The cultivation of potential antibiotic resistant
Salmonella colonies from human fecal and food samples involves five stages: pre-enrichment incubation, enrichment, plating, screening, and confirmation [
4]. Pre-enrichment incubation (24 h) employs non-selective (35 °C) media such as peptone water and lactose broth to expedite the recovery of sub-level injured
Salmonella. Although for resistant
Salmonella and cells that have entered the viable but non-culturable (VBNC) state, an antibiotic or selenite broth may be preferred. The pre-enriched media is then inoculated into selective media containing inhibitory bile salts such as thiosulphate malachite green, thiosulphate, sulphamethazine or novobiocin promoting the growth of
Salmonella over other bacteria [
23,
24]. Alternatively, official (Bacteriological Analytical manual (BAM)) inhibitory media such as Rappaport–Vassiliadis (RV) or tetrathionate broth can be employed. After sufficient enrichment (10
4 cells mL
−1), cells from the chosen media are plated onto selective agars. Commonly used agars include xylose-lysine-deoxycholate agar (XLD), brilliant green agar (BGA),
Salmonella–Shigella agar (SS), bismuth-sulfite agar (BSA) and Hektoen enteric (HE). Colonies from different serotypes present in various colors black (
S. Typhi) or pink (
S. Arizonae), depending on the agar medium. Occasionally, serotypes (
S. Montevideo) are not distinctive and are even missed (Lac+
Salmonella enterica Virchow,
S. enterica Newport
or S. enterica Typhi) and cannot be reliably identified at this stage of the process. After plating, suspected
Salmonella colonies undergo incubation in/on a group specific media such as a triple sugar iron (TSI) or lysine iron agar (LIA) slant [
23] followed by a confirmatory test for urease negative cultures. Cultures giving typical
Salmonella reactions are then selected for further biochemical and serological testing. However, the same serotype can vary with antigenicity. In such cases a polyvalent
Salmonella O antisera or a polyvalent
Salmonella bacteriophage O1-OE serological confirmatory test maybe also be employed [
6,
23,
24]. It should be noted that antimicrobial resistant
Salmonella spp. can be recovered by incorporating antibiotics in pre-enrichment and selective media [
25].
3. Automated Whole Genome Sequencing
Whole genome sequencing (WGS) is a powerful, inexpensive open access epidemiological tool that can predict the genotypic and phenotypic resistance of a suspected bacterium in just a few days. Multi-locus sequence typing (MLST), multiple-locus variable number of tandem repeats analysis (MLVA), single-nucleotide polymorphism (SNP) analysis, [
43], CRISPR-multi-locus virulence sequence typing (CRISPR-MVLST) [
44] and next-generation sequencing (NGS) are some of the techniques [
45] used in the sequencing of NTS antibiotic-resistant gene clusters.
Upon acquisition, sequences are then compared to reference sets sourced from databases such as (ResFinder [Center for Genomic Epidemiology, DTU]), BLAST (blastn) and ARG-ANNOT, followed by a phenotypic test to validate the predicted accuracy. Typically, an isolate will be genotypically resistant if a suspected antimicrobial resistant (AMR) gene is 75% identical to a reference sequence. Mismatches naturally arise, resulting in errors; Broadly, there are two mismatch categories, very major errors (VME) and major errors (ME). VME’s occur when a microbial sequence is predicted to be genotypically susceptible yet is phenotypically resistant. Conversely, major errors arise when a microbe identified as genetically resistant expresses phenotypic susceptibility [
46].
ME discrepancies seem to be associated with the breakpoints used for phenotypic testing. In some cases, the minimum inhibitory concentrations (MIC) are just below the recommended breakpoints suggesting technical variations in the agar dilution method may result in isolates being falsely classified as susceptible. Moreover, some plasmids encoding for antimicrobial resistance genes can be damaged during storage and sub-culturing, further affecting the ME rate. In the VME category, mismatches are generally attributed to the presence of resistance determinants absent in the reference database or novel resistance mechanisms whose genetic determinants have yet to be determined.
