Explore Bacterial Toxin Entry Computationally: History
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Subjects: Toxicology
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Many bacteria secrete toxic protein complexes that modify and disrupt essential processes in the infected cell that can lead to cell death. To conduct their action, these toxins often need to cross the cell membrane and reach a specific substrate inside the cell. The investigation of these protein complexes is essential not only for understanding their biological functions but also for the rational design of targeted drug delivery vehicles that must navigate across the cell membrane to deliver their therapeutic payload. Despite the immense advances in experimental techniques, the investigations of the toxin entry mechanism have remained challenging. Computer simulations are robust complementary tools that allow for the exploration of biological processes in exceptional detail.

  • computational methods
  • molecular dynamics simulations
  • coarse-grained simulations
  • bacterial toxin
  • membrane remodeling

1. Introduction

An effective strategy for many bacterial pathogens to damage a target cell is the secretion of toxin proteins that can modify and disrupt essential cellular processes and lead to cell death. The investigations of these protein complexes and their mechanism of action have contributed significantly to advances in several fields of life science. For instance, important mechanisms in cell biology have been discovered by unraveling specific interactions of toxin proteins with their target cells that are also essential for biomedical applications. To perform their action, bacterial toxins often need to cross the cell membrane and reach a specific location inside the cell, which suggests their great potential for biotechnological and therapeutic applications such as targeting specific tissue, organelle, or toxin-inspired targeted drug delivery vehicles’ design [1]. Additionally, the navigating capacity of bacterial toxins through different barriers of the cell regions point to fascinating mechanisms that are of great interest for biophysicists and chemists.
A diverse range of experimental techniques have been used to obtain high-resolution atomistic structures, the identification of their cellular receptor and binding affinities and entry pathways, e.g., cryo-electron microscopy and fluorescence microscopy imaging techniques, and biochemical assays [2][3][4][5]. Considerable insight has been gained from such complementary experimental approaches to the extent that some toxins are now used as tools in cell biological experiments [6]. For instance, cholera toxin was initially used to “identify” raft domains in cell and model membranes [7], and it is now clear that these toxins themselves reorganize raft lipid [8][9]. Because of their importance, more insight into the mechanism of action of bacterial toxins is needed.
However, for several reasons, experiments alone are insufficient to fully map out toxin entry to cells, and computational modeling is increasingly important: (i) all experimental measurements are prone to some limitations and inaccuracy that can lead to the misinterpretation of the physical principles covering the system, e.g., many experimental investigations use labeled biomolecules, which can perturb the system significantly, leading to incorrect conclusions. Here, it is important to check the proposed physical and chemical mechanism derived from experiments against idealized theoretical model calculations. (ii) Computational models often reveal molecular or cooperative mechanisms which can be tested experimentally, i.e., the computational methods serve as direct input to the design and analysis of experiments. (iii) The actions of many bacterial toxins are complex with sequential and/or concomitant physical and chemical processes involving vastly different length and time scales. Simple biochemical models fail in describing such systems, while multiscale computational modeling helps in combining the processes. (iv) Structural aspects of some bacterial toxins have been subject to evolutionary convergence, e.g., the bacterial Shiga and cholera toxins have common structural motifs for the membrane binding toxin subunits to induce membrane bending and to drive glycolipid–lectin (GL-Lect) endocytosis, although they have no sequence similarity and use different glycolipid receptors [10][11]. This suggests that generic physical properties of the membrane are exploited and computer simulations are an excellent tool for uncovering them.

2. Computational Methods

Despite the colossal complexity of biological systems, they are still subject entirely to the laws of physics. Thus, in principle, one can study every biological system by performing ab initio calculations, i.e., quantum calculation, on their constituent particles. In practice, this is impossible. Using the most powerful computers and the best available numerical algorithm, the ab initio calculation of systems containing thousands of particles would take decades, yet most biological systems are composed of a much larger number of atoms. Nevertheless, to describe most biological processes, highly accurate computational methods can be constructed without the need for first-principle theories.
