Recombinant enzymes are catalytically active proteins produced through expression of engineered DNA that encodes the enzyme in a biological host. The coding sequence is incorporated into an expression construct whose regulatory elements support transcription and translation in the selected organism. The encoded protein may retain a naturally occurring sequence or contain deliberately introduced sequence changes [1]. Formation of an active recombinant enzyme can require folding, assembly of subunits, incorporation of cofactors, proteolytic processing, or other post-translational modifications [2][3]. These requirements interact with the biochemical capabilities of the expression host [2]. The enzyme may accumulate within cells or enter a secretion pathway, and its mature molecular form depends on both the encoded sequence and host-mediated processing [4][4]. The term identifies the recombinant origin of the enzyme and does not specify a single catalytic reaction, protein family, or expression organism [1][2].
- recombinant expression
- catalytic proteins
- expression host
- protein maturation
🔵 Enzyme Production and Characterization • 🟣 Biotechnology • 🟡 Biochemistry, Genetics and Molecular Biology • 🔴 Life Sciences
References
- Marie Deckers; Dieter Deforce; Marie-Alice Fraiture; Nancy H.C. Roosens; Genetically Modified Micro-Organisms for Industrial Food Enzyme Production: An Overview. Foods 2020, 9, 326, 10.3390/foods9030326.
- Antonio Milton Vieira Gomes; Talita Souza Carmo; Lucas Silva Carvalho; Frederico Mendonça Bahia; Nádia Skorupa Parachin; Comparison of Yeasts as Hosts for Recombinant Protein Production. Microorganisms 2018, 6, 38, 10.3390/microorganisms6020038.
- Dujuan Liu; Sandra Garrigues; Ronald P. de Vries; Heterologous Protein Production in Filamentous Fungi. Appl. Microbiol. Biotechnol. 2023, 107, 5019-5033, 10.1007/s00253-023-12660-8.
- Chun Liu; Yaping Zhang; Liu Yang; Fengguang Zhao; Shuangyan Han; Reinforcement Strategies of Chassis Cell Secretory Pathway for Improving Heterologous Protein Production. Microbe 2024, 3, 100092, 10.1016/j.microb.2024.100092.