These mismatches seem to be a relevant issue [
47], especially when predicting streptomycin resistance. Despite these issues, ME and VME rates from numerous antibiotic resistant
Salmonella WGS studies remain below the acceptable FDA cut-offs of 3 and 1.5%. In an extensive WGS study [
48] involving 640 NTS Salmonellae, the susceptibility of 43 different serotypes to 14 antimicrobials was tested. Using the phenotypic results as the reference outcome, the authors calculated sensitivities by dividing the number of isolates that were genotypically resistant by the total number of isolates exhibiting clinical resistance phenotypes. Specificity was calculated by dividing the number of isolates that were genotypically susceptible by the total number of isolates with susceptible phenotypes. A total of 65 unique resistance genes plus mutations in two structural resistance loci were identified. Minimal sensitivity and specificity values of 86.4 (sulfisoxazole) and 90.8% (streptomycin) were reported.
In another WGS study, AMR
Salmonella enterica serovars (Typhimurium, Newport, and Dublin) from 90 isolates sourced from humans and cattle were compared. Isolates were screened for phenotypic resistance to 12 antibiotics. Genotypic prediction of phenotypic resistance resulted in a mean sensitivity of 97.2 and specificity of 85.2, respectively [
49]. Additional work [
46] confirmed [
48] previous observations regarding discrepancies between phenotypic resistance and genotypic resistance of aminoglycoside resistant genes. The authors concluded that 35 isolates carrying streptomycin resistance genes were phenotypically susceptible to the drug.
In 2019, a WGS study [
50] involving multiple phenotypic susceptibility testing methods produced ME and VME rates of 4.5% and 17%. Fifty-one of the VME’s were the result of discordant sulfisoxazole and sulfamethoxazole predictions. Interpretation of seven antimicrobials using
Salmonella clinical breakpoints produced low genotyping sensitivity and specificities values of 0.84 and 0.88, respectively. Upon exclusion of the VME rates for streptomycin and sulfamethoxazole, said values increased to 0.89 and 0.97. Notably, the WGS-based genotyping methods used in the study did not account for attenuation mechanisms or reliably predict for underlying temporary genetic features (tandem repeats) present in subpopulations (heteroresistance) during phenotypic testing [
51].
Whether heteroresistance will prevent WGS from becoming the primary diagnostic tool for antibiotic resistant pathogens is debatable, given the techniques improving resolution and increasing usage by food and drug industries. It is worth mentioning that for the past thirty years, the evolution of MDR
S. enterica serotype Kentucky and the subsequent emergence of the MDR clone in Africa and the EU were successfully mapped using WGS [
52]. During that time, WGS has undoubtedly improved our understanding of serovar resistome profiles and the employment of smarter antibiotic resistance combat strategies.
4. Automated Phenotypic Testing
4.1. Manual and Semi-Automated Antimicrobial Susceptibility Test (AST)
AST is used to determine the MIC of an antimicrobial required to limit a pathogen’s growth in accordance with guidelines provided by the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EU- CAST in optimized media for a standardized period [
53]. Traditionally that media is a broth or agar in which microdilution tests or disk diffusion tests between 35−37 °C are conducted. Tests performed using liquid-based methods measure change in optical density whilst disk diffusion methods estimate the antibiotic inhibition zone on agar plates following 24 h incubation. The size of the zone is a direct measure of the susceptibility of the bacteria to an antibiotic and is inversely related to the minimum inhibitory concentration MIC). Agar and broth dilution are some of the earliest techniques used to measure MIC. The advantages of broth dilution are its reproducibility and cost-effectiveness. A modern version of the technique called microbroth dilution, is now commonly employed.