In the past few decades, many powerful computational methods have been developed for the description of biological structures and functions. A large number of biological phenomena are now accessible for computational analysis, ranging from biomolecular dynamics, enzymatic processes, and virus assembly to the structure of small organelles [12][13][14], making computer simulation techniques an indispensable tool. Additionally, they are used for biomolecular engineering and drug design [15][16][17][18], Nevertheless, all computational methods have their strength in specific ranges of time and length scales, while they are inapplicable outside these ranges. Therefore, for many biological processes, several of these methods must be put into play. Multiscale simulation techniques provide such routes of complementary methods where the cooperative behavior of biomolecular assembly can be linked to molecular features. The development of such methods carries a promise of a new level of sophistication in the computational analysis of highly complex cellular phenomena. Besides the application of multiscale simulation techniques in basic research of the cell, it can aid the rational design of new drugs and drug delivery systems in pharmaceutical sciences and accommodate new approaches for combating disease in medical sciences [19][20]. The problem of bacterial toxin entry into the cell is among the first successful application of multiscale simulation methods. For certain classes of bacterial toxins, cell entry is initiated by series of processes: the diffusion of the toxin particle, binding to a membrane receptor, the aggregation of toxin–receptor complexes on the membrane, and large-scale deformation and tubular invagination of the host membrane. Therefore, multiple simulation methods to cover important features of these processes are needed. In the following, we focus on three major computational methods that can well cover the full scale required for bacterial-toxin-related phenomena. These methods are all-atom molecular dynamics simulations, coarse-grained, and mesoscopic models that together can cover a length scale from atomic distance to cellular scale and a time scale up to second (Figure 1).
Figure 1. Computational simulation methods for exploring toxin entry into the host cell. (A) aaMD simulation of a single Shiga toxin bound to the POPC bilayer containing a fraction of Gb3 lipids. The binding of the toxin to Gb3 generates a small local membrane curvature and suppresses membrane fluctuations [21]. (B) CG simulations of two toxin particles bound to a membrane; (top) Martini model where chemical moieties are retained; (bottom) DPD model where a still simpler forcefield is employed [22]. (C) Mesoscopic simulations of hundreds of toxin particles remodeling the membrane shape (picture is obtained from ref. [13]). (D) Back mapping structure from “C” to CG model for investigations with molecular detail (picture is obtained from ref. [13]).

3. All-Atom Molecular Dynamics Simulations

The all-atom molecular dynamics simulation (aaMD) is a unique method to investigate biomolecular systems at atomistic resolutions [14]. After many decades of work on the development of advanced and optimized simulation software packages and accurate force fields, aaMD has become an indispensable tool to capture biomolecular dynamics in the range of 10s of nm and the microsecond timescale [23][24][25][26].
To investigate the mechanism of bacterial toxins’ entry into the host cell and their mechanism of action, aaMD is highly important to explore the dynamics, conformation, and interactions of a single toxin particle and its cellular receptor [27][28][29]. For instance, the dynamics and stability of diphtheria toxin translocation T domain in neutral and low pH and in solution and in the vicinity of anionic membranes has been investigated thoroughly using aaMD [30][31][32][33]. Additionally, the conformation and phase behavior of globotriaosylceramide (Gb3) and monosialotetrahexosylganglioside (GM1), which, respectively, are the cellular receptors of Shiga and cholera toxins, has been explored using aaMD [29][34][35][36]. These studies unraveled several interesting behaviors, suggesting that the length and saturation level of the acyl chain and the composition of the lipid bilayers strongly affects toxic binding and activity. Furthermore, aaMD suggests that CTxB binds efficiently and strongly to lipid bilayers containing GM1 due to the significant amount of direct and water-mediated hydrogen bonds [27][34], supporting the crystal structure data [37].
