|
| Anthocyanins (cyanidin 3-O-glucoside, pelargonidin 3-O-glucoside cyanidin-malonyl-glucoside and pelargonidin-malonyl-glucoside); procyanidins, ellagitannins, ellagic acid and flavonol derivatives
|
HPLC-DAD, LC-ESI-MS
|
[9][ |
| Procyanidins, ellagitannins, ellagic acid and flavonol derivatives |
|
HPLC-DAD, LC-ESI-MS
|
[9][26]
|
Fragaria × ananassa
|
Fruits
|
Anthocyanins (pelargonidin-3-glucoside, pelargonidin-3-rutinoside, cyanidin-3-rutinoside, pelargonidin-3,5-diglucoside, pelargonidin-3-(6-acetyl)-glucoside, 5-carboxypyranopelargonidin-3-glucoside, delphinidin-3-glucoside, peonidin-3-glucoside, cyanidin-3-galactoside), p-hydroxybenzoic acid, (+)-catechin, ellagic acid, p-coumaric acid, quercetin glucoside
|
LC-MS/MS, HPLC-UV/Vis
|
[ | 26]
|
Phenolic acids
|
Fragaria x ananassa Osogrande var. frozen fruits
|
4-coumaric acid, p-hydroxybenzoic acid, ferulic acid, vanillic acid, sinapic acid
|
[7][15]
|
| Acetone (80%) extraction
|
DPPH, FRAP
|
DPPH: 11.91–12.83 μmol BHT eq./g FW; best results for ripe fruits FRAP: 27.37–36.75 μmol FS eq./g FW; best results for green fruits
|
Total phenolic content, vitamin C
|
[12][28]
|
Vitamins
|
Fragaria x ananassa Camino Real var. frozen fruits |
Vitamin C, vitamin B9
|
|
Acetone (80%) extraction
[6][14]
|
| DPPH, FRAP |
|
DPPH: 9.75–12.01 μmol BHT eq./g FW, FRAP: 24.13–28.49 μmol FS eq./g FW (best results for pink fruits)
|
Anti-inflammatory, wound healing
|
Fragaria x ananassa var. San Andreas fruits |
| Total phenolic content, vitamin C |
|
|
Ultrasound-assisted extraction, acidified meth.: aq. (80:20); separation of different fractions
|
MTT assay, ROS, NO levels, effects on inflammatory markers and on skin fibroblast migration
|
ROS reduction, suppression of IL-1β, IL-6 and iNOS gene expressions; enhanced skin fibroblast migration
[12][28]
|
Polyphenolic compounds, especially anthocyanins
|
[55][84]
|
Minerals
|
Mn, K, Mg, P, Ca
|
[3][11]
|
F. vesca leaves
|
Anti-microbial
|
Methanol, ultrasounds extraction
|
Fragaria vesca leaves and roots
DPPH, FRAP
|
|
Centrifugation extraction with meth.: aq. (80:20)
DPPH: IC50 = 13.46 mg/L; FRAP: 0.878 mmol Fe2+/g DW
|
Disc diffusion assay
[13][18]
|
Total phenols, total tannins
|
|
6–9 mm inhibition zones for leaves, 5–9 mm for roots (depending on S. aureus strain)
[37][72]
|
Phenolic compounds
|
[47][85]
|
Others
|
Sugars (glucose, fructose, and sucrose), fibers
|
Fragaria × ananassa
|
Fruits, different cultivars (27) and ripening stages
|
F. vesca roots, wild-growing
|
Hydromethanolic extraction, infusion, decoction
|
Phenolic compounds (multiple classes, including anthocyanins, flavanols and ellagitannins); composition dependent on cultivar, cinnamic acid conjugates and anthocyanins levels increased with the ripening stage
|
HPLC-DAD-MS
|
DPPH, FRAP, β-Carotene bleaching inhibition, TBARS
|
Anti-microbial
|
Fragaria vesca leaves
|
| IC50, mg/L: DPPH—50.03/50.56/50.62; FRAP—40.98/44.78/49.23; β-C bleaching—116.26/44.88/66.10; TBARS—35.76/4.76/6.14
|
|
Hydroalcoholic extraction, separation
[14 |
Disc diffusion assay
][29]
|
| Total phenolics, total flavan-3-ols, total dihydroflavonols, |
|
| Good inhibition potential at 25 mg/mL, better effect for the ellagitannin-enriched fraction
|
[38][73]
|
Ellagitannins
|
[48][86]
|
Fragaria × ananassa, F. vesca
|
Fruits
|
Quercetin and isorhamnetin glycosides (higher levels in wild strawberry)
|
HPLC-DAD, LC-ESI-MS
|
F. vesca roots, commercial
|
Anti-allergenic
|
Hydromethanolic extraction, infusion, decoction
|
Fragaria x ananassa var.
