Pheromones Secreted by Nematodes: History
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Pheromones are chemical signals secreted by one individual that can affect the behaviors of other individuals within the same species. Ascaroside is an evolutionarily conserved family of nematode pheromones that play an integral role in the development, lifespan, propagation, and stress response of nematodes. Their general structure comprises the dideoxysugar ascarylose and fatty-acid-like side chains. Ascarosides can vary structurally and functionally according to the lengths of their side chains and how they are derivatized with different moieties.  Ascarosides (ASCRs) represent the majority of the pheromones secreted by nematodes. The molecular formula for an ascaroside, C33H68O4, was first proposed by Schulz and Becker in 1933. Different phenotypes of nematode species are produced by different ascarosides or combinations of ascarosides; even slight changes in the chemical structure tend to produce drastically different patterns of activity. As a rule, the patterns of the biosynthesis of ascarosides are linked to the phylogeny, lifestyle, and ecological niche of the organism. In addition, different concentrations of the same ascarosides can have different effects on nematodes. Other chemicals such as vanillic acid function as pheromones in some nematodes, but there have been comparatively few studies and discoveries in this area.

  • nematode pheromones
  • ascarosides
  • structures
  • functions

1. Development-Related Pheromones Secreted by Nematodes

The ability of nematodes to be so widely distributed in nature is closely related to their special developmental patterns. C. elegans lives freely in soil; it has a small, transparent body and serves as a model nematode species [1]. When the environment is suitable, an individual C. elegans starts to develop from a fertilized egg and progresses to adulthood through four stages of development. However, it stops feeding and developing if it encounters extreme conditions, such as a lack of food, elevated temperatures, or an increase in population density, and then the larva may enter a highly stress-resistant state called dauer diapause. This stage can last for several months. The nematodes eventually resume development and molt into the reproductive cycle under suitable conditions [2][3][4][5][6]. Much research shows that chemical pheromones can control dauer entry and exit [7][8].
The first dauer-inducing pheromone (daumone) was identified by means of the ethyl acetate extraction of a C. elegans liquid medium. The molecular structure of daumone was thereby determined to be (2)-(6R)-(3,5-dihydroxy-6-methyltetrahydropyran-2-yloxy) heptanoic acid, abbreviated to ascr#1 (also called daumone-1/ascaroside C7/asc-C7) (Table 1) [9]. Subsequently, ascr#2 (also called daumone-2/ascaroside C6) (Table 1) and ascr#3 (also called daumone-3/asc-∆C9/ascaroside C9) (Table 1) were also isolated and identified. Ascr#2 and ascr#3 induce dauer formation about 100 times more potently than ascr#1 does [10]. The pheromones that induce dauer formation may be single ascarosides or mixtures of different ascarosides, and these pheromones often act synergistically when mixed together. The dauer pheromone of C. elegans is mainly composed of ascr#2, ascr#3, and several other components, while in Caenorhabditis briggsae, the main component of the dauer pheromone is ascr#2 [11]. A derivative of ascr#2 has a β-glucosyl substituent linked to C2 of the ascarylose in ascr#2 and is named ascr#4 (also called daumone-4) (Table 1). The activity of ascr#4 is low [12]. Ascr#5 (also called daumone-5/ascaroside C3/asc-ωC3) (Table 1) is a potent dauer pheromone. The main function of ascr#5 is the activation of the axon regeneration pathway via SRG-36/SRG-37 GPCRs and EGL-30, indicating ascaroside signaling promotes axon regeneration by activating the GPCR-Gqα pathway [13]. In addition, the crh-1 gene plays an autonomous role in ascr#5, sensing ASI neurons in order to inhibit the dauer formation of C. elegans L2d [14]. Ascr#5 also produces synergistic effects with ascr#2 and ascr#3 [15]. In ASI neurons, ascaroside pheromones (compounds composed of ascr#2, ascr#3, and ascr#5) reversibly inhibit the expression of the str-3 chemical receptor gene, and when ascarosides are removed, its expression resumes. This process mainly occurs through the GPCR receptors SRBC-64/66 and SRG-36/37, which are required for str-3 repression [16]. Ascr#8 (Table 1) uniquely possesses a p-aminobenzoate group in its terminus; this group is a folate precursor that is derived from bacteria and is not synthesized by C. elegans [17].
