Extrusion Bioprinting for Skin Applications: Comparison
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Please note this is a comparison between Version 2 by Camila Xu and Version 1 by Isabel Andia.

This is an entry focused on extrusion bioprinting for skin applications. Bioprinting technologies have the ability to combine various human cell phenotypes, signaling proteins, extracellular matrix (ECM) components, and other scaffold-like biomaterials and are currently being exploited for the fabrication of human skin, broadly aiming to achieve two main goals. The first goal is to meet the urgent clinical demand for skin equivalents, which can range in complexity from advanced dressings for chronic wounds to biomimetic skin grafts to help restore the barrier function in complex ulcers, burns, or traumatic postsurgical wounds. The second important motivation for skin biofabrication is to create disease models for in vitro research and drug development.

  • bioprinting
  • skin
  • ulcer
  • hydrogel
  • bioink
  • technology readiness level
  • biological therapy
  • tissue engineering
  • regenerative medicine
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References

  1. Braza, M.E.; Fahrenkopf, M.P. Split-Thickness Skin Grafts. In StatPearls; StatPearls Publishing: Treasure Island (FL), 2020.
  2. Andriotis, E.G.; Eleftheriadis, G.K.; Karavasili, C.; Fatouros, D.G. Development of Bio-Active Patches Based on Pectin for the Treatment of Ulcers and Wounds Using 3D-Bioprinting Technology. Pharmaceutics 2020, 12, 56, doi:10.3390/pharmaceutics12010056.
  3. Baltazar, T.; Merola, J.; Catarino, C.; Xie, C.B.; Kirkiles-Smith, N.C.; Lee, V.; Hotta, S.; Dai, G.; Xu, X.; Ferreira, F.C.; et al. Three Dimensional Bioprinting of a Vascularized and Perfusable Skin Graft Using Human Keratinocytes, Fibroblasts, Pericytes, and Endothelial Cells. Tissue Eng. Part A 2020, 26, 227–238, doi:10.1089/ten.tea.2019.0201.
  4. Liu, X.; Michael, S.; Bharti, K.; Ferrer, M.; Song, M.J. A biofabricated vascularized skin model of atopic dermatitis for preclinical studies. Biofabrication 2020, 12, 035002, doi:10.1088/1758-5090/ab76a1.
  5. Wei, Z.; Liu, X.; Ooka, M.; Zhang, L.; Song, M.J.; Huang, R.; Kleinstreuer, N.C.; Simeonov, A.; Xia, M.; Ferrer, M. Two-Dimensional Cellular and Three-Dimensional Bio-Printed Skin Models to Screen Topical-Use Compounds for Irritation Potential. Front. Bioeng. Biotechnol. 2020, 8, 109, doi:10.3389/fbioe.2020.00109.
  6. McCormack, A.; Highley, C.B.; Leslie, N.R.; Melchels, F.P.W. 3D Printing in Suspension Baths: Keeping the Promises of Bioprinting Afloat. Trends Biotechnol. 2020, 38, 584–593, doi:10.1016/j.tibtech.2019.12.020.
  7. Kim, B.S.; Gao, G.; Kim, J.Y.; Cho, D. 3D Cell Printing of Perfusable Vascularized Human Skin Equivalent Composed of Epidermis, Dermis, and Hypodermis for Better Structural Recapitulation of Native Skin. Adv. Healthc. Mater. 2019, 8, 1801019, doi:10.1002/adhm.201801019.
  8. Dussoyer, M.; Courtial, E.J.; Albouy, M.; Thepot, A.; Dos Santos, M.; Marquette, C.A. Mechanical properties of 3D bioprinted dermis: characterization and improvement; Science Repository OÜ, 2019;
  9. Rodrigues, M.; Kosaric, N.; Bonham, C.A.; Gurtner, G.C. Wound Healing: A Cellular Perspective. Physiol. Rev. 2019, 99, 665–706, doi:10.1152/physrev.00067.2017.