DPPH, FRAP, β-Carotene bleaching inhibition, TBARS
|
Minomusume fruits
IC50, mg/L: DPPH—68.89/255.81/51.32; FRAP—327.75/78.99/67.92; β-C bleaching—68.34/23.44/114.67; TBARS—6.69/24.25/10.62 |
[15][30]
|
|
|
| Total phenolics, total flavan-3-ols, total dihydroflavonols, |
|
Methanol fraction of fruits juice (obtained by squeezing)
|
Antigen-stimulated degranulation in RBL-2H3 cells
|
degranulation suppression (95–60% inhibition for linocinnamarin, cinnamic acid, chrysin, kaempferol, trans-tiliroside)
[38][73]
|
|
Best results - phenylpropanoid glycoside
|
[49 |
Fragaria × ananassa, F. vesca
|
Fruits, different cultivars
|
Volatile esters (including ethyl acetate, hexyl acetate, methyl butanoate, ethyl butanoate, hexyl butanoate, methyl hexanoate, ethyl hexanoate, hexyl hexanoate); higher levels in cultivated strawberries.
|
] | [ | 87 | ] |
|
Fragaria × ananassa var. Amaou,
|
Anti-diabetic
| GC-MS |
|
Fragaria x ananassa var. |
[16][19]
|
fruits, at different ripening stage
|
Ethanol or water room temperature extraction
|
Falandi fruits
Modified ABTS assay
|
|
Compounds isolated from eth. extractsEthanol: 150.5/151.9; water: 227.2/189.4 (red/green fruits) μmol TE/100 g FW
|
|
α-glucosidase inhibitory activity
Total phenolic content
|
IC50 values better than the positive control (acarbose) for nine compounds (537.43 to 25.39 μM)
[21][35]
|
Individual compounds
|
[24][20]
|
F. vesca
|
Fruits, two different cultivars
|
Anthocyanins (cyanidin 3-O |
Fragaria × ananassa var. Amaou calyx (red and green)
|
| -glucoside, pelargonidin 3- | O |
Anti-obesity, anti-allergy, skin-lightening
Ethanol or water room temperature extraction
|
Fragaria ×ananassa var. Amaou, entire plant (red fruit, green fruit, red calyx, green calyx, flower, leaf, stolon, stolon leaf, stem, crown and root)
-glucoside, peonidin 3-O-glucoside, cyanidin 3-O-malonylglucoside, pelargonidin 3-O-malonylglucoside, peonidin 3-O-malonylglucoside), dihydroflavonol and flavonols (taxifolin 3-O-arabinoside, kaempferol 3-O-glucoside, quercetin 3-O-glucoside, quercetin-acetylhexoside, kaempferol 3-O-acetylhexosides), flavan-3-ols and proanthocyanidins (catechin, B type proanthocyanidin dimers, trimers, and tetramers), ellagic acid and derivatives (glycosylated, methyl pentoside, methylellagic acid methyl pentoside, ellagitannins), other compounds (benzoic acid, ferulic acid hexose derivative, citric acid, furaneol glucoside)
|
Modified ABTS assay |
Eth. or aq. room temperature extraction
HPLC-DAD
|
|
Ethanol: 241.1/1239.9; water: 1716.6/577.7 μmol TE/100 g FW (red/green calyx)
|
Anti-lipase assay, adipocyte differentiation inhibition assay, melanogenesis inhibition assay, β-hexosaminidase inhibition assay, tyrosinase inhibition assay
