The two main carbohydrate polymers that can be found in the composition of LC biomass are, cellulose and hemicellulose, and lignin, but also other elements found in smaller percentages like other carbohydrates, ash, pectin, and proteins. Given the fact that lignocellulosic material is the principal component of plant cell walls, it is considered an abundant organic source in the world. The average amount of cellulose, hemicellulose, and lignin in most LC biomass is 30 to 60% cellulose, 20 to 40% hemicellulose, and 15 to 25% lignin, but these percentages vary for different materials [12].

There are also studies that give different percentage intervals for lignocellulose biomass composition, such as hemicellulose 10–40%, lignin 5–25%, and cellulose 40–80% [13].

As part of the lignocellulosic material, cellulose is a polymer, type polysaccharide of glucose disaccharide units, with cellobiose tightly connected by b-1, 4-glycoside bonds [14]. Cellulose is also the principal compound of plant cell walls, making it an abundant source of organic compound on earth [15]. Hydrogen bons link the molecules of cellulose with different orientations, leading to diverse levels of crystallinity, which has an important part in the biodegradation of cellulose, with a higher crystallinity leading to problems in the process [7].

Hemicellulose is branched heterogeneous polymer of diverse polysaccharides like pentoses, hexoses, and sugar acids [14]. Given its branched nature, it allows the formation of almost indestructible bonds with cellulose and lignin, increasing lignocellulosic material rigidity [16].

The second abundant compound of LC biomass on earth is lignin. Lignin is made out of phenylpropane units. Lignin links cellulose and hemicellulose in order to form a solid three-dimensional structure of the plant cell wall [7].

Looking at the structure of lignocellulosic matter, we can see that a skeleton is formed out of cellulose and by hemicellulose and lignin, similar to a matrix, and binding materials [17]. The high resistance of lignocellulose to biological degradation is linked to a high degree of refractory lignin presence [18]. An amorphous matrix material is formed by hemicellulose since it is non-covalently bonded to the external area of the surface of cellulose fibrils [19]. Even though it is the compound that has the weakest bond compound and it has a high chemical sensitivity, it plays a crucial role in strengthening the structure of lignocellulose. Lignin is a macromolecule that is crosslinked and relatively hydrophobic and aromatic, but it has a high resistance to biological degradation [20]. Cellulose is hydrophilic, because of the existence of groups R-OH, and internal hydrogen bonds, but not highly soluble in water because its large size [21]. To express better the composition of the lignocellulosic matter by high lightening the structure we added data that it is presented in Table 1. The chemical composition is mentioned for each structure cellulose, hemicellulose and lignin. Thus, the table below presents the composition of different lignocellulosic substrates.

AD is a well known method for converting organic materials into bioenergy, and its effectiveness and sustainability have been demonstrated by different research papers and industrial investigations [41]. The AD process uses microorganisms to degrade organic material and convert it to biogas. Degradation of lignocellulosic matter is dependent on enzymes produced by microorganisms. AD is separated in four main sequential steps: hydrolysis, acidogenesis, acetogenesis, and methanogenesis [42].

Even though AD is a proven method of converting organic matter into biomethane, the result was not the same in the case of LC biomass rigidity [16], due to the fact that LC biomass properties and composition inhibits the property of microorganisms and enzymes involved in AD to decompose the organicmaterial. This is because of lignin, which is like a rigid cover for cellulose and hemicellulose [43]. Multiple research papers have outlined the fact that greater lignin content in the organic fraction results in reduced biogas and methane yield [44]. Therefore, in order to have a productive AD process on lignocellulosic material, high in lignin content, different pretreatment steps are needed to expedite the hydrolysis of LC biomass, enhancing the biogas production.

To better illustrate the way LC biomass is structured, we can observe Figure 1. The molecules for lignin, cellulose, and hemicellulose are presented, as well as the three lignin monomers, coniferyl (G) alcohols, coumaryl (H) and sinapyl (S), and, with a detailed image of the crystalline cellulose.

Using enzymes in the LC biomass pretreatment is a common way to increase biogas yield, but its effect depends on the enzymes being used, and also the structure of the treated biomass, given enzyme specificity [47]. As an example, there is an enzyme called laccase, derived from phenoloxidase, which works by catalyzing phenol, anilines, and aromatic thiols oxidation in the feedstock, leading to microbial growth and fermentation ability being enhanced in the AD [48].

Efficiency of different enzymes for the AD process depend on several factors, including substrate composition, incubationperiod, temperature, pH levels, and reactor design [49].

Barley shell, pearl millet husk, rice straw, wheat bran, and wheat straw where subjected to a xylanases enzyme that was synthesized from Aspergillus niger. The results reported a saccharification of approximately 34.5% for rice straw after 8 h of incubation [50].

BP uses a variety of microorganisms and enzymes to selectively degrade lignin and hemicelluloses, resulting in enhanced biomass saccharification and biomass yield [51]. The best microorganisms for BP are the species of white-rot fungi from the Basidiomycetes class. Phanerochaete chrysosporium gives maximum efficiency due to its high growth rate and lignin degradation capabilities [52]. Microorganisms from the natural environment (Mcons), like soil, cow dung, goat dung, etc., are used for pretreating lignocellulosic material, having the ability to degrade both cellulose and hemicellulose.

Zhang et al. [53] created thermophilic Mcons from different sources, such as thermophilic landfill and decaying straw. This mixture was mixed with distillery wastewater in order to pretreat cassava waste at 55 °C for 12 h, resulting in a 96% higher methane yield.

Most pretreatment methods for lignocellulosic AD processes require high amounts of energy and chemical input in order to properly work, but they also create detrimental impacts [54]. Compared to these methods, BP is highly economical regarding energy and chemical requirements, and also does not produce inhibitory byproducts. BP is realized by the external addition of microorganisms and/or industrial and lignolytic enzymes and cellulase, to break down the lignocellulosic components [55]. Due to the fact that microbial enzymes are efficient in degrading lignocellulosic compounds, BP is known as one of the best ways of degrading this type of biomass, even though the enzymatic reaction rate is very slow [56].