  10. Randall, M.J.; Jüngel, A.; Rimann, M.; Wuertz-Kozak, K. Advances in the Biofabrication of 3D Skin in vitro: Healthy and Pathological Models. Front. Bioeng. Biotechnol. 2018, 6, 154, doi:10.3389/fbioe.2018.00154.
  11. Takeo, M.; Lee, W.; Ito, M. Wound Healing and Skin Regeneration. Cold Spring Harb. Perspect. Med. 2015, 5, a023267–a023267, doi:10.1101/cshperspect.a023267.
  12. Andia, I.; Maffulli, N. Platelet-rich plasma for managing pain and inflammation in osteoarthritis. Nat. Rev. Rheumatol. 2013, 9, 721–730, doi:10.1038/nrrheum.2013.141.
  13. Andia, I.; Maffulli, N. A contemporary view of platelet-rich plasma therapies: moving toward refined clinical protocols and precise indications. Regen. Med. 2018, 13, 717–728, doi:10.2217/rme-2018-0042.
  14. de Carvalho, C.K.L.; Fernandes, B.L.; de Souza, M.A. Autologous Matrix of Platelet-Rich Fibrin in Wound Care Settings: A Systematic Review of Randomized Clinical Trials. J. Funct. Biomater. 2020, 11, 31, doi:10.3390/jfb11020031.
  15. del Pino-Sedeño, T.; Trujillo-Martín, M.M.; Andia, I.; Aragón-Sánchez, J.; Herrera-Ramos, E.; Iruzubieta Barragán, F.J.; Serrano-Aguilar, P. Platelet-rich plasma for the treatment of diabetic foot ulcers: A meta-analysis: Platelet-rich plasma for diabetic foot ulcers. Wound Repair Regen. 2019, 27, 170–182, doi:10.1111/wrr.12690.
  16. San Sebastian, K.M.; Lobato, I.; Hernández, I.; Burgos-Alonso, N.; Gomez-Fernandez, M.C.; López, J.L.; Rodríguez, B.; March, A.G.; Grandes, G.; Andia, I. Efficacy and safety of autologous platelet rich plasma for the treatment of vascular ulcers in primary care: Phase III study. BMC Fam. Pract. 2014, 15, 211, doi:10.1186/s12875-014-0211-8.
  17. Perez-Zabala, E.; Basterretxea, A.; Larrazabal, A.; Perez-del-Pecho, K.; Rubio-Azpeitia, E.; Andia, I. Biological approach for the management of non-healing diabetic foot ulcers. J. Tissue Viability 2016, 25, 157–163, doi:10.1016/j.jtv.2016.03.003.
  18. Andia, I.; Abate, M. Platelet-rich plasma: underlying biology and clinical correlates. Regen. Med. 2013, 8, 645–658, doi:10.2217/rme.13.59.
  19. Groll, J.; Burdick, J.A.; Cho, D.-W.; Derby, B.; Gelinsky, M.; Heilshorn, S.C.; Jüngst, T.; Malda, J.; Mironov, V.A.; Nakayama, K.; et al. A definition of bioinks and their distinction from biomaterial inks. Biofabrication 2018, 11, 013001, doi:10.1088/1758-5090/aaec52.
  20. Pisani, S.; Dorati, R.; Scocozza, F.; Mariotti, C.; Chiesa, E.; Bruni, G.; Genta, I.; Auricchio, F.; Conti, M.; Conti, B. Preliminary investigation on a new natural based poly(gamma‐glutamic acid)/Chitosan bioink. J. Biomed. Mater. Res. B Appl. Biomater. 2020, jbm.b.34602, doi:10.1002/jbm.b.34602.
  21. Tigner, T.J.; Rajput, S.; Gaharwar, A.K.; Alge, D.L. Comparison of Photo Cross Linkable Gelatin Derivatives and Initiators for Three-Dimensional Extrusion Bioprinting. Biomacromolecules 2020, 21, 454–463, doi:10.1021/acs.biomac.9b01204.