[17][31]
|
| Total phenolic content |
|
| Crown, stolon leaf and flowers extracts exhibited the highest effects |
[21][35]
|
|
Total phenolic content
|
[21][35]
|
Fragaria × ananassa, F. vesca
|
Fragaria × ananassa var. Amaou flower
|
Fruits
|
|
Antihyperuricemic
| Ethanol or water room temperature extraction |
Fragaria x ananassa cv. Tochiotome leaves
Anthocyanins (cyanidin, pelargonidin), cyanidin glycosides (cyanidin 3-glucoside, cyanidin 3-arabinoside, cyanidin 3-sambubioside, delphinidin 3-galactoside, delphinidin 3-glucoside, delphinidin 3-malonylglucoside); higher levels of cyanidin glycosides in wild species
|
|
Modified ABTS assay
|
Supercritical CO2 extraction with different entrainers
HPLC-DAD
|
|
4234.4/387.5 μmol TE/100 g FW (ethanol/water)
[18][32]
|
| Total phenolic content |
|
| [ | 21][35]
|
Uric acid production in AML12 hepatocytes
|
Reduction of uric acid at 100 mg/mL (96 mmol/2 h/mg protein), compared with the control (16,096 mmol/2 h/mg protein)
|
Kaempferol, quercetin
|
[40][10]
|
F. vesca
|
Leaves
|
Fragaria × ananassa var. |
| Ellagitannins (sanguiin H-2 isomer, sanguiin H-10 isomer, sanguiin H-6/agrimoniin/lambertianin A isomer, castalagin/vescalagin isomer, sanguiin H-10 isomer, sanguiin H-2 isomer, casuarictin/potentillin isomer)
|
Amaou leaves
|
Cytotoxic, anti-proliferative
|
Fragaria x ananassa |
| LC-PDA-ESI-MS
|
Ethanol or water room temperature extraction
|
fruits
Modified ABTS assay
|
Meth. extraction
2401.7/241.1 μmol TE/100 g FW (ethanol/water)
[ |
Ex vivo: cell viability assay; in vivo: developing tumor size determination19][33]
|
| Total phenolic content |
|
| [ | 21][35]
|
|
Cytotoxic on cancer cells, blocked the proliferation of tumor cells
|
Phenolic compounds
|
[56][89]
|
Fragaria × ananassa
|
Fruits, different cultivars and production years
|
Vitamin C, anthocyanins (pelargonidin 3-glucoside, cyanidin 3-glucoside, pelargonidin 3-rutinoside), ellagic acid; strongly dependent on the cultivar and production year
|
HPLC-UV/Vis
|
[20][34]
|
Fragaria × ananassa var. Amaou stolon
|
Ethanol or water room temperature extraction
|
Modified ABTS assay |
Antineoplastic
|
Fragaria x ananassa var. Pajaro fruits
|
|
Acidified hydro-eth. extraction
1089.4/1856.7 μmol TE/100 g FW (ethanol/water)
|
Transglutaminase assay and polyamine detection, immunoblot analysis
Total phenolic content
|
reduction of cell proliferation, lowering of the intracellular levels of polyamine, enhancement of tissue transglutaminase activity
[21][35]
|
Anthocyanins
|
[57][90]
|
Fragaria × ananassa
|
|
Fragaria × ananassa var. Amaou stem | Fruits, at different ripening stage
|
