Cyanobacteria-Derived Biofuel for Sustainable Future: History
View Latest Version
Please note this is an old version of this entry, which may differ significantly from the current revision.
Contributor:
Kshetrimayum Birla Singh
,
Dr. Kaushalendra
,
Savita Verma
,
Rowland Lalnunpuii
,
Jay Prakash Rajan

Cyanobacteria are valuable sources of many novel bioactive compounds, such as lipids and natural dyes, with potential commercial implications. One of the advantages of cyanobacteria is that their biochemical constituents can be modified by altering the source of nutrients and growth conditions.

  • algal biomass
  • algal oil feedstocks
  • phytoremediation
  • bioresource

1. Introduction

In technical terms, fossil fuels include coal, oil, natural gas, and hydrocarbons. Humans have been using fossil fuels since time immemorial. However, since the debut of the Industrial Revolution in the 18th century, the use of fossil fuels, especially coal, has expanded beyond the imagination. After the formation of fossil fuels deep inside the crust millions of years ago, its use was sped up only a few hundred years back when one person named James Watt invented the steam engine. The extensive use of fossil fuels has altered the socio-economic status and has a negative impact on our natural environment. In the past few years, the reduction in the availability of fossil fuels has ignited the global scientific community to move toward renewable energy sources, which are sustainable and eco-friendly. Renewable energy sources include wind power, hydropower, hydroelectricity, nuclear energy, solar energy, geothermal energy, and biofuel.
During biofuel production, biomass is transformed into ethanol and biodiesel in a limited time span with the help of biological/nonbiological agents. One of the best biological agents is “microbial agents,” which have drawn the attention of researchers worldwide [1][2][3]. Although humans are in the very early stage of the Type I civilization, our ever-increasing population and its dependency on machines have created significant demands for energy to run our modern and fast-growing global economies in the present and future [4][5][6][7]. The demerits of fossil fuels are their limited presence beneath the Earth’s crust, the production of GHGs in the environment [8][9][10][11], and the increasing cost of refining procedures, which in turn create fluctuations in the global price of petroleum products. Under such a scenario, a new generation of biofuel may play a crucial role in decreasing our dependency on conventional fossil fuels as well as curtail the global production of greenhouse gases (GHGs) in the environment. Hence, researchers around the globe are investing their efforts into energy fuels being sustainable, renewable, and eco-friendly [12][13][14][15][16][17][18][19][20]. Among the various microbiota, cyanobacteria have the potential to become a promising device for the production of the next generation of biofuel. They have an outstanding reputation in the biosphere because of their key ability to fix nitrogen and carbon [21][22][23]. Only recently, scientists have developed advanced techniques to isolate and identify the valuable secondary metabolites that may be used in biotechnological applications to solve environmental issues. Cyanobacterial metabolism can be easily applied to technical innovations and, thus, is practically the most suitable candidate for the large-scale commercial production of biofuel [24][25][26][27][28].
The best part of cyanobacteria-driven biofuel production is the zero release of pollutants, whereas harmful emissions are one of the most severe drawbacks of conventional energy sources. Because of these unmatchable properties, cyanobacteria have emerged as the best microbial candidate for biofuel production [29][30][31]. Moreover, several cyano-metabolites are precursors for producing various hydrocarbons in biofuel. In addition, cyano-metabolites are used in wastewater treatment and microalgal biorefinery, collectively known as phytoremediation. Here, the major effect of nutrient stress derived from wastewater and its utilization in altering the metabolite contents in algal cells is discussed, stressing the cost-benefit ratios in algal industries. Furthermore, an overview of The latest research works to initiate a biochemical way to extract and transesterify cyanobacteria to produce valuable biofuel is included. Finally, the benefits of cyanobacteria as bioresources of chemically active and modified compounds and their use in biofuel will be discussed.
Cyanobacteria are considered the oldest living organisms, which have existed for 3.5 billion years and flourish in every possible niche on Earth, even in extreme habitats, such as high-salinity ponds, hot springs, and polar regions [32][33][34][35][36][37]. They are among the key players of ecosystems, along with fungi and protozoans, providing crucial services in primary production, decomposition, and nutrient cycling. They are good bioindicators in aquatic environments, especially in coastal and brackish water. They flourish in nutrient-rich wastewater in various morphological features, such as unicellular, filamentous, or colonial forms, forming a mat-like structure [38][39][40][41][42]. Despite being an efficient global sink for atmospheric carbon through the photosynthetic process and natural nitrogen fixation they are also utilized as bio-agents for aquatic pollutant removal [43][44][45][46]. Recently, it has been reported that cyanobacterial growth significantly ameliorates the negative effects of herbicides and pesticides [47] in aquatic and terrestrial ecosystems. Besides being rich sources of chemical metabolites, such as carbohydrates, amino acids, and lipids, cyanobacteria do foster other chemical compounds, such as pigments and anti-oxidants, that can cure several pathological conditions [48][49]. Furthermore, cyanobacterial metabolites, especially carbohydrates, can be utilized to produce valuable biochemical derived biopolymers, biofertilizers, biofuels, nutraceuticals, and enzymes [50][51]. Their extraordinary richness in biomolecular diversity makes them the most promising biofuel agents [52]. Different species of cyanobacteria can be utilized to produce different fuels, such as cellulosic ethanol, biodiesel, biogas, and hydrogen [53]. The high productivity of primary and secondary metabolites in cyanobacteria is due to their potential to assimilate nitrogen and phosphorus in bioactive format from the environment, producing sufficient biomass during a limited growth period, as compared to other land plants or crops [54]. Such a high turnover of biomass is utilized by the neutraceutical and biorefinery industries, and leftover biomass is used as a primary substrate for the production of biofuel. Cyanobacterial farming does not demand land for production and is quickly grown on the aquatic system, including seawater, wastewater, and fresh water. They are the potential source of third-generation feedstock, converting solar energy into green fuels. The appropriate selection of cyanobacterial species matching the local environment is a crucial step for maximizing biomass production for economic and commercial success. The success of suitable cyanobacterial species according to the local environment lies behind their balanced cell stoichiometry and optimization at minimum operational cost. Furthermore, wastewater is the cheapest and best growth medium for algal biomass production because of its high nutritional value [55]. Recent scientific reports also indicate that algae-based wastewater remediation can also produce biofuel at a commercial level. Because of the bioremediation property of algae, they are employed in wastewater treatment from industrial and domestic sources, including slaughterhouses, textile pharmaceuticals, and agro-industries [56][57]. The efficiency of biofuel depends on many physical and chemical properties, such as oxidation constant, cetane number, cold flow, flash point, cloud point, pour point, etc. [58]. While selecting the most suitable strain/species of cyanobacteria for commercial biofuel production, one must consider all these properties along with the environmental condition. Recently, Fremyella diplosiphon has been reported to have transesterification of lipids in biodiesel, increasing the cetane number and oxidation constant above the threshold standards of biofuel [59]. In addition, it increases the density, viscosity, plugging point of the iodine cold filter, and cloud and pour points above the lowest acceptable level. Some other cyanobacterial species, such as Cyanobium sp., Limnothrix sp., and Nostoc sp., have been tested and commercialized in biodiesel production. In particular, Limnothrix has been shown to provide the optimum lipid profile with an increased abundance of C16:0 [60]. Many filamentous cyanobacteria are reported to produce high-valued chemicals, such as limonene, farnesene, and linalool. After the extraction of high-valued compounds, the residual biomass may undergo biological fermentation or transesterification for biofuel production [61].