  22. Zidarič, T.; Milojević, M.; Gradišnik, L.; Stana Kleinschek, K.; Maver, U.; Maver, T. Polysaccharide-Based Bioink Formulation for 3D Bioprinting of an In Vitro Model of the Human Dermis. Nanomaterials 2020, 10, 733, doi:10.3390/nano10040733.
  23. Compaan, A.M.; Song, K.; Huang, Y. Gellan Fluid Gel as a Versatile Support Bath Material for Fluid Extrusion Bioprinting. ACS Appl. Mater. Interfaces 2019, 11, 5714–5726, doi:10.1021/acsami.8b13792.
  24. Motealleh, A.; Dorri, P.; Schäfer, A.H.; Kehr, N.S. 3D bioprinting of triphasic nanocomposite hydrogels and scaffolds for cell adhesion and migration. Biofabrication 2019, 11, 035022, doi:10.1088/1758-5090/ab15ca.
  25. Osidak, E.O.; Karalkin, P.A.; Osidak, M.S.; Parfenov, V.A.; Sivogrivov, D.E.; Pereira, F.D.A.S.; Gryadunova, A.A.; Koudan, E.V.; Khesuani, Y.D.; Кasyanov, V.A.; et al. Viscoll collagen solution as a novel bioink for direct 3D bioprinting. J. Mater. Sci. Mater. Med. 2019, 30, 31, doi:10.1007/s10856-019-6233-y.
  26. Rutz, A.L.; Gargus, E.S.; Hyland, K.E.; Lewis, P.L.; Setty, A.; Burghardt, W.R.; Shah, R.N. Employing PEG crosslinkers to optimize cell viability in gel phase bioinks and tailor post printing mechanical properties. Acta Biomater. 2019, 99, 121–132, doi:10.1016/j.actbio.2019.09.007.
  27. Won, J.-Y.; Lee, M.-H.; Kim, M.-J.; Min, K.-H.; Ahn, G.; Han, J.-S.; Jin, S.; Yun, W.-S.; Shim, J.-H. A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink. Artif. Cells Nanomedicine Biotechnol. 2019, 47, 644–649, doi:10.1080/21691401.2019.1575842.
  28. Pereira, R.F.; Sousa, A.; Barrias, C.C.; Bártolo, P.J.; Granja, P.L. A single-component hydrogel bioink for bioprinting of bioengineered 3D constructs for dermal tissue engineering. Mater. Horiz. 2018, 5, 1100–1111, doi:10.1039/C8MH00525G.
  29. Wang, L.L.; Highley, C.B.; Yeh, Y.-C.; Galarraga, J.H.; Uman, S.; Burdick, J.A. Three-dimensional extrusion bioprinting of single- and double-network hydrogels containing dynamic covalent crosslinks: 3D EXTRUSION BIOPRINTING. J. Biomed. Mater. Res. A 2018, 106, 865–875, doi:10.1002/jbm.a.36323.
  30. Ahn, G.; Min, K.-H.; Kim, C.; Lee, J.-S.; Kang, D.; Won, J.-Y.; Cho, D.-W.; Kim, J.-Y.; Jin, S.; Yun, W.-S.; et al. Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules. Sci. Rep. 2017, 7, 8624, doi:10.1038/s41598-017-09201-5.
  31. Li, H.; Tan, Y.J.; Leong, K.F.; Li, L. 3D Bioprinting of Highly Thixotropic Alginate/Methylcellulose Hydrogel with Strong Interface Bonding. ACS Appl. Mater. Interfaces 2017, 9, 20086–20097, doi:10.1021/acsami.7b04216.
  32. Ouyang, L.; Highley, C.B.; Sun, W.; Burdick, J.A. A Generalizable Strategy for the 3D Bioprinting of Hydrogels from Nonviscous Photo-crosslinkable Inks. Adv. Mater. 2017, 29, 1604983, doi:10.1002/adma.201604983.