Vitamin C, pelargonidin-3-rutinoside, ellagic acid, cyanidin-3-glucoside, quercetin (red fruits), neochlorogenic, pelargonidin-3-glucoside, pelargonidin-3-rutinoside, epicatechin, quercetin-3-β-d-glucoside, ellagic acid (green fruits)
|
LC-ESI-TOF
|
|
Ethanol or water room temperature extraction
|
Modified ABTS assay
[21][35]
|
| 1338.6/1123.1 μmol TE/100 g FW (ethanol/water) |
|
| Total phenolic content
|
[21][35]
|
Fragaria × ananassa
|
Fragaria × ananassa var. Amaou crown
|
Calyx (red and green)
|
|
Ethanol or water room temperature extraction
Quercetin-3-β-d-glucoside, ellagic acid, kaempferol-3-O-glucoside, vitamin C (red), catechin, quercetin-3-β-d-glucoside, ellagic acid (green)
|
Modified ABTS assay
LC-ESI-TOF
|
|
6213.3/128.7 μmol TE/100 g FW (ethanol/water)
[21][35]
|
| Total phenolic content |
|
| [ | 21][35]
|
Fragaria × ananassa
|
|
Fragaria × ananassa var. Amaou root | Flower
|
|
Ethanol or water room temperature extraction
Catechin, quercetin-3-β-d-glucoside, ellagic acid, kaempferol-3-O-glucoside, vitamin C
|
Modified ABTS assay
LC-ESI-TOF
|
|
253.1/69.2 μmol TE/100 g FW (ethanol/water)
[21][35]
|
| Total phenolic content |
|
| [ | 21][35]
|
Fragaria × ananassa
|
Leaf
|
Procyanidin dimer and trimer, catechin, quercetin-3-β- |
F. vesca vegetative parts (leaves and stems), wild-growing
|
Hydromethanolic and aqueous extracts; wild-growing infusion microencapsulated in alginate and incorporated in k-carrageenan gelatine
| d-glucoside, vitamin C, ellagic acid
|
LC-ESI-TOF
|
[ |
DPPH, FRAP, β-Carotene bleaching inhibition, TBARS
|
IC50, mg/L: DPPH—123.67/86.17/109.10; FRAP—81.40/62.36/77.28; β-C bleaching—56.71/12.34/13.40; TBARS—12.63/3.12/5.03 (hydromethanolic/infusion/decoction); Final formulation (mg/mL)—DPPH—2.74; FRAP = 1.23
21][35]
|
| Total phenolics, total flavan-3-ols, total dihydroflavonols, |
|
| [ | 39][74 |
Fragaria × ananassa
|
Stolon
|
Neochlorogenic, procyanidin dimer, catechin, quercetin-3-β-d-glucoside, ellagic acid, vitamin C, kaempferol-3-O-glucoside
|
LC-ESI-TOF
|
[21][35]
|
] |
|
F. vesca vegetative parts (leaves and stems), commercial
|
Hydromethanolic and aqueous extracts
|
DPPH, FRAP, β-Carotene bleaching inhibition, TBARS
|
IC50, mg/L: DPPH—139.33/121.94/118.89; FRAP—324.49/91.88/88.20; β-C bleaching—388.90/76.41/69.98; TBARS—24.36/23.07/17.52 (hydromethanolic/infusion/decoction).
|
Total phenolics, total flavan-3-ols, total dihydroflavonols,
|
[39][74]
|
Fragaria × ananassa
|
Fragaria x ananassa cv. Falandi fruit
|
Stem
|
|
22 compounds isolated from ethanolic extracts
Procyanidin dimer, catechin, ferulic acid, quercetin-3-β-d-glucoside, ellagic acid
|
LC-ESI-TOF
|
ABTS, DPPH, FRAP
|
Best results (IC50): ABTS—4.42 μM kaempferol 3-(6-methylglucuronide); DPPH—32.12 μM quercetin 3-(6-methylglucuronide); FRAP—0.05 mmol/g—urolignoside.