2. Cyanobacteria as Potential Feedstocks for Biofuel Production

For biofuel synthesis, feedstock selection is the most important factor determining the lipid content and contributes to three-quarters of the total biofuel production. Table 1 shows how biofuel has evolved from first-generation to fourth-generation fuel production under different processes. Feedstocks can be categorized into five different classes based on the origin of the biofuel.
Table 1. Evolution of biofuel from conventional to fourth generation biofuel.
Type Nature Merits/Demerits References
Conventional Energy Sources Wood and plant residues (solid fuel) Undergoes incomplete combustion; produces CO, CO2, SO2, NO2, and particulate material, which are injurious to health and environment [62]
First generation—Biofuel derived from edible plants, such as sugarcane Competes with edible crops, resulting in the high price of eatable items as well as feedstocks [63]
Second generation—Biofuel derived from non-edible parts of the plants, includes agricultural waste and switch grasses Demands excessive use of land, water, chemical fertilizers, and pesticides; non-fuel parts discarded, causing a disposal issue [64]
Advanced Energy Sources Third generation—Biofuel derived with the help of traditional microorganisms (algae, yeast, and bacteria) Does not compete with food crops; no demands of land, fertilizers, and pesticides; minimum use of land and water bodies [65]
Fourth generation—Biofuel derived with the help of genetically modified microorganisms with targeted efficiency An extensive increase in biofuel production due to the modification of targeted genes of the microbial cells [66]
Cost and benefit analysis indicates that first and second generation feedstocks demand significant agricultural land and other costlier resources that seriously affect food production in the agriculture sector. Furthermore, careful calculation and research in several reports have also pointed out that biodiesel production from third- and fourth-generation feedstocks has higher production costs than petroleum-derived diesel. Hence, only second-generation biodiesel production at the commercial level is currently feasible regarding cost and feedstock sustainability [67][68][69]. However, more than 95% of biodiesel production worldwide is from first-generation feedstocks, which are highly convenient as well as viable in those regions where resources for agriculture, such as land and water are available in surplus amounts [70][71][72]. However, alternative strategies such as transesterification (in Nostoc punctriforme), fermentation (in Synechococcus strain, Gloeocapsa alpicola, Anabaena sp.) and co-digestion with manure (in Lyngbya sp.) are also commercially viable for biofuel production [1][65][66][73][74][75] (Table 2).
Table 2. Biodesigned cyanobacterial strains for biofuel production.
Cyanobacteria Species/Strain Product(s) Biosynthetic Pathway/
Mechanism
Spirulina platensis, Anacystis nidulans Alkanes (C15–C17) Photosynthesis
Synechocystis sp. Butanol Fermentation
Nostoc punctriforme Biodisel Transesterification
Synechococcus strain Bioethanol Fermentation
Gloeocapsa alpicola, Anabaena sp. Biohydrogen Fermentation
Lyngbya sp. Biogas Co-digestion with manure
On the other hand, biodiesel’s preparation from edible oil crops will share the limited available cropland, resulting in a shortage of food supply. However, diesel from non-edible oil crops will not negatively impact food production and supply but will adversely affect the land and water resources. Researchers have already calculated the high cost of biodiesel synthesis from non-edible oils compared to petro-diesel. The natural bioavailability of algae, the most promising feedstock, has the potential to fulfill the demand for renewable energy–based fuel without any aid. Third-generation feedstocks may be the most widespread, as they include waste remnants of cooking oil, animal fats, plant fats, and effluent palm oil factories, which can be used for biodiesel production. Food processing industries do utilize a large amount of vegetable oil. In fact, biodiesel derived from oil-based waste is cheaper and more eco-friendly because no land or water resources are used and there is zero interference in the food chain supply.

3. Role of Cyanobacteria in High-Valued Biofuel

Cyanobacteria produce a wide range of metabolic products that are efficient substrates for biofuel production [74][75][76][77]. Stored macromolecules in cyanobacterial biomass are carbohydrates, lipid/fatty acids and proteins having the respective caloric value depending on the end product. Carbohydrate has calorific value of 26.72 and 32.5 kJ/g when its end products are bioethanol and biobutanol respectively. On the other hand, lipid/fatty acids have calorific value of 37.27 kJ/g producing the biodiesel. Besides cultivation, other steps, such as harvesting, extraction, and fuel production, are also money demanding steps. However, cyanobacteria are cultivated as biofilm, which curtails the costlier biomass harvesting step, reducing the total capital input (Table 3).
Table 3. Chemical composition of biofuel derived from cyanobacteria [77].
Nature of Stored Macromolecules Calorific Value (kJ/g) Derived Fuel
Carbohydrates 26.72 Bioethanol
32.5 Biobutanol
Lipid/fatty acids 37.27 Biodiesel
Carbohydrates and proteins 150.00 Biohydrogen
43.00 Biogas
Furthermore, chemical flocculation [76][77] methods using inorganic (i.e., lime and aluminum sulfate) and organic compounds (i.e., chitosan and polyelectrolyte) increase the harvesting of cyanobacterial biomass significantly. Many cyanobacterial species such as Spirulina platensis, Anabaena, and Microcystis have gas vesicles inside their cytoplasm that impart to them a type of natural flotation property, facilitating cheaper harvesting of cyanobacterial biomass. The supplementary addition of NaCl to Spirulina biofilm results in the flotation of up to 80% of the total biomass within a few hours, offering a cheaper and more effective harvesting approach. After harvesting, the drying and dewatering of the biomass is the next step for biofuel production [78][79]. The following Figure 1 gives a clear-cut illustration of the steps from microalgal growth optimization to biofuel production, including strain development and the possibility of incorporating the cultivation of algae with an existing setup of wastewater treatment. Inorganic carbon uptake is an essential process for the highest rate of the production of fuels with biological origin from cyanobacteria [80].
Sustainability 15 10439 g001
Figure 1. Modified cultivation and harvesting technique of cyanobacterial biomass.