  33. Shi, P.; Laude, A.; Yeong, W.Y. Investigation of cell viability and morphology in 3D bio-printed alginate constructs with tunable stiffness: 3D BIO-PRINTED ALGINATE CONSTRUCTS WITH TUNABLE STIFFNESS. J. Biomed. Mater. Res. A 2017, 105, 1009–1018, doi:10.1002/jbm.a.35971.
  34. Dubbin, K.; Hori, Y.; Lewis, K.K.; Heilshorn, S.C. Dual-Stage Crosslinking of a Gel-Phase Bioink Improves Cell Viability and Homogeneity for 3D Bioprinting. Adv. Healthc. Mater. 2016, 5, 2488–2492, doi:10.1002/adhm.201600636.
  35. He, Y.; Yang, F.; Zhao, H.; Gao, Q.; Xia, B.; Fu, J. Research on the printability of hydrogels in 3D bioprinting. Sci. Rep. 2016, 6, 29977, doi:10.1038/srep29977.
  36. Stunova, A.; Vistejnova, L. Dermal fibroblasts—A heterogeneous population with regulatory function in wound healing. Cytokine Growth Factor Rev. 2018, 39, 137–150, doi:10.1016/j.cytogfr.2018.01.003.
  37. Jorgensen, A.M.; Varkey, M.; Gorkun, A.; Clouse, C.; Xu, L.; Chou, Z.; Murphy, S.V.; Molnar, J.; Lee, S.J.; Yoo, J.J.; et al. Bioprinted Skin Recapitulates Normal Collagen Remodeling in Full-Thickness Wounds. Tissue Eng. Part A 2020, 26, 512–526, doi:10.1089/ten.tea.2019.0319.
  38. Kim, B.S.; Kwon, Y.W.; Kong, J.-S.; Park, G.T.; Gao, G.; Han, W.; Kim, M.-B.; Lee, H.; Kim, J.H.; Cho, D.-W. 3D cell printing of in vitro stabilized skin model and in vivo pre-vascularized skin patch using tissue-specific extracellular matrix bioink: A step towards advanced skin tissue engineering. Biomaterials 2018, 168, 38–53, doi:10.1016/j.biomaterials.2018.03.040.
  39. Attalla, R.; Puersten, E.; Jain, N.; Selvaganapathy, P.R. 3D bioprinting of heterogeneous bi- and tri-layered hollow channels within gel scaffolds using scalable multi-axial microfluidic extrusion nozzle. Biofabrication 2018, 11, 015012, doi:10.1088/1758-5090/aaf7c7.
  40. Rutz, A.L.; Hyland, K.E.; Jakus, A.E.; Burghardt, W.R.; Shah, R.N. A Multimaterial Bioink Method for 3D Printing Tunable, Cell-Compatible Hydrogels. Adv. Mater. 2015, 27, 1607–1614, doi:10.1002/adma.201405076.
  41. Shi, Y.; Xing, T.L.; Zhang, H.B.; Yin, R.X.; Yang, S.M.; Wei, J.; Zhang, W.J. Tyrosinase-doped bioink for 3D bioprinting of living skin constructs. Biomed. Mater. 2018, 13, 035008, doi:10.1088/1748-605X/aaa5b6.
  42. Werner, S.; Krieg, T.; Smola, H. Keratinocyte–Fibroblast Interactions in Wound Healing. J. Invest. Dermatol. 2007, 127, 998–1008, doi:10.1038/sj.jid.5700786.
  43. Albanna, M.; Binder, K.W.; Murphy, S.V.; Kim, J.; Qasem, S.A.; Zhao, W.; Tan, J.; El-Amin, I.B.; Dice, D.D.; Marco, J.; et al. In Situ Bioprinting of Autologous Skin Cells Accelerates Wound Healing of Extensive Excisional Full-Thickness Wounds. Sci. Rep. 2019, 9, 1856, doi:10.1038/s41598-018-38366-w.