Minimum inhibitory values are also determined by several other methods such as paper diffusion, E-tests, Biolog
MT plate, turbidity and absorbance assays. Although relatively inexpensive many of these growth dependent methods are hindered by long incubation periods sometimes taking more than 16 h to complete [
54]. As a matter of urgency, the FDA recently approved five semi-automated systems for clinical use, including Phoenix™ (Becton Dickinson Diagnostic Systems,
https://www.bd.com/en-us, accessed on 1 February 2021), Sensititer ARIS 2X™ (Trek Diagnostic Systems,
https://www.thermofisher.com, accessed on 1 February 2021), WalkAway™ (Siemens Medical Solutions Diagnostics,
https://www.beckmancoulter.com, accessed on 1 February 2021), and the VITEK systems 2™,
https://www.biomerieux-usa.com/vitek-2, accessed on 1 February 2021). The VITEK system employs three sensing modalities, absorbance, fluorescence, and turbidity, to determine an antibiotic’s MIC. The average time of an MIC for an antibiotic using the said system is 8 h. Recent work [
55] showed that the performance (sensitivity and range) of the VITEK system could be significantly enhanced when a mono-sulfonated tetrazolium salt such as “EZMTT” is added to the growth medium. Alternatively, one can use the highly automated fluorescent-based DxM Micro Scan WalkAway™ system. The advantage of DxM is that each MicroScan Dried Gram-Negative (MSDGN) MIC panel is pre-packaged with a tailored growth medium, fluorogenic panel and an antibiotic. Hydrolysis of the fluorogenic panel correlates directly to enzymatic activity, permitting the inclusion of persistent
Salmonella in the MIC measurement [
56].
Isolation, identification, and AST procedures can take from 2–7 days, depending on the number of samples and availability of automated culturing equipment (ACE). For example, the “Walk Away” specimen processor unit (WASP™) and BDs Kiestra TLA™ or BACTEC MGIT™ can reliably detect antibiotic resistant
Salmonella in a much shorter period compared to manual approaches [
57]. In 2018 initial trials of the Accelerated PhenoTest BC™ (Accelerated Diagnostics) showed it was possible to combine the identification and AST stages in a single test [
58].
The PhenoTest utilizes a combination of fluorescence in situ hybridization (FISH) and electro-kinetic focusing to concentrate and identify bacteria. The concentrated cells then undergo analysis via automated dark field microscopy (ADFM). ADFM utilizes a series of stacked z-images to monitor changes in colony shape, surface area, and segmentation. The compiled image permits the differentiation of bacterial growth, death, and elongation. When combined with deep learning processes, the Accelerated PhenoTest BC™ and the recently introduced QMAC-dRAST™ (QuantaMatrix, Inc.; Seoul, Korea) can generate a MIC in less than 7 h. Unfortunately, the FDA recalled the Accelerated PhenoTest BC™ kit in late 2018 due to the high number of false positives [
58]. For readers seeking an in-depth perspective regarding commercial AST testing, multiple reviews are recommended [
54,
58].
4.2. Detection of Intracellular Resistance Salmonella Using Flow Cytometry
Flow cytometry (FC) is an optical technique that measures the fluorescent and scattering properties of a laser integrated single line of cells as they continuously flow by a detector or detectors [
59]. Differing flow rates can be used to study antibiotic-induced changes in cellular morphology, intracell heterogeneity, cell-to-cell interaction (e.g., quorum sensing), and in the analysis of subpopulations of persistent and moderately resistant strains of
Salmonella [
60]. As well as persistence, FC has also been used to measure the transfer ratios of the multi-antibiotic resistant plasmid pB10 in multiple strains of
Salmonella [
61] and reliably determine antibiotic susceptible gram-negative phenotypes albeit in the absence of mammalian cells [
62]. In addition to susceptibility, the re-potentiation of antibiotics in the presence of primary metabolites and subsequent elimination of persistent
Salmonella from macrophages was also evaluated [
63] with FC.