Another interesting application of MD simulation is to check the reliability of molecular probes that are used for in vitro experiments of toxin behaviors. For instance, Rissanen et al. [34] used aaMD to explore the binding affinity of cholera toxin B-subunit (CTxB) to GM1 and acyl-chain labeled bodipy-GM1 (bdGM1). Interestingly, they found that bdGM1 shows reduced receptor availability in lipid bilayer mixtures, suggesting that the usage of bdGM1 to probe GM1 in cells can lead to an invalid interpretation of experimental data (Figure 2A).
Figure 2. aaMD simulation of a cholera toxin bound to a bilayer. (A) the dependence of GM1-CTxB binding on membrane environment in simulations of systems with CTxB (picture is made from ref. [34]); (B) CTxB induce local membrane curvature upon binding to a lipid bilayer containing a fraction of GM1 (picture is made from ref. [27]). Lo and Ld refer to liquid order and disorder phases, respectively.
To enter the host cell, many toxins generate their own endocytic pits through cooperative actions initiated by binding to their cellular receptor on the targeted cell membrane [38]. To do so, individual toxic particles need to modify and manipulate the host cell membrane structure. This is characterized by their local membrane curvature imprint, which measures how much an externally bound object deforms a membrane around it (it is the inverse of the induced radius of curvature). To understand how and to what extent proteins or nanoparticles induce a local curvature, aaMD is an effective and unique approach. Pezeshkian et al. used aaMD to investigate the curvature-inducing capacity of the B subunit of Shiga (STxB) and cholera toxin (CTxB) [21][27]. They found that STxB induces a curvature of 0.07 nm−1 (corresponding to 0.035 nm−1 mean curvature), while for CTxB, this value is 0.05 nm−1 (Figure 1A and Figure 2B). It was also found that despite the different molecular designs of STxB and CTxB, the structure of a complex containing each toxin and its glycosphingolipid receptors, i.e., CTxB-5GM1 and STxB-15Gb3, induces a local curvature based on a similar principle. The combination of the positioning of specific binding units and protein shape provides a structural motif for curvature generation, which also has been termed the glycolipid–lectin (GL-Lect) hypothesis [10][11]. This new mechanism can be exploited for the design of drug delivery vehicles [21][27]. Interestingly, a recent experimental investigation using super-resolution and polarized localization microscopy on different CTxB mutants, each capable of binding to a different number of GM1, supports this picture and provides strong evidence that the CTxB-GM1 complex is the driver of the membrane curvature, not the CTxB alone [39][40]. It is worth noticing that both CTxB and STxB can enter a targeted cell by inducing long and narrow tubular membrane invaginations [5][41]. An important lesson from these studies is that the capacity of bacterial toxins to induce local membrane curvature within a specific range is crucial for their entry, and therefore provides a tuning parameter for the rational design of drug delivery vehicles. Curvature generation is also used to drive the endocytosis of endogenous proteins via the GL-Lect mechanism (Refs. [42][43][44], reviewed in [10]).
Overall, aaMD is a highly efficient and robust method to investigate toxin proteins and their interactions with the host membrane with an atomistic level of resolution. Nevertheless, there is a major limitation with aaMD: it is only applicable for dynamics at short times and on small length scales and is therefore only applicable to study single bacterial toxin particles and their interaction with a host membrane.

4. Coarse-Grain Simulations

To capture biomolecular dynamics and interactions beyond the scales reachable with aaMD, coarse-grained (CG) models are at play. In this approach, a group of atoms is represented by an effective interaction site that results in a reduced system size (fewer number of particles), the removal of the fast degrees of freedom (allowing the use of larger time steps in the integration of the equations of motion), and smoother energy landscapes (faster dynamics) [45][46]. Owing to these inherent characteristics, CG models have become a powerful computational technique for biomolecular investigations in the length and time scales up to 100s of nm and 100s of microsecond, respectively.
