1. Structure of Polio Virus

• Poliovirus is a member of a family of viruses called the Picornaviridae.

  Poliovirus is a member of a family of viruses called the Picornaviridae.

• Virions are spherical in shape with a diameter of about 27nm.

  Virions are spherical in shape with a diameter of about 27nm.

• The particles are simple in that they are composed of a protein shell surrounding the naked RNA genome.

  The particles are simple in that they are composed of a protein shell surrounding the naked RNA genome.

• The genome is monopartite, linear ssRNA(+) genome of 7.2-8.5 kb, polyadenylated, composed of a single ORF encoding a polyprotein.

  The genome is monopartite, linear ssRNA(+) genome of 7.2-8.5 kb, polyadenylated, composed of a single ORF encoding a polyprotein.

• The capsids are composed of four structural proteins: VP1, VP2, VP3, and VP4.

  The capsids are composed of four structural proteins: VP1, VP2, VP3, and VP4.

• The basic building block of the picornavirus capsid is the protomer, which contains one copy each of VP1, VP2, VP3, and VP4.

  The basic building block of the picornavirus capsid is the protomer, which contains one copy each of VP1, VP2, VP3, and VP4.

• The shell is formed by VP1 to VP3, and VP4 lies on its inner surface.

  The shell is formed by VP1 to VP3, and VP4 lies on its inner surface.

• The virus particles lack a lipid envelope, and their infectivity is insensitive to organic solvents.

  The virus particles lack a lipid envelope, and their infectivity is insensitive to organic solvents.

2. Genome of Polio Virus

• Polio virus genome can be divided into three parts
  • a 5′ noncoding region (NCR) that comprises approximately 10% of the genome, is uncapped, and is covalently linked at the 5′ terminus to viral protein VPg
  • a single open reading frame that appears to encode all of the viral proteins, with regions designated as P1 for capsid proteins and P2 and P3 for nonstructural proteins
  • a short 3′ NCR terminating in a polyA tail.
• a 5′ noncoding region (NCR) that comprises approximately 10% of the genome, is uncapped, and is covalently linked at the 5′ terminus to viral protein VPg
• a single open reading frame that appears to encode all of the viral proteins, with regions designated as P1 for capsid proteins and P2 and P3 for nonstructural proteins
• a short 3′ NCR terminating in a polyA tail.

• The genomes vary in length from 7,209 to 8,450 bases.

  The genomes vary in length from 7,209 to 8,450 bases.

• The 5′-noncoding region contains the internal ribosome entry site (IRES), an element that directs translation of the mRNA by internal ribosome binding.

  The 5′-noncoding region contains the internal ribosome entry site (IRES), an element that directs translation of the mRNA by internal ribosome binding.

• The regions P1 contains four segments for structural proteins which make up the capsid protein; 1A-VP4, 1B- VP2, 1C-VP3, 1D-VP1.

  The regions P1 contains four segments for structural proteins which make up the capsid protein; 1A-VP4, 1B- VP2, 1C-VP3, 1D-VP1.

• P2 comprises of three non structural proteins; 2A, 2B, 2C which play a role in viral replication.

  P2 comprises of three non structural proteins; 2A, 2B, 2C which play a role in viral replication.

• P3 makes up four non structural proteins

  P3 makes up four non structural proteins

  • 3A- anchors the replication complex to cell membrane

  • 3B- it is VPg protein

  • 3C- it is cysteine protease that cleaves the protein from polypeptides

  • 3D- it is RNA dependent RNA Polymerase.

• 3A- anchors the replication complex to cell membrane
• 3B- it is VPg protein
• 3C- it is cysteine protease that cleaves the protein from polypeptides
• 3D- it is RNA dependent RNA Polymerase

3. Epidemiology of Polio Virus

• Poliomyelitis has had three epidemiologic phases: endemic, epidemic, and the vaccine era.
• Before global eradication efforts began, poliomyelitis occurred worldwide—year-round in the tropics and during summer and fall in the temperate zones.
• Winter outbreaks were rare.
• The disease occurs in all age groups, but children are usually more susceptible than adults because of the acquired immunity of the adult population.
• In developing areas, where living conditions favor the wide dissemination of virus, poliomyelitis is a disease of infancy and early childhood (“infantile paralysis”).
• In developed countries, before the advent of vaccination, the age distribution shifted so that most patients were older than age 5 years, and 25% were older than age 15 years.
• The case fatality rate is variable and is highest in the oldest patients and may reach from 5% to 10%.
• Before the beginning of vaccination campaigns in the United States, there were about 21,000 cases of paralytic poliomyelitis per year.
• Humans are the only known reservoir of infection.
• In temperate zones with high levels of hygiene, epidemics have been followed by periods of little spread of virus until sufficient numbers of susceptible children have grown up to provide a pool for transmission in the area.