Elimination of persistent
Salmonella residing in the lymph nodes of animals and humans remains a costly and significant challenge. Some of the initial work in 2014 [
64] using FC, a mouse typhoid fever model and the single-cell growth reporter (DsRed S197T) showed that differential host nutrient supply contributed to the heterogeneity of
Salmonella subsets. Assessment of the antimicrobial tolerance of the various subsets showed overall eradication was delayed primarily by abundant moderately growing
Salmonella with partial tolerance.
In another study, GFP-expressing
S. Dublin (SD3246-GFP) was used to estimate the degree of intracellular infection in bovine-derived macrophages sourced from 3 and 28-day-old Friesian bull calves. Confirmation by gentamicin-protection assay showed
S. Dublin intracellular replication and survival were arrested after 6 h and up to 24 h, respectively [
65]. Interestingly, FC results revealed that the majority of infected cells expressed MHCII, CD40, CD80, CD86, CD11b and CD11c but did not express CD1 Further analysis revealed that infected MHCII+ macrophage-like cells expressed elevated levels of MHCII and CD40 compared to uninfected cells, which is in contrast to observations reported with murine models. The authors attributed these inconsistencies to unnatural target species used in previous studies [
66].
Given FC’s ability to simultaneously measure susceptible, intermediate, and resistant (SIR) phenotypes, several initiatives have been proposed by various national, European, and international bodies to promote the development of rapid FC based AST assays. Of note is the commercialized FASTvet assay (FASTinov
®) developed by the Fast-Bac European consortium. In a recent study, the kit-assay exceeded expectations demonstrating an AST turn-around time of 2 h for 13 antimicrobials [
67], which is significantly faster than dRAST and many other automated phenotypic and genotypic platforms described herein. However, the authors did note that further testing using whole blood samples was required for veterinary usage.
Flow cytometry remains a highly versatile measurement tool, capable of determining the susceptibility of pre-enriched bacterial isolates, the transfer ratios of antibiotic resistant plasmids in multiple strains of Salmonella and mammalian cells that harbor antibiotic resistant species. Moreover, with the advent of the FASTvet assay, FC has the potential to rapidly identify and monitor antibiotic resistance bacteria in food animals.
5. Emerging Biosensors
Depending on the application (microbiology or biological), many definitions of a biosensor have been reported in the literature [
68,
69]. According to the International Union of Pure and Applied Chemistry, a biosensor is an integrated receptor (protein or DNA) transducer device capable of providing specific quantitative or qualitative information via a biological recognition element (BRE). Simply put, when a specific analyte binds to a receptor, a transfer (mass, electrical, thermal, magnetic, or photonic energy) occurs between the two, which is detected via a prescribed transduction element (e.g., electrode pattern, optical waveguide, fiber, cantilever, etc.). The transduction element then relays the information to an integrated (small screen) or separate (human, laptop) monitoring component. A “device” is a single entity upon which binding, transfer, and monitoring occur. Biological recognition elements used in microbial sensing include antibodies, toxins, nucleic acids, whole cells, and biomimetic materials [
70,
71,
72].
A summary of the biosensors used in the detection of antibiotic resistant serotypes discussed herein is shown Table 1.
Table 1. Biosensors used in the detection of antibiotic-resistant Salmonella serotypes. SERS (surface enhanced Raman spectroscopy).
Salmonella Serotypes
|
Sensing Method
|
Sample Matrix
|
Analysis Time (min)
|
Detection Limit (CFU/mL)
|
Reference
|
S. Typhimurium DT104
|
SERS
|
Assay media
|
30
|
105
|
[71]
|
S. Typhimurium DT104
|
SERS
|
Assay media
|
15
|
105
|
[74]
|
S. Typhimurium
|
SERS
|
H2O & milk
|
120
|
20
|
[72]
|
S. enterica
|
Raman Spectroscopy
|
Urine
|
150
|
n/a
|
[77]
|
S. Enteritidis,
|
Fluorescence
|
Water, milk, and beef
|
30
|
2.0 × 102
|
[86]
|
S. Typhimurium DT104 and S. Typhi
|
SERS
|
Assay media
|
120
|
100
|
[87]
|