Your browser does not fully support modern features. Please upgrade for a smoother experience.
Fourier Transform Mass Spectrometry: History
Please note this is an old version of this entry, which may differ significantly from the current revision.
Contributor: Helena Kang

Time-domain image-current or related ion signals that are converted by Fourier transformation into a frequency-domain mass spectrum constitute Fourier transform mass spectrometry (FTMS). The frequency of ion motion—cyclotron frequency in an ICR cell or axial oscillation frequency in an electrostatic (Orbitrap) trap—is a precise function of mass-to-charge ratio, so that long, coherent transients yield high resolving power [1]. Space charge shifts those frequencies and must be accounted for in mass calibration [2]. Collisional or electron-based activation of trapped multiply charged ions can be performed inside the same analyzer, linking the FTMS measurement to tandem structural analysis [3]. Conceptual boundaries exclude scanning beam instruments (magnetic sector, quadrupole) and time-of-flight analyzers that do not record a Fourier-transformed transient, and they include both FT-ICR and Orbitrap implementations as members of the FTMS class [4]. Essential features are ion trapping that supports periodic motion, broadband detection of that motion, and a Fourier-transform conversion from time to mass-to-charge ratio.

  • Fourier transform mass spectrometry
  • FTMS
  • ion trap
  • mass-to-charge ratio

Metabolomics and Mass Spectrometry Studies • Molecular Biology • Biochemistry, Genetics and Molecular Biology • Life Sciences

References

  1. Michaela Scigelova; Martin Hornshaw; Anastassios Giannakopulos; Alexander Makarov; Fourier Transform Mass Spectrometry. Mol. Cell. Proteom. 2011, 10, M111.009431, 10.1074/mcp.m111.009431.
  2. Edward B. Ledford; Don L. Rempel; M. L. Gross; Space charge effects in Fourier transform mass spectrometry. II. Mass calibration. Anal. Chem. 1984, 56, 2744-2748, 10.1021/ac00278a027.
  3. Michael W. Senko; J. Paul. Speir; Fred W. McLafferty; Collisional Activation of Large Multiply Charged Ions Using Fourier Transform Mass Spectrometry. Anal. Chem. 1994, 66, 2801-2808, 10.1021/ac00090a003.
  4. Luca Fornelli; Daniel Ayoub; Konstantin Aizikov; Alain Beck; Yury O. Tsybin; Middle-Down Analysis of Monoclonal Antibodies with Electron Transfer Dissociation Orbitrap Fourier Transform Mass Spectrometry. Anal. Chem. 2014, 86, 3005-3012, 10.1021/ac4036857.
More
This entry is offline, you can click here to edit this entry!
Write an Entry