P. pacificus is a model species that has been extensively studied in biology [18]. This nematode can enter the dauer stage or other stages if food is enough for growth [19][20]. Typically, the mouth of an adult that preys on other nematodes is more complex than that of a bacterivorous nematode. Pheromones can regulate the mouth dimorphism of P. pacificus [21]. Neelanjan et al. [22] analyzed fractions of the P. pacificus exo-metabolome and found that it has rich signaling molecules controlling adult phenotypic plasticity, including ascarosides ascr#1, 9, and 12. Pasc#9 (Table 1) was the most abundant derivative after pasc#1 and pasc#12. Pasc#9 comprises an N-succinyl 1-phenylethanolamide connected to ascarylose with a 4-hydroxypentanoic acid chain. Dasc#1 (Table 1) consists of two ascr#1 units; one ascr#1 unit is connected to carbon 4 of the other ascr#1 unit. A 3-ureido isobutyrate moiety is also present on carbon 4 of ubas#1 (Table 1), and ubas#1 also contains ascr#9 with the (ω)-oxygenated ascaroside oscr#9 connected at position 2 [22]. Recent studies revealed that the formation of ubas#1 and related metabolites specifically requires the putative carboxylesterase Ppa-uar-1 [23]. Additionally, dimeric ascarosides and ureido isobutyrate-substituted metabolites were first reported in P. pacificus. L-paratose forms the basis of part#9 (Table 1). Part#9 only differs from ascr#9 in terms of the stereochemistry of one hydroxyl group. Part#9 is also one of the components of npar#1. Npar#1 (Table 1) contains a derivative of the nucleoside adenosine. Although part#9, npar#1, ubas#1, and pasc#9 can induce dauer formation, npar#1 has a more intense effect than the others. Pasc#9, ascr#1, dasc#1, and npar#1 can induce eurystomatous mouth formation, a predatory morphology in the final larval and juvenile stages, in which P. pacificus-specific dasc#1 plays an important role [24][25].
Heterorhabditis bacteriophora is parasitic toward insects and has a developmental process similar to that of C. elegans. In the soil, the infective juveniles (IJs) survive as the only state of entomopathogenic nematodes. After IJs infect the host insects, they recover and lay eggs in their adults, which develop through four larval stages (J1–J4) to form the next generation [26][27][28]. In this process, H. bacteriophora secretes the ascaroside C11 ethanolamine (asc C11 EA) (Table 1), which prevents IJs from recovering to the J4 stage. Asc C11 EA comprises an ascarylose sugar, an ethanolamine fragment, and a carbon side chain containing ω-1 alcohol; the fatty-acid-derived portion of the side chain is 11 carbons long. Asc C11 EA and the dauer pheromone of C. elegans show structural similarity [29].
Figure 1 illustrates the schematic structure of the pheromones secreted by nematodes during their development. The figure presents a schematic diagram showing ascarylose sugars, variable-length fatty acids, and other moieties modifying them, which form different species of development-related ascaroside species.
Figure 1. Overview of the chemical structure of development-related pheromones secreted by nematodes. Nacq#1 does not have an ascaroside structure, so it is not shown here, and its detailed structure is shown in Table 1.

2. Sex Pheromones Secreted by Nematodes

Mate selection is universal in sexually reproducing organisms, and pheromones provide individuals with advantageous mating information that helps them to select high-quality mates. In the twentieth century, the first sex pheromone was named bombykol, which is released from female silk moths (Bombyx mori) [30]. Sex pheromones have since been researched in more depth; they are defined as chemical substances produced by individuals that cause innate and rigid sexual behavior [31]. These pheromones have both sex- and species-specific effects. Nematode mating behavior is also regulated by pheromones [32]. Generally, the nematode mating response can be induced when the pheromone concentration is much lower than the concentration required for dauer formation.
C. elegans mainly reproduces as a hermaphrodite. However, most Caenorhabditis worm species achieve this by means of cross-fertilization. These hermaphrodites are essentially females with the ability to self-fertilize, and they can also mate with males, but their numbers are typically relatively low. Hermaphrodites do not appear to be attracted to male C. elegans, but males are attracted to them [33]. The ascarosides ascr#2, 3, 4, and 8 (Table 1) not only play roles in regulating nematode development but also function as sex pheromones that are known to attract males [34][35]. They show synergistic effects, whereby a mixture of ascr#2, 3, and 4 is an effective male attractant at low concentrations. Ascr#3 attracts C. elegans males but repels hermaphrodites and can increase the lifespan of C. elegans. Ascr#8 is a strong male-specific attractant and shows synergy with ascr#2 and ascr#3. A mixture composed of ascr#3 and ascr#8 strongly attracts males at ultra-low concentrations, but at higher concentrations, it is repulsive to hermaphrodites [12][34][36]. The other two ascarosides with sex pheromone functions, ascr#6.1 (Table 1) and ascr#6.2 (Table 1), were identified by Paul as diastereomeric side-chain-hydroxylated ascarosides [34]. Ascr#10 (also called asc-C9) (Table 1) makes up the majority of the sex-specific milieu of ascarosides produced by male C. elegans. Ascr#3 has an α, β-unsaturated fatty acid moiety, whereas ascr#10 has the corresponding dihydro-derivative; such minor structural modifications deeply influence their signaling properties. The male pheromone ascr#10 strongly attracts hermaphrodite nematodes and shortens their lifespan [37][38][39]. It also can increase germline proliferation and physiological cell death [40] and change the reproductive physiology of hermaphroditism, such as by improving sperm orientation and increasing the number of reproductive precursor cells in adults [41][42][43]. Furthermore, Dong et al. conducted a comparative analysis of indole ascaroside signaling for 14 Caenorhabditis species. Icas#2 and icas#6.2 (Table 1) were isolated from hermaphrodites of C. briggsae and were found to synergistically attract conspecific males [44].