In the domain of toxin protein entry, these models are well suited to investigate the interactions between several toxic proteins, especially when they are bound to a biomembrane. For instance, the mechanism of STxB clustering on biomembranes has been explored using the dissipative particle dynamics method (a type of CG modeling) [22][47]. In this study, the authors ignore all the molecular details of STxB and represent it as a 7 nm diameter rigid pentagonal nanoparticle with an affinity to strongly bind to the biomembrane surface, a generic minimal model for rigid peripheral membrane proteins (Figure 1B). This simple model finds that the tight binding of sufficiently large nanoparticles is enough for them to cluster on the membrane surface, even in the absence of any direct interaction. The origin of the clustering mechanism is the ability of the nanoparticle to locally suppress membrane shape fluctuations; in response, the membrane generates an attractive force among these particles that drive them to cluster to increase the conformational entropy of the membrane, a mechanism which is termed the thermal Casimir-like force [48]. Interestingly, it is found experimentally that STxB clustering on membranes is strongly correlated to toxin tight binding to the membrane surface, and this clustering is necessary for the formation of tubular membrane invaginations, which is the initial step of STxB entry [22]. In another study, Reynwar et al. [49] used a solvent-free CG model [50] and showed that nanoparticles could aggregate (curvature mediated forces) and drive the membrane to vesiculate (a model for endocytic pit formation) when they induce large local membrane curvature. The importance of these results and methods is that they are generic and do not depend on a specific atomistic structure, and therefore provide design principles to learn from toxin proteins to engineer targeted drug delivery vehicles that can cross the cellular membrane effectively.
Another class of coarse-grained models aims to keep a certain degree of chemical specificity and atomistic accuracy while still benefiting from the speedups of CG modeling [51]. The forcefield of these methods is continually being optimized to make better predictions of realistic systems [52]. The Martini model, a popular CG model that maintains chemical specificity [53] (Figure 1B), has been used to investigate the effect of CTxB and its cellular receptor, i.e., GM1 on lipid bilayer phases and structures [54][55], and the binding of STxB and lectin I from Pseudomonas aeruginosa to membranes containing Gb3 [56]. Flores-Canales et al. [57] have created a CG model based on atomistic structure, to study the interactions of the diphtheria toxin translocation domain with biomembranes.
Overall, CG models are versatile tools to investigate the mechanism of bacterial toxin entry into the host cell. On the one hand, they can be used as a simplified model to obtain generic physical features for the design of synthetic drug delivery vehicles; on the other hand, they can be exploited to investigate toxic-related biological mechanisms at near atomistic resolution.

5. Summary 

Computer modeling techniques have become an indispensable investigatory tool for exploring biological processes on a wide range of time and length scales. Owing to the rapid technological progress in hardware and software, and efficient algorithms and force fields, they have emerged as a new field, filling the gap between the theoretical and experimental investigations. Indeed, the mechanism of action of bacterial toxins is a good example. In particular, with the development of new multiscale simulation approaches, valuable information about their mechanism of action and entry pathway has been obtained. Multiscale simulations provide a scheme for analyzing the cascade of successive complex processes of toxin action from the molecular details to the effect of membrane structural deformations and fluctuations. With the fast progress in the multiscale simulation techniques, these methods will become a standard tool complementary to experiments in the research of toxins. Additionally, simulations can provide certain collective properties emerging from molecular features that can be used as tuning parameters in the rational design and engineering of drug delivery vehicles.

This entry is adapted from the peer-reviewed paper 10.3390/toxins13070449

References

  1. Schiavo, G.; Van Der Goot, F.G. The bacterial toxin toolkit. Nat. Rev. Mol. Cell Biol. 2001, 2, 530–537.
  2. Klenow, M.B.; Jeppesen, J.C.; Simonsen, A.C. Membrane rolling induced by bacterial toxins. Soft Matter 2020, 16, 1614–1626.