3.1. Biodiesel from Cyanobacteria

Biochemically, biodiesel comprises long-chain fatty acids of mono-alkyl ester, the best alternative to petroleum-derived diesel. The initial processes of the generation of conventional jet fuel and biodiesel are synthesized by transesterification, where lipids or bio-oils, i.e., triglycerides, are serially converted into esters via diglycerides and monoglycerides. Significant components of biodiesel, such as glycerol and fatty acid methyl esters (FAMEs), can be obtained from the methanol and fatty acids (FAs) in the presence of a strong acid or base catalyst [81][82][83]. This synthesis pathway results in the end product of lipids, which is significantly beneficial, as lipids store more energy in comparison to carbohydrates [84]. Moreover, esters of biodiesel can be easily formed from cellular lipids. Biofuel can be directly produced from the extracts of lipids by blending the cyanobacterial biomass with alcohol and a heterogeneous catalyst under high temperature. In this process, cells of cyanobacteria undergo reactions with methanol in the presence of strong acid catalyst such as sulfuric acid inside a microwave reactor resulting in the transesterification of FAs followed by chloroform: methanol phase separation [85][86]. When total lipids undergo direct transesterification, the FA profile is enriched in total, as observed in the cyanobacterial biomass of Synechocystis sp. and Synechococcus elongates. The above procedure of chemical preparation results in high economic and logistical benefits as compared to plant-derived biodiesel derived from terrestrial crops such as soybean and corn. It is also possible to produce biodiesel from algae farmed in ponds on a very large scale when compared with the yield of biofuel from fuel crops, e.g., soya or rapeseed [87][88][89][90][91]. In fact, the above way of deriving cyanobacterial biofuel is advantageous for the environment, as it presents low sulfur emissions, zero production of aromatic hydrocarbons, the release of oxygen, and good combustion capacity.
Recently, some studies have focused on the development of innovative techniques for the extraction of crucial components of biofuel, such as methyl ester from algae, which offers compatibility with conventional diesel engines. However, the variation in the degree of competitiveness of cyanobacterial triacylglycerides in relation to other lipids derived from algal biomass hinders the commercial establishment of biofuel in the transportation industry [85]. Different species of cyanobacteria contain varying concentrations of fats, reaching a peak of 60% of their overall weight.
Scientific interest in algal oil is not a new trend, but its application for biofuel production is a recent trend in research communities. Algal oil, especially that from macroalgae, such as seaweed, is principally used in cosmetic industries. The various chemical compositions of different algal species indicate an average presence of 40% fat of their overall mass in most of the cyanobacterial species [86]; lipid and FA concentrations of microalgae may change depending on the culture conditions. In algal oil, saturated and monounsaturated FAs, such as oleic (18:1), palmitic (16:0), stearic (18:0), linoleic (18:2), and caproic acids (C6:0), are reported to be found. Algae accumulate 30–80% of lipids, usually in the form of 90–95% triacylglycerides. Wastewater cultivation is considered cost-effective in increasing the production of biomass and altering the concentration of FAs and lipid composition. Among the algal strains, Chlorella produces FAs in the range of C16–C18, which are considered suitable for the production of biodiesel, having similar properties as fossil-based biodiesel [87][88][89].

3.2. Bioethanol from Cyanobacteria

Bioethanol is synthesized by the fermentation of carbohydrates extracted from the algae or plants such as corn, sugarcane, wheat, and lignocellulosic biomass. First-generation bioethanol production, which is the traditional way of alcoholic fermentation, utilizes food crops as feedstocks (e.g., wheat, corn, potatoes, beets, sugarcane). These crops are excellent feedstocks for fermentation as they have very high indexes of starch and sugar and are easily available in the agro-sector. However, as the human population increases, putting more and more burdens on the limited agricultural land, serious concern arises over the fuel generation from food crops. Therefore, different sources, especially from the biomass of non-edible crops such as lignocellulosic materials and algae, are being examined as feedstocks for sustainable bioethanol production at the commercial level. Therefore, bioethanol generation can be carried out by utilizing feedstocks from non-food crops. However, specific cyanobacterial strains producing complex carbohydrates also result in the production of synthetic gas and bioethanol, similar to non-edible crops.
Carbohydrates extracted from the biomass of cyanobacteria can be changed to bioethanol by following the processes of hydrolysis and fermentation, which have various steps. Cytosolic sugars, often without oxygen, are channelized in glycolysis to produce free energy through fermentation, generating ethanol and CO2. As a source of fuel, bioethanol is commonly considered, as it has wide applications in existing diesel engines without any significant modification. The hydrolysis of Synechococcus sp. biomass through an enzymatic process followed by fermentation with the help of yeast tremendously increases the yield of ethanol quantity. Since glycogen, the storage form of carbohydrates, requires less storage volume inside the cell, it is preferred over the other forms of carbohydrates as a feedstock for bioethanol production [90][91][92]. Approximately 86% of ethanol production can be obtained through the fermentation of Synechococcus sp. with the help of yeast Saccharomyces cerevisiae. Cyanobacteria can be subjected to chemical hydrolysis for the lipid extraction process, thus fulfilling the dual purpose of recovering fermented carbohydrates and fats from the biomass. The evaluation of fat content in microalgae Tribonema sp. before and after hydrolysis indicates a 25% increase in its production. In addition to this, dark-fermentation and photo-fermentation processes are also employed to generate ethanol, the efficiency of which relies on the metabolic requirements of the cyanobacteria [93][94]. Algal species such as Chlamydomonas, Spirulina, Euglena, Chlorella, Scenedesmus, and Dunaliella have been vastly investigated for the production of bioethanol. The sugar content of algae can also be employed to generate biobutanol, biomethane, biogas, and syngas [95][96][97][98][99][100]. However, some countries manage to produce bioethanol from the feedstock of food crops. Brazil is totally dependent on sugarcane for bioethanol production to fulfill its requirement to a large extent.
Most of the conducted studies show that bioethanol production efficiency improves when cyanobacteria contain a lesser amount of lignocellulosic material as compared to higher plants. It shows that a lack of lignocellulosic biochemical can enhance the fermentation process [101].

3.3. Biobutanol from Cyanobacteria

In past few decades, direct production of short chain fuels like butanol has increased and providing an efficient way to the large scale production of technologies for alternative energy. Butanol is a 4-carbon alcohol (C4H9OH), a special bulk chemical and excellent blend in fossil fuel. Cyanobacteria such as Oscillatoria obscura and Lyngba limnetica are being used for biobutanol production at the commercial level by using Clostridium beijerinckii ATCC 35,702 as the fermenting microorganism in the presence of glucose. The productivity of biobutanol from these cyanobacteria is found to be 1.565 g/L, obtained with the supplement of glucose in the batch mode condition [89]. Furthermore, the genetically modified cyanobacteria Synechocystis PCC6803 sp. have been observed to emit fewer GHGs (3.1 kg CO2 eq/kg biobutanol) into the environment. However, genetically engineered microorganisms are being tested for sustainable biobutanol production [102][103][104][105][106].