Panagrellus redivivus has an ecological niche similar to that of C. elegans; it has a free-living lifestyle but belongs to a different clade. In contrast with C. elegans, the virgin females of P. redivivus attract and are attracted by the males, but they do not attract the same sex [45]. The ascaroside biosynthesis in P. redivivus is highly sex-specific. The females of P. redivivus can excrete ascr#1, ascr#10, and bhas#10 (Table 1) [38]. The males of P. redivivus can excrete dhas#18 (Table 1) [38]. Ascr#1 can strongly attract males, but high concentrations of ascr#1 repel the females of P. redivivus. At high concentrations, bhas#10 and ascr#10 attract males rather than females. Dhas#18, which is a known dihydroxy derivative of ascr#18 secreted by males as well as an ascaroside with extensive functionality as a characteristic of its lipid-derived side chain, can strongly attract the females of P. redivivus. Bhas#18 (Table 1) is a precursor for dhas#18 synthesis, but its exact function is unclear [38].
Rhabditis sp. SB347 is a unique free-living dioecious species that is often used in the laboratory [46]. The females of SB347 produce ascr#1 and ascr#9 (Table 1), which function as sex pheromones. At femtomolar levels, ascr#1 and ascr#9 are strongly attracted to males, but not to hermaphrodites and female nematodes [47].
The dimorphism of the adults is an important feature of the life history of Globodera rostochiensis. Hermaphrodites attract males for mating by producing pheromones. Four fractions of the homospecific sex pheromone produced by virgin females, which were isolated using chromatography technology, were tested for their ability to attract male G. rostochiensis; only two of the fractions showed sex pheromone activity. Several weakly basic polar compounds constitute the sex pheromone of G. rostochiensis. The exact structure of its components is unclear [48].
The chemical structure diagram for sex pheromones secreted by nematodes is shown in Figure 2. This diagram shows ascaroside building blocks associated with the mating of different species of nematodes, including ascarylose sugars, variable-length fatty acids, and other modification groups.
Figure 2. Overview of the chemical structures of sex pheromones secreted by nematodes. Vanillic acid does not have an ascaroside structure, so it is not shown here, and its detailed structure is presented in Table 1.

3. Aggregation of Pheromones Secreted by Nematodes

C. elegans uses specifically modified forms of the ascarosides that contain indole units as highly effective aggregation pheromones. The indole ascarosides (ICASs) incorporate an L-tryptophan-derived indole-3-carboxylic acid group, which is linked to the four-position of the ascarylose moiety. An indole carboxy unit forms one indole derivative, and it is connected to an ascarylose bearing a nine-carbon unsaturated side chain identical to that found in the known ascr#3; this indole carboxy ascaroside is called “icas#3” (Table 1). The icas#3 occurs primarily by means of an expression protein in C. elegans, CEST-3, adding an IC group to the corresponding unmodified ascr#3 [49][50]. Icas#3 and icas#9 are relatively good attractants [49]. The 4-hydroxybenzoyl derivative of ascr#3 is called hbas#3 (Table 1). Hbas#3 was the first ascaroside with a 4-hydroxybenzoyl structure to be discovered. Hbas#3 strongly attracts C. elegans at low concentrations (10 fM), more effectively so than icas#3 and icas#9 [51]. Ascr#5 in combination with ascr#2 or ascr#3 may influence the aggregation of C. elegans adults; however, more in-depth research is needed on this topic [52].
See Figure 3 for a schematic overview of nematode pheromones related to their aggregation. It illustrates ascarylose sugars, fatty acids with variable lengths, and other modifications that form aggregation-related ascaroside species.
Figure 3. Overview of the chemical structure of the aggregation of pheromones secreted by nematodes.

4. Pheromones with Other Functions Secreted by Nematodes

The L1 larvae of C. elegans can specifically produce certain octopamine ascarosides, in which the ascarylose four-position is linked to a side chain derived from the succinylation of the neurotransmitter octopamine. The octopamine ascarosides osas#2 (Table 1), osas#9 (Table 1), and osas#10 (Table 1) play roles in dispersal [35]. Osas#9 is a pheromone that acts as a dispersal signal, especially in the case of a lack of food. Avoidance reactions to osas#9 require the G-protein-coupled receptor TYRA-2 [53]. With a continuous decrease in food, ascr#10 and osas#10 are converted to ascr#9, osas#9, and icas#9 [35].
