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Geochemistry & Geophysics
Several studies set out to explain the presence of high proportions of photooxidation products of cis-vaccenic acid (generally considered to be of bacterial origin) in marine environments.
- attached bacteria
- phytodetritus
- oxidative damage
- singlet oxygen transfer
- solar irradiance
1. Introduction
Phototrophic organisms carry out photosynthetic reactions that convert chlorophyll into a singlet excited state (1Chl) under the action of light. A small fraction of the 1Chl formed can undergo intersystem crossing to produce a longer-living triplet state, 3Chl [1]. 3Chl can directly damage unsaturated membrane components through type I reactions (i.e., involving radicals) [1], but it can also react with ground-state oxygen (3O2) to generate singlet oxygen (1O2) and, although to a lesser extent, superoxide ions (O2−•). In living cells, the toxic effects of 3Chl and 1O2 are limited by endogenous quenchers or scavengers (carotenoids, tocopherols, ascorbic acid, superoxide dismutase enzymes) [2,3], but this is not the case during cell senescence or cell death. When cells senesce, the slowdown of 1Chl consumption in the photosynthetic reactions accelerates the conversion of 1Chl into 3Chl and, thus, into 1O2, which then saturates the photoprotective system and ultimately causes photodamage [4,5]. During the senescence of phototrophic organisms, type II photosensitized oxidation processes that mainly involve 1O2 strongly damage unsaturated membrane lipids. The very high reactivity of 1O2 with unsaturated compounds results from its strong electrophilicity and the lack of spin restriction that normally hinders 3O2 reacting with unsaturated compounds [6].
Type II photosensitized oxidation processes act intensively on several unsaturated lipids, including chlorophyll itself but also unsaturated fatty acids, sterols, some n-alkenes, some highly branched isoprenoid (HBI) alkenes, carotenoids, and tocopherols (for review, see [5,7,8,9]). Type II photosensitized oxidation of monounsaturated fatty acids (MUFAs) involves a direct reaction of 1O2 with the carbon–carbon double bond via a concerted ‘ene’ addition [10], leading to the formation of hydroperoxides at each end of the original double bond [11] (see Figure 1 showing oxidation of cis-vaccenic acid). These hydroperoxides, which possess an allylic trans-double bond can subsequently undergo stereoselective radical allylic rearrangement and afford two other isomers with a trans-double bond [11,12] (Figure 1). Type II photosensitized oxidation of MUFAs, thus, leads to the formation of four isomeric allylic hydroperoxyacids, which are generally converted to their corresponding hydroxyacids by NaBH4 reduction for quantification in natural samples [13]. This approach has been used to detect high levels of photoproducts of phytoplanktonic MUFAs in several marine particulate and sediment samples (for a recent review see [9]).
Figure 1. Type II photosensitized oxidation of cis-vaccenic acid and subsequent allylic rearrangement of the hydroperoxyacids formed.
Surprisingly, photoproducts of octadec-11(cis)-enoic acid (cis-vaccenic acid) have also been detected in a number of different zones of the oceans [7,8,9,14,15] and sometimes in similar or even higher proportions (relative to the parent acid) than photoproducts of phytoplanktonic MUFAs. Cis-vaccenic acid has been proposed as a useful biological marker for bacteria in the marine environment, based on its higher relative concentrations in bacteria than in other organisms [16,17,18,19,20]. However, given that heterotrophic bacteria lack photosynthetic system, the presence of high proportions of these photoproducts was a very surprising finding.
2. Potential Sources of Photoproducts of cis-Vaccenic Acid in Oceans
2.1. Photooxidation of Aerobic Anoxygenic Phototrophic Bacteria (AAPB)
Aerobic anoxygenic phototrophic bacteria (AAPB) are an important group of microorganisms inhabiting the euphotic zones of oceans and freshwater or saline lakes [21]. They do not form a monophyletic clade but are widely distributed within Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria classes [22,23]. These bacteria perform a heterotrophic metabolism because they require organic carbon for growth, but they can also use photosynthesis as a supplemental energy source [24]. Due to their ability to obtain extra energy from light, AAPB can have a higher impact on the degradation of organic matter than strict heterotrophs [25]. It has been previously demonstrated that AAPB are widely distributed in the open ocean [26,27,28]. The induction of type II photosensitized oxidation processes in these organisms, which contain bacteriochlorophyll (which is a highly efficient photosensitizer [29]) and high proportions of cis-vaccenic acid in their membranes [30,31] could, therefore, be at the origin of the presence of cis-vaccenic acid photoproducts in the samples investigated.