  3. Piper, S.J.; Brillault, L.; Rothnagel, R.; I Croll, T.; Box, J.K.; Chassagnon, I.; Scherer, S.; Goldie, K.N.; A Jones, S.; Schepers, F.; et al. Cryo-EM structures of the pore-forming A subunit from the Yersinia entomophaga ABC toxin. Nat. Commun. 2019, 10, 1–12.
  4. Bacia, K.; Scherfeld, D.; Kahya, N.; Schwille, P. Fluorescence Correlation Spectroscopy Relates Rafts in Model and Native Membranes. Biophys. J. 2004, 87, 1034–1043.
  5. Römer, W.; Berland, L.; Chambon, V.; Gaus, K.; Windschiegl, B.; Tenza, D.; Aly, M.R.E.; Fraisier, V.; Florent, J.-C.; Perrais, D.; et al. Shiga toxin induces tubular membrane invaginations for its uptake into cells. Nature 2007, 450, 670–675.
  6. Chang, K.J.; Benett, V.; Cuatrecasas, P. Membrane receptors as general markers for plasma membrane isolation procedures. The use of 125-I-labeled wheat germ agglutinin, insulin, and cholera toxin. J. Biol. Chem. 1975, 250, 488–500.
  7. Shogomori, H.; Futerman, A. Cholera Toxin Is Found in Detergent-insoluble Rafts/Domains at the Cell Surface of Hippocampal Neurons but Is Internalized via a Raft-independent Mechanism. J. Biol. Chem. 2001, 276, 9182–9188.
  8. Arumugam, S.; Schmieder, S.; Pezeshkian, W.; Becken, U.; Wunder, C.; Chinnapen, D.; Ipsen, J.H.; Kenworthy, A.K.; Lencer, W.; Mayor, S.; et al. Ceramide structure dictates glycosphingolipid nanodomain assembly and function. Nat. Commun. 2021, 12, 3675–3687.
  9. Hammond, A.T.; Heberle, F.; Baumgart, T.; Holowka, D.; Baird, B.; Feigenson, G.W. Crosslinking a lipid raft component triggers liquid ordered-liquid disordered phase separation in model plasma membranes. Proc. Natl. Acad. Sci. USA 2005, 102, 6320–6325.
  10. Johannes, L.; Wunder, C.; Shafaq-Zadah, M. Glycolipids and Lectins in Endocytic Uptake Processes. J. Mol. Biol. 2016, 428, 4792–4818.
  11. Johannes, L. Shiga Toxin—A Model for Glycolipid-Dependent and Lectin-Driven Endocytosis. Toxins 2017, 9, 340.
  12. Doosti, B.A.; Pezeshkian, W.; Bruhn, D.S.; Ipsen, J.; Khandelia, H.; Jeffries, G.D.M.; Lobovkina, T. Membrane Tubulation in Lipid Vesicles Triggered by the Local Application of Calcium Ions. Langmuir 2017, 33, 11010–11017.
  13. Pezeshkian, W.; Marrink, S.J. Simulating realistic membrane shapes. Curr. Opin. Cell Biol. 2021, 71, 103–111.
  14. Enkavi, G.; Javanainen, M.; Kulig, W.; Róg, T.; Vattulainen, I. Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance. Chem. Rev. 2019, 119, 5607–5774.
  15. Joshi, H.; Bhatia, D.; Krishnan, Y.; Maiti, P.K. Probing the structure and in silico stability of cargo loaded DNA icosahedra using MD simulations. Nanoscale 2017, 9, 4467–4477.
  16. Patmanidis, I.; De Vries, A.H.; Wassenaar, T.A.; Wang, W.; Portale, G.; Marrink, S.J. Structural characterization of supramolecular hollow nanotubes with atomistic simulations and SAXS. Phys. Chem. Chem. Phys. 2020, 22, 21083–21093.