3.4. Biohydrogen from Cyanobacteria

Biohydrogen is another fuel source that is renewable and yields H2O as the primary waste from its combustion reaction. It has been revealed that Anabaena spp. produces a high quantity of H2 [107][108]. It is known as a clean biofuel, having the highest energy density and eco-friendly production. Cyanobacteria perform biophotolysis of water molecules in the presence of sunlight for biohydrogen production. However, genetically modified cyanobacterial species have a greater potential for biohydrogen production, especially in fuel cells, hydrocarbon liquefaction, and excellent-quality heavy oils [107][108][109][110]. Biohydrogen can also be generated by cyanobacteria grown in an N2-deficient environment through the reversible activity of hydrogenase enzymes. Hydrogen production from cyanobacteria requires the presence of nitrogen-fixing strains having bidirectional hydrogenase, hydrogenase, and nitrogenase enzymes. It may be noted that non-heterocystous cyanobacteria are less efficient at H2 gas generation than heterocysts.
Among the different biohydrogen production techniques, biophotolysis, a dark-fermentation method, directly or indirectly uses carbohydrates from cyanobacteria to synthesize biohydrogen [111]. It is noted that a maximum of twelve moles of molecular hydrogen is produced per mole of glucose, as shown in the following formula:
C6H12O6 + 6H2O → 6CO2 + 12H2
The microbial bioelectric fuel cell is the greenest and the most sustainable biohydrogen production method for eco-friendly green fuel production. In most microbial fuel cells (MFCs), anodes are constructed from a cyanobacterial strain for hydrogen production, and cathodes are constructed from microalgae for oxygen production. They exploit microorganisms for the production of biohydrogen and are necessary for the function of fuel cells. The most significant benefits of these biological processes for energy production are the bioremediation activities.

3.5. Biogas Production from Cyanobacteria Waste

Cyanobacteria also produce gaseous fuels such as syngas for biofuel purposes. The residual biomass of cyanobacteria is converted into biogas through several conversion pathways. These processes pass through hydrolysis and fermentation, converting soluble glucose constituents into alcohols and other intermediate biogas products. The biogas produced in this way contains a mixture of methane, carbon dioxide, and hydrogen [107]. Some trace elements in cyanobacteria, along with nutrients such as proteins, lipids, and carbohydrates, can stimulate the process of methanogenesis for biogas formation. Biogas has been shown to depend on the quality of biomass and the pretreatment process. Among cyanobacterial strains evaluated for biogas production, Spirulina species has a conversion efficiency of up to 59% at 35 °C. Wastepaper sludge pretreated with cyanobacterial Phormidium valderianum strain enhances biodegradation and improves methane production efficiency. Balancing the carbon and nitrogen ratio increases cellulase activity and produces significant amounts of methane. Cyanobacteria also degrade harmful bioconstituents, such as cyanides, and convert them to methane, a unique biological capability found in certain cyanobacteria, such as Anabaena sp. Arthrospira platensisis is also reported to remove carbon dioxide from sewage sludge [112][113]. These supplementary techniques are expected to decrease the production cost of biofuel, which is viable for the bioenergy process.