Ascr#3 was found to regulate metabolism and avoidance behavior, it was defined as a population density pheromone. When food is scarce, ascr#3 causes hermaphrodites to have an avoidance effect [54][55][56]. When the ADF of a single sensory neuron is removed, both sexes are weakly rejected by the ascaroside ascr#3. Although ADF has functions in both sexes, ascr#3 is only detected in males, which is the result of the main sex regulator tra-1 [57]. A derivative of ascr#3 called mbas#3 (Table 1) was the first ascaroside discovered to have an (E)-2-methyl-2-butenoyl structure [51], and it acts as a dispersal signal in C. elegans, as well as having an antagonistic effect on the attractant characteristics of indole ascarides such as icas#3 and icas#9 [58].
C. elegans has shown a tendency to be attracted to a series of odorous substances, and with the passage of time, this tendency changes from attraction to dispersal [59][60]. This varied pheromone-mediated behavior is called olfactory plasticity, which depends on the population density [61]. However, the pheromone component that plays a major role in this process has not been identified. In addition, the pheromones released by injured conspecific nematodes are repellent to nematodes, and they may contain alarm pheromones. These alarm pheromones may not belong to the ascaroside class of pheromones [62]. However, their exact structure has not been identified.
The following Figure 4 displays the chemical structure diagram of function pheromones secreted by nematodes. This diagram shows the ascaroside building blocks associated with the functions of different species of nematodes, including ascarylose sugars, variable-length fatty acids, and other modification groups.
Table 1. Pheromones are secreted by nematodes.
Figure 4. Overview of the chemical structures of pheromones with other functions secreted by nematodes.

This entry is adapted from the peer-reviewed paper 10.3390/molecules28052409

References

  1. Kaletta, T.; Hengartner, M.O. Finding Function in Novel Targets: C. elegans as a Model Organism. Nat. Rev. Drug Discov. 2006, 5, 387–398.
  2. Cassada, R.C.; Russell, R.L. The Dauerlarva, A Post-Embryonic Developmental Variant of the Nematode Caenorhabditis elegans. Dev. Biol. 1975, 46, 326–342.
  3. Klass, M.; Hirsh, D. Non-Ageing Developmental Variant of Caenorhabditis elegans. Nature 1976, 260, 523–525.
  4. Schroeder, F.C. Modular Assembly of Primary Metabolic Building Blocks: A Chemical Language in C. elegans. Chem. Biol. 2015, 22, 7–16.
  5. Riddle, D.L.; Albert, P.S. Genetic and Environmental Regulation of Dauer Larva Development. In C. elegans II; Riddle, D.L., Blumenthal, T., Meyer, B.J., Priess, J.R., Eds.; Cold Spring Harbor Laboratory Press Copyright © 2023; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, USA, 1997.
  6. Kim, S.; Paik, Y.K. Developmental and Reproductive Consequences of Prolonged Non-aging Dauer in Caenorhabditis elegans. Biochem. Biophys. Res. Commun. 2008, 368, 588–592.
  7. Golden, J.W.; Riddle, D.L. A Pheromone Influences Larval Development in the Nematode Caenorhabditis elegans. Science 1982, 218, 578–580.
  8. Butcher, R.A. Small-Molecule Pheromones and Hormones Controlling Nematode Development. Nat. Chem. Biol. 2017, 13, 577–586.
  9. Jeong, P.Y.; Jung, M.; Yim, Y.H.; Kim, H.; Park, M.; Hong, E.; Lee, W.; Kim, Y.H.; Kim, K.; Paik, Y.K. Chemical Structure and Biological Activity of the Caenorhabditis elegans Dauer-Inducing Pheromone. Nature 2005, 433, 541–545.
  10. Butcher, R.A.; Fujita, M.; Schroeder, F.C.; Clardy, J. Small-Molecule Pheromones that Control Dauer Development in Caenorhabditis elegans. Nat. Chem. Biol. 2007, 3, 420–422.
  11. Cohen, S.M.; Wrobel, C.J.J.; Prakash, S.J.; Schroeder, F.C.; Sternberg, P.W. Formation and Function of Dauer Ascarosides in the Nematodes Caenorhabditis briggsae and Caenorhabditis elegans. G3 Bethesda 2022, 12, jkac014.
  12. Srinivasan, J.; Kaplan, F.; Ajredini, R.; Zachariah, C.; Alborn, H.T.; Teal, P.E.; Malik, R.U.; Edison, A.S.; Sternberg, P.W.; Schroeder, F.C. A Blend of Small Molecules Regulates Both Mating and Development in Caenorhabditis elegans. Nature 2008, 454, 1115–1118.
  13. Shimizu, T.; Sugiura, K.; Sakai, Y.; Dar, A.R.; Butcher, R.A.; Matsumoto, K.; Hisamoto, N. Chemical Signaling Regulates Axon Regeneration via the GPCR-Gqα Pathway in Caenorhabditis elegans. J. Neurosci. 2022, 42, 720–730.