  17. Khan, F.I.; Wei, D.; Gu, K.-R.; Hassan, I.; Tabrez, S. Current updates on computer aided protein modeling and designing. Int. J. Biol. Macromol. 2016, 85, 48–62.
  18. Souza, P.C.T.; Thallmair, S.; Conflitti, P.; Ramírez-Palacios, C.; Alessandri, R.; Raniolo, S.; Limongelli, V.; Marrink, S.J. Protein–ligand binding with the coarse-grained Martini model. Nat. Commun. 2020, 11, 1–11.
  19. Boye, T.L.; Maeda, K.; Pezeshkian, W.; Sønder, S.L.; Haeger, S.C.; Gerke, V.; Simonsen, A.C.; Nylandsted, J. Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair. Nat. Commun. 2017, 8, 1623–1634.
  20. Florentsen, C.D.; Kamp-Sonne, A.; Moreno-Pescador, G.; Pezeshkian, W.; Zanjani, A.A.H.; Khandelia, H.; Nylandsted, J.; Bendix, P.M. Annexin A4 trimers are recruited by high membrane curvatures in giant plasma membrane vesicles. Soft Matter 2021, 17, 308–318.
  21. Pezeshkian, W.; Hansen, A.G.; Johannes, L.; Khandelia, H.; Shillcock, J.; Kumar, P.B.S.; Ipsen, J.H. Membrane Invagination Induced by Shiga toxin B-subunit: From Molecular Structure to Tube Formation. Soft Matter 2016, 12, 5164–5171.
  22. Pezeshkian, W.; Gao, H.; Arumugam, S.; Becken, U.; Bassereau, P.; Florent, J.-C.; Ipsen, J.H.; Johannes, L.; Shillcock, J.C. Mechanism of Shiga Toxin Clustering on Membranes. ACS Nano 2017, 11, 314–324.
  23. Huang, J.; Rauscher, S.; Nawrocki, G.; Ran, T.; Feig, M.; de Groot, B.L.; Grubmuller, H.; MacKerell, A.D., Jr. CHARMM36m: An improved force field for folded and intrinsically disordered proteins. Nat. Methods 2017, 14, 71–73.
  24. Phillips, J.C.; Hardy, D.J.; Maia, J.D.C.; Stone, J.E.; Ribeiro, J.V.; Bernardi, R.C.; Buch, R.; Fiorin, G.; Hénin, J.; Jiang, W.; et al. Scalable molecular dynamics on CPU and GPU architectures with NAMD. J. Chem. Phys. 2020, 153, 044130.
  25. Abraham, M.J.; Murtola, T.; Schulz, R.; Páll, S.; Smith, J.; Hess, B.; Lindahl, E. GROMACS: High performance molecular simulations through multi-level parallelism from laptops to supercomputers. SoftwareX 2015, 1–2, 19–25.
  26. Wang, J.; Wolf, R.M.; Caldwell, J.W.; Kollman, P.A.; Case, D.A. Development and testing of a general amber force field. J. Comput. Chem. 2004, 25, 1157–1174.
  27. Pezeshkian, W.; Nåbo, L.J.; Ipsen, J.H. Cholera toxin B subunit induces local curvature on lipid bilayers. FEBS Open Bio 2017, 7, 1638–1645.
  28. Basu, I.; Mukhopadhyay, C. Insights into Binding of Cholera Toxin to GM1 Containing Membrane. Langmuir 2014, 30, 15244–15252.
  29. Pezeshkian, W.; Chaban, V.V.; Johannes, L.; Shillcock, J.; Ipsen, J.; Khandelia, H. The effects of globotriaosylceramide tail saturation level on bilayer phases. Soft Matter 2015, 11, 1352–1361.
  30. Flores-Canales, J.C.; Kurnikova, M.; Maria, K. Microsecond Simulations of the Diphtheria Toxin Translocation Domain in Association with Anionic Lipid Bilayers. J. Phys. Chem. B 2015, 119, 12074–12085.