  44. Hakimi, N.; Cheng, R.; Leng, L.; Sotoudehfar, M.; Ba, P.Q.; Bakhtyar, N.; Amini-Nik, S.; Jeschke, M.G.; Günther, A. Handheld skin printer: in situ formation of planar biomaterials and tissues. Lab. Chip 2018, 18, 1440–1451, doi:10.1039/C7LC01236E.
  45. Cubo, N.; Garcia, M.; del Cañizo, J.F.; Velasco, D.; Jorcano, J.L. 3D bioprinting of functional human skin: production and in vivo analysis. Biofabrication 2016, 9, 015006, doi:10.1088/1758-5090/9/1/015006.
  46. Seol, Y.-J.; Lee, H.; Copus, J.S.; Kang, H.-W.; Cho, D.-W.; Atala, A.; Lee, S.J.; Yoo, J.J. 3D bioprinted biomask for facial skin reconstruction. Bioprinting 2018, 10, e00028, doi:10.1016/j.bprint.2018.e00028.
  47. Kim, B.S.; Lee, J.-S.; Gao, G.; Cho, D.-W. Direct 3D cell-printing of human skin with functional transwell system. Biofabrication 2017, 9, 025034, doi:10.1088/1758-5090/aa71c8.
  48. Harrell, C.; Fellabaum, C.; Jovicic, N.; Djonov, V.; Arsenijevic, N.; Volarevic, V. Molecular Mechanisms Responsible for Therapeutic Potential of Mesenchymal Stem Cell-Derived Secretome. Cells 2019, 8, 467, doi:10.3390/cells8050467.
  49. Kajave, N.S.; Schmitt, T.; Nguyen, T.-U.; Kishore, V. Dual crosslinking strategy to generate mechanically viable cell-laden printable constructs using methacrylated collagen bioinks. Mater. Sci. Eng. C 2020, 107, 110290, doi:10.1016/j.msec.2019.110290.
  50. Liu, P.; Shen, H.; Zhi, Y.; Si, J.; Shi, J.; Guo, L.; Shen, S.G. 3D bioprinting and in vitro study of bilayered membranous construct with human cells-laden alginate/gelatin composite hydrogels. Colloids Surf. B Biointerfaces 2019, 181, 1026–1034, doi:10.1016/j.colsurfb.2019.06.069.
  51. Montheil, T.; Maumus, M.; Valot, L.; Lebrun, A.; Martinez, J.; Amblard, M.; Noël, D.; Mehdi, A.; Subra, G. Inorganic Sol–Gel Polymerization for Hydrogel Bioprinting. ACS Omega 2020, 5, 2640–2647, doi:10.1021/acsomega.9b03100.
  52. Xin, S.; Chimene, D.; Garza, J.E.; Gaharwar, A.K.; Alge, D.L. Clickable PEG hydrogel microspheres as building blocks for 3D bioprinting. Biomater. Sci. 2019, 7, 1179–1187, doi:10.1039/C8BM01286E.
  53. Giuseppe, M.D.; Law, N.; Webb, B.; A. Macrae, R.; Liew, L.J.; Sercombe, T.B.; Dilley, R.J.; Doyle, B.J. Mechanical behaviour of alginate-gelatin hydrogels for 3D bioprinting. J. Mech. Behav. Biomed. Mater. 2018, 79, 150–157, doi:10.1016/j.jmbbm.2017.12.018.
  54. Law, N.; Doney, B.; Glover, H.; Qin, Y.; Aman, Z.M.; Sercombe, T.B.; Liew, L.J.; Dilley, R.J.; Doyle, B.J. Characterisation of hyaluronic acid methylcellulose hydrogels for 3D bioprinting. J. Mech. Behav. Biomed. Mater. 2018, 77, 389–399, doi:10.1016/j.jmbbm.2017.09.031.
  55. Sasaki, M.; Abe, R.; Fujita, Y.; Ando, S.; Inokuma, D.; Shimizu, H. Mesenchymal Stem Cells Are Recruited into Wounded Skin and Contribute to Wound Repair by Transdifferentiation into Multiple Skin Cell Type. J. Immunol. 2008, 180, 2581–2587, doi:10.4049/jimmunol.180.4.2581.