• Poliomyelitis has had three epidemiologic phases: endemic, epidemic, and the vaccine era.

• Before global eradication efforts began, poliomyelitis occurred worldwide—year-round in the tropics and during summer and fall in the temperate zones.

• Winter outbreaks were rare.

• The disease occurs in all age groups, but children are usually more susceptible than adults because of the acquired immunity of the adult population.

• In developing areas, where living conditions favor the wide dissemination of virus, poliomyelitis is a disease of infancy and early childhood (“infantile paralysis”).

4. Replication of Polio Virus

• Virus binds to a cellular receptor and the genome is uncoated.
• VPg is removed from the viral RNA, which is then translated.

• In developed countries, before the advent of vaccination, the age distribution shifted so that most patients were older than age 5 years, and 25% were older than age 15 years.

• The case fatality rate is variable and is highest in the oldest patients and may reach from 5% to 10%.

• Before the beginning of vaccination campaigns in the United States, there were about 21,000 cases of paralytic poliomyelitis per year.

• Humans are the only known reservoir of infection.

• In temperate zones with high levels of hygiene, epidemics have been followed by periods of little spread of virus until sufficient numbers of susceptible children have grown up to provide a pool for transmission in the area.

• The polyprotein is cleaved nascently to produce individual viral proteins.
• RNA synthesis occurs on membrane vesicles.
• Viral (+) strand RNA is copied by the viral RNA polymerase to form full-length (–) strand RNAs, which are then copied to produce additional (+) strand RNAs.
• Early in infection, newly synthesized (+) strand RNA is translated to produce additional viral proteins.
• Later in infection, the (+) strands enter the morphogenetic pathway.
• Newly synthesized virus particles are released from the cell by lysis.

• Virus initially multiply in the oropharynx and gastrointestinal mucosa.
• The virus is regularly present in the throat and in the stools before the onset of illness.
• Virions are resistant to acidity of stomach and to lytic activities of the protease and other enzymes of the intestinal tract and bile.
• On entering the body, the virus infects and multiplies in the tonsils and Peyer’s patch of ileum.
• The incubation period is 9-12 days.
• The virus then spreads to regional lymph nodes and enters the blood causing primary viremia.
• Antibodies to the virus appear early in the disease, usually before paralysis occurs.
• The antibodies are produced to prevent infection from spreading.
• On continued infection and multiplication of virus in the ReticuloEndothelial System (RES), it invades the blood stream causing secondary viremia.
• During this period of viremia, the poliovirus crosses the blood brain barrier and gain access to the brain.
• The virus shows tissue tropism by specifically combining with neural cells.
• The virus recognizes the receptor present on the anterior horn of spinal cord, dorsal root ganglia and motor neurons.
• The destruction of motor neurons leads to paralysis.
• The virus also infects brain stem causing bulbar poliomyelitis.

• Virus binds to a cellular receptor and the genome is uncoated.

• VPg is removed from the viral RNA, which is then translated.

• The polyprotein is cleaved nascently to produce individual viral proteins.

• RNA synthesis occurs on membrane vesicles.

• Viral (+) strand RNA is copied by the viral RNA polymerase to form full-length (–) strand RNAs, which are then copied to produce additional (+) strand RNAs.

• Early in infection, newly synthesized (+) strand RNA is translated to produce additional viral proteins.

• Later in infection, the (+) strands enter the morphogenetic pathway.

• Newly synthesized virus particles are released from the cell by lysis.

5. Pathogenesis of Polio Virus

The mouth is the portal of entry for the virus, transmitted by fecal oral route on ingestion of contaminated water.

• The mouth is the portal of entry for the virus, transmitted by fecal oral route on ingestion of contaminated water.

• Virus initially multiply in the oropharynx and gastrointestinal mucosa.

• The virus is regularly present in the throat and in the stools before the onset of illness.

• Virions are resistant to acidity of stomach and to lytic activities of the protease and other enzymes of the intestinal tract and bile.

• During this period of viremia, the poliovirus crosses the blood brain barrier and gain access to the brain.

• The virus shows tissue tropism by specifically combining with neural cells.

• The virus recognizes the receptor present on the anterior horn of spinal cord, dorsal root ganglia and motor neurons.

• The destruction of motor neurons leads to paralysis.