  14. Park, J.; Oh, H.; Kim, D.Y.; Cheon, Y.; Park, Y.J.; Hwang, H.; Neal, S.J.; Dar, A.R.; Butcher, R.A.; Sengupta, P.; et al. CREB Mediates the C. elegans Dauer Polyphenism Through Direct and Cell-Autonomous Regulation of TGF-β Expression. PLoS Genet. 2021, 17, e1009678.
  15. Butcher, R.A.; Ragains, J.R.; Kim, E.; Clardy, J. A Potent Dauer Pheromone Component in Caenorhabditis elegans that Acts Synergistically with Other Components. Proc. Natl. Acad. Sci. USA 2008, 105, 14288–14292.
  16. Park, J.; Choi, W.; Dar, A.R.; Butcher, R.A.; Kim, K. Neuropeptide Signaling Regulates Pheromone-Mediated Gene Expression of a Chemoreceptor Gene in C. elegans. Mol. Cells 2019, 42, 28–35.
  17. Reilly, D.K.; McGlame, E.J.; Vandewyer, E.; Robidoux, A.N.; Muirhead, C.S.; Northcott, H.T.; Joyce, W.; Alkema, M.J.; Gegear, R.J.; Beets, I.; et al. Distinct Neuropeptide-Receptor Modules Regulate a Sex-Specific Behavioral Response to a Pheromone. Commun. Biol. 2021, 4, 1018.
  18. Hong, R.L.; Sommer, R.J. Pristionchus pacificus: A Well-Rounded Nematode. BioEssays 2006, 28, 651–659.
  19. Meyer, J.M.; Baskaran, P.; Quast, C.; Susoy, V.; Rödelsperger, C.; Glöckner, F.O.; Sommer, R.J. Succession and Dynamics of Pristionchus Nematodes and Their Microbiome During Decomposition of Oryctes Borbonicus on La Réunion Island. Environ. Microbiol. 2017, 19, 1476–1489.
  20. Sommer, R.J.; McGaughran, A. The Nematode Pristionchus pacificus as a Model System for Integrative Studies in Evolutionary Biology. Mol. Ecol. 2013, 22, 2380–2393.
  21. Bento, G.; Ogawa, A.; Sommer, R.J. Co-Option of the Hormone-Signalling Module Dafachronic Acid-DAF-12 in Nematode Evolution. Nature 2010, 466, 494–497.
  22. Bose, N.; Ogawa, A.; von Reuss, S.H.; Yim, J.J.; Ragsdale, E.J.; Sommer, R.J.; Schroeder, F.C. Complex Small-Molecule Architectures Regulate Phenotypic Plasticity in a Nematode. Angew. Chem. Int. Ed. Engl. 2012, 51, 12438–12443.
  23. Falcke, J.M.; Bose, N.; Artyukhin, A.B.; Rödelsperger, C.; Markov, G.V.; Yim, J.J.; Grimm, D.; Claassen, M.H.; Panda, O.; Baccile, J.A.; et al. Linking Genomic and Metabolomic Natural Variation Uncovers Nematode Pheromone Biosynthesis. Cell Chem. Biol. 2018, 25, 787–796.e12.
  24. Bose, N.; Meyer, J.M.; Yim, J.J.; Mayer, M.G.; Markov, G.V.; Ogawa, A.; Schroeder, F.C.; Sommer, R.J. Natural Variation in Dauer Pheromone Production and Sensing Supports Intraspecific Competition in Nematodes. Curr. Biol. 2014, 24, 1536–1541.
  25. Werner, M.S.; Claaßen, M.H.; Renahan, T.; Dardiry, M.; Sommer, R.J. Adult Influence on Juvenile Phenotypes by Stage-Specific Pheromone Production. iScience 2018, 10, 123–134.
  26. Ciche, T.A.; Kim, K.S.; Kaufmann-Daszczuk, B.; Nguyen, K.C.; Hall, D.H. Cell Invasion and Matricide during Photorhabdus luminescens Transmission by Heterorhabditis bacteriophora Nematodes. Appl. Environ. Microbiol. 2008, 74, 2275–2287.
  27. Ciche, T.A.; Ensign, J.C. For the Insect Pathogen Photorhabdus luminescens, Which End of a Nematode is Out? Appl. Environ. Microbiol. 2003, 69, 1890–1897.
  28. Ciche, T. The Biology and Genome of Heterorhabditis bacteriophora. WormBook 2007, 20, 1–9.
  29. Noguez, J.H.; Conner, E.S.; Zhou, Y.; Ciche, T.A.; Ragains, J.R.; Butcher, R.A. A Novel Ascaroside Controls the Parasitic Life Cycle of the Entomopathogenic Nematode Heterorhabditis bacteriophora. ACS Chem. Biol. 2012, 7, 961–966.