  56. Chu, D.-T.; Nguyen Thi Phuong, T.; Tien, N.L.B.; Tran, D.K.; Minh, L.B.; Thanh, V.V.; Gia Anh, P.; Pham, V.H.; Thi Nga, V. Adipose Tissue Stem Cells for Therapy: An Update on the Progress of Isolation, Culture, Storage, and Clinical Application. J. Clin. Med. 2019, 8, 917, doi:10.3390/jcm8070917.
  57. Mendes, B.B.; Gómez-Florit, M.; Hamilton, A.G.; Detamore, M.S.; Domingues, R.M.A.; Reis, R.L.; Gomes, M.E. Human platelet lysate-based nanocomposite bioink for bioprinting hierarchical fibrillar structures. Biofabrication 2019, 12, 015012, doi:10.1088/1758-5090/ab33e8.
  58. Pepelanova, I.; Kruppa, K.; Scheper, T.; Lavrentieva, A. Gelatin-Methacryloyl (GelMA) Hydrogels with Defined Degree of Functionalization as a Versatile Toolkit for 3D Cell Culture and Extrusion Bioprinting. Bioengineering 2018, 5, 55, doi:10.3390/bioengineering5030055.
  59. Raddatz, L.; Lavrentieva, A.; Pepelanova, I.; Bahnemann, J.; Geier, D.; Becker, T.; Scheper, T.; Beutel, S. Development and Application of an Additively Manufactured Calcium Chloride Nebulizer for Alginate 3D-Bioprinting Purposes. J. Funct. Biomater. 2018, 9, 63, doi:10.3390/jfb9040063.
  60. Ouyang, L.; Yao, R.; Zhao, Y.; Sun, W. Effect of bioink properties on printability and cell viability for 3D bioplotting of embryonic stem cells. Biofabrication 2016, 8, 035020, doi:10.1088/1758-5090/8/3/035020.
  61. Li, Z.; Huang, S.; Liu, Y.; Yao, B.; Hu, T.; Shi, H.; Xie, J.; Fu, X. Tuning Alginate-Gelatin Bioink Properties by Varying Solvent and Their Impact on Stem Cell Behavior. Sci. Rep. 2018, 8, 8020, doi:10.1038/s41598-018-26407-3.
  62. Li, Y.; Jiang, X.; Li, L.; Chen, Z.-N.; Gao, G.; Yao, R.; Sun, W. 3D printing human induced pluripotent stem cells with novel hydroxypropyl chitin bioink: scalable expansion and uniform aggregation. Biofabrication 2018, 10, 044101, doi:10.1088/1758-5090/aacfc3.
  63. 3D Bioprinting: Principles and Protocols; Crook, J.M., Ed.; Methods in Molecular Biology; Springer US: New York, NY, 2020; Vol. 2140; ISBN 978-1-07-160519-6.
  64. Pu, L.; Meng, M.; Wu, J.; Zhang, J.; Hou, Z.; Gao, H.; Xu, H.; Liu, B.; Tang, W.; Jiang, L.; et al. Compared to the amniotic membrane, Wharton’s jelly may be a more suitable source of mesenchymal stem cells for cardiovascular tissue engineering and clinical regeneration. Stem Cell Res. Ther. 2017, 8, 72, doi:10.1186/s13287-017-0501-x.
  65. Skardal, A.; Mack, D.; Kapetanovic, E.; Atala, A.; Jackson, J.D.; Yoo, J.; Soker, S. Bioprinted Amniotic Fluid-Derived Stem Cells Accelerate Healing of Large Skin Wounds. STEM CELLS Transl. Med. 2012, 1, 792–802, doi:10.5966/sctm.2012-0088.