• The virus also infects brain stem causing bulbar poliomyelitis.

6. Clinical Manifestations of Polio Virus

6. Clinical Manifestations of Polio Virus

• The earliest features associated with phase of viremia consist of fever, malaise, headache, drowsiness, constipation, and sore throat and lasts for 1 to 5 days.

• Incubation period is usually 10 days but may vary from 4 days to 4 weeks.

• The earliest features associated with phase of viremia consist of fever, malaise, headache, drowsiness, constipation, and sore throat and lasts for 1 to 5 days.
• Incubation period is usually 10 days but may vary from 4 days to 4 weeks.

Asymptomatic illness

It is caused as a result of viral infection confined to the oropharynx and the intestine.

• It is caused as a result of viral infection confined to the oropharynx and the intestine.

Abortive poliomyelitis

• It is minor illness occurring in approximately 5% of infected people.
• It is febrile illness characterized by fever, headache, sore throat, loss of appetite, vomiting, and abdominal pain.
• Neurological symptoms are typically absent.

• On entering the body, the virus infects and multiplies in the tonsils and Peyer’s patch of ileum.

• The incubation period is 9-12 days.

• The virus then spreads to regional lymph nodes and enters the blood causing primary viremia.

• Antibodies to the virus appear early in the disease, usually before paralysis occurs.

• The antibodies are produced to prevent infection from spreading.

• On continued infection and multiplication of virus in the ReticuloEndothelial System (RES), it invades the blood stream causing secondary viremia.

• Some people who develop symptoms from the poliovirus contract a type of polio that doesn’t lead to paralysis (abortive polio).

• This usually causes the same mild, flu-like signs and symptoms typical of other viral illnesses.

• Signs and symptoms, which can last up to 10 days, include: Fever, sore throat, headache, vomiting, fatigue, back pain or stiffness, neck pain or stiffness, pain or stiffness in the arms or legs and muscle weakness or tenderness.

Paralytic poliomyelitis

• Initial signs and symptoms of paralytic polio, such as fever and headache, often mimic those of non-paralytic polio.
• Within a week, however, other signs and symptoms appear, including: Loss of reflexes, severe muscle aches or weakness and loose and floppy limbs (flaccid paralysis)
• Post poliomyelitis syndrome
• Post-polio syndrome is a cluster of disabling signs and symptoms that affect some people years after having polio.
• Common signs and symptoms include: Progressive muscle or joint weakness and pain, fatigue, muscle wasting (atrophy), breathing or swallowing problems, sleep-related breathing disorder; such as sleep apnea, and decreased tolerance of cold temperatures.

• It is minor illness occurring in approximately 5% of infected people.

• Common signs and symptoms include: Progressive muscle or joint weakness and pain, fatigue, muscle wasting (atrophy), breathing or swallowing problems, sleep-related breathing disorder; such as sleep apnea, and decreased tolerance of cold temperatures.

Bulbar poliomyelitis

• This is caused due to involvement of the cranial nerves, most commonly 9th, 10th, and 12th.
• This condition tends to be more severe with involvement of the muscles of the pharynx, vocal cords and respiration.
• The condition may cause death in 75% of the patient.
• This entry is sourced from https://microbenotes.com/polio-virus/

• It is febrile illness characterized by fever, headache, sore throat, loss of appetite, vomiting, and abdominal pain.

• Neurological symptoms are typically absent.

Non paralytic poliomyelitis

• Some people who develop symptoms from the poliovirus contract a type of polio that doesn’t lead to paralysis (abortive polio).
• This usually causes the same mild, flu-like signs and symptoms typical of other viral illnesses.
• Signs and symptoms, which can last up to 10 days, include: Fever, sore throat, headache, vomiting, fatigue, back pain or stiffness, neck pain or stiffness, pain or stiffness in the arms or legs and muscle weakness or tenderness.

• Initial signs and symptoms of paralytic polio, such as fever and headache, often mimic those of non-paralytic polio.

• Within a week, however, other signs and symptoms appear, including: Loss of reflexes, severe muscle aches or weakness and loose and floppy limbs (flaccid paralysis)

Post poliomyelitis syndrome

• Post-polio syndrome is a cluster of disabling signs and symptoms that affect some people years after having polio.

• This is caused due to involvement of the cranial nerves, most commonly 9^th, 10^th, and 12^th.

• This condition tends to be more severe with involvement of the muscles of the pharynx, vocal cords and respiration.

• The condition may cause death in 75% of the patient.

This entry is sourced from https://microbenotes.com/polio-virus/