  30. Gomez-Diaz, C.; Benton, R. The Joy of Sex Pheromones. EMBO Rep. 2013, 14, 874–883.
  31. Wyatt, T.D. Pheromones and Signature Mixtures: Defining Species-Wide Signals and Variable Cues for Identity in Both Invertebrates and Vertebrates. J. Comp. Physiol. A Neuroethol. Sens. Neural. Behav. Physiol. 2010, 196, 685–700.
  32. Leighton, D.H.; Sternberg, P.W. Mating Pheromones of Nematoda: Olfactory Signaling with Physiological Consequences. Curr. Opin. Neurobiol. 2016, 38, 119–124.
  33. Simon, J.M.; Sternberg, P.W. Evidence of a Mate-Finding Cue in the Hermaphrodite Nematode Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 2002, 99, 1598–1603.
  34. Pungaliya, C.; Srinivasan, J.; Fox, B.W.; Malik, R.U.; Ludewig, A.H.; Sternberg, P.W.; Schroeder, F.C. A Shortcut to Identifying Small Molecule Signals that Regulate Behavior and Development in Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 2009, 106, 7708–7713.
  35. Artyukhin, A.B.; Yim, J.J.; Srinivasan, J.; Izrayelit, Y.; Bose, N.; von Reuss, S.H.; Jo, Y.; Jordan, J.M.; Baugh, L.R.; Cheong, M.; et al. Succinylated Octopamine Ascarosides and a New Pathway of Biogenic Amine Metabolism in Caenorhabditis elegans. J. Biol. Chem. 2013, 288, 18778–18783.
  36. Choe, A.; von Reuss, S.H.; Kogan, D.; Gasser, R.B.; Platzer, E.G.; Schroeder, F.C.; Sternberg, P.W. Ascaroside Signaling is Widely Conserved Among Nematodes. Curr. Biol. 2012, 22, 772–780.
  37. Izrayelit, Y.; Srinivasan, J.; Campbell, S.L.; Jo, Y.; von Reuss, S.H.; Genoff, M.C.; Sternberg, P.W.; Schroeder, F.C. Targeted Metabolomics Reveals a Male Pheromone and Sex-Specific Ascaroside Biosynthesis in Caenorhabditis elegans. ACS Chem. Biol. 2012, 7, 1321–1325.
  38. Choe, A.; Chuman, T.; von Reuss, S.H.; Dossey, A.T.; Yim, J.J.; Ajredini, R.; Kolawa, A.A.; Kaplan, F.; Alborn, H.T.; Teal, P.E.; et al. Sex-Specific Mating Pheromones in the Nematode Panagrellus redivivus. Proc. Natl. Acad. Sci. USA 2012, 109, 20949–20954.
  39. Shi, C.; Runnels, A.M.; Murphy, C.T. Mating and Male Pheromone Kill Caenorhabditis Males Through Distinct Mechanisms. Elife 2017, 6, 23493.
  40. Aprison, E.Z.; Dzitoyeva, S.; Angeles-Albores, D.; Ruvinsky, I. A Male Pheromone that Improves the Quality of the Oogenic Germline. Proc. Natl. Acad. Sci. USA 2022, 119, e2015576119.
  41. Aprison, E.Z.; Ruvinsky, I. Sex Pheromones of C. elegans Males Prime the Female Reproductive System and Ameliorate the Effects of Heat Stress. PLoS Genet. 2015, 11, e1005729.
  42. Aprison, E.Z.; Ruvinsky, I. Sexually Antagonistic Male Signals Manipulate Germline and Soma of C. elegans Hermaphrodites. Curr. Biol. 2016, 26, 2827–2833.
  43. Aprison, E.Z.; Ruvinsky, I. Counteracting Ascarosides Act through Distinct Neurons to Determine the Sexual Identity of C. elegans Pheromones. Curr. Biol. 2017, 27, 2589–2599.e3.
  44. Dong, C.; Dolke, F.; von Reuss, S.H. Selective MS Screening Reveals a Sex Pheromone in Caenorhabditis briggsae and Species-Specificity in Indole Ascaroside Signalling. Org. Biomol. Chem. 2016, 14, 7217–7225.
  45. Balakanich, S.; Samoiloff, M.R. Development of Nematode Behavior: Sex Attraction Among Different Strains of the Free-Living Panagrellus redivivus. Can. J. Zool. 1974, 52, 835–845.
  46. Chaudhuri, J.; Kache, V.; Pires-daSilva, A. Regulation of Sexual Plasticity in a Nematode that Produces Males, Females, and Hermaphrodites. Curr. Biol. 2011, 21, 1548–1551.
  47. Chaudhuri, J.; Bose, N.; Tandonnet, S.; Adams, S.; Zuco, G.; Kache, V.; Parihar, M.; von Reuss, S.H.; Schroeder, F.C.; Pires-daSilva, A. Mating Dynamics in a Nematode with Three Sexes and Its Evolutionary Implications. Sci. Rep. 2015, 5, 17676.