  66. Liu, W.; Heinrich, M.A.; Zhou, Y.; Akpek, A.; Hu, N.; Liu, X.; Guan, X.; Zhong, Z.; Jin, X.; Khademhosseini, A.; et al. Extrusion Bioprinting of Shear‐Thinning Gelatin Methacryloyl Bioinks. Adv. Healthc. Mater. 2017, 6, 1601451, doi:10.1002/adhm.201601451.
  67. Derr, K.; Zou, J.; Luo, K.; Song, M.J.; Sittampalam, G.S.; Zhou, C.; Michael, S.; Ferrer, M.; Derr, P. Fully Three-Dimensional Bioprinted Skin Equivalent Constructs with Validated Morphology and Barrier Function. Tissue Eng. Part C Methods 2019, 25, 334–343, doi:10.1089/ten.tec.2018.0318.
  68. Anitua, E.; Sánchez, M.; Orive, G.; Andia, I. Delivering growth factors for therapeutics. Trends Pharmacol. Sci. 2008, 29, 37–41, doi:10.1016/j.tips.2007.10.010.
  69. Quílez, C.; de Aranda Izuzquiza, G.; García, M.; López, V.; Montero, A.; Valencia, L.; Velasco, D. Bioprinting for Skin. In 3D Bioprinting: Principles and Protocols; Crook, J.M., Ed.; Methods in Molecular Biology; Springer US: New York, NY, 2020; pp. 217–228 ISBN 978-1-07-160520-2.
  70. Choudhury, D.; Tun, H.W.; Wang, T.; Naing, M.W. Organ-Derived Decellularized Extracellular Matrix: A Game Changer for Bioink Manufacturing? Trends Biotechnol. 2018, 36, 787–805, doi:10.1016/j.tibtech.2018.03.003.
  71. Wu, Z.; Su, X.; Xu, Y.; Kong, B.; Sun, W.; Mi, S. Bioprinting three-dimensional cell-laden tissue constructs with controllable degradation. Sci. Rep. 2016, 6, 24474, doi:10.1038/srep24474.
  72. Moreno-Arotzena, O.; Meier, J.; del Amo, C.; García-Aznar, J. Characterization of Fibrin and Collagen Gels for Engineering Wound Healing Models. Materials 2015, 8, 1636–1651, doi:10.3390/ma8041636.
  73. Del Amo, C.; Borau, C.; Movilla, N.; Asín, J.; García-Aznar, J.M. Quantifying 3D chemotaxis in microfluidic-based chips with step gradients of collagen hydrogel concentrations. Integr. Biol. 2017, 9, 339–349, doi:10.1039/c7ib00022g.
  74. Del Amo, C.; Perez-Valle, A.; Perez-Zabala, E.; Perez-del-Pecho, K.; Larrazabal, A.; Basterretxea, A.; Bully, P.; Andia, I. Wound Dressing Selection Is Critical to Enhance Platelet-Rich Fibrin Activities in Wound Care. Int. J. Mol. Sci. 2020, 21, 624, doi:10.3390/ijms21020624.
  75. Unagolla, J.M.; Jayasuriya, A.C. Enhanced cell functions on graphene oxide incorporated 3D printed polycaprolactone scaffolds. Mater. Sci. Eng. C 2019, 102, 1–11, doi:10.1016/j.msec.2019.04.026.
  76. Panwar, A.; Tan, L. Current Status of Bioinks for Micro-Extrusion-Based 3D Bioprinting. Molecules 2016, 21, 685, doi:10.3390/molecules21060685.
  77. Ooi, H.W.; Mota, C.; ten Cate, A.T.; Calore, A.; Moroni, L.; Baker, M.B. Thiol–Ene Alginate Hydrogels as Versatile Bioinks for Bioprinting. Biomacromolecules 2018, 19, 3390–3400, doi:10.1021/acs.biomac.8b00696.