This entry is adapted from the peer-reviewed paper 10.3390/su151310439

References

  1. Vij, R.K.; Subramanian, D.; Pandian, S.; Hari, S. A review of different technologies to produce fuel from micro-algal feedstock. Environ. Technol. Innov. 2021, 22, 101389.
  2. Feng, S.; Kang, K.; Salaudeen, S.; Ahmadi, A.; He, Q.S.; Hu, Y. Recent Advances in Algae-Derived Biofuels and Bioactive Compounds. Ind. Eng. Chem. Res. 2022, 61, 1232–1249.
  3. Apollon, W.; Rusyn, I.; Gonzalez-Gamboa, N.; Kuleshova, T.; Vidales-Contreras, A.; Kamraj, S.K. Improvement of zero waste sustainable recovery using microbial energy generation systems: A comprehensive review. Sci. Total Environ. 2022, 817, 153055.
  4. Zhang, H.; Zhang, X.; Ding, L.; Ma, J.; Kong, Y. Characteristics of Cyanobacterial Biomass Gasification in Sub- and Supercritical Water. Energy Fuels 2019, 33, 3239–3247.
  5. Zhang, Y.; Cha, S.; Feng, W.; Xu, G. Energy Sources for Road Transport in the Future. ACS Energy Lett. 2017, 2, 1334–1336.
  6. Alper, K.; Tekin, K.; Karago, S.; Ragauskas, A.J. Sustainable energy and fuels from biomass: A review focusing on hydrothermal bio-mass processing. Sustain. Energy Fuels 2020, 4, 4390–4414.
  7. Smith, C.; Hill, A.K.; Torrente-Murciano, L. Current and future role of Haber–Bosch ammonia in a carbon-free energy land-scape. Energy Environ. Sci. 2020, 13, 331–344.
  8. Beer, J.D. Potential for Industrial Energy-Efficiency Improvement in the Long Term; Kluwer Academic: Dordrecht, The Netherlands; Boston, MA, USA, 2000.
  9. Trout, K.; Muttitt, G.; Lafleur, D.; de Graaf, T.V.; Mendelevitch, R.; Mei, L. Existing fossil fuel extraction would warm the world beyond 1.5 °C. Environ. Res. Lett. 2022, 17, 064010.
  10. Matthews, H.D. A growing commitment to future CO2 emissions. Environ. Res. Lett. 2014, 9, 11.
  11. Jain, V. Fossil Fuels, GHG Emissions and Clean Energy Development: Asian Giants in a Comparative Perspective. Millenn. Asia 2019, 10, 1–24.
  12. Albuquerque, F.D.; Maraqa, M.A.; Chowdhury, R.; Mauga, T.; Alzard, M. Greenhouse gas emissions associated with road transport projects: Current status, benchmarking, and assessment tools. Transp. Res. Procedia 2020, 48, 2018–2030.
  13. Huijbregts, M.A.J.; Rombouts, L.J.A.; Hellweg, S.; Frischknecht, R.; Hendriks, A.J.; Van De Meent, D.; Ragas, A.M.J.; Reijnders, L.; Struijs, J. Is Cumulative Fossil Energy Demand a Useful Indicator for the Environmental Performance of Products? Environ. Sci. Technol. 2006, 40, 641–648.
  14. Thomas, V.M.; Graedel, T.E. Research Issues in Sustainable Consumption: Toward an Analytical Framework for Materials and the Environment. Environ. Sci. Technol. 2003, 37, 5383–5388.
  15. Moriarty, P.; Honnery, D. The Transition to Renewable Energy: Make Haste Slowly. Environ. Sci. Technol. 2011, 45, 2527–2528.
  16. Sharma, S.; Kundu, A.; Basu, S.; Shetti, N.P.; Aminabhavi, T.M. Sustainable environmental management and related biofuel technologies. J. Environ. Manag. 2020, 273, 111096.
  17. Ullah, K.; Sharma, V.K.; Ahmad, M.; Lv, P.; Krahl, J.; Wang, Z.; Sofia. The insight views of advanced technologies and its application in bio-origin fuel synthesis from lignocellulose biomasses waste, a review. Renew Sustain. Energy Rev. 2018, 82, 3992–4008.
  18. De, S.; Saha, B.; Luque, R. Hydrodeoxygenation processes: Advances on catalytic transformations of biomass-derived plat-form chemicals into hydrocarbon fuels. Bioresour. Technol. 2015, 178, 108–118.
  19. Stephen, J.L.; Periyasamy, B. Innovative developments in biofuels production from organic waste materials: A review. Fuel 2018, 214, 623–633.
  20. Li, Y.; Kesharwani, R.; Sun, Z.; Qin, R.; Dagli, C.; Zhang, M.; Wang, D. Economic viability and environmental impact investi-gation for the biofuel supply chain using co-fermentation technology. Appl. Energy 2020, 259, 114235.
  21. Ibrahim, M.F.; Ramli, N.; Bahrin, E.K.; Abd-Aziz, S. Cellulosic biobutanol by Clostridia: Challenges and improvements. Renew. Sustain. Energy Rev. 2017, 79, 1241–1254.
  22. In-Na, P.; Lee, J.; Caldwell, G. Living textile biocomposites deliver enhanced carbon dioxide capture. J. Ind. Text. 2021, 51, 5683S–5707S.
  23. Moreira, D.; Pires, J.C. Atmospheric CO2 capture by algae: Negative carbon dioxide emission path. Bioresour. Technol. 2016, 215, 371–379.
  24. Do Nascimento, M.; Rizza, L.S.; Di Palma, A.A.; de los Angeles Dublan, M.; Salerno, G.; Rubio, L.M.; Curatti, L. Cyanobacterial biological nitrogen fixation as a sustainable nitrogen fertilizer for the production of microalgal oil. Algal Res. 2015, 12, 142–148.
  25. Singh, J.; Dhar, D.W. Overview of Carbon Capture Technology: Microalgal Biorefinery Concept and State-of-the-Art. Front. Mar. Sci. 2019, 6, 29.
  26. Yashveer, S. Photosynthetic activity and lipid and hydracarbon production by alginate immobilized cells of botryococcus in relation to growth phase. J. Microbiol. Biotechnol. 2003, 13, 687–691.
  27. Wijffels, R.H.; Kruse, O.; Hellingwerf, K.J. Potential of industrial biotechnology with cyanobacteria and eukaryotic micro-algae. Curr. Opin. Biotechnol. 2013, 24, 405–413.
  28. Converti, A.; Casazza, A.A.; Ortiz, E.Y.; Perego, P.; Del Borghi, M. Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chem. Eng. Process. Process Intensif. 2009, 48, 1146–1151.
  29. Illman, A.; Scragg, A.; Shales, S. Increase in Chlorella strains calorific values when grown in low nitrogen medium. Enzym. Microb. Technol. 2000, 27, 631–635.
  30. Farrokh, P.; Sheikhpour, M.; Kasaeian, A.; Asadi, H.; Bavandi, R. Cyanobacteria as an eco-friendly resource for biofuel pro-duction: A critical review. Biotechnol. Prog. 2019, 35, e2835.
  31. Rajneesh; Singh, S.P.; Pathak, J.; Sinha, R.P. Cyanobacterial factories for the production of green energy and value-added products: An integrated approach for economic viability. Renew. Sustain. Energy Rev. 2017, 69, 578–595.
