C During fetal life, the Eustachian valve on the leftward ridge of the inferior vena cava facilitates the shunting of oxygenated blood from the umbilical vein to the left atrium (LA) via FO. This allows more oxygenated blood to return to the left ventricle (LV). This physiological right to left shunt in fetal life is large, unobstructed, and essential [13]^[6]. At birth, the instant increase in pulmonary blood flow due to the onset of spontaneous ventilation is associated with increased venous return to LA. The distension of the LA with higher volume, along with the decreased vena caval flow into RA after cord clamping, results in the movement of the primum septum rightwards and allows the apposition and subsequent fusion of the primum with the secundum membranes. A deficiency in the membranous portion of the primum and the limbus results in a PFO (Figure 2C). The FO in fetal life can be reliably demonstrated by echocardiography using ultrasound modalities such as 2D, color, and spectral Doppler during the second half of pregnancy [14]^[7]. The fetal FO is however difficult to demonstrate circumferentially despite advances in ultrasound imaging; hence, the transverse diameter is commonly used [14]^[7]. Fetal premature closure or restriction of the FO has been reported at any gestational age with variable effects on fetal hemodynamics and adverse fetal and postnatal outcomes. The spectrum of clinical presentation is related to the gestational age when the restriction first occurs and the severity of the restriction. Significant flow restriction results in an additional amount of blood being re-directed to the right ventricle (RV) as it bypasses the LA, causing right heart failure manifesting as pleural/pericardial effusions, ascites, necrotizing enterocolitis, and non-immune hydrops fetalis. There is also an association between the premature closure of the FO with neonatal mortality and complications such as prematurity, maternal preeclampsia, and placental abruption [15]^[8].

3. PFO in Neonates and Infants

The hemodynamic changes after birth lead to a smooth transition of fetal circulation and primarily depend upon the drop in pulmonary vascular resistance (PVR) and an increase in systemic vascular resistance (SVR). The presence of PFO plays an important role in understanding the normal cardiovascular transition physiology and its adverse adaptation. The shunt across the PFO is either left-to-right or bidirectional but never purely right-to-left. A pure right-to-left shunt across the PFO should raise suspicion of underlying CHDs such as total anomalous pulmonary venous drainage or tricuspid atresia. Assessment of the flow across the PFO, especially when it is bidirectional, may indicate persistent pulmonary hypertension of the newborn, and it contributes to monitoring in premature infants and infants with perinatal asphyxia. In neonates with right heart obstructive lesions, such as tricuspid or pulmonary atresia, and left heart obstructive lesions, such as HLHS, the continued patency of the FO is critical so that an obligatory right-to-left or left-to-right shunt, respectively, across the atrial septum, can take place. In neonates with transposition of the great arteries (TGA), the circulation is parallel (instead of normal in-series circulation), and mixing across the circulations is essential for survival; this is usually provided by the ASD/PFO. With TGA, it is important to assess the atrial level shunt, and a PFO ≤2mm warrants an urgent balloon atrial septostomy to improve survival [21]^[9]. The atrial septum in infants with HLHS can be very thick and, if PFO is restrictive, these newborns become critically ill [22]^[10]. In this case, instead of a balloon atrial septostomy, a blade septostomy or the creation of a separate defect in the atrial septum by Brockenbrough (needle atrial transeptal) puncture or radiofrequency wire perforation is needed.

4. PFO in Childhood

A PFO is frequently detected during childhood by transthoracic echocardiography (TTE) for the evaluation of a murmur or other medical condition. In the majority of children, TTE with or without contrast saline is sufficient to diagnose a PFO because of the excellent acoustic windows [23]^[11]. The most ideal window to visualize the PFO is the subcostal acoustic window. This is because the septum is relatively perpendicular to the transducer and adequately echo-reflective from that position. This reduces the likelihood of a false dropout and a misdiagnosis of a PFO. The confirmation of the PFO is required by documenting evidence of the transseptal flow by color Doppler. Both the subcostal long-axis and short-axis views can be utilized for a confirmatory diagnosis. The apical views often have false dropouts of the acoustic signals due to the parallel orientation of the transducer beam and the atrial septum position. This is not the most ideal location for the evaluation of a PFO. However, the apical-4 chamber view is helpful during the saline contrast “bubble” study in looking for the appearance of acoustic reflective signals (“bubbles”) in the LA and LV (representing right-to-left flow). There is also a need for the standardization of PFO identification and quantification for a saline contrast bubble study, which at present does not exist. In the French PFO- Atrial Septal Aneurysm (ASA) study, a PFO is identified if at least three bubbles appear in the LA. The degree of shunting is defined to be small if three to nine bubbles appear, it is moderate if 10–30 bubbles appear, and large if >30 bubbles appear in the LA [24]^[12]. According to PFO in cryptogenic stroke study (PICSS), a PFO is considered to be present if more than one bubble appears in the LA and if >10 appear in the LA, it is considered a large PFO [25]^[13]. Recently, it was shown that for a given PFO, the amount of right-to-left contrast shunting is a matter of expiratory pressure during the Valsalva maneuver [26]^[14]. Trans-esophageal echocardiogram (TEE) is traditionally considered the gold standard for detection of a PFO in the setting of stroke in adult patients [27,28]^[15][16]. The TEE is necessary for children in order to delineate the anatomy of the interatrial septum during a surgical or interventional cardiac catheterization procedure. In some surgeries, such as tetralogy of Fallot (TOF) repair, a small PFO may be left behind or a punch hole made in the interatrial septum to allow right-to-left shunting and maintain cardiac output in the context of a hypertrophied RV with poor relaxation or potential increased afterload from smallish pulmonary arteries. In these situations, a right-to-left shunt or a bidirectional atrial shunt can be seen on the color Doppler. In other situations, such as a Shone complex with small left-sided structures, there is left-to-right atrial shunting at higher velocities and this evaluation needs to be included in the assessment of transmitral gradients. One major drawback of routine TEE in children is the need for deep sedation or general anesthesia. TEE is a reliable imaging modality to assist with the device closure of a PFO in the cardiac catheterization lab, particularly if a young patient is being anesthetized. In these cases, TEE is useful for determining the precise location (usually the cranial edge of the fossa ovalis), anatomy (slit-like, tunnel-like, aneurysmal or fenestrated), and the direction of shunting, and it can also assist in wire, sheath and device manipulations [29,30,31]^[17][18][19]. TEE is helpful to evaluate rarely for extension of vegetation in endocarditis, thrombus, or left atrial myxoma. TEE is also a useful adjunct to procedures in which the PFO is traversed or dilated or an atrial shunt is created to allow access to the LA to obtain left-sided hemodynamic data and to perform left-sided interventions to the mitral valve, pulmonary vein stents, etc. One critical scenario in which a PFO needs to be looked for is in any kind of dilated cardiomyopathy (Figure 3), in which atrial distension is associated with decreased myocardial perfusion. The presence of a PFO or the creation of an atrial level shunt can decrease atrial distension and encourage myocardial recovery in some cases, especially after the placement of an extracorporeal membrane oxygenator (ECMO) [32]^[20]. Left ventricular assist device (VAD) implantation is another option for some pediatric patients with refractory heart failure. In these patients, the implantation of a VAD abruptly decreases diastolic filling pressures and can cause right-to-left shunting with profound systemic hypoxemia. The pre-VAD TEE diagnosis of a PFO may be challenged by left atrial distension compressing the defect, which may become more apparent when the LA filling pressures decrease after the VAD placement. A PFO is closed at the time of VAD placement if its presence is detected in time; otherwise, device closure may be subsequently needed [33]^[21].