  78. Lee, H.J.; Kim, Y.B.; Ahn, S.H.; Lee, J.-S.; Jang, C.H.; Yoon, H.; Chun, W.; Kim, G.H. A New Approach for Fabricating Collagen/ECM-Based Bioinks Using Preosteoblasts and Human Adipose Stem Cells. Adv. Healthc. Mater. 2015, 4, 1359–1368, doi:10.1002/adhm.201500193.
  79. Bociaga, D.; Bartniak, M.; Grabarczyk, J.; Przybyszewska, K. Sodium Alginate/Gelatine Hydrogels for Direct Bioprinting—The Effect of Composition Selection and Applied Solvents on the Bioink Properties. Materials 2019, 12, 2669, doi:10.3390/ma12172669.
  80. Gungor-Ozkerim, P.S.; Inci, I.; Zhang, Y.S.; Khademhosseini, A.; Dokmeci, M.R. Bioinks for 3D bioprinting: an overview. Biomater. Sci. 2018, 6, 915–946, doi:10.1039/C7BM00765E.
  81. Yue, K.; Trujillo-de Santiago, G.; Alvarez, M.M.; Tamayol, A.; Annabi, N.; Khademhosseini, A. Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. Biomaterials 2015, 73, 254–271, doi:10.1016/j.biomaterials.2015.08.045.
  82. Alves, L.; Medronho, B.; Antunes, F.E.; Fernández-García, M.P.; Ventura, J.; Araújo, J.P.; Romano, A.; Lindman, B. Unusual extraction and characterization of nanocrystalline cellulose from cellulose derivatives. J. Mol. Liq. 2015, 210, 106–112, doi:10.1016/j.molliq.2014.12.010.
  83. Turley, E.A.; Noble, P.W.; Bourguignon, L.Y.W. Signaling Properties of Hyaluronan Receptors. J. Biol. Chem. 2002, 277, 4589–4592, doi:10.1074/jbc.R100038200.
  84. Baier, C.; Baader, S.L.; Jankowski, J.; Gieselmann, V.; Schilling, K.; Rauch, U.; Kappler, J. Hyaluronan is organized into fiber-like structures along migratory pathways in the developing mouse cerebellum. Matrix Biol. 2007, 26, 348–358, doi:10.1016/j.matbio.2007.02.002.
  85. Ozbolat, I.T.; Hospodiuk, M. Current advances and future perspectives in extrusion-based bioprinting. Biomaterials 2016, 76, 321–343, doi:10.1016/j.biomaterials.2015.10.076.
  86. Aya, K.L.; Stern, R. Hyaluronan in wound healing: Rediscovering a major player: Hyaluronan in wound healing. Wound Repair Regen. 2014, 22, 579–593, doi:10.1111/wrr.12214.
  87. Wu, C.; Wang, B.; Zhang, C.; Wysk, R.A.; Chen, Y.-W. Bioprinting: an assessment based on manufacturing readiness levels. Crit. Rev. Biotechnol. 2017, 37, 333–354, doi:10.3109/07388551.2016.1163321.
  88. MedicalCountermeasures.gov Available online: https://www.medicalcountermeasures.gov/trl/integrated-trls/ (accessed on Aug 31, 2020).
  89. Naveau, A.; Smirani, R.; Catros, S.; de Oliveira, H.; Fricain, J.-C.; Devillard, R. A Bibliometric Study to Assess Bioprinting Evolution. Appl. Sci. 2017, 7, 1331, doi:10.3390/app7121331.
  90. Costa, P.F. Translating Biofabrication to the Market. Trends Biotechnol. 2019, 37, 1032–1036, doi:10.1016/j.tibtech.2019.04.013.
  91. Yang, Q.; Gao, B.; Xu, F. Recent Advances in 4D Bioprinting. Biotechnol. J. 2020, 15, 1900086, doi:10.1002/biot.201900086.
  92. Wu, Y.; Ravnic, D.J.; Ozbolat, I.T. Intraoperative Bioprinting: Repairing Tissues and Organs in a Surgical Setting. Trends Biotechnol. 2020, 38, 594–605, doi:10.1016/j.tibtech.2020.01.004.
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