Spirulina platensis. World J. Dairy Food Sci. 2017, 12, 71–78.
  72. Vlasenko, I.; Bandura, V.; Semko, T.; Fialkovska, L.; Ivanishcheva, O.; Palamarchuk, V. Innovative approaches to the development of a new sour milk product. Slovak J. Food Sci. 2021, 15, 970–981.
  73. Manuelian, C.; Currò, S.; Penasa, M.; Cassandro, M.; De Marchi, M. Characterization of major and trace minerals, fatty acid composition, and cholesterol content of protected designation of origin cheeses. J. Dairy Sci. 2017, 100, 384–395.
  74. Martelli, F.; Alinovi, M.; Bernini, V.; Gatti, M.; Bancalari, E. Arthrospira platensis as natural fermentation booster for milk and soy fermented beverage. Foods 2020, 9, 350.
  75. Varga, L.; Szigeti, J.; Kovács, R.; Földes, T.; Buti, S. Influence of a Spirulina platensis biomass on the microflora of fermented ABT milks during storage (R1). J. Dairy Sci. 2002, 85, 1031–1038.
  76. Mohamed, H.; Sayed, E.; Mohamed, Z.; Gaber, A. Applicability of using edible algae (Spirulina platensis) to prepare high protein quality Labenah. J. Biol. Sci. 2019, 19, 143–147.
  77. Laela, N.; Mohamad, A.; Fulyani, F. The effect of kefir-spirulina on glycemic status and antioxidant activity in hyperglycemia rats. Slovak J. Food Sci. 2021, 15, 101–110.
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