  32. Perera, F. Pollution from fossil-fuel combustion is the leading environmental threat to global pediatric health and equity: Solutions exist. Int. J. Environ. Res. Public Health 2018, 15, 16.
  33. Sarsekeyeva, F.; Zayadan, B.K.; Usserbaeva, A.; Bedbenov, V.S.; Sinetova, M.A.; Los, D.A. Cyanofuels: Biofuels from cya-nobacteria, reality and perspectives. Photosynth. Res. 2015, 125, 329–340.
  34. Demirbas, A. Use of algae as biofuel sources. Energy Convers. Manag. 2010, 51, 2738–2749.
  35. Sarma, M.K.; Kaushik, S.; Goswami, P. Cyanobacteria: A metabolic power house for harvesting solar energy to produce bio-electricity and biofuels. Biomass-Bioenergy 2016, 90, 187–201.
  36. Sánchez-Baracaldo, P.; Bianchini, G.; Wilson, J.D.; Knoll, A.H. Cyanobacteria and biogeochemical cycles through earth history. Trends Microbiol. 2022, 30, 143–157.
  37. Simons, M.J. The Evolution of the Cyanobacterial Posttranslational Clock from a Primitive “Phoscillator”. J. Biol. Rhythm. 2009, 24, 175–182.
  38. Shoener, D.B.; Schramm, S.M.; Beline, F.; Bernard, O.; Martínez, C.; Plosz, B.G.; Snowling, S.; Valverde-Perez, B.; Wagner, D.; Guest, J.S. Microalgae and cyanobacteria modeling in water resource recovery facilities: A critical review. Water Res. X 2019, 2, 100024.
  39. Baroukh, C.; Munoz-Tamayo, R.; Steyer, J.-P.; Bernard, O. A state of the art of ~ metabolic networks of unicellular mi-croalgae and cyanobacteria for biofuel production. Metab. Eng. 2015, 30, 49–60.
  40. Singh, Y.; Gulati, A.; Singh, D.P.; Khattar, J.I.S. Cyanobacterial community structure in hot water springs of Indian North Western Himalayas: A morphological, molecular and ecological approach. Algal Res. 2018, 29, 179–192.
  41. Gualtieri, P. Morphology of photoreceptor systems in microalgae. Micron 2000, 32, 411–426.
  42. Shahid, A.; Usman, M.; Atta, Z.; Musharraf, S.G.; Malik, S.; Elkamel, A.; Shahid, M.; Alkhattabi, N.A.; Gull, M.; Mehmood, M.A. Impact of wastewater cultivation on pollutant removal, biomass production, metabolite biosynthesis, and carbon dioxide fixation of newly isolated cyanobacteria in a multiproduct biorefinery paradigm. Bioresour. Technol. 2021, 333, 125194.
  43. Sood, A.; Renuka, N.; Prasanna, R.; Ahluwalia, A.S. Cyanobacteria as potential options for wastewater treatment. In Phytoremediation; Ansari, A.A., Ed.; Springer: Cham, Switzerland, 2015; Volume 2.
  44. Badr, O.A.M.; EL-Shawaf, I.I.S.; El-Garhy, H.A.S.; Moustafa, M.M.A.; Ahmed-Farid, O.A. Antioxidant activity and phyco remediation ability of four cyanobacterial isolates obtained from a stressed aquatic system. Mol. Phylogenetics Evol. 2019, 134, 300–310.
  45. Kondi, V.; Sabbani, V.; Alluri, R.; Karumuri, T.S.K.; Chawla, P.; Dasrapur, S.; Tiwari, O.N. Chapter 4—Cyanobacteria as potential bio resources for multifaceted sustainable utilization. New Future Dev. Microb. Biotechnol. Bioeng. 2022, 2022, 73–87.
  46. Bighiu, M.A.; Goedkoop, W. Interactions with freshwater biofilms cause rapid removal of common herbicides through degradation—Evidence from microcosm studies. Environ. Sci. Process. Impacts 2020, 23, 66–72.
  47. Pulz, O.; Gross, W. Valuable products from biotechnology of microalgae. Appl. Microbiol. Biotechnol. 2004, 65, 635–648.
  48. Colla, L.M.; Reinehr, C.O.; Reichert, C.; Costa, J.A.V. Production of biomass and nutraceutical compounds by Spirulina platensis under different temperature and nitrogen regimes. Bioresour. Technol. 2007, 98, 1489–1493.
  49. Knoot, C.J.; Ungerer, J.; Wangikar, P.P.; Pakrasi, H.B. Cyanobacteria: Promising biocatalysts for sustainable chemical production. J. Biol. Chem. 2018, 293, 5044–5052.
  50. Subashchandrabose, S.R.; Ramakrishnan, B.; Megharaj, M.; Venkateswarlu, K.; Naidu, R. Consortia of cyanobacteria/microalgae and bacteria: Biotechnological potential. Biotechnol. Adv. 2011, 29, 896–907.
  51. Singh, J.; Gu, S. Commercialization potential of microalgae for biofuels production. Renew. Sustain. Energy Rev. 2010, 14, 2596–2610.
  52. Scott, S.A.; Davey, M.P.; Dennis, J.S.; Horst, I.; Howe, C.J.; Lea-Smith, D.J.; Smith, A.G. Biodiesel from algae: Challenges and prospects. Curr. Opin. Biotechnol. 2010, 21, 277–286.
  53. Mendoza, A.; Vicente, G.; Bautista, L.F.; Morales, V. Opportunities for biomass through the isolation of its components and biodiesel production. Green Process. Synth. 2015, 4, 97–102.
  54. Kim, B.-H.; Kang, Z.; Ramanan, R.; Choi, J.-E.; Cho, D.-H.; Oh, H.-M.; Kim, H.-S. Nutrient Removal and Biofuel Production in High Rate Algal Pond Using Real Municipal Wastewater. J. Microbiol. Biotechnol. 2014, 24, 1123–1132.
  55. Abdel-Raouf, N.; Al-Homaidan, A.A.; Ibraheem, I.B.M. Microalgae and wastewater treatment. Saudi J. Biol. Sci. 2012, 19, 257–275.
  56. El-Sheekh, M.M.; El-Shouny, W.A.; Osman, M.E.; El-Gammal, E.W.; El-Gammal, E. Treatment of sewage and industrial wastewater effluents by the cyanobacteria Nostoc muscorum and Anabaena subcylinderica. J. Water Chem. Technol. 2014, 36, 190–197.
  57. Tsolcha, O.N.; Tekerlekopoulou, A.G.; Akratos, C.S.; Aggelis, G.; Genitsaris, S.; Moustaka-Gouni, M.; Vayenas, D.V. Agroindustrial Wastewater Treatment with Simultaneous Biodiesel Production in Attached Growth Systems Using a Mixed Microbial Culture. Water 2018, 10, 1693.
  58. Arbib, Z.; Ruiz, J.; Álvarez-Díaz, P.; Garrido-Pérez, C.; Perales, J. Capability of different microalgae species for phytoremedi-ation processes: Wastewater tertiary treatment, CO2 bio-fixation and low cost biofuels production. Water Res. 2014, 49, 465–474.
  59. Anjaneyulu, B.; Kaki, S.S.; Kanjilal, S.; Reddy, J.R.C.; Ravinder, T.; Prasad, R.B.N.; Rao, B.V.S.K. Physico-chemical characterization and biodiesel preparation from ailanthus excelsa seed oil, Energy Sources. Part A Recovery Util. Environ. Eff. 2017, 8, 811–817.