[21][35]
|
| Individual compounds |
|
| [ | 24][20]
|
Fragaria × ananassa
|
Crown
|
Fragaria x ananassa cv. Albion, Aromas, Camarosa, Camino Real, Monte Rey, Portola, and San Andreas fruits
|
Ultrasonic extraction with acidified methanol
|
Procyanidin dimer and trimer, catechin, propelargonidin dimer, ellagic acid
|
|
DPPH
LC-ESI-TOF
|
IC50 (mg/mL) ranging from 76.73 (Camarosa)—100 (Camino Real)
[21][35]
|
| Total anthocyanin content |
|
| [ | 26][39]
|
Fragaria × ananassa
|
Root
|
Procyanidin dimer and trimer, catechin, neochlorogenic, propelargonidin dimer
|
|
Cytotoxic
|
Fragaria vesca L. leaves
|
Hydroalcoholic extract at room temperature, ellagitannins-enriched fraction
|
Effects on HepG2 cells—cell viability assessment, cell proliferation, cell cycle and cell death analysis, Western blot analysis, proteasome chymotrypsin-like activity
|
Inhibition of HepG2 cell viability IC50 = 690 mg/L (extract)/113 mg/L (fraction); fraction induced necrosis and apoptosis, influenced the cellular proteolytic mechanisms
|
Ellagitannins
|
[19][33LC-ESI-TOF
|
[21][35]
|
] |
|
Chemopreventive
|
Lyophilized Fragaria x ananassa fruits
|
Ultrasound-assisted extraction with acidified acetone
|
Histological studies, Western blot analysis, PGE2 measurement, and nitrate/nitrite colorimetric assay
|
Decreased tumor incidence, decreased levels of TNF-α, IL-1β, IL-6, COX-2 and iNOS, inhibition of the phosphorylation of PI3K, Akt, ERK, and NFκB
|
anthocyanins, ellagitannin/ellagic acid/ellagic acid derivatives flavonols
|
[58][95]
|
F. vesca leaves native to Italy
|
|
Cytotoxic
|
Fragaria x ananassa leaves
|
Hydroalcoholic extracts (meth., eth., isopropanol) from in vitro cell suspension
|
Cell proliferation, cell viability
|
Under 50% viable cells for colorectal adenocarcinoma and colon adenocarcinoma upon treatment with extracts containing 0.29 mM ethoxy-dihydrofuro-furan
|
Polyphenols
|
[59][91]
|
Ultrasonic extraction with ethanol: water solvent (70:30, v/v)
|
TEAC
|
0.34–0.35 mg/mL Trolox eq., compared with quercetin (0.40)
|
Condensed tannins and flavonoid derivatives
|
[28][41]
|
Fragaria × ananassa
|
|
Fragaria x ananassa cv. Tochiotome leaves | Fruits, different novel cultivars
|
|
Supercritical CO2 extraction with different entrainers
Phenolic acids (p-coumaric acid, ellagic acid, ferulic acid derivative, p-coumaric acid derivatives), monomeric flavanols ((+)-catechin), flavonols (quercetin 3-O-glucoside, fisetin, quercetin 3-O-glucoside derivative), anthocyanins (cyanidin 3-glucoside, cyanidin 3-rutinoside, cyanidin pentoside, pelargonidin 3-galactoside, pelargonidin 3,5-diglucoside, pelargonidin 3-glucoside, pelargonidin 3-rutinoside, cyanidin 3-Oacetylglucoside, cyanidin hexoside, pelargonidin 3-O-monoglucuronide, pelargonidin derivatives)
|
|
DPPH
HPLC-DAD, LC-ESI-QTOF
|
0.07 (simple supercritical extraction)—5.82 μmol BHT/g sample (with ethanol, dried at 40 °C)
[22][36]
|
|
| Phenolic compounds |
|
| [40][10]
|
Fragaria × ananassa
|
Fragaria × ananassa fruits (90 cultivars)
|
Fruits, grown on different altitudes, on consecutive years
|
Ultrasonic aqueous methanol (70%) acidified with 1.5% formic acid, at room temperature
Hydroxybenzoic acid, p-coumaric acid, other hydroxycinnamic acids, (+)-catechin, (−)-epicatechin, procyanidins, flavonols, anthocyanins (cyanidin 3-glucoside, pelargonidin 3-glucoside, pelargonidin derivative); higher levels recorded at lower altitudes.