  31. Ghatak, C.; Rodnin, M.V.; Vargas-Uribe, M.; McCluskey, A.J.; Flores-Canales, J.C.; Kurnikova, M.; Ladokhin, A.S. Role of Acidic Residues in Helices TH8–TH9 in Membrane Interactions of the Diphtheria Toxin T Domain. Toxins 2015, 7, 1303–1323.
  32. Flores-Canales, J.C.; Simakov, N.A.; Kurnikova, M. Microsecond Molecular Dynamics Simulations of Diphtheria Toxin Translocation T-Domain pH-Dependent Unfolding in Solution. bioRxiv 2019, 572040.
  33. Ladokhin, A.S. pH-triggered conformational switching along the membrane insertion pathway of the diphtheria toxin T-domain. Toxins 2013, 5, 1362–1380.
  34. Rissanen, S.; Grzybek, M.; Orłowski, A.; Róg, T.; Cramariuc, O.; Levental, I.; Eggeling, C.; Sezgin, E.; Vattulainen, I. Phase Partitioning of GM1 and Its Bodipy-Labeled Analog Determine Their Different Binding to Cholera Toxin. Front. Physiol. 2017, 8, 252.
  35. Lingwood, D.; Binnington, B.; Róg, T.; Vattulainen, I.; Grzybek, M.; Ünal, C.; Lingwood, C.A.; Simons, K. Cholesterol modulates glycolipid conformation and receptor activity. Nat. Chem. Biol. 2011, 7, 260–262.
  36. Manna, M.; Javanainen, M.; Monne, H.M.-S.; Gabius, H.-J.; Rog, T.; Vattulainen, I. Long-chain GM1 gangliosides alter transmembrane domain registration through interdigitation. Biochim. Biophys. Acta (BBA) Biomembr. 2017, 1859, 870–878.
  37. Merritt, E.A.; Kuhn, P.; Sarfaty, S.; Erbe, J.L.; Holmes, R.K.; Hol, W.G.J. The 1.25 Å resolution refinement of the cholera toxin B-pentamer: Evidence of peptide backbone strain at the receptor-binding site. J. Mol. Biol. 1998, 282, 1043–1059.
  38. Johannes, L.; Parton, R.; Bassereau, P.; Mayor, S. Building endocytic pits without clathrin. Nat. Rev. Mol. Cell Biol. 2015, 16, 311–321.
  39. Kabbani, A.M.; Raghunathan, K.; Lencer, W.I.; Kenworthy, A.K.; Kelly, C.V. Structured clustering of the glycosphingolipid GM1 is required for membrane curvature induced by cholera toxin. Proc. Natl. Acad. Sci. USA 2020, 117, 14978–14986.
  40. Groza, R.; Ewers, H. Membrane deformation by the cholera toxin beta subunit requires more than one binding site. Proc. Natl. Acad. Sci. USA 2020, 117, 17467–17469.
  41. Ewers, H.; Römer, W.; Smith, A.E.; Bacia, K.; Dmitrieff, S.; Chai, W.; Mancini, R.; Kartenbeck, J.; Chambon, V.; Berland, L.; et al. GM1 structure determines SV40-induced membrane invagination and infection. Nat. Cell Biol. 2009, 12, 11–18.
  42. Ivashenka, A.; Wunder, C.; Chambon, V.; Sandhoff, R.; Jennemann, R.; Dransart, E.; Podsypanina, K.; Lombard, B.; Loew, D.; Lamaze, C.; et al. Glycolipid-dependent and lectin-driven transcytosis in mouse enterocytes. Commun. Biol. 2021, 4, 1–15.
  43. Lakshminarayan, R.; Wunder, C.; Becken, U.; Howes, M.; Benzing, C.; Arumugam, S.; Sales, S.; Ariotti, N.; Chambon, V.; Lamaze, C.; et al. Galectin-3 drives glycosphingolipid-dependent biogenesis of clathrin-independent carriers. Nat. Cell Biol. 2014, 16, 592–603.