  60. Tabatabai, B.; Chen, H.; Lu, J.; Giwa-Otusajo, J.; McKenna, A.M.; Shrivastava, A.K.; Sitther, V. Fremyella diplosiphon as a Biodiesel Agent: Identification of Fatty Acid Methyl Esters via Microwave-Assisted Direct In Situ Transesterification. BioEnergy Res. 2018, 11, 528–537.
  61. de Oliveira, D.T.; Vasconcelos, C.T.; Feitosa, A.M.T.; Aboim, J.B.; de Oliveira, A.D.N.; Xavier, L.P.; Santos, A.S.; Gonçalves, E.C.; da Rocha Filho, G.N.; do Nascimento, L.A.S. Lipid profile analysis of three new amazonian cyanobacteria as potential sources of biodiesel. Fuel 2018, 234, 785–788.
  62. Johnson, T.J.; Jahandideh, A.; Johnson, M.D.; Fields, K.H.; Richardson, J.W.; Muthukumarappan, K.; Cao, Y.; Gu, Z.; Halfmann, C.; Zhou, R.; et al. Producing next-generation biofuels from filamentous cyanobacteria: An economic feasibility analysis. Algal Res. 2016, 20, 218–228.
  63. Oluwoye, I.; Altarawneh, M.; Gore, J.; Dlugogorski, B.Z. Products of incomplete combustion from biomass reburning. Fuel 2020, 274, 117805.
  64. Rulli, M.C.; Bellomi, D.; Cazzoli, A.; De Carolis, G.; D’odorico, P. The water-land-food nexus of first-generation biofuels. Sci. Rep. 2016, 6, 22521.
  65. Bhuiy, M.M.K.; Rasul, M.G.; Khan, M.M.K.; Ashwath, N.; Azad, A.K.; Hazrat, M.A. Second generation biodiesel: Potential alternative toedible oil-derived biodiesel. Energy Procedia 2014, 61, 1969–1972.
  66. Leong, W.H.; Lim, J.W.; Lam, M.K.; Uemura, Y.; Ho, Y.C. Third generation biofuels: A nutritional per-spective in enhancing microbial lipid production. Renew Sustain. Energy Rev. 2008, 91, 950–961.
  67. Abdullah, B.; Muhammad, S.A.F.S.; Shokravi, Z.; Ismail, S.; Kassim, K.A.; Mahmood, A.N.; Aziz, M.A. Fourth generation biofuel: A review on risks and mitigation strategies. Renew. Sustain. Energy Rev. 2019, 107, 37–50.
  68. Maliha, A.; Abu-Hijleh, B. A review on the current status and post-pandemic prospects of third-generation biofuels. Energy Syst. 2022.
  69. Mahyari, Z.F.; Khorasanizadeh, Z.; Khanali, M.; Mahyari, K.F. Biodiesel production from slaughter wastes of broiler chicken: A potential survey in Iran. SN Appl. Sci. 2021, 3, 57.
  70. AKanakdande, P.; Khobragade, C.N. Biodiesel synthesis from non edible oil using agro waste and evaluation of its physicochemical properties. Int. J. Eviron. Sci. Technol. 2020, 17, 3785–3800.
  71. Furtado, A.; Lupoi, J.S.; Hoang, N.V.; Healey, A.; Singh, S.; Simmons, B.A.; Henry, R.J. Modifying plants for biofuel and biomaterial production. Plant Biotechnol. J. 2014, 12, 1246–1258.
  72. Rezki, B.; Essamlali, Y.; Aadil, M.; Semlal, N.; Zahouily, M. Biodiesel production from rapeseed oil and low free fatty acid waste cooking oil using a cesium modified natural phosphate catalyst. RSC Adv. 2020, 10, 41065–41077.
  73. Hachicha, R.; Elleuch, F.; Ben Hlima, H.; Dubessay, P.; de Baynast, H.; Delattre, C.; Pierre, G.; Hachicha, R.; Abdelkafi, S.; Michaud, P.; et al. Biomolecules from microalgae and cyanobacteria: Applications and market survey. Appl. Sci. 2022, 12, 1924.
  74. Velmurugan, R.; Incharoensakdi, A. Metabolic transformation of cyanobacteria for biofuel production. Chemosphere 2022, 299, 134342.
  75. Peng, L.; Lei, L.; Xiao, L.; Han, B. Cyanobacterial removal by a red soil-based flocculant and its effect on zooplankton: An experiment with deep enclosures in a tropical reservoir in China. Environ. Sci. Pollut. Res. 2018, 26, 30663–30674.
  76. Vandamme, D.; Foubert, I.; Muylaert, K. Flocculation as a low-cost method for harvesting microalgae for bulk biomass production. Trends Biotechnol. 2013, 31, 233–239.
  77. Quintana, N.; Van der Kooy, F.; Van de Rhee, M.D.; Voshol, G.P.; Verpoorte, R. Renewable energy from Cyanobacteria: Energy production optimization by metabolic pathway engineering. Appl. Microbiol. Biotechnol. 2011, 91, 471–490.
  78. Sandrini, G.; Matthijs, H.C.P.; Verspagen, J.M.H.; Muyzer, G.; Huisman, J. Genetic diversity of inorganic carbon uptake systems causes variation in CO2 response of the cyanobacterium Microcystis. ISME J. 2013, 8, 589–600.
  79. Zulqarnain; Yusoff, M.H.M.; Ayoub, M.; Jusoh, N.; Abdullah, A.Z. The Challenges of a Biodiesel Implementation Program in Malaysia. Processes 2020, 8, 1244.
  80. Krishania, N.; Rajak, U.; Verma, T.N.; Birru, K.A.; Pugazhendhi, A. Effect of microalgae, tyre pyrolysis oil and Jatropha bio-diesel enriched with diesel fuel on performance and emission characteristics of CI engine. Fuel 2020, 278, 118252.
  81. Agarwal, P.; Soni, R.; Kaur, P.; Madan, A.; Mishra, R.; Pandey, J.; Singh, S.; Singh, G. Cyanobacteria as a Promising Alternative for Sustainable Environment: Synthesis of Biofuel and Biodegradable Plastics. Front. Microbiol. 2022, 13, 939347.
  82. Anahas, A.M.P.; Muralitharan, G. Characterization of heterocystous cyanobacterial strains for biodiesel production based on fatty acid content analysis and hydrocarbon production. Energy Convers. Manag. 2018, 157, 423–437.
  83. Sivagurulingam, A.P.A.; Sivanandi, P.; Pandian, S. Isolation, mass cultivation, and biodiesel production potential of marine microalgae identified from Bay of Bengal. Environ. Sci. Pollut. Res. 2022, 29, 6646–6655.
  84. Anahas, A.M.P.; Muralitharan, G. Isolation and screening of heterocystous cyanobacterial strains for biodiesel production by evaluating the fuel properties from fatty acid methyl ester (FAME) profiles. Bioresour. Technol. 2015, 184, 9–17.
  85. Sharafi, H.; Fooladi, J.; Tabatabaei, M.; Heravi, M.M.; Memar, H.R. Lipid production capacity of a newly characterized cyanobacterial strain synechocystis sp. MH01: A comparative performance evaluation of cyanobacterial lipid-based biodiesel. Iran. J. Biotechnol. 2021, 19, e2313.
  86. Luque, R.; Lovett, J.C.; Datta, B.; Clancy, J.; Campelo, J.M.; Romero, A.A. Biodiesel as feasible petrol fuel replacement: A multidisciplinary overview. Energy Environ. Sci. 2010, 3, 1706–1721.
  87. Li, R.; Watanabe, M.M. Fatty acid profiles and their chemotaxonomy in planktonic species of Anabaena (Cyanobacteria) with straight trichomes. Phytochemistry 2001, 57, 727–731.