|
DPPH, ABTS
HPLC-DAD
|
[23][37]
|
Average values (μmol Trolox/100 g):765.06 (DPPH), 1637.96 (ABTS) |
|
| Tannin-based compounds. |
|
[31][43]
|
Fragaria × ananassa
|
Fruits
|
Kaempferol 3-(6-methylglucuronide), quercetin 3-(6-methylglucuronide), isorhamnetin 3-(6-methylglucuronide), trichocarpin, 2-p-hydroxybenzoyl-2,4,6-tri hydroxyphenylacetate, 2-p-hydroxyphene thyl-6-caffeoylglucoside, zingerone 4-glucoside, b-hydroxypropiovanillone 3-glucoside, (+)-isolariciresinol 90-glucoside, (−)-isolariciresinol 90-glucoside, aviculin, (−)-secoisolariciresinol 4-glucoside, cupressoside A, cedrusin, icariside E4, dihydrodehydrodiconiferyl alcohol 90-glucoside, massonianoside A, urolignoside, (−)-pinoresinol 4-glucoside, 2,3”-epoxy-4-(butan-2-one-3-yl)-5,7,40-trihydroxy flavane 3-glucoside, kaempferol 3-(6-butylglucuronide), benzyl 2-glucosyl-6-rhamnosylbenzoate
|
1H NMR, 13C NMR, HMBC, HPLC-UV/Vis, LC-MS/MS, HR-ESI-MS,
|
[24][20]
|
F. vesca
|
Fruits, wild and cultivated, from different geographical areas
|
39 phenolic compounds (including cyanidin 3-O-glucoside, delphinidin-3-O-glucoside, pelargonidin-3-O-glucoside, pelargonidin-3-O-rutinoside, (+) catechin, (−) epicatechin, procyanidin B1 and B2, isoquercetin, gallic acid, p-coumaric acid, phloridzin); composition dependent on the geographical area
|
LC-ESI-Orbitrap-MS, LC-ESI-QTrap-MS, LC-ESI-QTrap-MS/MS
|
[25][38]
|
Fragaria × ananassa
|
Fruits, different cultivars
|
Cyanidin 3-O-glucoside, pelargonidin-3-O-glucoside, pelargonidin-O-rutinoside, total anthocyanins content, dependent on the cultivar
|
UPLC-PDA-ESI-MS, HPLC-DAD
|
[26][39]
|
F. vesca
|
Fruits
|
Volatile composition—one hundred compounds (including esters, aldehydes, ketones, alcohols, terpenoids, furans and lactones).
|
GS-MS
|
[27][40]
|
F. vesca
|
Leaves
|
27 metabolites (organic acids, flavonoids, catechin and its oligomers, ellagitannins), including quinic acid, chelidonic acid, quercetin derivatives, catechin and procyanidins, phloridzin, pedunculagin, methyl ellagic acid glucuronide.
|
LC-ESI-Orbitrap-MS
|
[28][41]
|
Fragaria × ananassa, F. vesca
|
White-fruited mutants, different genotypes
|
Anthocyanins, flavonols, flavan-3-ols, hydroxycinnamic acids, and ellagic acid—derived compounds, dependent on genotype
|
LC-ESI-MS/MS
|
[29][21]
|
F. chiloensis
|
Fruits |