  48. Riga, E.; Holdsworth, D.R.; Perry, R.N.; Barrett, J.; Johnston, M.R. Electrophysiological Analysis of the Response of Males of the Potato Cyst Nematode, Globodera rostochiensis, to Fractions of Their Homospecific Sex Pheromone. Parasitology 1997, 115 Pt 3, 311–316.
  49. Srinivasan, J.; von Reuss, S.H.; Bose, N.; Zaslaver, A.; Mahanti, P.; Ho, M.C.; O’Doherty, O.G.; Edison, A.S.; Sternberg, P.W.; Schroeder, F.C. A Modular Library of Small Molecule Signals Regulates Social Behaviors in Caenorhabditis elegans. PLoS Biol. 2012, 10, e1001237.
  50. Faghih, N.; Bhar, S.; Zhou, Y.; Dar, A.R.; Mai, K.; Bailey, L.S.; Basso, K.B.; Butcher, R.A. A Large Family of Enzymes Responsible for the Modular Architecture of Nematode Pheromones. J. Am. Chem. Soc. 2020, 142, 13645–13650.
  51. von Reuss, S.H.; Bose, N.; Srinivasan, J.; Yim, J.J.; Judkins, J.C.; Sternberg, P.W.; Schroeder, F.C. Comparative Metabolomics Reveals Biogenesis of Ascarosides, a Modular Library of Small-Molecule Signals in C. elegans. J. Am. Chem. Soc. 2012, 134, 1817–1824.
  52. Macosko, E.Z.; Pokala, N.; Feinberg, E.H.; Chalasani, S.H.; Butcher, R.A.; Clardy, J.; Bargmann, C.I. A Hub-and-Spoke Circuit Drives Pheromone Attraction and Social Behaviour in C. elegans. Nature 2009, 458, 1171–1175.
  53. Chute, C.D.; DiLoreto, E.M.; Zhang, Y.K.; Reilly, D.K.; Rayes, D.; Coyle, V.L.; Choi, H.J.; Alkema, M.J.; Schroeder, F.C.; Srinivasan, J. Co-option of Neurotransmitter Signaling for Inter-Organismal Communication in C. elegans. Nat. Commun. 2019, 10, 3186.
  54. Hussey, R.; Stieglitz, J.; Mesgarzadeh, J.; Locke, T.T.; Zhang, Y.K.; Schroeder, F.C.; Srinivasan, S. Pheromone-Sensing Neurons Regulate Peripheral Lipid Metabolism in Caenorhabditis elegans. PLoS Genet. 2017, 13, e1006806.
  55. Luo, J.; Portman, D.S. Sex-Specific, Pdfr-1-Dependent Modulation of Pheromone Avoidance by Food Abundance Enables Flexibility in C. elegans Foraging Behavior. Curr. Biol. 2021, 31, 4449–4461.e4.
  56. Jang, H.; Kim, K.; Neal, S.J.; Macosko, E.; Kim, D.; Butcher, R.A.; Zeiger, D.M.; Bargmann, C.I.; Sengupta, P. Neuromodulatory State and Sex Specify Alternative Behaviors Through Antagonistic Synaptic Pathways in C. elegans. Neuron 2012, 75, 585–592.
  57. Fagan, K.A.; Luo, J.; Lagoy, R.C.; Schroeder, F.C.; Albrecht, D.R.; Portman, D.S. A Single-Neuron Chemosensory Switch Determines the Valence of a Sexually Dimorphic Sensory Behavior. Curr. Biol. 2018, 28, 902–914.e5.
  58. Zhang, Y.K.; Sanchez-Ayala, M.A.; Sternberg, P.W.; Srinivasan, J.; Schroeder, F.C. Improved Synthesis for Modular Ascarosides Uncovers Biological Activity. Org. Lett. 2017, 19, 2837–2840.
  59. Dal Bello, M.; Perez-Escudero, A.; Schroeder, F.C.; Gore, J. Inversion of Pheromone Preference Optimizes Foraging in C. elegans. Elife 2021, 10, 58144.
  60. Nuttley, W.M.; Atkinson-Leadbeater, K.P.; Van Der Kooy, D. Serotonin Mediates Food-Odor Associative Learning in the Nematode Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 2002, 99, 12449–12454.
  61. Yamada, K.; Hirotsu, T.; Matsuki, M.; Butcher, R.A.; Tomioka, M.; Ishihara, T.; Clardy, J.; Kunitomo, H.; Iino, Y. Olfactory Plasticity is Regulated by Pheromonal Signaling in Caenorhabditis elegans. Science 2010, 329, 1647–1650.
  62. Zhou, Y.; Loeza-Cabrera, M.; Liu, Z.; Aleman-Meza, B.; Nguyen, J.K.; Jung, S.K.; Choi, Y.; Shou, Q.; Butcher, R.A.; Zhong, W. Potential Nematode Alarm Pheromone Induces Acute Avoidance in Caenorhabditis elegans. Genetics 2017, 206, 1469–1478.