  44. Renard, H.-F.; Tyckaert, F.; Giudice, C.L.; Hirsch, T.; Valades-Cruz, C.A.; Lemaigre, C.; Shafaq-Zadah, M.; Wunder, C.; Wattiez, R.; Johannes, L.; et al. Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166. Nat. Commun. 2020, 11, 1–13.
  45. Ingólfsson, H.I.; Lopez, C.A.; Uusitalo, J.J.; De Jong, D.H.; Gopal, S.M.; Periole, X.; Marrink, S.J. The power of coarse graining in biomolecular simulations. Wiley Interdiscip. Rev. Comput. Mol. Sci. 2014, 4, 225–248.
  46. Saunders, M.G.; Voth, G.A. Coarse-Graining Methods for Computational Biology. Annu. Rev. Biophys. 2013, 42, 73–93.
  47. Lipowsky, R.; Brinkmann, M.; Dimova, R.; Haluska, C.; Kierfeld, J.; Shillcock, J. Wetting, budding, and fusion—morphological transitions of soft surfaces. J. Phys. Condens. Matter 2005, 17, S2885–S2902.
  48. Johannes, L.; Pezeshkian, W.; Ipsen, J.H.; Shillcock, J.C. Clustering on Membranes: Fluctuations and More. Trends Cell Biol. 2018, 28, 405–415.
  49. Reynwar, B.J.; Illya, G.; Harmandaris, V.A.; Müller, M.M.; Kremer, K.; Deserno, M. Aggregation and vesiculation of membrane proteins by curvature-mediated interactions. Nature 2007, 447, 461–464.
  50. Cooke, I.; Kremer, K.; Deserno, M. Tunable generic model for fluid bilayer membranes. Phys. Rev. E 2005, 72 Pt 1, 011506.
  51. Kmiecik, S.; Gront, D.; Kolinski, M.; Wieteska, L.; Badaczewska-Dawid, A.E.; Kolinski, A. Coarse-Grained Protein Models and Their Applications. Chem. Rev. 2016, 116, 7898–7936.
  52. Souza, P.C.T.; Alessandri, R.; Barnoud, J.; Thallmair, S.; Faustino, I.; Grünewald, F.; Patmanidis, I.; Abdizadeh, H.; Bruininks, B.M.H.; Wassenaar, T.A.; et al. Martini 3: A general purpose force field for coarse-grained molecular dynamics. Nat. Methods 2021, 18, 382–388.
  53. Marrink, S.J.; Risselada, H.J.; Yefimov, S.; Tieleman, D.P.; de Vries, A.H. The MARTINI force field: Coarse grained model for biomolecular simulations. J. Phys. Chem. B 2007, 111, 7812–7824.
  54. Liu, Y.; Barnoud, J.; Marrink, S.J. Gangliosides Destabilize Lipid Phase Separation in Multicomponent Membranes. Biophys. J. 2019, 117, 1215–1223.
  55. Sridhar, A.; Kumar, A.; DasMahapatra, A.K. Multi-scale molecular dynamics study of cholera pentamer binding to a GM1-phospholipid membrane. J. Mol. Graph. Model. 2016, 68, 236–251.
  56. Kociurzynski, R.; Makshakova, O.N.; Knecht, V.; Römer, W. Multiscale Molecular Dynamics Studies Reveal Different Modes of Receptor Clustering by Gb3-Binding Lectins. J. Chem. Theory Comput. 2021, 17, 2488–2501.
  57. Flores-Canales, J.C.; Vargas-Uribe, M.; Ladokhin, A.S.; Kurnikova, M. Membrane Association of the Diphtheria Toxin Translocation Domain Studied by Coarse-Grained Simulations and Experiment. J. Membr. Biol. 2015, 248, 529–543.
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