To test this hypothesis, senescent cells of Erythrobacter sp. strain NAP1 and Roseobacter sp. strain COL2P were exposed to photosynthetically available radiation (PAR) [32]. The profile of oxidation products of cis-vaccenic acid obtained after exposure to PAR did not correspond to the profile observed in situ. In fact, as we have seen previously, the attack of cis-vaccenic acid by 1O2-mediated processes only produces trans allylic hydroperoxyacids (Figure 1), whereas the irradiation of senescent AAPB results in the formation of high proportions of cis isomers, which are characteristic of radical oxidation processes [33]. Previous research shows that the 1O2 produced in senescent phytoplankton cells (for a review see [9]), but also in purple sulfur bacteria (Thiohalocapsa halophila and Halochromatium salexigens) [34] by type II chlorophyll or bacteriochlorophyll-photosensitized processes intensively attacks the phytyl side-chain of these pigments, affording a specific photoproduct (3-methylidene-7,11,15-trimethylhexadecan-1,2-diol) [8]. The fact that this compound is not found after irradiation of AAPB confirms that 1O2 is only very weakly produced during the senescence of these organisms. The oxidation of cis-vaccenic acid in AAPB, thus, appears to involve radical degradation processes and is clearly not at the origin of the presence of high proportions of cis-vaccenic acid photooxidation products resulting from 1O2-mediated processes in the oceans.
2.2. Transfer of Photochemically Produced 1O2 from Senescent Phytoplanktonic Cells to Their Attached Heterotrophic Bacteria
Another explanation for the unexpected presence of cis-vaccenic acid photoproducts in marine systems could be 1O2 transfer in attached heterotrophic bacteria during the senescence of phytoplankton. Bacteria are known to colonize phytoplankton-derived particles [35,36]. Figure 2 shows an example of the close association between bacteria and phytoplankton. Senescent phytoplanktonic cells provide hydrophobic micro-environments in which the lifetime and the potential diffusive distance of 1O2 could be long enough to induce type II photosensitized oxidation processes in attached bacteria. Indeed, the intracellular sphere of activity of 1O2 has recently been re-evaluated [37] and the radius of this sphere of activity from the point of production appeared to be larger than previously thought. It is estimated at between 155 and 340 nm [37,38,39], which is a large-enough distance to allow 1O2 to cross the cell membranes of phytoplanktonic cells (thickness ranging from 70 to 80 nm) [37,40,41] and, thus, reach attached bacteria.
Figure 2. Scanning electron microscopy image of a strain of the diatom Skeletonema costatum colonized with heterotrophic bacteria (adapted from [32]).
In an attempt to validate this hypothesis, Rontani et al. [32] performed parallel experiments using PAR lamps to irradiate: (i) dead axenic cells of the diatom Skeletonema costatum strain CS-181, (ii) dead axenic cells of the same diatom contaminated with a heterotrophic bacterial community, and (iii) the heterotrophic bacterial community (that had been used as the contaminant community) alone [37,38]. The results obtained showed that the photodegradation of cis-vaccenic acid from heterotrophic bacteria was more than two orders of magnitude faster in heterotrophic bacteria attached to phytoplanktonic cells than in the bacterial community alone. Interestingly, the profile of the cis-vaccenic oxidation products obtained matched perfectly to the profile detected in situ. This means that 1O2 produced by type II photosensitized oxidation processes in senescing phytoplanktonic cells can migrate across the membranes to the attached heterotrophic bacteria and go on to induce oxidative damage in them. These results were later confirmed by Petit et al. [42], who show that the photodegradation state of cis-vaccenic acid from bacteria attached to phytodetritus is strongly correlated with the photodegradation state of their chlorophyll. Photodegradation of heterotrophic bacteria attached to senescent phytoplanktonic cells, thus, emerges as the likely source of the oxidation products of cis-vaccenic acid detected in situ. This assumption is well-supported by the fact that attached bacteria are more likely to become part of the sinking material (which also shows strong photooxidation of cis-vaccenic acid) [7,13] than free-living AAPB.
This entry is adapted from the peer-reviewed paper 10.3390/microorganisms11061565
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