  63. Greene, J.S.; Brown, M.; Dobosiewicz, M.; Ishida, I.G.; Macosko, E.Z.; Zhang, X.; Butcher, R.A.; Cline, D.J.; McGrath, P.T.; Bargmann, C.I. Balancing Selection Shapes Density-Dependent Foraging Behaviour. Nature 2016, 539, 254–258.
  64. Hong, M.; Ryu, L.; Ow, M.C.; Kim, J.; Je, A.R.; Chinta, S.; Huh, Y.H.; Lee, K.J.; Butcher, R.A.; Choi, H.; et al. Early Pheromone Experience Modifies a Synaptic Activity to Influence Adult Pheromone Responses of C. elegans. Curr. Biol. 2017, 27, 3168–3177.e3.
  65. Kaplan, F.; Perret-Gentil, A.; Giurintano, J.; Stevens, G.; Erdogan, H.; Schiller, K.C.; Mirti, A.; Sampson, E.; Torres, C.; Sun, J.; et al. Conspecific and Heterospecific Pheromones Stimulate Dispersal of Entomopathogenic Nematodes During Quiescence. Sci. Rep. 2020, 10, 5738.
  66. Kaplan, F.; Alborn, H.T.; von Reuss, S.H.; Ajredini, R.; Ali, J.G.; Akyazi, F.; Stelinski, L.L.; Edison, A.S.; Schroeder, F.C.; Teal, P.E. Interspecific Nematode Signals Regulate Dispersal Behavior. PLoS ONE 2012, 7, e38735.
  67. Hartley, C.J.; Lillis, P.E.; Owens, R.A.; Griffin, C.T. Infective Juveniles of Entomopathogenic Nematodes (Steinernema and Heterorhabditis) Secrete Ascarosides and Respond to Interspecific Dispersal Signals. J. Invertebr. Pathol. 2019, 168, 107257.
  68. Zhao, M.; Wickham, J.D.; Zhao, L.; Sun, J. Major Ascaroside Pheromone Component asc-C5 Influences Reproductive Plasticity Among Isolates of the Invasive Species Pinewood Nematode. Integr. Zool. 2021, 16, 893–907.
  69. Meng, J.; Wickham, J.D.; Ren, W.; Zhao, L.; Sun, J. Species Displacement Facilitated by Ascarosides Between Two Sympatric Sibling Species: A Native and Invasive Nematode. J. Pest Sci. 2020, 93, 1059–1071.
  70. Manosalva, P.; Manohar, M.; von Reuss, S.H.; Chen, S.; Koch, A.; Kaplan, F.; Choe, A.; Micikas, R.J.; Wang, X.; Kogel, K.H.; et al. Conserved Nematode Signalling Molecules Elicit Plant Defenses and Pathogen Resistance. Nat. Commun. 2015, 6, 7795.
  71. Kong, X.; Huang, Z.; Gu, X.; Cui, Y.; Li, J.; Han, R.; Jin, Y.; Cao, L. Dimethyl Sulfoxide and Ascarosides Improve the Growth and Yields of Entomopathogenic Nematodes in Liquid Cultures. J. Invertebr. Pathol. 2022, 193, 107800.
  72. Wang, J.; Cao, L.; Huang, Z.; Gu, X.; Cui, Y.; Li, J.; Li, Y.; Xu, C.; Han, R. Influence of the Ascarosides on the Recovery, Yield and Dispersal of Entomopathogenic Nematodes. J. Invertebr. Pathol. 2022, 188, 107717.
  73. Butcher, R.A.; Ragains, J.R.; Clardy, J. An Indole-Containing Dauer Pheromone Component with Unusual Dauer Inhibitory Activity at Higher Concentrations. Org. Lett. 2009, 11, 3100–3103.
  74. Ludewig, A.H.; Artyukhin, A.B.; Aprison, E.Z.; Rodrigues, P.R.; Pulido, D.C.; Burkhardt, R.N.; Panda, O.; Zhang, Y.K.; Gudibanda, P.; Ruvinsky, I.; et al. An Excreted Small Molecule Promotes C. elegans Reproductive Development and Aging. Nat. Chem. Biol. 2019, 15, 838–845.
  75. Jaffe, H.; Huettel, R.N.; Demilo, A.B.; Hayes, D.K.; Rebois, R.V. Isolation and Identification of a Compound from Soybean Cyst Nematode, Heterodera glycines, with Sex Pheromone Activity. J. Chem. Ecol. 1989, 15, 2031–2043.
  76. Meyer, S.L.; Johnson, G.; Dimock, M.; Fahey, J.W.; Huettel, R.N. Field Efficacy of Verticillium lecanii, Sex Pheromone, and Pheromone Analogs as Potential Management Agents for Soybean Cyst Nematode. J. Nematol. 1997, 29, 282–288.
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