  88. Jawaharraj, K.; Karpagam, R.; Ashokkumar, B.; Kathiresan, S.; Varalakshmi, P. Green renewable energy production from Myxosarcina sp.: Media optimization and assessment of biodiesel fuel properties. RSC Adv. 2015, 5, 51149–51157.
  89. Aboim, J.B.; Oliveira, D.; Ferreira, J.E.; Siqueira, A.S.; Dall’Agnol, L.T.; Filho, G.N.R.; Gonçalves, E.C.; Nascimento, L.A. Determination of biodiesel properties based on a fatty acid profile of eight Amazon cyanobacterial strains grown in two different culture media. RSC Adv. 2016, 6, 109751–109758.
  90. Converti, A.; Oliveira, R.P.; Torres, B.R.; Lodi, A.; Zilli, M. Biogas production and valorization by means of a two-step bio-logical process. Bioresour. Technol. 2009, 100, 5771–5776.
  91. Sánchez, Á.; Maceiras, R.; Cancela, Á.; Pérez, A. Culture aspects of Isochrysis galbana for biodiesel production. Appl. Energy 2013, 101, 192–197.
  92. Jahromi, K.G.; Koochi, Z.H.; Kavoosi, G.; Shahsavar, A. Manipulation of fatty acid profle and nutritional quality of chlorella vulgaris by supplementing with citrus peel fatty acid. Sci. Rep. 2022, 12, 8151.
  93. Catalanotti, C.; Yang, W.; Posewitz, M.C.; Grossman, A.R. Fermentation metabolism and its evolution in algae. Front. Plant Sci. 2013, 4, 150.
  94. Eshaq, F.S.; Ali, M.N.; Mohd, M.K. Production of bioethanol from next generation feed-stock alga Spirogyra species. Int. J. Eng. Sci. Technol. 2011, 3, 1749–1755.
  95. Adams, J.M.; Gallagher, J.A.; Donnison, I.S. Fermentation study on Saccharina latissima for bioethanol production consid-ering variable pre-treatments. J. Appl. Phycol. 2009, 21, 569–574.
  96. Wang, M.; Luan, G.; Lu, X. Engineering ethanol production in a marine cyanobacterium Synechococcus sp. PCC7002 through simultaneously removing glycogen synthesis genes and introducing ethanolgenic cassettes. J. Biotechnol. 2020, 317, 1–4.
  97. Bautista, L.F.; Vicente, G.; Mendoza, A.; González, S.; Morales, V. Enzymatic production of biodiesel from Nannochloropsis gaditana microalgae using immobilized lipases in mesoporous materials. Energy Fuels 2015, 29, 4981–4989.
  98. Hwang, J.-H.; Church, J.; Lee, S.-J.; Park, J.; Lee, W.H. Use of Microalgae for Advanced Wastewater Treatment and Sustainable Bioenergy Generation. Environ. Eng. Sci. 2016, 33, 882–897.
  99. Halim, R.; Danquah, M.K.; Webley, P.A. Extraction of oil from microalgae for biodiesel production: A review. Biotechnol. Adv. 2012, 30, 709–732.
  100. Tesfaw, A.; Assefa, F. Current trends in bioethanol production by Saccharomyces cerevisiae: Substrate, inhibitor reduction, growth variables, coculture, and immobilization. Int. Sch. Res. Not. 2014, 8, 532852.
  101. Singh, A.; Bajar, S.; Bishnoi, N.R. Enzymatic hydrolysis of microwave alkali pretreated rice husk for ethanol production by Saccharomyces cerevisiae, Scheffersomyces stipitis and their co-culture. Fuel 2014, 116, 699–702.
  102. Brandt, B.A.; García-Aparicio, M.D.; Görgens, J.F.; Willem, H.; Zyl, V. Rational engineering of saccharomyces cerevisiae towards improved tolerance to multiple inhibitors in lignocellulose fermentations. Biotechnol. Biofuels 2021, 14, 173.
  103. Shen, Y.; Guo, J.-S.; Chen, Y.-P.; Zhang, H.-D.; Zheng, X.-X.; Zhang, X.-M.; Bai, F.-W. Application of low-cost algal nitrogen source feeding in fuel ethanol production using high gravity sweet potato medium. J. Biotechnol. 2012, 160, 229–235.
  104. Sivaramakrishnan, R.; Suresh, S.; Kanwal, S.; Ramadoss, G.; Ramprakash, B.; Incharoensakdi, A. Microalgal biorefinery concepts’ developments for biofuel and bioproducts: Current perspective and bottlenecks. Int. J. Mol. Sci. 2022, 23, 2623.
  105. Kushwaha, D.; Srivastava, N.; Prasad, D.; Mishra, P.K.; Upadhyay, S.N. Biobutanol production from hydrolysates of cyano-bacteria Lyngbya limnetica and Oscillatoria obscura. Fuel 2020, 271, 117583.
  106. Freire, D.V.; Ketzer, F.; Rösch, C. Advanced metabolic engineering approaches and renewable energy to improve environmental benefts of algal biofuels: LCA of large scale biobutanol production with cyanobacteria Synechocystis PCC6803. BioEnergy Res. 2022, 15, 1515–1530.
  107. Sharma, A.; Arya, S.K. Hydrogen from algal biomass: A review of production process. Biotechnol. Rep. 2017, 15, 63–69.
  108. Abo-Hashesh, M.; Wang, R.; Hallenbeck, P.C. Metabolic engineering in dark fermentative hydrogen production; theory and practice. Bioresour. Technol. 2011, 102, 8414–8422.
  109. Beer, L.L.; Boyd, E.S.; Peters, J.W.; Posewitz, M.C. Engineering algae for biohydrogen and biofuel production. Curr. Opin. Biotechnol. 2009, 20, 264–271.
  110. Srivastava, N.; Srivastava, M.; Singh, R.; Syed, A.; Pal, D.B.; Elgorban, A.M.; Kushwaha, D.; Mishra, P.; Gupta, V.K. Co-fermentation of residual algal biomass and glucose under the influence of Fe3O4 nanoparticles to enhance biohydrogen production under dark mode. Bioresour. Technol. 2021, 342, 126034.
  111. Zaidi, A.A.; RuiZhe, F.; Shi, Y.; Khan, S.Z.; Mushtaq, K. Nanoparticles augmentation on biogas yield from microalgal biomass anaerobic digestion. Int. J. Hydrogen Energy 2018, 43, 14202–14213.
  112. Bandyopadhyay, A.; Stöckel, J.; Min, H.; Sherman, L.A.; Pakrasi, H.B. High rates of photobiological H2 production by a cyanobacterium under aerobic conditions. Nat. Commun. 2010, 1, 139.
  113. Kamshybayeva, G.K.; Kossalbayev, B.D.; Sadvakasova, A.K.; Zayadan, B.K.; Bozieva, A.M.; Dunikov, D.; Alwasel, S.; Allakhverdiev, S.I. Strategies and economic feasibilities in cyanobacterial hydrogen production. Int. J. Hydrogen Energy 2022, 47, 29661–29684.
More
© Text is available under the terms and conditions of the Creative Commons Attribution (CC BY) license; additional terms may apply. By using this site, you agree to the Terms and Conditions and Privacy Policy.
This entry is offline, you can click here to edit this entry!
Video Production Service
Feedback